A Luminous Red Nova in M31 and its Progenitor System

We present observations of M31LRN 2015 (MASTER OT J004207.99+405501.1), discovered in M31 in January 2015, and identified as a rare and enigmatic luminous red nova (LRN). Spectroscopic and photometric observations obtained by the Liverpool Telescope showed the LRN becoming extremely red as it faded from its M(V) = -9.4 +/- 0.2 peak. Early spectra showed strong Halpha emission that weakened over time as a number of absorption features appeared, including Na I D and Ba II. At later times strong TiO absorption bands were also seen. A search of archival Hubble Space Telescope data revealed a luminous red source to be the likely progenitor system, with pre-outburst Halpha emission also detected in ground-based data. The outburst of M31LRN 2015 shows many similarities, both spectroscopically and photometrically, with that of V838 Mon, the best studied LRN. We finally discuss the possible progenitor scenarios

On the Progenitors of Local Group Novae. II. The Red Giant Nova Rate of M31

In our preceding paper, Liverpool Telescope data of M31 novae in eruption were used to facilitate a search for their progenitor systems within archival Hubble Space Telescope (HST) data, with the aim of detecting systems with red giant secondaries (RG-novae) or luminous accretion disks. From an input catalog of 38 spectroscopically confirmed novae with archival quiescent observations, likely progenitors were recovered for eleven systems. Here we present the results of the subsequent statistical analysis of the original survey, including possible biases associated with the survey and the M31 nova population in general. As part of this analysis we examine the distribution of optical decline times (t(2)) of M31 novae, how the likely bulge and disk nova distributions compare, and how the M31 t(2) distribution compares to that of the Milky Way. Using a detailed Monte Carlo simulation, we determine that 30 (+13/-10) percent of all M31 nova eruptions can be attributed to RG-nova systems, and at the 99 percent confidence level, >10 percent of all M31 novae are RG-novae. This is the first estimate of a RG-nova rate of an entire galaxy. Our results also imply that RG-novae in M31 are more likely to be associated with the M31 disk population than the bulge, indeed the results are consistent with all RG-novae residing in the disk. If this result is confirmed in other galaxies, it suggests any Type Ia supernovae that originate from RG-nova systems are more likely to be associated with younger populations, and may be rare in old stellar populations, such as early-type galaxies

Improved mixing height monitoring through a combination of lidar and radon measurements

Surface-based radon (222Rn) measurements can be combined with lidar backscatter to obtain a higher quality time series of mixing height within the planetary boundary layer (PBL) than is possible from lidar alone, and a more quantitative measure of mixing height than is possible from only radon. The reason why lidar measurements are improved is that there are times when lidar signals are ambiguous, and reliably attributing the mixing height to the correct aerosol layer presents a challenge. By combining lidar with a mixing length scale derived from a time series of radon concentration, automated and robust attribution is possible during the morning transition. Radon measurements provide mixing information during the night, but concentrations also depend on the strength of surface emissions. After processing radon in combination with lidar, we obtain nightly measurements of radon emissions and are able to normalise the mixing length scale for changing emissions. After calibration with lidar, the radonderived equivalent mixing height agrees with other measures of mixing on daily and hourly timescales and is a potential method for studying intermittent mixing in nocturnal boundary layers.© 2013, Copernicus Publications

Evidence of Interaction between Polychlorinated Biphenyls and Phthalates in Relation to Human Sperm Motility

Previously, we reported evidence of inverse associations between exposure to some polychlorinated biphenyls (PCBs) and some phthalate monoesters in relation to semen parameters, specifically sperm motility. Because humans are exposed to both phthalates and PCBs and because experimental studies suggest that PCBs may interact with glucuronidative enzymes that are responsible for phthalate metabolism, we explored the potential interaction between phthalates and PCBs in relation to human semen quality. We studied 303 men who were partners in subfertile couples seeking infertility diagnosis from the andrology laboratory at Massachusetts General Hospital. Semen parameters were dichotomized based on World Health Organization reference values, and phthalate and PCB levels were dichotomized at their respective medians. After adjusting for age and abstinence time, for below reference sperm motility there was a greater than additive interaction between monobenzyl phthalate and PCB-153 [relative excess risk due to interaction (RERI) = 1.40; 95% confidence interval (CI), 0.41–3.22], sum of PCBs (RERI = 1.24; 95% CI, 0.15–2.94), and cytochrome P450 (CYP450)-inducing PCBs (RERI = 1.30; 95% CI, 0.21–3.06). For below-reference sperm motility, there was also a greater than additive interaction between monobutyl phthalate (MBP) and PCB-153 (RERI = 1.42; 95% CI, 0.09–3.76) and CYP450-inducing PCBs (RERI = 1.87; 95% CI, 0.56–4.52) and a suggestive interaction between MBP and sum of PCBs (RERI = 1.35; 95% CI, −0.11 to 3.48). In conclusion, because there are important risk assessment and public health implications of interactions between these two ubiquitous classes of compounds, further studies need to be conducted to confirm these results and identify potential mechanisms of interactions

