Proximate Composition, Phytochemical and Elemental Analysis of Some Organic Solvent Extract of The Wild Mushroom- Ganoderma lucidum.

Brief about the title: The crude extract powder of Ganoderma lucidum harvested from Lafia, Nassarawa State of Nigeria during the rainy season was analyzed for proximate contents, phytochemical constituents and mineral composition using various standard methods. Description of the experiment and procedures: The harvested Ganoderma lucidum was air dried at 370C and grinded to powder; this was preliminary analyzed for proximate contents, phytochemical constituents. The crude powder was subjected to soxhlet extraction at 400C using Methanol, Ethylacetate and N-butanol to obtain different organic solvent fractions, these were then concentrated in vacuo at 240C for 48 hours to obtain different solvent extract fractions. These extracts were then analyzed for phytochemical contents using standards methods. Results: Analysis for proximate constituent showed Moisture contents was 10.54%, Total ash 5.93%, Protein 17.55%, Crude Fats 2.60%, Crude Fiber 30.25%, Carbohydrates 33.13%, and Nitrogen 23.52%. Phytochemical screening from unprocessed G.lucidum powder reveals the presence of:  Alkaloids, Flavonoids, Reducing sugars, Tannins, Cardiac glycosides, Anthraquinones, Saponins, Volatile oils and Steroids. However, variations in the presence and concentrations of these phytochemicals were observed in the partitioned portions separated by Methanol, Ethyl acetate and N-butanol. Analysis of Total ash reveals in (mg/kg) the presence of Ca (322.6), K (317.1), P (197.1), and Na (193.5) in high quantity, while C (68.2), Fe (44.6), and Zn (14.65) and Mg (8.7.0), were found to be in moderate quantity. However, Si (4.10), Mn (1.83), As (1.23), Cu (0.84), Cr (0.14), Pb (0.106), Mo (0.09), Ni (0.095), F (0.0039), Al (0.20) and Co (0.026) are very low in concentration, but Se (0.00) was completely not detected. Conclusion: The presence of these essential nutrients and minerals found in G.lucidum implies that it can be utilized for its medicinal values in healthcare delivery systems, and the medicinal importance are thus highlighted in this work. Keywords: Ganoderma lucidum, Fruiting body, proximate analysis, Phytochemical and Mineral, Healthcar

Interaction Between Hot Carrier Aging and PBTI Degradation in nMOSFETs: Characterization, Modelling and Lifetime Prediction

Modelling of the interaction between Hot Carrier Aging (HCA) and Positive Bias Temperature Instability (PBTI) has been considered as one of the main challenges in nanoscale CMOS circuit design. Previous works were mainly based on separate HCA and PBTI instead of Interacted HCA-PBTI Degradation (IHPD). The key advance of this work is to develop a methodology that enables accurate modelling of IHPD through understanding the charging/discharging and generation kinetics of different types of defects during the interaction between HCA and PBTI. It is found that degradation during alternating HCA and PBTI stress cannot be modelled by independent HCI/PBTI. Different stress sequence, i.e. HCA-PBTI-HCA and PBTI-HCA-PBTI, lead to completely different degradation kinetics. Based on the Cyclic Anti-neutralization Model (CAM), for the first time, IHPD has been accurately modelled for both short and long channel devices. Complex degradation mechanisms and kinetics can be well explained by our model. Our results show that device lifetime can be underestimated by one decade without considering interaction

Process and outcome-based evaluation between virtual really-driven and traditional construction safety training

The emerging digital technologies such as virtual reality (VR) provide an alternative platform for construction safety training. In order to explore how digital-driven technologies affect the effectiveness of safety training, there is a need to empirically test the differences in performance between digital 3D/VR safety training and traditional 2D/paper approach. This research conducted a performance evaluation that emphasises both the training process and learning outcomes of trainees based on researchers’ self-developed immersive construction safety training platform. Data related to physiological indicators such as skin resistance were collected to measure safety performance before and after the training. The detailed measurement indicators included nine categories (e.g., immersion, inspiration) to form a holistic list of evaluation dimensions. The findings revealed that VR-driven immersive safety training outperformed the traditional way for trainees in terms of both process and outcome-based indicators. Results confirmed that safety training was no longer constrained by understanding or memorizing 2D information (texts and images). Instead, trainees experienced a stronger sense of embodied cognition through the immersive experience and multi-sensory engagement by interacting with the VR-driven system. By engaging the theory of embodied cognition, this research provides both the empirical evidence and in-depth analysis of how immersive virtual safety training outperforms traditional training in terms of both training process and outcomes.National Natural Science Foundation of China (Grant No. 72071097); Humanities and Social Science Fund of Ministry of Education of China (CN) (Grant No. 20YJAZH034); 16th Talent Summit Program of Six Major Fields in Jiangsu Province (Grant No. SZCY-014)

