13 research outputs found
Taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism
Get PDFObjective: To evaluate the taxonomic composition of the gut microbiome in gout patients with and without tophi
formation, and predict bacterial functions that might have an impact on urate metabolism.
Methods: Hypervariable V3–V4 regions of the bacterial 16S rRNA gene from fecal samples of gout patients with
and without tophi (n=33 and n=25, respectively) were sequenced and compared to fecal samples from 53 healthy
controls. We explored predictive functional profles using bioinformatics in order to identify diferences in taxonomy
and metabolic pathways.
Results: We identifed a microbiome characterized by the lowest richness and a higher abundance of Phascolarctobacterium, Bacteroides, Akkermansia, and Ruminococcus_gnavus_group genera in patients with gout without tophi
when compared to controls. The Proteobacteria phylum and the Escherichia-Shigella genus were more abundant
in patients with tophaceous gout than in controls. Fold change analysis detected nine genera enriched in healthy
controls compared to gout groups (Bifdobacterium, Butyricicoccus, Oscillobacter, Ruminococcaceae_UCG_010, Lachnospiraceae_ND2007_group, Haemophilus, Ruminococcus_1, Clostridium_sensu_stricto_1, and Ruminococcaceae_
UGC_013). We found that the core microbiota of both gout groups shared Bacteroides caccae, Bacteroides stercoris ATCC
43183, and Bacteroides coprocola DSM 17136. These bacteria might perform functions linked to one-carbon metabo‑
lism, nucleotide binding, amino acid biosynthesis, and purine biosynthesis. Finally, we observed diferences in key
bacterial enzymes involved in urate synthesis, degradation, and elimination.
Conclusion: Our fndings revealed that taxonomic variations in the gut microbiome of gout patients with and with‑
out tophi might have a functional impact on urate metabolism.
Keywords: Gout, Gut microbiota, Uric acid metabolis
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No full textEl presente volumen reúne 21 ponencias de las 25 que se presentaron en el “Primer simposium sobre historia, sociedad y cultura de México y América Latina”, realizado el 27 y el 28 de octubre de 2005, en el Centro de Investigación en Ciencias Sociales y Humanidades (CICSyH) de la Universidad Autónoma del Estado de México (UAEM), en Toluca, Estado de México
Ameliorative effects of ark clams (Scapharca subcrenata and Tegillarca granosa) on endothelial dysfunction induced by a high-fat diet
No full textMemoria del primer simposium sobre historia, sociedad y cultura de México y América Latina
No full textEl presente volumen reúne 21 ponencias de las 25 que se presentaron en el “Primer simposium sobre historia, sociedad y cultura de México y América Latina”, realizado el 27 y el 28 de octubre de 2005, en el Centro de Investigación en Ciencias Sociales y Humanidades (CICSyH) de la Universidad Autónoma del Estado de México (UAEM), en Toluca, Estado de México
Effect of cadmium on the concentration of essential metals in a human chondrocyte micromass culture
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