Association of selected inflammatory markers and risk factors with pain in patients undergoing cervical or lumbar disc herniation

Introduction Studies have suggested that radicular pain following disc herniation may be associated with local or systemic inflammatory responses produced in the vicinity of the herniated disc rather than compression of the nerve. Present study assessed the association of serum interleukin-6 (IL-6) levels, high-sensitivity C-reactive protein (hs-CRP) and related risk factors in patients undergoing discectomy following cervical disc herniation (CDH) or lumbar disc herniation (LDH). Methods Study recruited 77 patients undergoing discectomy for LDH and CDH. An interviewer administered questionnaire was used to obtain sociodemographic, behavioral and occupational data. IL-6 and hs-CRP levels were measured in each individual. IL-6 was analyzed by ELISA method and hs-CRP was determined using auto analyzer. Results were analyzed by using SPSS version 25.0.   Results Significant correlation was not found with serum IL-6 and hs-CRP levels (r=0.02, p˃0.05) for acute and chronic CDH and LDH. Patients with chronic neck or back pain showed significantly (p=0.043) higher IL-6 level compared to patients with acute neck or back pain. Serum hs-CRP level was significantly higher (p=0.048) in patients with acute neck or back pain compared to patients with chronic neck or back pain. Significant association was not observed between; level of physically demanding nature of occupation (p=0.542), duration of occupation (p=0.446), type of exercise (p=0.371), type of sports (p=0.339) and CDH or LDH. Conclusion Though significant correlation was not observed between inflammatory markers IL-6, hs-CRP and CDH or LDH. The mean IL-6 was higher in chronic back pain and neck pain patients with LDH and CDH whereas mean hs-CRP mean was higher in acute back pain and neck pain patients. Significant association was not reported between selected known risk factors (behavioral, occupational) and CDH or LDH

Dietary Supplementation with Soluble Plantain Non-Starch Polysaccharides Inhibits Intestinal Invasion of Salmonella Typhimurium in the Chicken

Soluble fibres (non-starch polysaccharides, NSP) from edible plants but particularly plantain banana (Musa spp.), have been shown in vitro and ex vivo to prevent various enteric pathogens from adhering to, or translocating across, the human intestinal epithelium, a property that we have termed contrabiotic. Here we report that dietary plantain fibre prevents invasion of the chicken intestinal mucosa by Salmonella. In vivo experiments were performed with chicks fed from hatch on a pellet diet containing soluble plantain NSP (0 to 200 mg/d) and orally infected with S.Typhimurium 4/74 at 8 d of age. Birds were sacrificed 3, 6 and 10 d post-infection. Bacteria were enumerated from liver, spleen and caecal contents. In vitro studies were performed using chicken caecal crypts and porcine intestinal epithelial cells infected with Salmonella enterica serovars following pre-treatment separately with soluble plantain NSP and acidic or neutral polysaccharide fractions of plantain NSP, each compared with saline vehicle. Bacterial adherence and invasion were assessed by gentamicin protection assay. In vivo dietary supplementation with plantain NSP 50 mg/d reduced invasion by S.Typhimurium, as reflected by viable bacterial counts from splenic tissue, by 98.9% (95% CI, 98.1–99.7; P<0.0001). In vitro studies confirmed that plantain NSP (5–10 mg/ml) inhibited adhesion of S.Typhimurium 4/74 to a porcine epithelial cell-line (73% mean inhibition (95% CI, 64–81); P<0.001) and to primary chick caecal crypts (82% mean inhibition (95% CI, 75–90); P<0.001). Adherence inhibition was shown to be mediated via an effect on the epithelial cells and Ussing chamber experiments with ex-vivo human ileal mucosa showed that this effect was associated with increased short circuit current but no change in electrical resistance. The inhibitory activity of plantain NSP lay mainly within the acidic/pectic (homogalacturonan-rich) component. Supplementation of chick feed with plantain NSP was well tolerated and shows promise as a simple approach for reducing invasive salmonellosis

Gene Expression Profiles of Chicken Embryo Fibroblasts in Response to Salmonella Enteritidis Infection

