Development of a Pre-Driven Recovery Evaluation Program for Longwall Operations

Many longwall coordinators are examining the use of pre-driven recovery roadways. This method, if performed successfully can improve the overall efficiency and safety of moving longwall equipment from panel to panel. However, it is difficult to assess the feasibility of using predriven recovery unless extensive research is carried out or a consultant is used to analyse the particular situation. A number of previous case studies have been analysed to discover which parameters have the greatest influence on the success of pre-driven recovery. Floor strength, Coal Mine Roof Rating (CMRR), extraction depth, Roof Density Index (RDI), standing support and mining rate were the main parameters impacting on the successful implementation of pre-driven recovery roadways. These parameters have been incorporated into a program that was developed to assess the feasibility of using pre-driven recovery roadways. The Pre-driven Recovery Evaluation Program (PREP) is simple to operate and it will enable new longwall mining operations as well as current operations to quickly determine the suitability of the method to their site

INEEL Subregional Conceptual Model Report Volume 3: Summary of Existing Knowledge of Natural and Anthropogenic Influences on the Release of Contaminants to the Subsurface Environment from Waste Source Terms at the INEEL

This source-term summary document is intended to describe the current understanding of contaminant source terms and the conceptual model for potential source-term release to the environment at the Idaho National Engineering and Environmental Laboratory (INEEL), as presented in published INEEL reports. The document presents a generalized conceptual model of the sources of contamination and describes the general categories of source terms, primary waste forms, and factors that affect the release of contaminants from the waste form into the vadose zone and Snake River Plain Aquifer. Where the information has previously been published and is readily available, summaries of the inventory of contaminants are also included. Uncertainties that affect the estimation of the source term release are also discussed where they have been identified by the Source Term Technical Advisory Group. Areas in which additional information are needed (i.e., research needs) are also identified

Development of probing strategies to investigate metabolic flux of biofuel production pathways in Clostridia

Currently, fossil fuels contribute a large number of high value chemicals that are used on a daily basis. Crude oil is cracked to give a number of high value chemicals, including vehicle fuels as well as chemicals and solvents that are used daily both commercially and industrially. However, fossil fuel reserves are in decline, with research going into alternatives to obtain these useful chemicals, one of which is biofuels. Biofuels can be generated in a number of ways, one of which is the fermentation of acetogenic bacteria, microorganisms that generate acetate as a product of anaerobic metabolism. Clostridium autoethanogenum is an acetogen that can grow on one carbon gases as its feedstock, and can be used to generate valuable chemicals, with scope to develop the range of metabolic products further. One aim of this project was to investigate the metabolic flux through pathways of the bacterium using isotopically labelled compounds, which would be assessed by mass spectrometry and NMR. Following on from this, design of inhibitors for the enzymes of the pathways with a view to drive the metabolic processes towards higher value chemical compounds by ‘switching off’ other branches of the pathway. Putative small molecule mimics of acetyl-CoA, SNAC thioesters, were synthesised and tested for uptake and activity in whole cell growth experiments with C. autoethanogenum, and determined to be unsuccessful. Further to this, compounds were designed and synthesised to replace pantothenic acid in the growth media, which were not tested in growth experiments. A library of inhibitor compounds was synthesised and tested against recombinantly purified acetate kinase. A number of compounds were shown to inhibit the enzyme, and the mode of inhibition was determined, as well as IC50 and Ki values for each. This project operated as part of a larger GASCHEM project in the Synthetic Biology Research Centre at the University of Nottingham

Generating Instructions at Different Levels of Abstraction

When generating technical instructions, it is often convenient to describe complex objects in the world at different levels of abstraction. A novice user might need an object explained piece by piece, while for an expert, talking about the complex object (e.g. a wall or railing) directly may be more succinct and efficient. We show how to generate building instructions at different levels of abstraction in Minecraft. We introduce the use of hierarchical planning to this end, a method from AI planning which can capture the structure of complex objects neatly. A crowdsourcing evaluation shows that the choice of abstraction level matters to users, and that an abstraction strategy which balances low-level and high-level object descriptions compares favorably to ones which don't.Comment: Accepted COLING 2020 long pape

Energy - Water Nexus -- Meeting the Energy and Water Needs of the Snake/Columbia River Basin in the 21st CenturyScience and Technology SummitConference Results