Fingerprint White Line Counts: An Upcoming Forensic Tool for Sex Determination

Abstract Fingerprints are one of the common forensic tools used in personal identification. However, the associated secondary epidermal creases of fingerprints, fingerprint white line count (FWLC), has received less attention within the forensic community. This study was conducted with an aim to determine the potential of FWLC in sex inference among adult Nigerians. A cross sectional study was carried out with 150 males and 150 females with age range of 18-30 and 18-33 years, respectively. Live scanner (Digita persona, China) was used to capture the plain fingerprint for FWLC. Mann Whitney, Kruskal Wallis and logistic regression analyses were employed for determination of digit variation (based on side and type), sexual dimorphism and prediction models, respectively. Likelihood ratio and posterior probability were used to determine the favour odd for sex inference from FWLC. A significant higher mean value of FWLC was observed in females (2.24 ± 2.03) compared to males (0.85 ± 1.29). Absence of white line was indicative of male origin in all the digits except for left index digit (favor odd of 0.72 for females and 0.29 for males). However, FWLC from 5 to 11 were more likely to be of female origin. The best discriminator of sex was the left FWLC with a percentage accuracy of discrimination of 72%. The percentage contribution of the left FWLC in the discrimination of the sexes was observed to range from 23.0 to 30.20%. The FWLC was found to be a potential predictor of sex among adult Nigerians of Hausa ethnic origin

Potential and Accuracy of Hand Length and Hand Breadth in Sex Determination: An Insight into Hausa Population of Nigeria

The identification of an individual in mass disasters and traffic accidents is a fundamental goal in forensic investigations. However, it is suggested that every population needs anthropological reference data. The objectives of this study were to determine the potential and accuracy of hand length and hand breadth in sex determination among the Hausa population of Nigeria. Random sampling was employed to select 204 male and 194 female subjects aged 18-30 years. Hand length and hand breadth were measured using standard protocols. Two-sample t-test, binary logistic regression, receiver operating characteristics curve, and Youden’s index were used for determining sex using hand dimensions. Posterior probability and likelihood ratio were used to determine the favor odds of each category of hand dimension in sex discrimination. A significant sexual dimorphism was observed in hand length and hand breadth with higher mean values in males. The variance of sex explained by hand parameters ranges from 38.50/51.35% (lower/upper limits) to 52.98/70.66%, which were exhibited by right hand length and left hand breadth respectively. The overall prediction and percentage accuracy of sex discrimination ranges between 80.40 to 86.70 % that were exhibited by right hand length and handbreadth respectively. We observed that left hand breadth exhibited higher Younden’s index with an area under curve (AUC) ranging from 91.60% to 96.05%. We found that hand length of > 189.99 mm was indicative of male origin and hand breadth of > 79.99 mm was indicative of male origin

Chronic Hepatitis B Virus Infection and Rubella Susceptibility Among Obstetric Population in Metropolis Antenatal Centre Kano, Nigeria

