The dense gas in the largest molecular complexes of the antennae: HCN and HCO+ observations of NGC 4038/39 using ALMA

We present observations of the dense molecular gas tracers $\mathrm{HCN}$, $\mathrm{HNC}$, and ${\mathrm{HCO}}^{+}$ in the $J=1-0$ transition using the Atacama Large Millimeter/submillimeter Array. We supplement our data sets with previous observations of $\mathrm{CO}$ $J=1-0$, which trace the total molecular gas content. We separate the Antennae into seven bright regions in which we detect emission from all three molecules, including the nuclei of NGC 4038 and NGC 4039, five super giant molecular complexes in the overlap region, and two additional bright clouds. We find that the ratio of ${L}_{\mathrm{HCN}}/{L}_{\mathrm{CO}}$, which traces the dense molecular gas fraction, is greater in the two nuclei (${L}_{\mathrm{HCN}}/{L}_{\mathrm{CO}}$ $\sim \quad 0.07-0.08$) than in the overlap region (${L}_{\mathrm{HCN}}/{L}_{\mathrm{CO}}$ $\lt 0.05$). We attribute this to an increase in pressure due to the stellar potential within the nuclei; a similar effect to what has been seen previously in the Milky Way and nearby spiral galaxies. Furthermore, the ratio of ${L}_{\mathrm{HNC}}/{L}_{\mathrm{HCN}}$ $\sim \quad 0.3-0.4$ does not vary by more than a factor of 1.5 between regions. By comparing our measured ratios to photon dominated region (PDR) models including mechanical heating, we find that the ratio of ${L}_{\mathrm{HNC}}/{L}_{\mathrm{HCN}}$ is consistent with mechanical heating contributing gsim5%–10% of the PDR surface heating to the total heating budget. Finally, the ratio of ${L}_{\mathrm{HCN}}/{L}_{\mathrm{HCO}+}$ varies from ~1 in the nucleus of NGC 4038 down to ~0.5 in the overlap region. The lower ratio in the overlap region may be due to an increase in the cosmic ray rate from the increased supernova rate within this region

Excess baggage for birds: inappropriate placement of tags on gannets changes flight patterns.

Published onlineJournal ArticleResearch Support, Non-U.S. Gov'tThis is the final version of the article. Available from Public Library of Science via the DOI in this record.Devices attached to flying birds can hugely enhance our understanding of their behavioural ecology for periods when they cannot be observed directly. For this, scientists routinely attach units to either birds' backs or their tails. However, inappropriate payload distribution is critical in aircraft and, since birds and planes are subject to the same laws of physics during flight, we considered aircraft aerodynamic constraints to explain flight patterns displayed by northern gannets Sula bassana equipped with (small ca. 14 g) tail- and back-mounted accelerometers and (larger ca. 30 g) tail-mounted GPS units. Tail-mounted GPS-fitted birds showed significantly higher cumulative numbers of flap-glide cycles and a higher pitch angle of the tail than accelerometer-equipped birds, indicating problems with balancing inappropriately placed weights with knock-on consequences relating to energy expenditure. These problems can be addressed by carefully choosing where to place tags on birds according to the mass of the tags and the lifestyle of the subject species.This study would have not been carried out without the financial support from the California Department of Fish and Game's Oil Spill Response Trust Fund (through the Oiled Wildlife Care Network at the Wildlife Health Center, School of Veterinary Medicine, University of California, Davis) and the Royal Society for Prevention of Cruelty to Animals (RSPCA, Wilberforce Way, Southwater, Horsham, West Sussex, RH13 9RS, United Kingdom). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Breeding behavior and pup development of the Caspian seal, Pusa caspica

The Caspian seal, Pusa caspica, is an ice-breeding phocid endemic to the Caspian Sea. The breeding behavior of this species is poorly documented. Here, we report behavioral observations of 518 mother-pup pairs and 210 lone pups, made from the bridge of icebreakers traversing seal breeding grounds while servicing oil installations in the northeastern Caspian Sea, during 34 trips from late January to early March, 2006-2012. The breeding habitat of Caspian seals is land-fast or drift ice, usually at least 20 cm thick, overlying water 3–5 m deep. Pregnant females formed pairs or small groups. They were not observed to use lairs, but preferentially pupped beside ice ridges or ice-slab piles that afforded shelter to pups. In years when there were few natural leads into the ice-field, females often gave birth on the edge of artificial leads formed by shipping channels. Pups were categorized into stages from newborn (1), white-coat (2), molting (3), and molted (4), with Stage 3 and 4 pups appearing from mid to late February. The nursing period lasted at least 3 weeks and neighboring mother-pup pairs appeared to be mutually tolerant. Mothers left their pups alone or at nursery sites, presumably to forage. Most white-coat pups moved over the ice while avoiding water in response to disturbance from vessels. Mother-pup pairs maintained contact while moving across the ice by a combination of the pup’s following response and diligent chaperoning by the mother. During disturbances some lone pups sought refuge in shelters under ice slabs, whereas others followed a neighboring mother-pup pair away from the vessel. Male-female pairing occurred in late season with no male-male competition observed on the ice. While breeding and pup rearing behavior of Caspian seals has some features in common with that of other Holarctic seals, it is largely distinct and adapted to the unique conditions of the Caspian environment, in particular the paucity of snow cover on the ice

