9 research outputs found
Efeito do ensacamento dos frutos no controle de pragas e doenças e na qualidade e maturação de maçãs âFuji Supremaâ.
O objetivo deste trabalho foi avaliar a eficiĂȘncia de embalagens de diferentes materiais para a proteção contra pragas e doenças e seu efeito sobre a qualidade fĂsico-quĂmica, maturação e teor de cĂĄlcio (Ca) em maçãs âFuji Supremaâ. O experimento foi desenvolvido nas safras de 2007/2008 e 2008/2009, em pomar manejado sob o sistema orgĂąnico, localizado na regiĂŁo de SĂŁo Joaquim (SC). O pomar era composto por plantas de dez anos de idade da cultivar Fuji Suprema, sobre porta-enxerto âMarubakaidoâ, com interenxerto âEM-9â. Depois do raleio manual, aproximadamente 40 dias apĂłs a plena floração, os frutos foram ensacados com embalagens plĂĄsticas transparentes microperfuradas ou de tecido nĂŁo texturizado (TNT). Os frutos foram mantidos ensacados atĂ© a colheita. A testemunha foi constituĂda por frutos nĂŁo ensacados. Na colheita, os frutos foram avaliados quanto aos danos provocados por mosca-das-frutas (Anastrepha fraterculus), mariposa oriental (Grapholita molesta) e lagarta enroladeira (Bonagota salubricola). As doenças foram avaliadas pela incidĂȘncia de sarna da macieira (Venturia inaequalis), podridĂŁo amarga (Colletotrichum gloeosporioides) e podridĂŁo carpelar (Alternaria sp., Fusarium sp.). TambĂ©m foi avaliada a incidĂȘncia de distĂșrbios fisiolĂłgicos ârussetingâ e âbitter pitâ, atributos fĂsico-quĂmicos de maturação e qualidade e o teor de Ca nos frutos. Independentemente do tipo de embalagem verificou-se que o ensacamento Ă© prĂĄtica eficaz na proteção contra o ataque de insetos, mas nĂŁo reduz a incidĂȘncia e o desenvolvimento de doenças nos frutos. Na safra de 2008/2009, o ensacamento dos frutos aumentou o teor de Ca e reduziu a incidĂȘncia de âbitter pitâ, e aumentou a incidĂȘncia do ârussetingâ. O ensacamento dos frutos antecipou a maturação, especialmente com embalagem plĂĄstica transparente microperfurada, e reduziu a coloração vermelha, especialmente com embalagem TNT
Molecular characterisation of a novel cyclase from plasmodium falciparum
SIGLEAvailable from British Library Document Supply Centre-DSC:DXN026519 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
Comparison of the C2A domain of synaptotagmin-I and annexin-V as probes for detecting cell death
Rapid imaging of tumor cell death <i>in vivo </i>using the c2a domain of synaptotagmin-I
Cell death is an important target for imaging the early response of tumors to treatment. We describe here the validation of a phosphatidylserine-binding agent for detecting tumor cell death in vivo based on the C2A domain of synaptotagmin-I. Methods: The capability of near-infrared fluorophore-labeled and 99mTc- and 111In-labeled derivatives of C2Am for imaging tumor cell death, using planar near-infrared fluorescence imaging and SPECT, respectively, was evaluated in implanted and genetically engineered mouse models of lymphoma and in a human colorectal xenograft. Results: The fluorophore-labeled C2Am derivative showed predominantly renal clearance and high specificity and sensitivity for detecting low levels of tumor cell death (2%â5%). There was a significant correlation (R > 0.9, P < 0.05) between fluorescently labeled C2Am binding and histologic markers of cell death, including cleaved caspase-3, whereas there was no such correlation with a site-directed mutant of C2Am (iC2Am) that does not bind phosphatidylserine. 99mTc-C2Am and 111In-C2Am also showed favorable biodistribution profiles, with predominantly renal clearance and low nonspecific retention in the liver and spleen at 24 h after probe administration. 99mTc-C2Am and 111In-C2Am generated tumor-to-muscle ratios in drug-treated tumors of 4.3Ă and 2.2Ă, respectively, at 2 h and 7.3Ă and 4.1Ă, respectively, at 24 h after administration. Conclusion: Given the favorable biodistribution profile of 99mTc- and 111In-labeled C2Am, and their ability to produce rapid and cell deathâspecific image contrast, these agents have potential for clinical translation
Genome sequencing and characterization of an extensively drug-resistant sequence type 111 serotype O12 hospital outbreak strain of Pseudomonas aeruginosa
Epinephrine Regulation of Amino Acid Transport in Rat Hepatocytes Isolated During Development
The Alpha Variant B.1.1.7 was not associated with excess healthcare acquired COVID-19 infection in a multi-centre UK hospital study
Genomic epidemiology of SARS-CoV-2 in a university outbreak setting and implications for public health planning
Whole genome sequencing of SARS-CoV-2 has occurred at an unprecedented scale, and can be exploited for characterising outbreak risks at the fine-scale needed to inform control strategies. One setting at continued risk of COVID-19 outbreaks are higher education institutions, associated with student movements at the start of term, close living conditions within residential halls, and high social contact rates. Here we analysed SARS-CoV-2 whole genome sequences in combination with epidemiological data to investigate a large cluster of student cases associated with University of Glasgow accommodation in autumn 2020, Scotland. We identified 519 student cases of SARS-CoV-2 infection associated with this large cluster through contact tracing data, with 30% sequencing coverage for further analysis. We estimated at least 11 independent introductions of SARS-CoV-2 into the student population, with four comprising the majority of detected cases and consistent with separate outbreaks. These four outbreaks were curtailed within a week following implementation of control measures. The impact of student infections on the local community was short-term despite an underlying increase in community infections. Our study highlights the need for context-specific information in the formation of public health policy for higher educational settings