342 research outputs found
Identification of Highly Expressed, Soluble Proteins Using an Improved, High-Throughput Pooled ORF Expression Technology
This article describes an improved pooled open reading frame (ORF) expression technology (POET) that uses recombinational cloning and solution-based tandem mass spectrometry (MS/MS) to identify ORFs that yield high levels of soluble, purified protein when expressed in Escherichia coli. Using this method, three identical pools of 512 human ORFs were subcloned, purified, and transfected into three separate E. coli cultures. After bulk expression and purification, the proteins from the three separate pools were digested into tryptic peptides. Each of these samples was subsequently analyzed in triplicate using reversed-phase high-performance liquid chromatography (LC) coupled directly online with MS/MS. The abundance of each protein was determined by calculating the average exponentially modified protein abundance index (emPAI) of each protein across the three protein pools. Human proteins that consistently gave high emPAI values were subjected to small-scale expression and purification. These clones showed high levels of expression of soluble protein. Conversely, proteins that were not observed by LC-MS/MS did not show any detectable soluble expression in small-scale validation studies. Using this improved POET method allows the expression characteristics of hundreds of proteins to be quickly determined in a single experiment
Evidence for a correlation between the sizes of quiescent galaxies and local environment to z ~ 2
We present evidence for a strong relationship between galaxy size and
environment for the quiescent population in the redshift range 1 < z < 2.
Environments were measured using projected galaxy overdensities on a scale of
400 kpc, as determined from ~ 96,000 K-band selected galaxies from the UKIDSS
Ultra Deep Survey (UDS). Sizes were determined from ground-based K-band
imaging, calibrated using space-based CANDELS HST observations in the centre of
the UDS field, with photometric redshifts and stellar masses derived from
11-band photometric fitting. From the resulting size-mass relation, we confirm
that quiescent galaxies at a given stellar mass were typically ~ 50 % smaller
at z ~ 1.4 compared to the present day. At a given epoch, however, we find that
passive galaxies in denser environments are on average significantly larger at
a given stellar mass. The most massive quiescent galaxies (M_stellar > 2 x
10^11 M_sun) at z > 1 are typically 50 % larger in the highest density
environments compared to those in the lowest density environments. Using Monte
Carlo simulations, we reject the null hypothesis that the size-mass relation is
independent of environment at a significance > 4.8 sigma for the redshift range
1 < z < 2. In contrast, the evidence for a relationship between size and
environment is much weaker for star-forming galaxies.Comment: Accepted for publication in MNRAS. 16 pages, 11 figures, 6 table
Structure and Colors of Diffuse Emission in the Spitzer Galactic First Look Survey
We investigate the density structure of the interstellar medium using new
high-resolution maps of the 8 micron, 24 micron, and 70 micron surface
brightness towards a molecular cloud in the Gum Nebula, made as part of the
Spitzer Space Telescope Galactic First Look Survey. The maps are correlated
with 100 micron images measured with IRAS. At 24 and 70 micron, the spatial
power spectrum of surface brightness follows a power law with spectral index
-3.5. At 24 micron, the power law behavior is remarkably consistent from the
0.2 degree size of our maps down to the 5 arcsecond spatial resolution. Thus,
the structure of the 24 micron emission is self-similar even at milliparsec
scales. The combined power spectrum produced from Spitzer 24 micron and IRAS 25
micron images is consistent with a change in the power law exponent from -2.6
to -3.5. The decrease may be due to the transition from a two-dimensional to
three-dimensional structure. Under this hypothesis, we estimate the thickness
of the emitting medium to be 0.3 pc.Comment: 13 Pages, 3 Figures, to be published in Astrophysical Journal
Supplement Series (Spitzer Special Issue), volume 154. Uses aastex v5.