Alcohol Production as an Adaptive Livelihood Strategy for Women Farmers in Tanzania and Its Potential for Unintended Consequences on Women's Reproductive Health.

Although women occupy a central position in agriculture in many developing countries, they face numerous constraints to achieving their full potential including unequal access to assets and limited decision-making authority. We explore the intersection of agricultural livelihoods, food and economic security, and women's sexual and reproductive health in Iringa Region, Tanzania. Our goal was to understand whether the benefits of supporting women in the agricultural sector might also extend to more distal outcomes, including sexual and reproductive health. Using the Sustainable Livelihoods Framework to guide data collection, we conducted 13 focus group discussions (FGD) with female (n = 11) and male farmers (n = 2) and 20 in-depth interviews with agricultural extension officers (n = 10) and village agro-dealers (n = 10). Despite providing the majority of agricultural labor, women have limited control over land and earned income and have little bargaining power. In response to these constraints, women adopt adaptive livelihood strategies, such as alcohol production, that allow them to retain control over income and support their households. However, women's central role in alcohol production, in concert with the ubiquitous nature of alcohol consumption, places them at risk by enhancing their vulnerability to unsafe or transactional sex. This represents a dangerous confluence of risk for female farmers, in which alcohol plays an important role in income generation and also facilitates high-risk sexual behavior. Alcohol production and consumption has the potential to both directly and indirectly place women at risk for undesirable sexual and reproductive health outcomes. Group formation, better access to finance, and engaging with agricultural extension officers were identified as potential interventions for supporting women farmers and challenging harmful gender norms. In addition, joint, multi-sectoral approaches from health and agriculture and alternative income-generating strategies for women might better address the complexities of achieving safe and sustainable livelihoods for women in this context

Bilateral Assessment of Functional Tasks for Robot-assisted Therapy Applications

This article presents a novel evaluation system along with methods to evaluate bilateral coordination of arm function on activities of daily living tasks before and after robot-assisted therapy. An affordable bilateral assessment system (BiAS) consisting of two mini-passive measuring units modeled as three degree of freedom robots is described. The process for evaluating functional tasks using the BiAS is presented and we demonstrate its ability to measure wrist kinematic trajectories. Three metrics, phase difference, movement overlap, and task completion time, are used to evaluate the BiAS system on a bilateral symmetric (bi-drink) and a bilateral asymmetric (bi-pour) functional task. Wrist position and velocity trajectories are evaluated using these metrics to provide insight into temporal and spatial bilateral deficits after stroke. The BiAS system quantified movements of the wrists during functional tasks and detected differences in impaired and unimpaired arm movements. Case studies showed that stroke patients compared to healthy subjects move slower and are less likely to use their arm simultaneously even when the functional task requires simultaneous movement. After robot-assisted therapy, interlimb coordination spatial deficits moved toward normal coordination on functional tasks

Assimilation of healthy and indulgent impressions from labelling influences fullness but not intake or sensory experience

Background: Recent evidence suggests that products believed to be healthy may be over-consumed relative to believed indulgent or highly caloric products. The extent to which these effects relate to expectations from labelling, oral experience or assimilation of expectations is unclear. Over two experiments, we tested the hypotheses that healthy and indulgent information could be assimilated by oral experience of beverages and influence sensory evaluation, expected satiety, satiation and subsequent appetite. Additionally, we explored how expectation-experience congruency influenced these factors. Results: Results supported some assimilation of healthiness and indulgent ratings—study 1 showed that indulgent ratings enhanced by the indulgent label persisted post-tasting, and this resulted in increased fullness ratings. In study 2, congruency of healthy labels and oral experience promoted enhanced healthiness ratings. These healthiness and indulgent beliefs did not influence sensory analysis or intake—these were dictated by the products themselves. Healthy labels, but not experience, were associated with decreased expected satiety. Conclusions: Overall labels generated expectations, and some assimilation where there were congruencies between expectation and experience, but oral experience tended to override initial expectations to determine ultimate sensory evaluations and intake. Familiarity with the sensory properties of the test beverages may have resulted in the use of prior knowledge, rather than the label information, to guide evaluations and behaviour

