«El museo de arte en el curriculum escolar».

Titis article analyzes the history and use of the art museum as a publicfacility and as att educational tool. implementation of tite art museum into middle school curriculum has proved beneficial, ifnot necessary, for a child's aesthetic aná cultural appreciation of the world. PALABRAS CL4 VE: estética-estético, cultural literacy, currículum, implementación, maluca/sural.Este artículo analiza la historia y empleo del museo de arte como espacio público y como herramienta educativa. La puesta en práctica del museo de arte dentm del currículum del primer periodo de la enseñanza secundaria ha demostrado ser beneficiosa, cuando no necesaria, para la apreciación estética y cultural del mundo por parte del niño

«El museo de arte en el curriculum escolar».

Titis article analyzes the history and use of the art museum as a publicfacility and as att educational tool. implementation of tite art museum into middle school curriculum has proved beneficial, ifnot necessary, for a child's aesthetic aná cultural appreciation of the world. PALABRAS CL4 VE: estética-estético, cultural literacy, currículum, implementación, maluca/sural

Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides

Previously we have shown that the presence of 3-nitrotyrosine within a peptide sequence severely depletes the peptide backbone fragments typically observed following electron capture dissociation (ECD) mass spectrometry. Instead, ECD of nitrated peptides is characterised by abundant losses of small neutrals (hydroxyl radicals, water and ammonia). Here, we investigate the origin of ammonia loss by comparing the ECD behaviour of lysine- and arginine-containing nitrated peptides, and their N-acetylated counterparts, and nitrated peptides containing no basic amino acid residues. The results reveal that ammonia loss derives from the N-terminus of the peptides, however, the key finding of this work is the insight provided into the hierarchy of various proposed ECD mechanisms: the Utah-Washington mechanism, the electron predator mechanism and the Oslo mechanism

Activated Ion Electron Capture Dissociation (AI ECD) of proteins: synchronization of infrared and electron irradiation with ion magnetron motion.

Here, we show that to perform activated ion electron capture dissociation (AI-ECD) in a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer equipped with a CO(2) laser, it is necessary to synchronize both infrared irradiation and electron capture dissociation with ion magnetron motion. This requirement is essential for instruments in which the infrared laser is angled off-axis, such as the Thermo Finnigan LTQ FT. Generally, the electron irradiation time required for proteins is much shorter (ms) than that required for peptides (tens of ms), and the modulation of ECD, AI ECD, and infrared multiphoton dissociation (IRMPD) with ion magnetron motion is more pronounced. We have optimized AI ECD for ubiquitin, cytochrome c, and myoglobin; however the results can be extended to other proteins. We demonstrate that pre-ECD and post-ECD activation are physically different and display different kinetics. We also demonstrate how, by use of appropriate AI ECD time sequences and normalization, the kinetics of protein gas-phase refolding can be deconvoluted from the diffusion of the ion cloud and measured on the time scale longer than the period of ion magnetron motion

El museo de arte en el currículum escolar

Este artículo analiza la historia y empleo del museo de arte como espacio público y como herramienta educativa. La puesta en práctica del museo de arte dentro del currículum del primer periodo de la enseñanza secundaria ha demostrado ser beneficiosa, cuando no necesaria, para la operación estética y cultural del mundo por parte del niñ