Moving Ground, Breaking Traditions: Tasha\u27s Chronicle

This Note uses a fictional dialogue to analyze and engage issues concerning stereotypes, stigmas, and affirmative action. It also highlights the importance of role models for students of color and the disparate hiring practices of law firms and legal employers through the conversations and thoughts of its main character, Tasha Crenshaw

STED microscopy in the visible range

Light microscopy is a key scientific instrument in the life sciences. However, the resolution of far-field light microscopy is limited by diffraction. Exploiting a saturated depletion of the molecular excited state by stimulated emission, stimulated emission depletion (STED) breaks the resolution barrier in the important subfield of fluorescence microscopy. To this end, STED microscopy utilizes a doughnut-shaped beam featuring a central zero which is capable of quenching the fluorescence solely in the focal periphery. While STED microscopy was initially restricted to near infrared-emitting fluorophores, in this thesis STED microscopy is shown to be viable with visible (green-yellow-red) fluorophores. In particular, STED is established with the green and yellow fluorescent proteins (GFP, YFP) which are endogenous cellular markers of outstanding biological importance. The spectral conditions for STED with these fluorophores are given. The expansion of STED to the visible range has enabled the first application to biophysics and to addressing unsolved problems of cell biology. For example, STED microscopy revealed that the synaptic vesicle protein synaptotagmin remains an integral patch following fusion with the plasma membrane. Moreover, membrane microdomains were resolved with unprecedented (65 nm) spatial resolution. Furthermore, a novel doughnut-shape of the STED beam utilizing a helical phase ramp and a central singularity was established for STED. Finally, due to the smaller wavelength for stimulated emission, the viability of STED with visible dyes pushes the resolution down to smaller attainable values

Cabinet Gorge Dam Spillway Modifications for TDG Abatement - Design Evolution and Field Performance

Avista is implementing spillway modifications to reduce TDG supersaturation downstream of Cabinet Gorge Dam.The key feature of the modifications is the addition of roughness elements, similar to supercavitating baffle blocks,to break up the spillway jet thereby reducing the depth of plunge and TDG supersaturation. The work is progressing in a step-wise manner. A single bay was modified in 2012 and field tested in 2013. Following the initial field tests, aCFD model was developed to aid in design refinements for the prototype and to improve the design prior to modification of subsequent spillway bays. The prototype demonstrated that spillway modifications are an effective method to reduce TDG downstream of a spillway discharging freely into a deep plunge pool. The CFD model has allowed the design to be simplified while maintaining the plunge depth improvements of the initial prototype and reducing the effect of the modifications on spillway capacity. This paper presents the prototype design and the design that will be implemented for the next two bays, summarizes the results from the prototype field tests, and describes the CFD model and results

Reflections on Presumed Incompetent: The Intersections of Race and Class for Women in Academia Symposium--The Plenary Panel

Presumed Incompetent was produced thanks to the vision and commitment of its editors: Dr. Gabriella Gutiérrez y Muhs, Dr. Yolanda Flores Niemann, Carmen G. González, and Angela P. Harris. This symposium came to fruition because the Berkeley Journal of Gender, Law & Justice invited the two law professor editors, Professor Harris and Professor González, to convene a distinguished group of scholars from Canada and the United States to expand and deepen the conversation initiated by the book. The very successful day-long symposium and the publication of the resulting articles were made possible by the resources, time, and dedication provided by the University of California Berkeley School of Law, the Berkeley Journal of Gender, Law & Justice, the Seattle Journal for Social Justice, the Thelton E. Henderson Center for Social Justice, and the generous support of the law firm of Munger, Tolles & Olson. Finally, the audience, a mix of academics and students, supported the symposium and traveled from all over the United States to attend and be a part of a historical event where we acknowledged the pain and victories of colleagues, and recognized that there is still much work to be done. The plenary panel proceeded as follows. First, the panelists gave brief opening remarks about their chapters, followed by a question and answer portion, and ended with my closing remarks. Members of the audience submitted questions once the plenary panel discussion began. While this Article is not a verbatim transcript of the plenary panel, all the questions are the same ones posed during the panel. The remarks and answers included here follow a semi-transcript format that allowed the moderator and panelists an opportunity to elaborate further on some of the comments and responses

Sporting embodiment: sports studies and the (continuing) promise of phenomenology

Whilst in recent years sports studies have addressed the calls ‘to bring the body back in’ to theorisations of sport and physical activity, the ‘promise of phenomenology’ remains largely under-realised with regard to sporting embodiment. Relatively few accounts are grounded in the ‘flesh’ of the lived sporting body, and phenomenology offers a powerful framework for such analysis. A wide-ranging, multi-stranded, and interpretatively contested perspective, phenomenology in general has been taken up and utilised in very different ways within different disciplinary fields. The purpose of this article is to consider some selected phenomenological threads, key qualities of the phenomenological method, and the potential for existentialist phenomenology in particular to contribute fresh perspectives to the sociological study of embodiment in sport and exercise. It offers one way to convey the ‘essences’, corporeal immediacy and textured sensuosity of the lived sporting body. The use of Interpretative Phenomenological Analysis (IPA) is also critically addressed. Key words: phenomenology; existentialist phenomenology; interpretative phenomenological analysis (IPA); sporting embodiment; the lived-body; Merleau-Pont

Balancing professional tension and deciding upon the status of death : making end-of-life decisions in intensive care units

This study investigated how intensivists make decisions regarding withholding and withdrawing treatment for patients at the end of their lives. This involved completing in-depth interviews from two sites of the South of England, United Kingdom by twelve intensivists. The data collected by these intensivists were analysed using thematic analysis. This resulted in the identification of three themes: intensivists’ role, treatment effectiveness, and patients’ best interest. Transcending these were two overarching themes relating to the balance between quantity and quality of life, and the intensivists’ sense of responsibility versus burden. The results are considered in terms of making sense of death and the role of beliefs in the decision-making process

'It seemed churlish not to': How living non-directed kidney donors construct their altruism

Our objective was to explore how prospective altruistic kidney donors construct their decision to donate. Using a qualitative design and biographical-narrative semistructured interviews, we aimed to produce text for analysis on two levels: the social implications for subjectivity and practice and a tentative psychodynamic explanation of the participants’ psychological investment in the discourses they used. A total of six prospective altruistic kidney donors were interviewed. A psychosocial approach to the analysis was taken. In-depth discourse analysis integrated Foucauldian with psychodiscursive approaches and psychodynamic theory was applied to sections of text in which participants seemed to have particular emotional investment. Analysis generated three major discursive themes: other-oriented, rational and self-oriented discourses. The desire to donate was experienced as compelling by participants. Participants used discourses to position themselves as concerned with the needs of the recipient, to resist questioning and criticism, and to manage difficult feelings around mortality. Participants tended to reject personal motivations for altruistic donation, positioning relatives’ disapproval as selfish and illogical. These results suggest that the term ‘altruistic’ for living non-directed organ donation constrains available discourses, severely limiting what can be said, felt, thought and done by donors, clinicians and the public. A more useful approach would acknowledge potential psychological motives and gains for the donor