Estimation of pedigree errors in the UK dairy population using microsatellite markers and the impact on selection

The proportion of cows in the UK dairy herd whose sires were misidentified was estimated using DNA markers. Genetic marker genotypes were determined on 568 cows (from 168 milk samples and 400 hair samples) and 96 putative sires (from semen samples). The estimated pedigree error rate from the hair samples was 8.8%, and from the milk samples, 13.1%, giving an overall estimate of the error rate of 10%. This level of pedigree errors will have a relatively large impact on the efficiency of progeny testing and the accuracy of cow predicted breeding values. We predict a loss of response to selection of approximately 2 to 3% given this error rate

Fabrication of high quality optical coherence tomography (OCT) calibration artefacts using femtosecond inscription

Optical coherence tomography (OCT) is a non-invasive three-dimensional imaging system that is capable of producing high resolution in-vivo images. OCT is approved for use in clinical trials in Japan, USA and Europe. For OCT to be used effectively in a clinical diagnosis, a method of standardisation is required to assess the performance across different systems. This standardisation can be implemented using highly accurate and reproducible artefacts for calibration at both installation and throughout the lifetime of a system. Femtosecond lasers can write highly reproducible and highly localised micro-structured calibration artefacts within a transparent media. We report on the fabrication of high quality OCT calibration artefacts in fused silica using a femtosecond laser. The calibration artefacts were written in fused silica due to its high purity and ability to withstand high energy femtosecond pulses. An Amplitude Systemes s-Pulse Yb:YAG femtosecond laser with an operating wavelength of 1026 nm was used to inscribe three dimensional patterns within the highly optically transmissive substrate. Four unique artefacts have been designed to measure a wide variety of parameters, including the points spread function (PSF), modulation transfer function (MTF), sensitivity, distortion and resolution - key parameters which define the performance of the OCT. The calibration artefacts have been characterised using an optical microscope and tested on a swept source OCT. The results demonstrate that the femtosecond laser inscribed artefacts have the potential of quantitatively and qualitatively validating the performance of any OCT system

Parametric study of femtosecond inscription of microstructures for OCT artefact fabrication

As optical coherence tomography (OCT) becomes widespread, validation and characterization of systems becomes important. Reference standards are required to qualitatively and quantitatively measure the performance between difference systems. This would allow the performance degradation of the system over time to be monitored. In this report, the properties of the femtosecond inscribed structures from three different systems for making suitable OCT characterization artefacts (phantoms) are analyzed. The parameter test samples are directly inscribed inside transparent materials. The structures are characterized using an optical microscope and a swept-source OCT. The high reproducibility of the inscribed structures shows high potential for producing multi-modality OCT calibration and characterization phantoms. Such that a single artefact can be used to characterize multiple performance parameters such the resolution, linearity, distortion, and imaging depths

Quantitative optical coherence tomography for characterization of microscopic structures with varying refractive index

In this paper we have done back to back comparison of quantitive phase and refractive index from a microscopic image of waveguide previously obtained by Allsop et al. Paper also shows microscopic image of the first 3 waveguides from the sample. Tomlins et al. have demonstrated use of femtosecond fabricated artefacts as OCT calibration samples. Here we present the use of femtosecond waveguides, inscribed with optimized parameters, to test and calibrate the sensitivity of the OCT systems

Design and characterisation of an additive manufacturing benchmarking artefact following a design-for-metrology approach

We present the design and characterisation of a high-speed sintering additive manufacturing benchmarking artefact following a design-for-metrology approach. In an important improvement over conventional approaches, the specifications and operating principles of the instruments that would be used to measure the manufactured artefact were taken into account during its design process. With the design-for-metrology methodology, we aim to improve and facilitate measurements on parts produced using additive manufacturing. The benchmarking artefact has a number of geometrical features, including sphericity, cylindricity, coaxiality and minimum feature size, all of which are measured using contact, optical and X-ray computed tomography coordinate measuring systems. The results highlight the differences between the measuring methods, and the need to establish a specification standards and guidance for the dimensional assessment of additive manufacturing parts

Applying metrological techniques to satellite fundamental climate data records

Quantifying long-term environmental variability, including climatic trends, requires decadal-scale time series of observations. The reliability of such trend analysis depends on the long-term stability of the data record, and understanding the sources of uncertainty in historic, current and future sensors. We give a brief overview on how metrological techniques can be applied to historical satellite data sets. In particular we discuss the implications of error correlation at different spatial and temporal scales and the forms of such correlation and consider how uncertainty is propagated with partial correlation. We give a form of the Law of Propagation of Uncertainties that considers the propagation of uncertainties associated with common errors to give the covariance associated with Earth observations in different spectral channels

