Enhanced Productivity of a Supported Olefin Trimerization Catalyst

Treatment of dry silica with methylaluminoxane (MAO) followed by (FI)TiCl_3 (FI = (N-(5-methyl-3-(1-adamantyl)salicylidene)-2′-(2″-methoxyphenyl)anilinato) gives a heterogeneous supported ethylene trimerization catalyst, s(FI)Ti, which exhibits productivity more than an order of magnitude higher than its homogeneous analogues. This increase in productivity is attributed to a decreased rate of catalyst decomposition, a process that is proposed to occur via comproportionation to an inactive TiIII species; immobilization retards this process. In addition, s(FI)Ti catalyzes trimerization of α-olefins with high selectivity. Based on regioisomer distributions, catalysis by s(FI)Ti involves the same active species as the previously reported homogeneous systems (FI)TiR_2Me/B(C_6F_5)_3 (R = Me, CH_2SiMe_3, CH_2CMe_3)

Translation controls the expression level of a chimaeric reporter gene

Transcriptional and translational fusions between the reading frame of the β-D-glucuronidase gene (gusA) and the 2′ as well as the 1′ promoter of mannopine synthase (mas), a TR locus of Agrobacterium tumefaciens, were made. The expression of these constructs was studied in the transgenic F1 offspring of independent tobacco transformants at the protein level by assaying for GUS activity and western blot analysis of the GUS protein and at the steady-state mRNA level. In leaves, stems and roots no correlation was found between steady-state levels of GUS mRNA and enzyme activity. In older tissues significantly higher GUS activities were found. This is explained by the stable character of the GUS protein together with an accumulation of protein upon ageing. Three to ten times higher GUS activities were found for in vitro grown plants than for greenhouse-grown plants of the same offspring, despite similar levels of GUS mRNA. Roots from in vitro grown plants display three to ten times higher GUS activities than stems and leaves. In transgenic plants grown in vitro, containing a translational fusion with two AUGs in phase, the initiation of translation in leaf material occurred at both AUGs. Initiation of translation at the first AUG, however, was ten times more frequent. In contrast, initiation in roots from in vitro grown plants occurred exclusively at the second AUG

Continuum reverberation mapping of Mrk 876 over three years with remote robotic observatories

Funding: Research at UC Irvine is supported by NSF grant AST-1907290. HL acknowledges a Daphne Jackson Fellowship sponsored by the Science and Technology Facilities Council (STFC), UK. ERC acknowledges support by the NRF of South Africa. TT acknowledges support from NSF through grant NSF-AST-1907208.Continuum reverberation mapping probes the sizescale of the optical continuum-emitting region in active galactic nuclei (AGN). Through 3 years of multiwavelength photometric monitoring in the optical with robotic observatories, we perform continuum reverberation mapping on Mrk~876. All wavebands show large amplitude variability and are well correlated. Slow variations in the light curves broaden the cross-correlation function (CCF) significantly, requiring detrending in order to robustly recover interband lags. We measure consistent interband lags using three techniques (CCF, JAVELIN, PyROA), with a lag of around 13~days from u to z. These lags are longer than the expected radius of 12~days for the self-gravitating radius of the disk. The lags increase with wavelength roughly following λ4/3, as would be expected from thin disk theory, but the lag normalization is approximately a factor of 3 longer than expected, as has also been observed in other AGN. The lag in the i band shows an excess which we attribute to variable Hα broad-line emission. A flux-flux analysis shows a variable spectrum that follows fν ∝ λ-1/3 as expected for a disk, and an excess in the i band that also points to strong variable Hα emission in that band.Publisher PDFPeer reviewe

New parton distributions in fixed flavour factorization scheme from recent deep-inelastic-scattering data

