First steps in the grape mechanization process in Brazil: quantitative features

Grape harvest is still fully manual in the majority of farms in Brazil (above 99%), yet the structure of the fields and the vine trellis are already prepared for it in a 24% of the cases. Besides, only the large-size farms are prepared for performing a detailed analysis of working capacity, product quality and losses; data of great value when trying to quantitatively address the incorporation of machinery. The fact that grape harvest in South America (and South Africa) be complementary in season compared to Europe, or North America, makes this potential market of Brazil an interesting option for European manufacturers. In this work, we have supervised a whole grape harvest season, in a 552ha farm, where both, mechanical (trailed) and manual harvest, are performed. Harvest performance is assessed by means of digital field notebooks and using georeferenced data (DGPS). A large variety of incidences have been found for the mechanized procedure due mainly to a deficient maintenance of the equipment, yet the increase in work capacity is clear. Also in this study an analysis of juice losses due to mechanized harvest is performed. The quantitative features are defined and have been compared to evaluate the difference among both procedures, together with a technical discussion in the prospective of the grape (harvest) mechanization in the near future in Brazil

Ohmic heating extract of vine pruning residue has anti-colorectal cancer activity and increases sensitivity to the chemotherapeutic drug 5-FU

Vine pruning residues are by-products of the wine industry that have not received much attention in the past, in spite of being rich in bioactive compounds. In this study, we aimed to test whether an ohmic extract of vine pruning residue (VPE) has anti-colorectal cancer (CRC) properties, and whether responses differ according with cell’s mutation profile. VPE decreased human CRC cell proliferation, accompanied by DNA effects and cell cycle modulation. VPE also increased cell sensitivity to the chemotherapeutic drug 5-FU. Our results suggest that tumors harboring BRAF mutations may be more responsive to VPE than KRAS mutated tumors. These effects of the extract were not completely reproduced by the most abundant constituents tested individually at the concentrations present in the effective dose of VPE. Globally, our results indicate that VPE, a polyphenol enriched extract produced by ohmic heating of vine pruning residue, has anti-colorectal cancer potential, including sensitizing to a chemotherapeutical drug, and its use in functional foods or nutraceuticals could be exploited in personalized anti colorectal cancer dietary strategies. Valorization of this lignocellulosic residue should encourage bio-waste recycling, adding value to this agricultural by-product and promoting the sustainable use of natural resources.This research was funded by the Portuguese Foundation for Science and Technology (FCT), under thescope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004)funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte. MSJ was supported by the International Cooperation Program CNPq/CSF at the University of Minho, financed by CNPq-Brazilian Federal Agency and ACC by the INTERACT project–“Integrated ResearchEnvironment, Agro-Chain and Technology”, no. NORTE-01-0145-FEDER-000017, in its line of research entitledISAC, co-financed by the European Regional Development Fund (ERDF), through NORTE 2020 (North RegionalOperational Program 2014/2020).info:eu-repo/semantics/publishedVersio

Evaluation of the radiometric accuracy of images obtained by a Sequoia multispectral camera

Geometric and radiometric corrections of images obtained by remotely piloted aircraft systems (RPAS) are required for the measurement of the spectral reflectance and calculation of vegetation indices. This study aimed to determine which radiometric calibration procedures allow obtaining suborbital images with more accurate reflectance data and spectral indices based on data collected by a Sequoia multispectral camera coupled to a RPAS on black oat cultivations at the flowering stage located in Eldorado do Sul, RS, Brazil. The orthophoto mosaics were processed without and with radiometric corrections by reflectance calibration ground target and/or solar irradiance sensor of the camera, and the radiometric accuracy of these images was evaluated using spectral reflectance ground data collected by the Crop Circle canopy sensor. The results indicated that the use of a reflectance calibration ground target is recommended to increase the radiometric accuracy of photographs even if the Sequoia solar irradiance sensor is employed. The use of the light gray target in the radiometric calibration resulted in orthophotos with the mean square error of reflectance values lower than 0.01 for all the analyzed bands and spectral indices

Investigation of the local environment of SnO2 in an applied magnetic field

This paper presents the results of time-differential perturbed gamma–gamma angular correlation measurements of SnO2 thin films carried out in an applied magnetic field. The measurements were performed upon the implantation of Fe at 80 keV and 111In (111Cd) at 160 keV. The samples were further characterized by energydispersive X-ray spectroscopy. The hyperfine parameters were studied at room temperature with and without an applied magnetic field. The results indicate the presence of two distinct local environments for the probe nuclei. Both occupy a paramagnetic state and correspond to a substitutional Sn site in the rutile phase of SnO2 with different numbers of electrons added to SnO2:Cd0. In addition, the crystal homogeneity of the site 1 increases upon applying the magnetic field