It is well known that hepatitis B virus (HBV) infection is endemic in Nigeria. However, increased rubella susceptibility has been shown in patients from the Asian pacific region where chronic HBV infection is endemic. This study was carried out to assess the relationship between chronic HBV infection and rubella susceptibility in obstetric population aged 15–47 years attending Antenatal Clinic at Muhammad Abdullahi Wase Specialist Hospital Kano, Nigeria. From a total of 288 patients screened, 31 (10.76%) were reactive for HBsAg, meanwhile 50 (17.36%) were reactive to rubella IgM. Among the 31 infected patients 15 (48.39%) were from 20 – 24 years age bracket representing the most  susceptible age group while the infection rate was lowest (0.35%) in 45 – 49  age group (P = 0.00). The results of serological markers shows that HBsAg (+) was found in all 31 subjects (100%), anti HBs (+) 0 (0.00%), HBeAg (+) 3 (9.68%); anti HBe (+) and anti HBc (+) 24 (77.42%) respectively (P = 0.09). The study of liver enzymes activity among the HBV positive patients shows abnormal Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) among HBsAg (+) and HBeAg (+) group. However, abnormal Alkaline phospatase (ALP) was found to be non-significantly different between HBsAg (+) and HBeAg (+) vsHBsAg (+) and HBeAg (-) groups (P=0.00). Moreover, obstetric histories such as abortion still birth and neonatal deaths among various age groups with respect to rubella was also studied, it implies that  out of the 50 reactive patients, 35(12.15%) had a previous abnormal obstetric history (P=0.02). In a comparative study conducted, it was observed that HBV carriers were (25.81%) susceptible to rubella as against (12.91%) observed in HBV free subjects (positive correlation). The study demonstrates strong associations between chronic HBV infection and rubella susceptibility among the studied population

Preparing for and executing a randomised controlled trial of podoconiosis treatment in Northern Ethiopia: the utility of rapid ethical assessment

Background Community-based randomized controlled trials are often complex pieces of research with significant challenges around the approach to the community, information provision, and decision-making, all of which are fundamental to the informed consent process. We conducted a rapid ethical assessment to guide the preparation for and conduct of a randomized controlled trial of podoconiosis treatment in northern Ethiopia. Methods A qualitative study was carried out in Aneded woreda, East Gojjam Zone, Amhara Regional State from August to September, 2013. A total of 14 In-depth Interviews (IDIs) with researchers, experts, and leaders, and 8 Focus Group Discussions (FGDs) involving 80 participants (people of both gender, with and without podoconiosis), were conducted. Interviews were carried out in Amharic. Data analysis was started alongside collection. Final data analysis used a thematic approach based on themes identified a priori and those that emerged during the analysis. Results Respondents made a range of specific suggestions, including that sensitisation meetings were called by woreda or kebele leaders or the police; that Health Extension Workers were asked to accompany the research team to patients’ houses; that detailed trial information was explained by someone with deep local knowledge; that analogies from agriculture and local social organisations be used to explain randomisation; that participants in the ‘delayed’ intervention arm be given small incentives to continue in the trial; and that key community members be asked to quell rumours arising in the course of the trial. Conclusion Many of these recommendations were incorporated into the preparatory phases of the trial, or were used during the course of the trial itself. This demonstrates the utility of rapid ethical assessment preceding a complex piece of research in a relatively research-naive setting

Molecular characterisation of protist parasites in human-habituated mountain gorillas (Gorilla beringei beringei), humans and livestock, from Bwindi impenetrable National Park, Uganda

Over 60 % of human emerging infectious diseases are zoonotic, and there is growing evidence of the zooanthroponotic transmission of diseases from humans to livestock and wildlife species, with major implications for public health, economics, and conservation. Zooanthroponoses are of relevance to critically endangered species; amongst these is the mountain gorilla (Gorilla beringei beringei) of Uganda. Here, we assess the occurrence of Cryptosporidium, Cyclospora, Giardia, and Entamoeba infecting mountain gorillas in the Bwindi Impenetrable National Park (BINP), Uganda, using molecular methods. We also assess the occurrence of these parasites in humans and livestock species living in overlapping/adjacent geographical regions

Traditional values of virginity and sexual behaviour in rural Ethiopian youth: results from a cross-sectional study