The response of chicken to non-typhoidal Salmonella infection is becoming well characterised but the role of particular cell types in this response is still far from being understood. Therefore, in this study we characterised the response of chicken embryo fibroblasts (CEFs) to infection with two different S. Enteritidis strains by microarray analysis. The expression of chicken genes identified as significantly up- or down-regulated (≥3-fold) by microarray analysis was verified by real-time PCR followed by functional classification of the genes and prediction of interactions between the proteins using Gene Ontology and STRING Database. Finally the expression of the newly identified genes was tested in HD11 macrophages and in vivo in chickens. Altogether 19 genes were induced in CEFs after S. Enteritidis infection. Twelve of them were also induced in HD11 macrophages and thirteen in the caecum of orally infected chickens. The majority of these genes were assigned different functions in the immune response, however five of them (LOC101750351, K123, BU460569, MOBKL2C and G0S2) have not been associated with the response of chicken to Salmonella infection so far. K123 and G0S2 were the only 'non-immune' genes inducible by S. Enteritidis in fibroblasts, HD11 macrophages and in the caecum after oral infection. The function of K123 is unknown but G0S2 is involved in lipid metabolism and in β-oxidation of fatty acids in mitochondria

On the typology and the worship status of sacred trees with a special reference to the Middle East

This article contains the reasons for the establishment of sacred trees in Israel based on a field study. It includes 97 interviews with Muslim and Druze informants. While Muslims (Arabs and Bedouins) consider sacred trees especially as an abode of righteous figures' (Wellis') souls or as having a connection to their graves, the Druze relate sacred trees especially to the events or deeds in the lives of prophets and religious leaders. A literary review shows the existence of 24 known reasons for the establishment of sacred trees worldwide, 11 of which are known in Israel one of these is reported here for the first time. We found different trends in monotheistic and polytheistic religions concerning their current worship of sacred trees

The supernatural characters and powers of sacred trees in the Holy Land

This article surveys the beliefs concerning the supernatural characteristics and powers of sacred trees in Israel; it is based on a field study as well as a survey of the literature and includes 118 interviews with Muslims and Druze. Both the Muslims and Druze in this study attribute supernatural dimensions to sacred trees which are directly related to ancient, deep-rooted pagan traditions. The Muslims attribute similar divine powers to sacred trees as they do to the graves of their saints; the graves and the trees are both considered to be the abode of the soul of a saint which is the source of their miraculous powers. Any violation of a sacred tree would be strictly punished while leaving the opportunity for atonement and forgiveness. The Druze, who believe in the transmigration of souls, have similar traditions concerning sacred trees but with a different religious background. In polytheistic religions the sacred grove/forest is a centre of the community's official worship; any violation of the trees is regarded as a threat to the well being of the community. Punishments may thus be collective. In the monotheistic world (including Christianity, Islam and Druze) the pagan worship of trees was converted into the worship/adoration of saints/prophets; it is not a part of the official religion but rather a personal act and the punishments are exerted only on the violating individual

Joint modeling of correlated binary outcomes using multivariate logistic regression: contraception and HIV knowledge in Sri Lanka

Reproductive health significantly contributes to the overall well-being and social welfare of women. Within the spectrum of modern and traditional contraceptive methods in use, condoms have been strongly advocated by numerous HIV programs as a primary means of preventing HIV infection in Sri Lanka. Given the intrinsic relationship between contraceptive utilization and HIV awareness, our study aims to concurrently analyze the patterns of contraceptive usage and HIV knowledge, while accounting for their potential correlation. In this study, we introduced the application of the Gumbel type II distribution to effectively capture the interdependence of these joint probabilities, accounting for various covariates. The outcome of simulation studies demonstrated the superior performance of the integrated joint model, in comparison to the separate univariate models. Our findings highlighted several noteworthy risk factors associated with both contraceptive usage and HIV prevention knowledge. These included variables such as residence, level of education, wealth quintile, husband's education level, number of children, engagement with the newspapers, television viewership, and mobile phone usage. The Result indicates a positive association between the adoption of contraception and the awareness of HIV prevention measures, suggesting that individuals who actively embrace contraception are more likely to possess knowledge about preventing HIV transmission

