34 research outputs found
Characterization of the Antiglioma Effect of the Oncolytic Adenovirus VCN-01
Despite the recent advances in the development of antitumor therapies, the prognosis for patients with malignant gliomas remains dismal. Therapy with tumor-selective viruses is emerging as a treatment option for this devastating disease. In this study we characterize the anti-glioma effect of VCN-01, an improved hyaluronidase-armed pRB-pathway-selective oncolytic adenovirus that has proven safe and effective in the treatment of several solid tumors. VCN-01 displayed a significant cytotoxic effect on glioma cells in vitro. In vivo, in two different orthotopic glioma models, a single intra-tumoral administration of VCN-01 increased overall survival significantly and led to long-term survivors free of disease
Development of a DIPG Orthotopic Model in Mice Using an Implantable Guide-Screw System
Objective
In this work we set to develop and to validate a new in vivo frameless orthotopic Diffuse Intrinsic Pontine Glioma (DIPG) model based in the implantation of a guide-screw system.
Methods
It consisted of a guide-screw also called bolt, a Hamilton syringe with a 26-gauge needle and an insulin-like 15-gauge needle. The guide screw is 2.6 mm in length and harbors a 0.5 mm central hole which accepts the needle of the Hamilton syringe avoiding a theoretical displacement during insertion. The guide-screw is fixed on the mouse skull according to the coordinates: 1mm right to and 0.8 mm posterior to lambda. To reach the pons the Hamilton syringe is adjusted to a 6.5 mm depth using a cuff that serves as a stopper. This system allows delivering not only cells but also any kind of intratumoral chemotherapy, antibodies or gene/viral therapies.
Results
The guide-screw was successfully implanted in 10 immunodeficient mice and the animals were inoculated with DIPG human cell lines during the same anesthetic period. All the mice developed severe neurologic symptoms and had a median overall survival of 95 days ranging the time of death from 81 to 116 days. Histopathological analysis confirmed tumor into the pons in all animals confirming the validity of this model.
Conclusion
Here we presented a reproducible and frameless DIPG model that allows for rapid evaluation of tumorigenicity and efficacy of chemotherapeutic or gene therapy products delivered intratumorally to the pons
Jardins per a la salut
Facultat de FarmĂ cia, Universitat de Barcelona. Ensenyament: Grau de FarmĂ cia. Assignatura: BotĂ nica farmacĂšutica. Curs: 2014-2015. Coordinadors: Joan Simon, CĂšsar BlanchĂ© i Maria Bosch.Els materials que aquĂ es presenten sĂłn el recull de les fitxes botĂ niques de 128 espĂšcies presents en el JardĂ Ferran Soldevila de lâEdifici HistĂČric de la UB. Els treballs han estat realitzats manera individual per part dels estudiants dels grups M-3 i T-1 de lâassignatura BotĂ nica FarmacĂšutica durant els mesos de febrer a maig del curs 2014-15 com a resultat final del Projecte dâInnovaciĂł Docent «Jardins per a la salut: aprenentatge servei a BotĂ nica farmacĂšutica» (codi 2014PID-UB/054). Tots els treballs sâhan dut a terme a travĂ©s de la plataforma de GoogleDocs i han estat tutoritzats pels professors de lâassignatura. Lâobjectiu principal de lâactivitat ha estat fomentar lâaprenentatge autĂČnom i col·laboratiu en BotĂ nica farmacĂšutica. TambĂ© sâha pretĂšs motivar els estudiants a travĂ©s del retorn de part del seu esforç a la societat a travĂ©s dâuna experiĂšncia dâAprenentatge-Servei, deixant disponible finalment el treball dels estudiants per a poder ser consultable a travĂ©s dâuna Web pĂșblica amb la possibilitat de poder-ho fer in-situ en el propi jardĂ mitjançant codis QR amb un smartphone
Correction: Characterization of the Antiglioma Effect of the Oncolytic Adenovirus VCN-01.
[This corrects the article DOI: 10.1371/journal.pone.0147211.]
CBIO-03ALTERNATIVE SPLICING IN GLIOBLASTOMA MULTIFORME: CHARACTERIZATION OF Baf45d ABERRANT SPLICING IN THE PATHOGENESIS OF GLIOBLASTOMA
Characterization of the Antiglioma Effect of the Oncolytic Adenovirus VCN-01.
Despite the recent advances in the development of antitumor therapies, the prognosis for patients with malignant gliomas remains dismal. Therapy with tumor-selective viruses is emerging as a treatment option for this devastating disease. In this study we characterize the anti-glioma effect of VCN-01, an improved hyaluronidase-armed pRB-pathway-selective oncolytic adenovirus that has proven safe and effective in the treatment of several solid tumors. VCN-01 displayed a significant cytotoxic effect on glioma cells in vitro. In vivo, in two different orthotopic glioma models, a single intra-tumoral administration of VCN-01 increased overall survival significantly and led to long-term survivors free of disease
Correction: Characterization of the Antiglioma Effect of the Oncolytic Adenovirus VCN-01
Characterization of the Antiglioma Effect of the Oncolytic Adenovirus VCN-01
Despite the recent advances in the development of antitumor therapies, the prognosis for patients with malignant gliomas remains dismal. Therapy with tumor-selective viruses is emerging as a treatment option for this devastating disease. In this study we characterize the anti-glioma effect of VCN-01, an improved hyaluronidase-armed pRB-pathway-selective oncolytic adenovirus that has proven safe and effective in the treatment of several solid tumors. VCN-01 displayed a significant cytotoxic effect on glioma cells in vitro. In vivo, in two different orthotopic glioma models, a single intra-tumoral administration of VCN-01 increased overall survival significantly and led to long-term survivors free of disease
The influence of Paul Otlet upon the World Wide Web
W projektach Paula Otleta daje siÄ wskazaÄ podobieĆstwa do systemu
hipertekstowego World Wide Web. Badacze wywodzÄ
cy siÄ z rĂłĆŒnych Ćrodowisk
(m.in. medioznawczych, socjologicznych, informatycznych, bibliologiczno-informatologicznych)
zwracajÄ
uwagÄ na podobieĆstwa pomiÄdzy projektami systemu informacyjno-
wyszukiwawczego Otleta a WWW. Celem artykuĆu jest prĂłba odpowiedzi
na pytania, czy projekty Otleta sÄ
zbieĆŒne pod pewnymi wzglÄdami z WWW oraz
czy tworzÄ
c Web, wzorowano siÄ na pracach Otleta.Paul Otletâs visualizations of data integration seem to have some distinct
parallels with the hypertext used in the World Wide Web. Researchers representing
different disciplines (e.g., media specialists, sociologists, IT specialists and librarians) all indicate essential similarities between the attempts at the information and search
system conceived by the Belgian author and visionary and that of the WWW. Accordingly,
the article aims at finding an answer to the question whether Otletâs visualizations
are convergent to a certain degree with the WWW and whether the authors of
the Web modeled their work on the Otletâs achievements?171531678Bibliotek