Discovery and Characterization of Bacteriophage LuckyBarnes
Here, we report the genome sequence of LuckyBarnes, a newly isolated singleton siphovirus that infects Brevibacterium iodinum ATCC 15728 and has a 50,774-bp genome with 67 predicted genes
The USNO-B Catalog
USNO-B is an all-sky catalog that presents positions, proper motions,
magnitudes in various optical passbands, and star/galaxy estimators for
1,042,618,261 objects derived from 3,643,201,733 separate observations. The
data were obtained from scans of 7,435 Schmidt plates taken for the various sky
surveys during the last 50 years. USNO-B1.0 is believed to provide all-sky
coverage, completeness down to V = 21, 0.2 arcsecond astrometric accuracy at
J2000, 0.3 magnitude photometric accuracy in up to five colors, and 85%
accuracy for distinguishing stars from non-stellar objects. A brief discussion
of various issues is given here, but the actual data are available from
http://www.nofs.navy.mil and other sites.Comment: Accepted by Astronomical Journa
Design of 280 GHz feedhorn-coupled TES arrays for the balloon-borne polarimeter SPIDER
We describe 280 GHz bolometric detector arrays that instrument the
balloon-borne polarimeter SPIDER. A primary science goal of SPIDER is to
measure the large-scale B-mode polarization of the cosmic microwave background
in search of the cosmic-inflation, gravitational-wave signature. 280 GHz
channels aid this science goal by constraining the level of B-mode
contamination from galactic dust emission. We present the focal plane unit
design, which consists of a 1616 array of conical, corrugated feedhorns
coupled to a monolithic detector array fabricated on a 150 mm diameter silicon
wafer. Detector arrays are capable of polarimetric sensing via waveguide
probe-coupling to a multiplexed array of transition-edge-sensor (TES)
bolometers. The SPIDER receiver has three focal plane units at 280 GHz, which
in total contains 765 spatial pixels and 1,530 polarization sensitive
bolometers. By fabrication and measurement of single feedhorns, we demonstrate
14.7 FHWM Gaussian-shaped beams with 1% ellipticity in a 30%
fractional bandwidth centered at 280 GHz. We present electromagnetic
simulations of the detection circuit, which show 94% band-averaged,
single-polarization coupling efficiency, 3% reflection and 3% radiative loss.
Lastly, we demonstrate a low thermal conductance bolometer, which is
well-described by a simple TES model and exhibits an electrical noise
equivalent power (NEP) = 2.6 10 W/,
consistent with the phonon noise prediction.Comment: Proceedings of SPIE Astronomical Telescopes + Instrumentation 201
Infrared Excess and Molecular Clouds: A comparison of new suerveys of far-infrared and H I 21-cm emission at high galactic latitudes
We have created a map of the large-scale infrared surface brightness in
excess of that associated with the atomic interstellar medium, using
region-by-region correlations between the far-infrared and 21-cm line surface
brightness. Our study updates and extends a previous attempt with IRAS and
Berkeley/Parkes H I surveys. The far-infrared observations used here are from
DIRBE, which extends far-infrared wavelength coverage to 240 um, so that we are
reliably sampling the emission of large, thermal-equilibrium grains that
dominate the dust mass. The H I data are from the combined Leiden-Dwingeloo and
Parkes 21-cm line surveys. Using the maps of excess infrared emission at 100,
140, and 240 um, we created an atlas and identified the coherent structures.
