642 research outputs found
A hydrogen beam to characterize the ASACUSA antihydrogen hyperfine spectrometer
The antihydrogen programme of the ASACUSA collaboration at the antiproton
decelerator of CERN focuses on Rabi-type measurements of the ground-state
hyperfine splitting of antihydrogen for a test of the combined
Charge-Parity-Time symmetry. The spectroscopy apparatus consists of a microwave
cavity to drive hyperfine transitions and a superconducting sextupole magnet
for quantum state analysis via Stern-Gerlach separation. However, the small
production rates of antihydrogen forestall comprehensive performance studies on
the spectroscopy apparatus. For this purpose a hydrogen source and detector
have been developed which in conjunction with ASACUSA's hyperfine spectroscopy
equipment form a complete Rabi experiment. We report on the formation of a
cooled, polarized, and time modulated beam of atomic hydrogen and its detection
using a quadrupole mass spectrometer and a lock-in amplification scheme. In
addition key features of ASACUSA's hyperfine spectroscopy apparatus are
discussed.
Evolution of the fishtail-effect in pure and Ag-doped MG-YBCO
We report on magnetic measurements carried out in a textured
YBaCuO and YBa(CuAg)O (at
0.02) crystals. The so-called fishtail-effect (FE) or second
magnetization peak has been observed in a wide temperature range
0.4~~0.8 for . The origin of the FE arises for
the competition between surface barrier and bulk pinning. This is confirmed in
a non-monotonically behavior of the relaxation rate . The value
for Ag-doped crystals is larger than for the pure one due to the presence of
additional pinning centers, above all on silver atoms.Comment: 6 pages, 6 figure
Identification of human pathogens isolated from blood using microarray hybridisation and signal pattern recognition
<p>Abstract</p> <p>Background</p> <p>Pathogen identification in clinical routine is based on the cultivation of microbes with subsequent morphological and physiological characterisation lasting at least 24 hours. However, early and accurate identification is a crucial requisite for fast and optimally targeted antimicrobial treatment. Molecular biology based techniques allow fast identification, however discrimination of very closely related species remains still difficult.</p> <p>Results</p> <p>A molecular approach is presented for the rapid identification of pathogens combining PCR amplification with microarray detection. The DNA chip comprises oligonucleotide capture probes for 25 different pathogens including Gram positive cocci, the most frequently encountered genera of <it>Enterobacteriaceae</it>, non-fermenter and clinical relevant <it>Candida </it>species. The observed detection limits varied from 10 cells (e.g. <it>E. coli</it>) to 10<sup>5 </sup>cells (<it>S. aureus</it>) per mL artificially spiked blood. Thus the current low sensitivity for some species still represents a barrier for clinical application. Successful discrimination of closely related species was achieved by a signal pattern recognition approach based on the k-nearest-neighbour method. A prototype software providing this statistical evaluation was developed, allowing correct identification in 100 % of the cases at the genus and in 96.7 % at the species level (n = 241).</p> <p>Conclusion</p> <p>The newly developed molecular assay can be carried out within 6 hours in a research laboratory from pathogen isolation to species identification. From our results we conclude that DNA microarrays can be a useful tool for rapid identification of closely related pathogens particularly when the protocols are adapted to the special clinical scenarios.</p
Characterization of a newly identified ETV6-NTRK3 fusion transcript in acute myeloid leukemia
BACKGROUND: Characterization of novel fusion genes in acute leukemia is important for gaining information about leukemia genesis. We describe the characterization of a new ETV6 fusion gene in acute myeloid leukemia (AML) FAB M0 as a result of an uncommon translocation involving chromosomes 12 and 15. METHODS: The ETV6 locus at 12p13 was shown to be translocated and to constitute the 5' end of the fusion product by ETV6 break apart fluorescence in situ hybridisation (FISH). To identify a fusion partner 3' rapid amplification of cDNA-ends with polymerase chain reaction (RACE PCR) was performed followed by cloning and sequencing. RESULTS: The NTRK3 gene on chromosome 15 was found to constitute the 3' end of the fusion gene and the underlying ETV6-NTRK3 rearrangement was verified by reverse transcriptase PCR. No RNA of the reciprocal NTRK3-ETV6 fusion gene could be detected. CONCLUSION: We have characterized a novel ETV6-NTRK3 fusion transcript which has not been previously described in AML FAB M0 by FISH and RACE PCR. ETV6-NTRK3 rearrangements have been described in secretory breast carcinoma and congenital fibrosarcoma
A Study of the PDGF Signaling Pathway with PRISM
In this paper, we apply the probabilistic model checker PRISM to the analysis
of a biological system -- the Platelet-Derived Growth Factor (PDGF) signaling
pathway, demonstrating in detail how this pathway can be analyzed in PRISM. We
show that quantitative verification can yield a better understanding of the
PDGF signaling pathway.Comment: In Proceedings CompMod 2011, arXiv:1109.104