In June 2007, representatives from federal, state, and academic institutions met to discuss the role of innovative science, technology, and policy in meeting future energy and water demands in the Snake-Columbia River Basin. Conference members assessed the state-of-the-science, technology, and associated research to develop cost-effective and environmentally sound methodologies and technologies to maximize the production of energy and availability of water and to minimize the consumption of both water and energy in the Snake-Columbia River system. Information on all phases of science and technology development, theoretical analysis, laboratory experiments, pilot tests, and field applications were relevant topics for discussion. An overview of current management needs was presented the first day. On the second day, five focus groups were created: ? Energy Generation and Use ? Water Allocation and Use ? Energy/Water Storage ? Environmental Considerations ? Social, Economic, Political, and Regulatory Considerations. Each group started with a list of status items and trends, and discussed the future challenges and research needed to reach four goals: ? Balance energy production and resource consumption ? Balance water availability and competing needs ? Balance water consumption/energy production and competing needs ? Balance environmental impacts and water use/energy production ? Balance costs and benefits of water use. The resulting initiatives were further broken down into three categories of importance: critical, important, and nice to do but could be delayed. Each initiative was assigned a number of dots to show a more refined ranking. The results of each focus group are given in the pages that follow. These results are intended to help local and regional researchers 1. Develop a technical strategy for developing cost-effective science and technology to predict, measure, monitor, purify, conserve, and store water and to maximize power generation, storage, and efficiency in the region 2. Evaluate methods and technologies for reducing the impacts of energy and water development and use on the environment

INEEL Subregional Conceptual Model Report; Volume 1 - Summary of Existing Knowledge of Natural and Anthropogenic Influences Governing Subsurface Contaminant Transport in the INEEL Subregion of the Eastern Snake River Plain

The National Research Council has defined a conceptual model as ''an evolving hypothesis identifying the important features, processes, and events controlling fluid flow and contaminant transport of consequence at a specific field site in the context of a recognized problem''. Presently, several subregional conceptual models are under development at the Idaho National Engineering and Environmental Laboratory (INEEL). Additionally, facility-specific conceptual models have been described as part of INEEL environmental restoration activities. Compilation of these models is required to develop a comprehensive conceptual model that can be used to strategically plan for future groundwater research activities at the INEEL. Conceptual models of groundwater flow and contaminant transport at the INEEL include the description of the geologic framework, matrix hydraulic properties, and inflows and outflows. They also include definitions of the contaminant source term and contaminant transport mechanisms. The geologic framework of the INEEL subregion is described by the geometry of the system, stratigraphic units within the system, and structural features that affect groundwater flow and contaminant transport. These elements define geohydrologic units that make up the Snake River Plain Aquifer (SRPA). The United States Geological Survey (USGS) conceptual model encompasses approximately 1,920 mi2 of the eastern Snake River Plain. The Waste Area Group (WAG)-10 model includes the USGS area and additional areas to the northeast and southeast. Both conceptual models are bounded to the northwest by the Pioneer Mountains, Lost River Range, and Lemhi Mountains. They are bounded to the southeast by groundwater flow paths determined from aquifer water-level contours. The upgradient extent of the USGS model is a water-level contour that includes the northeastern boundary of the INEEL. The WAG-10 model includes more of the Mud Lake area to utilize previous estimates of underflow into the subregion. Both conceptual models extend approximately 25 miles to the southwest of the INEEL, a distance sufficient to include known concentrations of contaminant tracers. Several hypotheses have been developed concerning the effective thickness of the SRPA at the INEEL. The USGS model has defined the effective thickness from electrical resistivity and borehole data to be as much as 2,500 ft in the eastern part of the subregion and as much as 4,000 ft in the southwestern part. The WAG-10 model has developed two alternatives using aquifer-temperature and electrical resistivity data. The ''thick'' aquifer interpretation utilizes colder temperature data and includes a northtrending zone in which the thickness exceeds 1,300 ft and with a maximum thickness of 1,700 ft. The ''thin'' aquifer interpretation minimizes aquifer thickness, with thickness ranging from 328 to 1,300 ft. Facility-specific models generally have focused efforts on the upper 250 ft of saturation. Conceptual models have utilized a stratigraphic data set to define geohydrologic units within the INEEL subregion. This data set, compiled from geophysical logs and cores from boreholes, correlates the thick, complex stack of basalt flows across the subregion. Conceptual models generally concur that the upper geohydrologic unit consists of a section of highly fractured, multiple, thin basalt flows and sedimentary interbeds. Beneath this unit is an areally extensive, thick, unfractured basalt flow that rises above the water table southwest of the INEEL. The bottom unit consists of a thick section of slightly- to moderately-altered basalt. A key objective of the DOE water-integration project at the INEEL is to coordinate development of a subregional conceptual model of groundwater flow and contaminant transport that is based on the best available understanding of geologic and hydrologic features. The first step in this process is to compile and summarize the current conceptual models of groundwater flow and contaminant transport at the INEEL that have been developed from extensive geohydrologic studies conducted during the last 50 years

