Comparison of components and anti-liver cancer activity in vitro between Huanglian and Yunlian

Objective: Coptidis Rhizoma is a famous Chinese medicinal herb for its potential to combat cancer, inflammation, fibrosis, viral and bacterial infections, and oxidation. Coptis chinensis Franch (Huanglian in Chinese HL) and Coptis teeta Wall (Yun Huanglian or Yunlian YHL) are two species of Coptidis Rhizoma commonly used in Traditional Chinese Medicine. Huanglian is produced from Sichuan Province, while Yunlian comes from the Yunnan Province. Both provinces are located in China. There are indications that both herbs possess similar characteristics and functions in clinical use. However, there are no signs of prior research to compare the effects of HL and YHL on hepatocellular carcinoma. In this study, we compared the components and anti-liver cancer effects in vitro between Huanglian and Yunlian. Methods: HPLC was introduced to analyze the active components in Huanglian and Yunlian aqueous extracts. Liquid chromatography with mass spectrometry and High performance liquid chromatography with UV detector were used to identify and compare the main ingredients ratio between Huanglian and Yunlian extracts. Cytotoxicity and apoptosis affected by the two extracts on MHCC97L cell line was observed by using MTT assay and flow cytometry, respectively. The inhibition of metastasis and invasion in hepatocellular carcinoma cell line MHCC97L by Huanglian and Yunlian were compared as well. Results: Results of phytochemical analysis showed there were no significant differences between the two herbs' ingredients and ratios. Also, there were no significant differences between Huanglian and Yunlian on cytotoxicity, apoptosis and metastasis in MHCC97L. Conclusion: Yunlian and Huanglian share great similarities in both chemical compostion and bioactivity in vitro. © 2012 To C, et al.published_or_final_versio

An ECG-SoC with 535nW/channel lossless data compression for wearable sensors

10.1109/ASSCC.2013.6691003Proceedings of the 2013 IEEE Asian Solid-State Circuits Conference, A-SSCC 2013145-14

An ECG-on-Chip with 535-nW/Channel Integrated Lossless Data Compressor for Wireless Sensors

This paper presents a low-power ECG recording system-on-chip (SoC) with on-chip low-complexity lossless ECG compression for data reduction in wireless/ambulatory ECG sensor devices. The chip uses a linear slope predictor for data compression, and incorporates a novel low-complexity dynamic coding-packaging scheme to frame the prediction error into fixed-length 16-bit format. The proposed technique achieves an average compression ratio of 2.25x on MIT/BIH ECG database. Implemented in a standard 0.35 um process, the compressor uses 0.565K gates/channel occupying 0.4 mm2 for four channels, and consumes 535 nW/channel at 2.4 V for ECG sampled at 512 Hz. Small size and ultra-low power consumption makes the proposed technique suitable for wearable ECG sensor applications

Patterns of medication use and factors associated with antibiotic use among adult fever patients at Singapore primary care clinics.

BACKGROUND: Antimicrobial resistance is a public health problem of global importance. In Singapore, much focus has been given to antibiotic usage patterns in hospital settings. Data on antibiotic use in primary care is lacking. We describe antibiotic usage patterns and assess factors contributing to antibiotic usage among adults presenting with acute febrile illness (AFI) in primary care settings in Singapore. METHODS: We analyzed data from the Early Dengue infection and outcome study. Adults with AFI presenting at 5 Singapore polyclinics were included. We used multivariable logistic regression to assess demographic, clinical and laboratory factors associated with antibiotic usage among adults with AFI. RESULTS: Between December 2007 and February 2013, 1884 adult AFI patients were enrolled. Overall, 16% of adult AFI patients reported antibiotic use. We observed a rise in the use of over-the-counter medications in late 2009 and a decrease in antibiotic use during 2010, possibly related to the outbreak of pandemic influenza A H1N1 virus. After adjusting for age, gender, polyclinic and year of enrolment, the following factors were associated with higher odds of antibiotic use: living in landed property (compared to public housing) (OR = 1.73; 95% CI: 1.06-2.80); body mass index (BMI) <18.5 (OR = 1.87; 95% CI: 1.19-2.93); elevated white blood cell (WBC) count (OR = 1.98; 95% CI: 1.42-2.78); and persistence of initial symptoms at 2-3 days follow-up with OR (95% CI) for categories of 1, 2, 3, and ≥4 persisting symptoms being 2.00 (1.38-2.92), 2.67 (1.80-3.97), 4.26 (2.73-6.64), and 2.79 (1.84-4.24) respectively. CONCLUSIONS: Our study provides insights on antibiotic usage among adult patients presenting to primary care clinics with febrile illness, and suggests that high socio-economic status, and risk factors of a severe illness, that is, low BMI and persistence of initial symptoms, are associated with higher antibiotic use. Further work to understand trends of antibiotic usage in both private and public primary care clinics, and factors that influence patient expectation and physician prescribing of antibiotics is important

3D Brain Segmentation Using Dual-Front Active Contours with Optional User Interaction

Important attributes of 3D brain cortex segmentation algorithms include robustness, accuracy, computational efficiency, and facilitation of user interaction, yet few algorithms incorporate all of these traits. Manual segmentation is highly accurate but tedious and laborious. Most automatic techniques, while less demanding on the user, are much less accurate. It would be useful to employ a fast automatic segmentation procedure to do most of the work but still allow an expert user to interactively guide the segmentation to ensure an accurate final result. We propose a novel 3D brain cortex segmentation procedure utilizing dual-front active contours which minimize image-based energies in a manner that yields flexibly global minimizers based on active regions. Region-based information and boundary-based information may be combined flexibly in the evolution potentials for accurate segmentation results. The resulting scheme is not only more robust but much faster and allows the user to guide the final segmentation through simple mouse clicks which add extra seed points. Due to the flexibly global nature of the dual-front evolution model, single mouse clicks yield corrections to the segmentation that extend far beyond their initial locations, thus minimizing the user effort. Results on 15 simulated and 20 real 3D brain images demonstrate the robustness, accuracy, and speed of our scheme compared with other methods

Optical source of individual pairs of colour-conjugated photons

We theoretically demonstrate that Kerr nonlinearity in optical circuits can lead to both resonant four-wave mixing and photon blockade, which can be used for high-yield generation of high-fidelity individual photon pairs with conjugated frequencies. We propose an optical circuit, which, in the optimal pulsed-drive regime, would produce photon pairs at the rate up to 5 × 105  s−1 (0.5 pairs per pulse) with g(2)(0)<10–2g(2)(0)<10−2 for one of the conjugated frequencies. We show that such a scheme can be utilised to generate colour-entangled photons

Chromosomal-level assembly of the Asian Seabass genome using long sequence reads and multi-layered scaffolding

We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. The population structure of L. calcarifer species complex was analyzed by re-sequencing 61 individuals representing various regions across the species' native range. SNP analyses identified high levels of genetic diversity and confirmed earlier indications of a population stratification comprising three clades with signs of admixture apparent in the South-East Asian population. The quality of the Asian seabass genome assembly far exceeds that of any other fish species, and will serve as a new standard for fish genomics