Comprehensive dose evaluation of a Deep Learning based synthetic Computed Tomography algorithm for pelvic Magnetic Resonance-only radiotherapy
Sagopilone (ZK-EPO, ZK 219477) for recurrent glioblastoma. A phase II multicenter trial by the European Organisation for Research and Treatment of Cancer (EORTC) Brain Tumor Group
Background: Sagopilone (ZK 219477), a lipophylic and synthetic analog of epothilone B, that crosses the blood-brain barrier has demonstrated preclinical activity in glioma models. Patients and methods: Patients with first recurrence/progression of glioblastoma were eligible for this early phase II and pharmacokinetic study exploring single-agent sagopilone (16 mg/m2 over 3 h every 21 days). Primary end point was a composite of either tumor response or being alive and progression free at 6 months. Overall survival, toxicity and safety and pharmacokinetics were secondary end points. Results: Thirty-eight (evaluable 37) patients were included. Treatment was well tolerated, and neuropathy occurred in 46% patients [mild (grade 1) : 32%]. No objective responses were seen. The progression-free survival (PFS) rate at 6 months was 6.7% [95% confidence interval (CI) 1.3-18.7], the median PFS was just over 6 weeks, and the median overall survival was 7.6 months (95% CI 5.3-12.3), with a 1-year survival rate of 31.6% (95% CI 17.7-46.4). Maximum plasma concentrations were reached at the end of the 3-h infusion, with rapid declines within 30 min after termination. Conclusions: No evidence of relevant clinical antitumor activity against recurrent glioblastoma could be detected. Sagopilone was well tolerated, and moderate-to-severe peripheral neuropathy was observed in despite prolonged administratio
Background free search for neutrinoless double beta decay with GERDA Phase II
The Standard Model of particle physics cannot explain the dominance of matter
over anti-matter in our Universe. In many model extensions this is a very
natural consequence of neutrinos being their own anti-particles (Majorana
particles) which implies that a lepton number violating radioactive decay named
neutrinoless double beta () decay should exist. The detection
of this extremely rare hypothetical process requires utmost suppression of any
kind of backgrounds.
The GERDA collaboration searches for decay of Ge
(^{76}\rm{Ge} \rightarrow\,^{76}\rm{Se} + 2e^-) by operating bare detectors
made from germanium with enriched Ge fraction in liquid argon. Here, we
report on first data of GERDA Phase II. A background level of
cts/(keVkgyr) has been achieved which is the world-best if
weighted by the narrow energy-signal region of germanium detectors. Combining
Phase I and II data we find no signal and deduce a new lower limit for the
half-life of yr at 90 % C.L. Our sensitivity of
yr is competitive with the one of experiments with
significantly larger isotope mass.
GERDA is the first experiment that will be background-free
up to its design exposure. This progress relies on a novel active veto system,
the superior germanium detector energy resolution and the improved background
recognition of our new detectors. The unique discovery potential of an
essentially background-free search for decay motivates a
larger germanium experiment with higher sensitivity.Comment: 14 pages, 9 figures, 1 table; ; data, figures and images available at
http://www.mpi-hd.mpg/gerda/publi
Limits on uranium and thorium bulk content in GERDA Phase I detectors
Internal contaminations of U, U and Th in the bulk of
high purity germanium detectors are potential backgrounds for experiments
searching for neutrinoless double beta decay of Ge. The data from GERDA
Phase~I have been analyzed for alpha events from the decay chain of these
contaminations by looking for full decay chains and for time correlations
between successive decays in the same detector. No candidate events for a full
chain have been found. Upper limits on the activities in the range of a few
nBq/kg for Ra, Ac and Th, the long-lived daughter
nuclides of U, U and Th, respectively, have been
derived. With these upper limits a background index in the energy region of
interest from Ra and Th contamination is estimated which
satisfies the prerequisites of a future ton scale germanium double beta decay
experiment.Comment: 2 figures, 7 page
The first search for bosonic super-WIMPs with masses up to 1 MeV/c with GERDA
We present the first search for bosonic super-WIMPs as keV-scale dark matter
candidates performed with the GERDA experiment. GERDA is a neutrinoless
double-beta decay experiment which operates high-purity germanium detectors
enriched in Ge in an ultra-low background environment at the Laboratori
Nazionali del Gran Sasso (LNGS) of INFN in Italy. Searches were performed for
pseudoscalar and vector particles in the mass region from 60 keV/c to 1
MeV/c. No evidence for a dark matter signal was observed, and the most
stringent constraints on the couplings of super-WIMPs with masses above 120
keV/c have been set. As an example, at a mass of 150 keV/c the most
stringent direct limits on the dimensionless couplings of axion-like particles
and dark photons to electrons of and
at 90% credible interval,
respectively, were obtained.Comment: 6 pages, 3 figures, submitted to Physical Review Letters, added list
of authors, updated ref. [21
- …