The performance of four molecular methods for the laboratory diagnosis of congenital toxoplasmosis in amniotic fluid samples

Introduction\ud Toxoplasmosis may be life-threatening in fetuses and in immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence of IgM and IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim of this study was to compare the performance of 4 PCR-based methods for the laboratory diagnosis of toxoplasmosis. One hundred pregnant women who seroconverted during pregnancy were included in the study. The definition of cases was based on a 12-month follow-up of the infants.\ud \ud Methods\ud Amniotic fluid samples were submitted to DNA extraction and amplification by the following 4 Toxoplasma techniques performed with parasite B1 gene primers: conventional PCR, nested-PCR, multiplex-nested-PCR, and real-time PCR. Seven parameters were analyzed, sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), negative likelihood ratio (NLR) and efficiency (Ef).\ud \ud Results\ud Fifty-nine of the 100 infants had toxoplasmosis; 42 (71.2%) had IgM antibodies at birth but were asymptomatic, and the remaining 17 cases had non-detectable IgM antibodies but high IgG antibody titers that were associated with retinochoroiditis in 8 (13.5%) cases, abnormal cranial ultrasound in 5 (8.5%) cases, and signs/symptoms suggestive of infection in 4 (6.8%) cases. The conventional PCR assay detected 50 cases (9 false-negatives), nested-PCR detected 58 cases (1 false-negative and 4 false-positives), multiplex-nested-PCR detected 57 cases (2 false-negatives), and real-time-PCR detected 58 cases (1 false-negative).\ud \ud Conclusions\ud The real-time PCR assay was the best-performing technique based on the parameters of Se (98.3%), Sp (100%), PPV (100%), NPV (97.6%), PLR (â^ž), NLR (0.017), and Ef (99%).This work was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo; grant number 2010/15022-1), as well as by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico; grant number 2011-0/471479)

Precision and accuracy of sampling methods in thinned Pinus taeda L. stands

Different sampling methods can be used in forest surveys. It is important to know the precision and accuracy of these sampling methods, and which one is the most appropriate in specific conditions of the forest population. The aim of this study was to compare estimates of a forest inventory performed by different sampling methods with forest census results. The sampling methods evaluated were the fixed-area method and the variable-area methods of Bitterlich, Prodan, and Strand. The data were obtained in a 15-year-old thinned stand of Pinus taeda L., located in the municipality of Teixeira Soares, southern Brazil, with a total area of 12.80 ha. Initially, the forest census was carried out, and subsequently, the sample units for each sampling method were distributed in the stand, with a common starting point. The variables used to compare the sample results with the census means were quadratic diameter, number of trees, basal area, and volume, per hectare. Precision and accuracy were evaluated by sampling error and whether the confidence intervals covered the population means, respectively. The fixed-area and Bitterlich methods stood out in precision for all variables analysed. The fixed-area, Bitterlich and Strand methods with proportion to height provided more accurate estimates. The Prodan method provided inaccurate and imprecise estimates for the variables under analysis, except for the quadratic diameter

Ohmic heating polyphenolic extracts from vine pruning residue with enhanced biological activity

Vine Pruning residue was submitted to conventional heating and ohmic heating (OH) for the extraction of bioactive compounds and analyzed for total phenolic content (TPC), polyphenolic profile, antioxidant activity, antimicrobial activity and anticancer activity. The OH extracts were obtained using Low electric field (496.0 V/cm) or Intermediate electric field - IEF (840.0 V/cm). The tests were performed using 45 % (v/v) ethanol-water extraction solution at 80 °C at different extraction times (20 to 90 min). The extract that stood out among the others concerning anticancer potential was the one obtained by OH when used, IEF, where the TPC was significantly higher than in the other extracts which correlated with higher antioxidant, antimicrobial and anti-proliferative activity on different tumor cell lines (HepG2, MDA-MB-231, MCF-7 and Caco2). Vine pruning OH extracts obtained using green solvents by an eco-friendly procedure were revealed as a source of compounds with relevant antioxidant and anticancer activity.This work has been carried out at the Biomass and Bioenergy Research Infrastructure (BBRI)- LISBOA-01-0145-FEDER-022059, supported by Operational Programme for Competitiveness and Internationalization (PORTUGAL2020), by Lisbon Portugal Regional Operational Programme (Lisboa 2020) and by North Portugal Regional Operational Programme (Norte 2020) under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and has been supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Meirielly S. Jesus thanks her fellowship supported by the International Cooperation Program CNPq/CSFat the University of Minho financed by CNPq-Brazilian Federal Agency. Zlatina Genisheva thanks to FCT for the financial support (ref. SFRH/BPD/108868/2015). Ana Cristina Carvalho was supported by the INTERACT project – “Integrated Research Environment, Agro-Chain and Technology”, no. NORTE-01-0145-FEDER-000017, in its line of research entitled ISAC, co-financed by the European Regional Development Fund (ERDF) through NORTE 2020 (North Regional Operational Program 2014/2020).info:eu-repo/semantics/publishedVersio

