A selectable-bandwidth 3.5 mW, 0.03 mm(2) self-oscillating Sigma Delta modulator with 71 dB dynamic range at 5 MHz and 65 dB at 10 MHz bandwidth

In this paper we present a dual-mode third order continuous time Sigma Delta modulator that combines noise shaping and pulse-width-modulation (PWM). In our 0.18 micro-m CMOS prototype chip the clock frequency equals 1 GHz, but the PWM carrier is only around 125 MHz. By adjusting the loop filter, the ADC bandwidth can be set to 5 or 10 MHz. In the 5 MHz mode the peak SNDR equals 64 dB and the dynamic range 71 dB. In the 10 MHz mode the peak SNDR equals 58 dB and the DR 65 dB. This performance is achieved at an attractively low silicon area of 0.03 mm^2 and a power consumption of 3.5 mW

A dual-mass capacitive-readout accelerometer operated near pull-in

A mechanical two-mass configuration and a readout circuit for a single-axis capacitive-readout accelerometer with ΣΔ force-feedback is presented. The system reduces electrical and quantisation input-referred noise through the use of negative springs, reduced gaps in the readout capacitors and maximised readout voltage. A theoretical analysis and simulation results are discussed

A very compact 1MS/s Nyquist-rate A/D-converter with 12 effective bits

We present a very compact analog-to-digital convertor (ADC) for use as a standard cell. To achieve an inherent accuracy of at least 12-bits without trimming or calibration, extended counting A/D-conversion is used. Here, the circuit performs a conversion by passing through two modes of operation: first it works as a 1st-order incremental convertor and then it is reconfigured to operate as a conventional algorithmic converter. This way, we obtain a Nyquist-rate converter that requires only 1 operational amplifier and achieves 12-bit accuracy performance in 13 clock cycles with 9 bit capacitor matching. The circuit is designed in 0.18 mu m CMOS with a thick oxide option. The resulting analog core occupies a chip area of only 0.011 mm2 and the complete digital control and reconstruction logic (including additional test features and storage registers) is 0.02 mm2. The analog blocks of the circuit consume 1.2mW and the digital 0.4mW. At a sample rate of 1 MS/s, the peak SNDR is 74.5dB and the dynamic range is 78dB, constant over the Nyquist band. The worst-case integral non-lineairity (INL) is within plus or minus 0.55 LSB

Robot competitions trick students into learning

It has been shown in the past that robots help to bring theoretical concepts into practice, while at the same time increasing the motivation of the students. Despite these benefits, robots are hardly ever integrated in education programs and at the same time students feel that they have the competences nor the infrastructure to build a robot on their own. Therefore the workgroup electronics (WELEK) of Ghent University gives students the opportunity to build a robot by organizing workshops and competitions. Up until now, four competitions were organized in which over 200 students voluntarily participated. This paper describes our approach in the hope that it will inspire other educators to do the same thing. We also measured the effectiveness of our competitions by sending each of the participants a questionnaire. The results confirm that students acquire relevant technical competences by building a robot, learn to work as a team and are challenged to use their creativity

Crystal structure of Spa40, the specificity switch for the Shigella flexneri type III secretion system

The pathogenic bacterium Shigella flexneri uses a type III secretion system to inject virulence factors from the bacterial cytosol directly into host cells. The machinery that identifies secretion substrates and controls the export of extracellular components and effector proteins consists of several inner-membrane and cytoplasmic proteins. One of the inner membrane components, Spa40, belongs to a family of proteins proposed to regulate the switching of substrate specificity of the export apparatus. We show that Spa40 is cleaved within the strictly conserved amino acid sequence NPTH and substitution of the proposed autocatalytic residue abolishes cleavage. Here we also report the crystal structure of the cytoplasmic complex Spa40C and compare it with the recent structures of the homologues from Escherichia coli and Salmonella typhimurium. These structures reveal the tight association of the cleaved fragments and show that the conserved NPTH sequence lies on a loop which, when cleaved, swings away from the catalytic N257 residue, resulting in different surface features in this region. This structural rearrangement suggests a mechanism by which non-cleaving forms of these proteins interfere with correct substrate switching of the apparatus

Structure of the type III secretion and substrate-binding domain of Yersinia YopH phosphatase

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72457/1/j.0950-382x.2001.02711.x.pd

Characterization and intracellular localization of putative Chlamydia pneumoniae effector proteins

We here describe four proteins of Chlamydia pneumoniae, which might play a role in host-pathogen interaction. The hypothetical bacterial proteins CPn0708 and CPn0712 were detected in Chlamydia pneumoniae-infected host cells by indirect immunofluorescence tests with polyclonal antisera raised against the respective proteins. While CPn0708 was localized within the inclusion body, CPn0712 was identified in the inclusion membrane and in the surrounding host cell cytosol. CPn0712 colocalizes with actin, indicating its possible interaction with components of the cytoskeleton. Investigations on CPn0809 and CPn1020, two Chlamydia pneumoniae proteins previously described to be secreted into the host cell cytosol, revealed colocalization with calnexin, a marker for the ER. Neither CPn0712, CPn0809 nor CPn1020 were able to inhibit host cell apoptosis. Furthermore, transient expression of CPn0712, CPn0809 and CPn1020 by the host cell itself had no effect on subsequent infection with Chlamydia pneumoniae. However, microarray analysis of CPn0712-expressing host cells revealed six host cell genes which were regulated as in host cells infected with Chlamydia pneumoniae, indicating the principal usefulness of heterologous expression to study the effect of Chlamydia pneumoniae proteins on host cell modulation

Differential Effects of Bartonella henselae on Human and Feline Macro- and Micro-Vascular Endothelial Cells

Bartonella henselae, a zoonotic agent, induces tumors of endothelial cells (ECs), namely bacillary angiomatosis and peliosis in immunosuppressed humans but not in cats. In vitro studies on ECs represent to date the only way to explore the interactions between Bartonella henselae and vascular endothelium. However, no comparative study of the interactions between Bartonella henselae and human (incidental host) ECs vs feline (reservoir host) ECs has been carried out because of the absence of any available feline endothelial cell lines