Financial viability of using green roofing in residential buildings

As a result of increased attention towards sustainability worldwide, green concepts have become popular in the construction industry. Green roof is one of the essential elements in a green building that provide many advantages while creating a pleasant appearance for the total building. Green roofs play a major role in energy saving of a building. However, compared to a conventional roof, the initial and maintenance costs of a green roof is quite high due to the additional construction and high maintenance requirements. Thus, this paper compares the Life Cycle Cost (LCC) of a green roof with that of a conventional ceramic tile roof in order to determine the financial viability of green roofing. Findings were gathered from several cases and past researches under initial cost, maintenance cost and energy saving of green roofs and adopted to the selected case for the analysis. Findings of the study indicate that initial cost of the green roof was higher than conventional and represented 8.39:(-6.55) proportion of the total life cycle cost of the building. Similarly, maintenance, operational and replacement costs were also higher than the conventional representing 12.08:(-6.55) proportion of the total life cycle cost of the building. Green roof also had higher energy cost than the conventional, representing (-23.64):(-6.55) proportion of total life cycle cost of the building. As a result, it was found that (-Rs. 11,654.70)/m2 net saving by a green roof is considerable despite of the high initial and the maintenance cost. According to the study, green roofing concept is financially and environmentally beneficial concept even though there are some barriers, like lack of knowledge, lack of techniques, lack of standards in implementing this concept in the Sri Lankan context. Hence, it is recommended to use green roofing in residential buildings

Joint estimation of disease-specific sensitivities and specificities in reader-based multi-disease diagnostic studies of paired organs

<p>Binocular data typically arise in ophthalmology where pairs of eyes are evaluated, through some diagnostic procedure, for the presence of certain diseases or pathologies. Treating eyes as independent and adopting the usual approach in estimating the sensitivity and specificity of a diagnostic test ignores the correlation between fellow eyes. This may consequently yield incorrect estimates, especially of the standard errors. The paper is concerned with diagnostic studies wherein several diagnostic tests, or the same test read by several readers, are administered to identify one or more diseases. A likelihood-based method of estimating disease-specific sensitivities and specificities via hierarchical generalized linear mixed models is proposed to meaningfully delineate the various correlations in the data. The efficiency of the estimates is assessed in a simulation study. Data from a study on diabetic retinopathy are analyzed to illustrate the methodology.</p

Morphometric Analysis of Kala Oya River Basin, Sri Lanka Using Geographical Information Systems

Morphological characteristics of river basins are assessed widely by means of morphometric analysis which is anbsp mathematical quantification of different aspects of river basins. In the present study, Kala Oya River Basin of Sri Lanka was morphometrically analyzed to assess its flood characteristics based on the morphological characteristics with the help of Geographical Information Systems and the methods available in scientific literature. Further, the soil and topographical conditions like land use and terrain characteristics of the basin were also identified. Results of the study revealed that the Kala Oya River Basin has a 6th order river network according to Strahlerrsquos classification with a dendritic drainage pattern and coarse drainage texture. The obtained low values of bifurcation ratio, circularity ratio, elongation ratio and form factor values revealed that a lower and extended peak flow for a longer duration would result from the basin. The drainage density, stream frequency and drainage intensity values indicated that the basin has highly permeable soil, good vegetation cover and lower relief where a low rate of runoff could be resulted reducing the risk for both soil erosion and flooding. The analyzed relief aspects revealed that the basin is less susceptible for severe soil erosion. Confirming the results of the morphometric analysis, the basin area was with 85% of Reddish Brown Earth and Low Humic Gley Soils soil types which are permeable soils and 66% of vegetation cover and a lower relief as more than 95% of the basin was below 200 m and the highest point was also below 1000 m. However, the findings of rainfall analysis for the basin revealed that there were increasing patterns in receiving heavy rainfalls greater than 50 mm/day and maximum rainfall amounts during a day over last 30 years. Similarly, flood occurrences also showed increasing patterns with time. Thus, sustainable management plans should be made in advance to cope with the potential floods that can occur due to high rainfall events and/or due to the degradation of protective vegetation cover, although the studied Kala Oya River Basin is morphometrically capable enough to reduce the flood risk