Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem BlutDFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und KonsequenzenEC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und PlastizitätDFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory syste

Design Goals for Consent at Scale in Digital Service Ecosystems

Digital services have undergone a shift to multi-actor constellations characterised by the utilisation of personal data. By involving external actors, service capability and variety increase but so does the number of actors that gain access to personal data. Here, privacy policies are legal documents that serve two primary functions: specifying the purpose and details of data processing in a binding manner and informing users about it. Privacy policiestherefore have special importance in which the processing is based on user consent. In a case study of the platform eBay, we identified 18 problems that point out difficulties in achieving consent in a meaningful way in today’s large-scale and massively interconnected digital service ecosystems. Based on these problems, the design goals are determined which help to find meaningful consent in digital service ecosystems. These goals include notifications for changed purposes of data processing in ecosystems or the reasonability of time needed for consent in relation to the usage time of the service. Thus far, no legal limits govern the reasonability of efforts for consent to privacy policies. This requires a fundamental rethinking of the concept of consent or far-reaching automation of privacy-related legal acts

The Unlikely Siblings in the GDPR Family: A Techno-Legal Analysis of Major Platforms in the Diffusion of Personal Data in Service Ecosystems

The digital age is characterized by hyper-connected services. Whenever we engage with an app we likely engage with a broader set of actors, often facilitated by a platform. Essentially, we engage with a service ecosystem posing particular challenges for privacy regulation. With GDPR taking effect we seek to understand the implications of it for privacy in such ecosystems. Interconnected services can facilitate the diffusion of personal data and thus impede with individual privacy rights. We apply a novel techno- legal analysis to the flow of personal information in service ecosystems. Based on two cases, we show that novel requirements arise for platforms as key actors in service ecosystems. Using our techno-legal analysis we conclude that two major platform providers, Apple and Facebook, have more in common from a legal perspective than the current rhetoric suggests. Based on the analysis, we discuss where privacy-preserving solutions in service ecosystems need to be positioned

License file LICENSE

Title Rank Product method for identifying differentially expresse

CABLES1 Deficiency Impairs Quiescence and Stress Responses of Hematopoietic Stem Cells in Intrinsic and Extrinsic Manners

International audienc

In vitro and in vivo efficacy of an anti-CD203c conjugated antibody (AGS-16C3F) in mouse models of advanced systemic mastocytosis

International audienc

In-depth virological and immunological characterization of HIV-1 cure after CCR5 Delta 32/Delta 32 allogeneic hematopoietic stem cell transplantation

Despite scientific evidence originating from two patients published to date that CCR5 Delta 32/Delta 32 hematopoietic stem cell transplantation (HSCT) can cure human immunodeficiency virus type 1 (HIV-1), the knowledge of immunological and virological correlates of cure is limited. Here we characterize a case of long-term HIV-1 remission of a 53-year-old male who was carefully monitored for more than 9 years after allogeneic CCR5 Delta 32/Delta 32 HSCT performed for acute myeloid leukemia. Despite sporadic traces of HIV-1 DNA detected by droplet digital PCR and in situ hybridization assays in peripheral T cell subsets and tissue-derived samples, repeated ex vivo quantitative and in vivo outgrowth assays in humanized mice did not reveal replication-competent virus. Low levels of immune activation and waning HIV-1-specific humoral and cellular immune responses indicated a lack of ongoing antigen production. Four years after analytical treatment interruption, the absence of a viral rebound and the lack of immunological correlates of HIV-1 antigen persistence are strong evidence for HIV-1 cure after CCR5 Delta 32/Delta 32 HSCT. The recipient of an allogeneic stem cell transplant from a CCR5 Delta 32/Delta 32 donor shows evidence of HIV type 1 cure, including the absence of a viral rebound over 4 years after stopping antiretroviral treatment