INEEL Subregional Conceptual Model Report Volume 2: Summary of Existing Knowledge of Geochemical Influences on the Fate and Transport of Contaminants in the Subsurface at the INEEL

This document summarizes previous descriptions of geochemical system conceptual models for the vadose zone and groundwater zone (aquifer) beneath the Idaho National Engineering and Environmental Laboratory (INEEL). The primary focus is on groundwater because contaminants derived from wastes disposed at INEEL are present in groundwater, groundwater provides a pathway for potential migration to receptors, and because geochemical characteristics in and processes in the aquifer can substantially affect the movement, attenuation, and toxicity of contaminants. The secondary emphasis is perched water bodies in the vadose zone. Perched water eventually reaches the regional groundwater system, and thus processes that affect contaminants in the perched water bodies are important relative to the migration of contaminants into groundwater. Similarly, processes that affect solutes during transport from nearsurface disposal facilities downward through the vadose zone to the aquifer are relevant. Sediments in the vadose zone can affect both water and solute transport by restricting the downward migration of water sufficiently that a perched water body forms, and by retarding solute migration via ion exchange. Geochemical conceptual models have been prepared by a variety of researchers for different purposes. They have been published in documents prepared by INEEL contractors, the United States Geological Survey (USGS), academic researchers, and others. The documents themselves are INEEL and USGS reports, and articles in technical journals. The documents reviewed were selected from citation lists generated by searching the INEEL Technical Library, the INEEL Environmental Restoration Optical Imaging System, and the ISI Web of Science databases. The citation lists were generated using the keywords ground water, groundwater, chemistry, geochemistry, contaminant, INEL, INEEL, and Idaho. In addition, a list of USGS documents that pertain to the INEEL was obtained and manually searched. The documents that appeared to be the most pertinent were selected from further review. These documents are tabulated in the citation list. This report summarizes existing geochemical conceptual models, but does not attempt to generate a new conceptual model or select the ''right'' model. This document is organized as follows. Geochemical models are described in general in Section 2. Geochemical processes that control the transport and fate of contaminants introduced into groundwater are described in Section 3. The natural geochemistry of the Eastern Snake River Plain Aquifer (SRPA) is described in Section 4. The effect of waste disposal on the INEEL subsurface is described in Section 5. The geochemical behavior of the major contaminants is described in Section 6. Section 7 describes the site-specific geochemical models developed for various INEEL facilities

An RNA toolbox for single-molecule force spectroscopy studies

Precise, controllable single-molecule force spectroscopy studies of RNA and RNA-dependent processes have recently shed new light on the dynamics and pathways of RNA folding and RNA-enzyme interactions. A crucial component of this research is the design and assembly of an appropriate RNA construct. Such a construct is typically subject to several criteria. First, single-molecule force spectroscopy techniques often require an RNA construct that is longer than the RNA molecules used for bulk biochemical studies. Next, the incorporation of modified nucleotides into the RNA construct is required for its surface immobilization. In addition, RNA constructs for single-molecule studies are commonly assembled from different single-stranded RNA molecules, demanding good control of hybridization or ligation. Finally, precautions to prevent RNase- and divalent cation-dependent RNA digestion must be taken. The rather limited selection of molecular biology tools adapted to the manipulation of RNA molecules, as well as the sensitivity of RNA to degradation, make RNA construct preparation a challenging task. We briefly illustrate the types of single-molecule force spectroscopy experiments that can be performed on RNA, and then present an overview of the toolkit of molecular biology techniques at one's disposal for the assembly of such RNA constructs. Within this context, we evaluate the molecular biology protocols in terms of their effectiveness in producing long and stable RNA constructs

Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium

Clostridium autoethanogenum is an acetogenic bacterium capable of producing high value commodity chemicals and biofuels from the C1 gases present in synthesis gas. This common industrial waste gas can act as the sole energy and carbon source for the bacterium that converts the low value gaseous components into cellular building blocks and industrially relevant products via the action of the reductive acetyl-CoA (Wood-Ljungdahl) pathway. Current research efforts are focused on the enhancement and extension of product formation in this organism via synthetic biology approaches. However, crucial to metabolic modelling and directed pathway engineering is a reliable and comprehensively annotated genome sequence