We present our QCD analysis of the proton structure function $F_2^p(x,Q^2)$ to determine the parton distributions at the next-to-leading order (NLO). The heavy quark contributions to $F_2^i(x,Q^2)$, with $i$ = $c$, $b$ have been included in the framework of the `fixed flavour number scheme' (FFNS). The results obtained in the FFNS are compared with available results such as the general-mass variable-flavour-number scheme (GM-VFNS) and other prescriptions used in global fits of PDFs. In the present QCD analysis, we use a wide range of the inclusive neutral-current deep-inelastic-scattering (NC DIS) data, including the most recent data for charm $F_2^c$, bottom $F_2^b$, longitudinal $F_L$ structure functions and also the reduced DIS cross sections $\sigma_{r,NC}^\pm$ from HERA experiments. The most recent HERMES data for proton and deuteron structure functions are also added. We take into account ZEUS neutral current $e^ \pm p$ DIS inclusive jet cross section data from HERA together with the recent Tevatron Run-II inclusive jet cross section data from CDF and D{\O}. The impact of these recent DIS data on the PDFs extracted from the global fits are studied. We present two families of PDFs, {\tt KKT12} and {\tt KKT12C}, without and with HERA `combined' data sets on $e^{\pm}p$ DIS. We find these are in good agreement with the available theoretical models.Comment: 23 pages, 26 figures and 4 tables. V3: Only few comments and references added in the replaced version, results unchanged. Code can be found at http://particles.ipm.ir/links/QCD.ht

Catching Element Formation In The Act

Gamma-ray astronomy explores the most energetic photons in nature to address some of the most pressing puzzles in contemporary astrophysics. It encompasses a wide range of objects and phenomena: stars, supernovae, novae, neutron stars, stellar-mass black holes, nucleosynthesis, the interstellar medium, cosmic rays and relativistic-particle acceleration, and the evolution of galaxies. MeV gamma-rays provide a unique probe of nuclear processes in astronomy, directly measuring radioactive decay, nuclear de-excitation, and positron annihilation. The substantial information carried by gamma-ray photons allows us to see deeper into these objects, the bulk of the power is often emitted at gamma-ray energies, and radioactivity provides a natural physical clock that adds unique information. New science will be driven by time-domain population studies at gamma-ray energies. This science is enabled by next-generation gamma-ray instruments with one to two orders of magnitude better sensitivity, larger sky coverage, and faster cadence than all previous gamma-ray instruments. This transformative capability permits: (a) the accurate identification of the gamma-ray emitting objects and correlations with observations taken at other wavelengths and with other messengers; (b) construction of new gamma-ray maps of the Milky Way and other nearby galaxies where extended regions are distinguished from point sources; and (c) considerable serendipitous science of scarce events -- nearby neutron star mergers, for example. Advances in technology push the performance of new gamma-ray instruments to address a wide set of astrophysical questions.Comment: 14 pages including 3 figure

Sox17 Promotes Cell Cycle Progression and Inhibits TGF-β/Smad3 Signaling to Initiate Progenitor Cell Behavior in the Respiratory Epithelium

The Sry-related high mobility group box transcription factor Sox17 is required for diverse developmental processes including endoderm formation, vascular development, and fetal hematopoietic stem cell maintenance. Expression of Sox17 in mature respiratory epithelial cells causes proliferation and lineage respecification, suggesting that Sox17 can alter adult lung progenitor cell fate. In this paper, we identify mechanisms by which Sox17 influences lung epithelial progenitor cell behavior and reprograms cell fate in the mature respiratory epithelium. Conditional expression of Sox17 in epithelial cells of the adult mouse lung demonstrated that cell cluster formation and respecification of alveolar progenitor cells toward proximal airway lineages were rapidly reversible processes. Prolonged expression of Sox17 caused the ectopic formation of bronchiolar-like structures with diverse respiratory epithelial cell characteristics in alveolar regions of lung. During initiation of progenitor cell behavior, Sox17 induced proliferation and increased the expression of the progenitor cell marker Sca-1 and genes involved in cell cycle progression. Notably, Sox17 enhanced cyclin D1 expression in vivo and activated cyclin D1 promoter activity in vitro. Sox17 decreased the expression of transforming growth factor-beta (TGF-β)-responsive cell cycle inhibitors in the adult mouse lung, including p15, p21, and p57, and inhibited TGF-β1-mediated transcriptional responses in vitro. Further, Sox17 interacted with Smad3 and blocked Smad3 DNA binding and transcriptional activity. Together, these data show that a subset of mature respiratory epithelial cells retains remarkable phenotypic plasticity and that Sox17, a gene required for early endoderm formation, activates the cell cycle and reinitiates multipotent progenitor cell behavior in mature lung cells

Discovery and Fine-Mapping of Glycaemic and Obesity-Related Trait Loci Using High-Density Imputation

Reference panels from the 1000 Genomes (1000G) Project Consortium provide near complete coverage of common and low-frequency genetic variation with minor allele frequency ≥0.5% across European ancestry populations. Within the European Network for Genetic and Genomic Epidemiology (ENGAGE) Consortium, we have undertaken the fi