An International Working Definition for Quality of Oral Healthcare.

KNOWLEDGE TRANSFER STATEMENT: This special communication describes the development of a working definition for quality of oral healthcare. The findings of this study are intended to raise awareness of the relevance of quality improvement initiatives in oral healthcare. The working definition described here has the potential to facilitate further conversations and activities aiming at quality improvement in oral healthcare

Consumer perceptions and purchasing of packaged water products in Sierra Leone

Introduction: access to improved sources of drinking water remains a complex challenge in Sierra Leone and other low and middle income countries. We aimed to qualitatively examine consumer perceptions and purchasing behaviors of packaged water products in Sierra Leone. Methods: we conducted 25 focus groups with 178 consumers and petty traders of packaged water across the four geographic regions of Sierra Leone. Discussions were recorded, transcribed, and coded into themes. The Health Belief Model guided the thematic data analysis. Results: packaged water was broadly perceived as safe, accessible, and convenient. Participants who lived outside of the capital city, Freetown, were more likely to report cost as a barrier. Personal experiences with a brand moderated trust levels. Self-reported handling behaviors of PW products were generally unhygienic. There was widespread belief that packaged water keeps newborn babies healthy. Consumers desired a simple mechanism to better identify government approved PW products. Conclusion: perceived risks, benefits, barriers, self-efficacy, and reinforcing cues to action qualitatively influenced consumers’ purchasing behavior of packaged water. Government regulators should provide consumers with reliable means to identify approved packaged water products. Consumer education efforts should include hygienic handling of packaged water products in order to minimize post-production contamination

Tectono‐Stratigraphic Evolution of the Kerguelen Large Igneous Province: The Conjugate William’s Ridge‐Broken Ridge Rifted Margins

AbstractExtensive investigation of continental rift systems has been fundamental for advancing the understanding of extensional tectonics and modes of formation of new ocean basins. However, current rift classification schemes do not account for conjugate end members formed by Large Igneous Province crust, referring to thick mafic crust, sometimes including continental fragments. Here, we investigate the rifting of William's Ridge (Kerguelen Plateau) and Broken Ridge, components of the Kerguelen Large Igneous Province now situated in the Southeast Indian Ocean, and incorporate these end members into the deformation migration concept for rifted margins. We use multichannel seismic reflection profiles and data from scientific drill cores acquired on both conjugate margins to propose, for the first time, a combined tectono‐stratigraphic framework. We interpret seismic patterns, tectonic features, and magnetic anomaly picks to determine an across‐strike structural domain classification. This interpretation considers the rift system overall to be “magma‐poor” despite being located proximal to the Kerguelen plume but suggests that syn‐rift interaction between the Kerguelen mantle plume and the lithospheric structure of William's Ridge and Broken Ridge has controlled the along‐strike segmentation of both conjugates. We integrate seismic reflection and bathymetric data to test the hypothesis of predominantly transform motion, between the Australian and Antarctic plates, in Late Cretaceous and Paleogene time.</jats:p

Effectiveness of moving on: an Australian designed generic self-management program for people with a chronic illness