Three-dimensional calibration targets for optical coherence tomography

The recent expansion of clinical applications for optical coherence tomography (OCT) is driving the development of approaches for consistent image acquisition. There is a simultaneous need for time-stable, easy-to-use imaging targets for calibration and standardization of OCT devices. We present calibration targets consisting of three-dimensional structures etched into nanoparticle-embedded resin. Spherical iron oxide nanoparticles with a predominant particle diameter of 400 nm were homogeneously dispersed in a two part polyurethane resin and allowed to harden overnight. These samples were then etched using a precision micromachining femtosecond laser with a center wavelength of 1026 nm, 100kHz repetition rate and 450 fs pulse duration. A series of lines in depth were etched, varying the percentage of inscription energy and speed of the translation stage moving the target with respect to the laser. Samples were imaged with a dual wavelength spectral-domain OCT system and point-spread function of nanoparticles within the target was measured

An interlaboratory comparison of X-ray computed tomography measurement for texture and dimensional characterisation of additively manufactured parts

Abstract This paper presents the results of the CT-STARR (CT-Surface Texture for Additive Round Robin) Stage 1 interlaboratory comparison. The study compared the results obtained for the extraction of areal surface texture data per ISO 25178-2 from five X-ray computed tomography (CT) volume measurements from each of four laboratories. Two Ti6Al4V ELI (extra-low interstitial) components were included in each of the CT acquisitions. The first component was an additively manufactured (AM) cube manufactured using an Arcam Q10 electron beam melting (EBM) machine. Surface texture data was extracted from CT scans of this part. The values of selected parameters per ISO 25178-2 are reported, including Sa, the arithmetic mean height, for which the values from the Nikon MCT 225 metrology CT measurements were all within 0.5% of the mean reference focus variation measurement. CT resolution requirements are discussed. The second component was a machined dimensional test artefact designed to facilitate independent analysis of CT global voxel scaling errors and surface determination errors. The results of mathematical global scaling and surface determination correction of the dimensional artefact data is reported. The dimensional test artefact errors for the XT H 225 commercial CT for length, outside diameter and inside diameter reduced from -0.27%, -0.83% and -0.54% respectively to less than 0.02% after performing mathematical correction. This work will assist the development of surface texture correction protocols, help define surface-from-CT measurement envelope limits and provide valuable information for an expanded Stage 2 interlaboratory comparison, which will include a more diverse range of CT systems and technologies, further expanding the surface-from-CT knowledge base

Siyasi komiserler yeni başbakanı ayaklarına kadar getirttiler:Ali Rıza Paşa İngiliz yardımını talep etti

Taha Toros Arşivi, Dosya Adı: Milli Mücadele İstiklal Harbi GazetesiUnutma İstanbul projesi İstanbul Kalkınma Ajansı'nın 2016 yılı "Yenilikçi ve Yaratıcı İstanbul Mali Destek Programı" kapsamında desteklenmiştir. Proje No: TR10/16/YNY/010

A novel framework to harmonise satellite data series for climate applications

Fundamental and thematic climate data records derived from satellite observations provide unique information for climate monitoring and research. Since any satellite operates over a limited period of time only, creating a climate data record requires the combination of space-borne measurements from a series of several (often similar) satellite sensors. A simple combination of calibrated measurements from several sensors, however, can produce an inconsistent climate data record. This is particularly true of older, historic sensors whose behavior in space was often different from their behavior during pre-launch calibration in the laboratory. More scientific value can be derived from considering the series of historical and present satellites as a whole. Here we consider harmonisation as a process that obtains new calibration coefficients for revised sensor calibration models by comparing calibrated measurements over appropriate satellite-to-satellite match-ups, such as simultaneous nadir overpasses. When we perform a comparison of two sensors, however, we must consider that those sensors are not observing exactly the same Earth radiance. This is in part due to differences in exact location and time tolerated by the match-up process itself, but also due to differences in the spectral response functions of the two instruments, even when nominally observing the same spectral band. To derive a harmonised data set we do not aim to correct for spectral response function differences, but to reconcile the calibration of different sensors given their estimated spectral response function differences. Here we present the concept of a framework that establishes calibration coefficients and their uncertainty and error covariance for an arbitrary number of sensors in a metrologically-rigorous manner. We describe harmonisation and its mathematical formulation as an inverse problem. Solving this problem is challenging when some hundreds of millions of match-ups are involved and the errors of fundamental sensor measurements are correlated. We solve the harmonisation problem as marginalised errors in variables regression. The algorithm involves computation of first and second order partial derivatives, for which the corresponding computer source code is generated by Automatic Differentiation. Finally we present re-calibrated AVHRR radiances from a series of 10 sensors. It is shown that the new time series have much less match-up differences while being consistent with uncertainty statistics