These infrared excess clouds can be caused both by dust that is warmer than
average, or by dust associated with gas other than the atomic interstellar
medium. We find very few warm clouds, such as the H II region around Spica. The
majority of the infrared excess clouds are colder than the average atomic
interstellar medium. These clouds are peaks of column density, and their excess
infrared emission is due to dust associated with molecular gas. We identify
essentially all known high-latitude molecular clouds in the infrared excess
maps, and further identify a sample of new clouds with similar infrared
properties. The infrared excess was correlated with CO line brightness,
allowing us to measure the ratio of N(H2)/W(CO) for high-latitude clouds. The
atlas of infrared excess may be a useful guide to regions of relatively high
column density, which might cause high extinction toward extragalactic objects
at optical and ultraviolet wavelengths and confusion for cosmic infrared and
microwave backgrounds.Comment: * manuscript in 3 TeX files, 12 figures in 15 gif files submitted to
the Astrophysical Journal on Feb. 12, 1998 * this version contains a new
version of Figs. 7-8, with the correct cloud name
Broad clinical phenotypes associated with TAR-DNA binding protein (TARDBP) mutations in amyotrophic lateral sclerosis
The finding of TDP-43 as a major component of ubiquitinated protein inclusions in amyotrophic lateral sclerosis (ALS) has led to the identification of 30 mutations in the transactive response-DNA binding protein (TARDBP) gene, encoding TDP-43. All but one are in exon 6, which encodes the glycine-rich domain. The aim of this study was to determine the frequency of TARDBP mutations in a large cohort of motor neurone disease patients from Northern England (42 non-superoxide dismutase 1 (SOD1) familial ALS (FALS), nine ALS-frontotemporal dementia, 474 sporadic ALS (SALS), 45 progressive muscular atrophy cases). We identified four mutations, two of which were novel, in two familial (FALS) and two sporadic (SALS) cases, giving a frequency of TARDBP mutations in non-SOD1 FALS of 5% and SALS of 0.4%. Analysis of clinical data identified that patients had typical ALS, with limb or bulbar onset, and showed considerable variation in age of onset and rapidity of disease course. However, all cases had an absence of clinically overt cognitive dysfunction
Galaxy Zoo: CANDELS barred discs and bar fractions
The formation of bars in disc galaxies is a tracer of the dynamical maturity of the population. Previous studies have found that the incidence of bars in discs decreases from the local Universe to z ~ 1, and by z > 1 simulations predict that bar features in dynamically mature discs should be extremely rare. Here, we report the discovery of strong barred structures in massive disc galaxies at z ~ 1.5 in deep rest-frame optical images from the Cosmic Assembly Near-Infrared Deep Extragalactic Legacy Survey. From within a sample of 876 disc galaxies identified by visual classification in Galaxy Zoo, we identify 123 barred galaxies. Selecting a subsample within the same region of the evolving galaxy luminosity function (brighter than L*), we find that the bar fraction across the redshift range 0.5 ≤ z ≤ 2 (fbar = 10.7+6.3 -3.5 per cent after correcting for incompleteness) does not significantly evolve.We discuss the implications of this discovery in the context of existing simulations and our current understanding of the way disc galaxies have evolved over the last 11 billion yearsPeer reviewedFinal Accepted Versio
White matter microstructure associations to amyloid burden in adults with Down syndrome.
INTRODUCTION: Individuals with Down syndrome (DS) are at an increased risk of developing Alzheimer's Disease (AD). One of the early underlying mechanisms in AD pathology is the accumulation of amyloid protein plaques, which are deposited in extracellular gray matter and signify the first stage in the cascade of neurodegenerative events. AD-related neurodegeneration is also evidenced as microstructural changes in white matter. In this work, we explored the correlation of white matter microstructure with amyloid load to assess amyloid-related neurodegeneration in a cohort of adults with DS. METHODS: In this study of 96 adults with DS, the relation of white matter microstructure using diffusion tensor imaging (DTI) and amyloid plaque burden using [11C]PiB PET were examined. The amyloid load (AβL) derived from [11C]PiB was used as a global measure of amyloid burden. AβL and DTI measures were compared using tract-based spatial statistics (TBSS) and corrected for imaging site and chronological age. RESULTS: TBSS of the DTI maps showed widespread age-by-amyloid interaction with both fractional anisotropy (FA) and mean diffusivity (MD). Further, diffuse negative association of FA and positive association of MD with amyloid were observed. DISCUSSION: These findings are consistent with the white matter microstructural changes associated with AD disease progression in late onset AD in non-DS populations
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