<p>Abstract</p> <p>Background</p> <p>Delaying sexual initiation has been promoted as one of the methods of decreasing risks of HIV among young people. In traditional countries, such as Ethiopia, retaining virginity until marriage is the norm. However, no one has examined the impact of this traditional norm on sexual behaviour and risk of HIV in marriage. This study examined the effect of virginity norm on having sex before marriage and sexual behaviour after marriage among rural Ethiopian youth.</p> <p>Methods</p> <p>We did a cross-sectional survey in 9 rural and 1 urban area using a probabilistic sample of 3,743 youth, 15–24 years of age. Univariate analysis was used to assess associations between virginity norm and gender stratified by area, and between sexual behaviour and marital status. We applied Kaplan-Meier and Cox regression analysis to estimate age at sexual debut and assessed the predictors of premarital sex among the never-married using SPSS.</p> <p>Results</p> <p>We found that maintaining virginity is still a way of securing marriage for girls, especially in rural areas; the odds of belief and intention to marry a virgin among boys was 3–4 times higher among rural young males. As age increased, the likelihood of remaining a virgin decreased. There was no significant difference between married and unmarried young people in terms of number of partners and visiting commercial sex workers. Married men were twice more likely to have multiple sexual partners than their female counterparts. A Cox regression show that those who did not believe in traditional values of preserving virginity (adjusted hazard ratio [AHR] = 2.91 [1.92–4.40]), alcohol drinkers (AHR = 2.91 [1.97–4.29]), Khat chewers (AHR = 2.36 [1.45–3.85]), literates (AHR = 18.01 [4.34–74.42]), and the older age group (AHR = 1.85 [1.19–2.91]) were more likely to have premarital sex than their counterparts.</p> <p>Conclusion</p> <p>Although virginity norms help delay age at sexual debut among rural Ethiopian youth, and thus reduces vulnerability to sexually transmitted infections and HIV infection, vulnerability among females may increase after marriage due to unprotected multiple risky sexual behaviours by spouses. The use of preventive services, such as VCT before marriage and condom use in marriage should be part of the HIV/AIDS prevention and control strategies.</p

Measuring routine childhood vaccination coverage in 204 countries and territories, 1980–2019 : a systematic analysis for the Global Burden of Disease Study 2020, Release 1

Background: Measuring routine childhood vaccination is crucial to inform global vaccine policies and programme implementation, and to track progress towards targets set by the Global Vaccine Action Plan (GVAP) and Immunization Agenda 2030. Robust estimates of routine vaccine coverage are needed to identify past successes and persistent vulnerabilities. Drawing from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2020, Release 1, we did a systematic analysis of global, regional, and national vaccine coverage trends using a statistical framework, by vaccine and over time. Methods: For this analysis we collated 55 326 country-specific, cohort-specific, year-specific, vaccine-specific, and dose-specific observations of routine childhood vaccination coverage between 1980 and 2019. Using spatiotemporal Gaussian process regression, we produced location-specific and year-specific estimates of 11 routine childhood vaccine coverage indicators for 204 countries and territories from 1980 to 2019, adjusting for biases in country-reported data and reflecting reported stockouts and supply disruptions. We analysed global and regional trends in coverage and numbers of zero-dose children (defined as those who never received a diphtheria-tetanus-pertussis [DTP] vaccine dose), progress towards GVAP targets, and the relationship between vaccine coverage and sociodemographic development. Findings: By 2019, global coverage of third-dose DTP (DTP3; 81·6% [95% uncertainty interval 80·4–82·7]) more than doubled from levels estimated in 1980 (39·9% [37·5–42·1]), as did global coverage of the first-dose measles-containing vaccine (MCV1; from 38·5% [35·4–41·3] in 1980 to 83·6% [82·3–84·8] in 2019). Third-dose polio vaccine (Pol3) coverage also increased, from 42·6% (41·4–44·1) in 1980 to 79·8% (78·4–81·1) in 2019, and global coverage of newer vaccines increased rapidly between 2000 and 2019. The global number of zero-dose children fell by nearly 75% between 1980 and 2019, from 56·8 million (52·6–60·9) to 14·5 million (13·4–15·9). However, over the past decade, global vaccine coverage broadly plateaued; 94 countries and territories recorded decreasing DTP3 coverage since 2010. Only 11 countries and territories were estimated to have reached the national GVAP target of at least 90% coverage for all assessed vaccines in 2019. Interpretation: After achieving large gains in childhood vaccine coverage worldwide, in much of the world this progress was stalled or reversed from 2010 to 2019. These findings underscore the importance of revisiting routine immunisation strategies and programmatic approaches, recentring service delivery around equity and underserved populations. Strengthening vaccine data and monitoring systems is crucial to these pursuits, now and through to 2030, to ensure that all children have access to, and can benefit from, lifesaving vaccines