Tea: aspects related to the quality and prospects

Black tea is one of the most widely consumed beverages in the world and it is prepared by the infusion of water with processed leaves of Camellia sinensis, which is cultivated in more than 30 countries, assuming a great socioeconomic importance. Several studies suggest that tea has protective effects against cancer and cardiovascular disease, due to the presence of polyphenols called catechins, which are oxidized enzymatically during the processing of the black tea, generating a mixture consisting mainly of theaflavins, theasinensins and thearubigins. The world production of tea has increased more than the demand, causing a drop of the price which, together with the increase in cultivation costs has shown a need for high productivity and quality. Nevertheless, there is not yet an efficient and accurate criteria to predict the quality of tea and agronomic practices which contribute to the increase in quality. In Brazil, the culture of tea is concentrated in the Ribeira Valley - SP, and almost all the production is exported. Despite the Brazilian product is not of high quality, it has achieved good prices in the international market. The Brazilian production, the production area and the number of tea industries are decreasing in recent years, clearly indicating the need for investments.O chá preto é uma das bebidas mais consumidas no mundo. Essa bebida é feita a partir da infusão de folhas processadas de Camellia sinensis, que é cultivada em mais de 30 países, tendo grande importância socioeconômica. Estudos sugerem que o chá tem efeito protetor contra diversos tipos de câncer e doenças cardiovasculares devido à presença de polifenóis denominados catequinas, que são oxidadas enzimaticamente durante o processamento das folhas, gerando uma mistura constituída principalmente de teaflavinas, teasinensinas e tearubiginas. A produção mundial de chá tem aumentado mais do que o consumo, provocando redução do preço que, juntamente com o aumento no custo de produção, implica a necessidade de alta produtividade e qualidade. Apesar disso, ainda não foram estabelecidos critérios precisos e eficientes para predizer a qualidade do chá a partir das folhas, bem como quais práticas agronômicas contribuem para o aumento da qualidade. No Brasil, a cultura do chá se concentra no Vale do Ribeira, em São Paulo (SP), sendo quase toda produção exportada. Apesar de o produto brasileiro não ser de alta qualidade, tem conseguido bons preços no mercado internacional. A produção brasileira, a área de produção e o número de indústrias de chá vêm diminuindo ao longo dos últimos anos, o que demonstra a necessidade de investimentos.12581266Fundação para o Desenvolvimento da UNESP (FUNDUNESP

RESPOSTA À CALAGEM DA Brachiaria brizantha SUBMETIDA A DIFERENTES DOSES DE ADUBAÇÃO NITROGENADA

A produção de bovinos em pastagens é caracterizada no Brasil como uma condição de exploração extrativista, em solos exauridos por outras culturas, pela erosão ou de baixa fertilidade natural, ocasionando baixas produtividades. O trabalho teve objetivo principal avaliar os efeitos da calagem e da adubação nitrogenada em Brachiaria brizantha, cultivar Xaraés, avaliando a produção e a elevação dos teores de Nitrogênio e Cálcio na planta. O experimento foi conduzido em casa de vegetação na UNOESTE, no período de agosto a novembro de 2003. Foram distribuídas 20 sementes por vaso e aos 44 dias após a semeadura aplicaram-se as doses de nitrogênio e 78 dias após o plantio realizou-se o corte da parte aérea para determinação da matéria seca. A aplicação de calcário e nitrogênio proporcionou um aumento significativo na produção da forrageira, além de elevar significativamente o teor de Nitrogênio e Cálcio na planta. Assim fica evidente a importância da adubação em culturas forrageiras para a obtenção de altas produtividades e maior lotação por hectare