Background: This paper presents the evaluation of “Moving On”, a generic self-management program for people with a chronic illness developed by Arthritis NSW. The program aims to help participants identify their need for behavior change and acquire the knowledge and skills to implement changes that promote their health and quality of life. Method: A prospective pragmatic randomised controlled trial involving two group programs in community settings: the intervention program (Moving On) and a control program (light physical activity). Participants were recruited by primary health care providers across the north-west region of metropolitan Sydney, Australia between June 2009 and October 2010. Patient outcomes were self-reported via pre- and post-program surveys completed at the time of enrolment and sixteen weeks after program commencement. Primary outcomes were change in self-efficacy (Self-efficacy for Managing Chronic Disease 6-Item Scale), self-management knowledge and behaviour and perceived health status (Self-Rated Health Scale and the Health Distress Scale). Results: A total of 388 patient referrals were received, of whom 250 (64.4%) enrolled in the study. Three patients withdrew prior to allocation. 25 block randomisations were performed by a statistician external to the research team: 123 patients were allocated to the intervention program and 124 were allocated to the control program. 97 (78.9%) of the intervention participants commenced their program. The overall attrition rate of 40.5% included withdrawals from the study and both programs. 24.4% of participants withdrew from the intervention program but not the study and 22.6% withdrew from the control program but not the study. A total of 62 patients completed the intervention program and follow-up evaluation survey and 77 patients completed the control program and follow- up evaluation survey. At 16 weeks follow-up there was no significant difference between intervention and control groups in self-efficacy; however, there was an increase in self-efficacy from baseline to follow-up for the intervention participants (t=−1.948, p=0.028). There were no significant differences in self-rated health or health distress scores between groups at follow-up, with both groups reporting a significant decrease in health distress scores. There was no significant difference between or within groups in self-management knowledge and stage of change of behaviours at follow-up. Intervention group attenders had significantly higher physical activity (t=−4.053, p=0.000) and nutrition scores (t=2.315, p= 0.01) at follow-up; however, these did not remain significant after adjustment for covariates. At follow-up, significantly more participants in the control group (20.8%) indicated that they did not have a self-management plan compared to those in the intervention group (8.8%) (X2=4.671, p=0.031). There were no significant changes in other self-management knowledge areas and behaviours after adjusting for covariates at follow-up. Conclusions: The study produced mixed findings. Differences between groups as allocated were diluted by the high proportion of patients not completing the program. Further monitoring and evaluation are needed of the impact and cost effectiveness of the program. Trial registration: Australian New Zealand Clinical Trials Registry: ACTRN1260900029821

In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR

BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage that blocks polymerase elongation, but an insidious possibility is error prone translesion synthesis across sites of damage, producing in vitro artifactual mutations during PCR. METHODS: To better understand the consequences of fixation, DNA specimens extracted from fresh or fixed tissues were amplified with Taq DNA polymerase, and their PCR products were cloned and sequenced. RESULTS: Significantly more (3- to 4-fold) mutations were observed with fixed DNA specimens. The majority of mutations were transitions, predominantly at A:T base pairs, randomly distributed along the template. CONCLUSIONS: Formalin fixation appears to cause random base damage, which can be bridged during PCR by Taq DNA polymerase through error prone translesion synthesis. Fixed DNA is a damaged but "readable" template

Effective bioreduction of hexavalent chromium–contaminated water in fixed-film bioreactors

Hexavalent chromium (Cr6+) contamination from a dolomite stone mine in Limpopo Province, South Africa, has resulted in extensive groundwater contamination. In order to circumvent any further negative environmental impact at this site, an effective and sustainable treatment strategy for the removal of up to 6.49 mg/. Cr6+ from the groundwater was developed. Laboratory-scale, continuous up-flow bioreactors were constructed to  evaluate reduction of Cr6+, with a residence time of 24 h, an efficiency  porosity of 44% and a flow rate of 1.5 m./min. Stoichiometrically balancing terminal electron acceptors in the feed water with a selected electron donor, directed reactor balance for complete Cr6+ reduction. The microbial  community shifted in relative dominance during operation to establish an optimal metal-reducing community, including Enterobacter cloacae,  Flavobacterium sp. and Ralstonia sp., which achieved 100% reduction. Evaluation after reactor termination with SEM-EDX and XRD confirmed the establishment of biofilm on the reactor matrix, as well as trivalent  chromium (Cr3+) precipitation within the reactor. Due to gravitational force, high concentrations of Cr3+ were found in the bottom third of the reactor. Based on the results from the laboratory investigation, a 24 000 .  fixed-film pilot bioreactor was designed and constructed at this site. Influent flow rates, electron donor injection and automated sampling were remotely controlled by a programmable logic controller (PLC). Similar to the laboratory column study, steady state conditions could be achieved and successful Cr6+ reduction was evident. This is the first up-scaled, effective demonstration of a biological chromium(VI) bioremediation system in South Africa.Keywords: Bioreduction, fixed-film reactor, hexavalent chromium, microbial diversit