Microbial ligand costimulation drives neutrophilic steroid-refractory asthma

Funding: The authors thank the Wellcome Trust (102705) and the Universities of Aberdeen and Cape Town for funding. This research was also supported, in part, by National Institutes of Health GM53522 and GM083016 to DLW. KF and BNL are funded by the Fonds Wetenschappelijk Onderzoek, BNL is the recipient of an European Research Commission consolidator grant and participates in the European Union FP7 programs EUBIOPRED and MedALL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Cellular responses of Candida albicans to phagocytosis and the extracellular activities of neutrophils are critical to counteract carbohydrate starvation, oxidative and nitrosative stress

Acknowledgments We thank Alexander Johnson (yhb1D/D), Karl Kuchler (sodD/D mutants), Janet Quinn (hog1D/D, hog1/cap1D/D, trx1D/D) and Peter Staib (ssu1D/D) for providing mutant strains. We acknowledge helpful discussions with our colleagues from the Microbial Pathogenicity Mechanisms Department, Fungal Septomics and the Microbial Biochemistry and Physiology Research Group at the Hans Kno¨ll Institute (HKI), specially Ilse D. Jacobsen, Duncan Wilson, Sascha Brunke, Lydia Kasper, Franziska Gerwien, Sea´na Duggan, Katrin Haupt, Kerstin Hu¨nniger, and Matthias Brock, as well as from our partners in the FINSysB Network. Author Contributions Conceived and designed the experiments: PM HW IMB AJPB OK BH. Performed the experiments: PM CD HW. Analyzed the data: PM HW IMB AJPB OK BH. Wrote the paper: PM HW OK AJPB BH.Peer reviewedPublisher PD

Physicochemical analysis of rotavirus segment 11 supports a 'modified panhandle' structure and not the predicted alternative tRNA-like structure (TRLS)

.Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements. Covariation studies and phylogenetic analysis exploring the potential structure of RNA11 of rotaviruses suggested that, besides the previously predicted "modified panhandle" structure, the 5' and 3' termini of one of the isoforms of the bovine rotavirus UKtc strain may interact to form a tRNA-like structure (TRLS). Such TRLSs have been identified in RNAs of plant viruses, where they are important for enhancing replication and packaging. However, using tRNA mimicry assays (in vitro aminoacylation and 3'- adenylation), we found no biochemical evidence for tRNA-like functions of RNA11. Capping, synthetic 3' adenylation and manipulation of divalent cation concentrations did not change this finding. NMR studies on a 5'- and 3'-deletion construct of RNA11 containing the putative intra-strand complementary sequences supported a predominant panhandle structure and did not conform to a cloverleaf fold despite the strong evidence for a predicted structure in this conserved region of the viral RNA. Additional viral or cellular factors may be needed to stabilise it into a form with tRNA-like properties

Migrations and habitat use of the smooth hammerhead shark (Sphyrna zygaena) in the Atlantic Ocean

The smooth hammerhead shark, Sphyrna zygaena, is a cosmopolitan semipelagic shark captured as bycatch in pelagic oceanic fisheries, especially pelagic longlines targeting swordfish and/or tunas. From 2012 to 2016, eight smooth hammerheads were tagged with Pop-up Satellite Archival Tags in the inter-tropical region of the Northeast Atlantic Ocean, with successful transmissions received from seven tags (total of 319 tracking days). Results confirmed the smooth hammerhead is a highly mobile species, as the longest migration ever documented for this species (> 6600 km) was recorded. An absence of a diel vertical movement behavior was noted, with the sharks spending most of their time at surface waters (0-50 m) above 23 degrees C. The operating depth of the pelagic long-line gear was measured with Minilog Temperature and Depth Recorders, and the overlap with the species vertical distribution was calculated. The overlap is taking place mainly during the night and is higher for juveniles (similar to 40% of overlap time). The novel information presented can now be used to contribute to the provision of sustainable management tools and serve as input for Ecological Risk Assessments for smooth hammerheads caught in Atlantic pelagic longline fisheries.Oceanario de Lisboa through Project "SHARK-TAG: Migrations and habitat use of the smooth hammerhead shark in the Atlantic Ocean"; Investigador-FCT from the Portuguese Foundation for Science and Technology (FCT, Fundacao para a Ciencia e Tecnologia) [Ref: IF/00253/2014]; EU European Social Fund; Programa Operacional Potencial Human