3,881 research outputs found
Small GTPase Rap1 Is Essential for Mouse Development and Formation of Functional Vasculature
BACKGROUND: Small GTPase Rap1 has been implicated in a number of basic cellular functions, including cell-cell and cell-matrix adhesion, proliferation and regulation of polarity. Evolutionarily conserved, Rap1 has been studied in model organisms: yeast, Drosophila and mice. Mouse in vivo studies implicate Rap1 in the control of multiple stem cell, leukocyte and vascular cell functions. In vitro, several Rap1 effectors and regulatory mechanisms have been proposed. In particular, Rap1 has been implicated in maintaining epithelial and endothelial cell junction integrity and linked with cerebral cavernous malformations.
RATIONALE: How Rap1 signaling network controls mammalian development is not clear. As a first step in addressing this question, we present phenotypes of murine total and vascular-specific Rap1a, Rap1b and double Rap1a and Rap1b (Rap1) knockout (KO) mice.
RESULTS AND CONCLUSIONS: The majority of total Rap1 KO mice die before E10.5, consistent with the critical role of Rap1 in epithelial morphogenesis. At that time point, about 50% of Tie2-double Rap1 KOs appear grossly normal and develop normal vasculature, while the remaining 50% suffer tissue degeneration and show vascular abnormalities, including hemorrhages and engorgement of perineural vessels, albeit with normal branchial arches. However, no Tie2-double Rap1 KO embryos are present at E15.5, with hemorrhages a likely cause of death. Therefore, at least one Rap1 allele is required for development prior to the formation of the vascular system; and in endothelium-for the life-supporting function of the vasculature
Teacher’s Appropriate Humor and Its Impacts on English Classroom Environment at English Course
This research investigates teacher’s humor as a communication strategy in terms of interaction between the teacher and the students in teaching and learning process at Makassar English Course. The aims of the study are (1) to find out the types of humor that are appropriate in English course and (2) to find out the impacts of teacher’s humor at English course.
This research consisted of one teacher that was observed and eight students that were interviewed as the subject of the research. Five meetings of classroom interactions were observed and an video-recorder was used by the researcher. The recordings were transcribed and analyzed by making use of related the types of humor are used by the teacher and the impacts of teacher’s humor gained from students’ perspective at Makassar English course.
Findings show that there are some types of humors that are appropriate to be used by the teacher namely related humor, unrelated humor, self-disparaging, and unplanned humor. Furthermore, the finding also showed that the teacher who used humor makes the students easy to getting the knowledge, reduced tension, made students more comfortable in teaching learning process, created good interactions, created more enthusiastic teaching learning process, encouraged students’ attendance, and reduced the number of students who felt sleepy in the class.
Keywords: EFL teacher, appropriate humor, impact of humors, English cours
Expression and Function of Calcium Binding Domain Chimeras of the Integrins α IIb and α 5
To further identify amino acid domains involved in the ligand binding specificity of alpha(IIb)beta(3), chimeras of the conserved calcium binding domains of alpha(IIb) and the alpha subunit of the fibronectin receptor alpha(5)beta(1) were constructed. Chimeras that replaced all four calcium binding domains, replaced all but the second calcium binding domain of alpha(IIb) with those of alpha(5), or deleted all four calcium binding domains were synthesized but not expressed on the cell surface. Additional chimeras exchanged subsets or all of the variant amino acids in the second calcium binding domain, a region implicated in ligand binding. Cell surface expression of each second calcium binding domain mutant complexed with beta(3) was observed. Each second calcium binding domain mutant was able to 1) bind to immobilized fibrinogen, 2) form fibrinogen-dependent aggregates after treatment with dithiothreitol, and 3) bind the activation-dependent antibody PAC1 after LIBS 6 treatment. Soluble fibrinogen binding studies suggested that there were only small changes in either the K(d) or B(max) of any mutant. We conclude that chimeras of alpha(IIb) containing the second calcium binding domain sequences of alpha(5) are capable of complexing with beta(3), that the complexes are expressed on the cell surface, and that mutant complexes are capable of binding both immobilized and soluble fibrinogen, suggesting that the second calcium binding domain does not determine ligand binding specificity
The SPLASH Survey: A Spectroscopic Portrait of Andromeda's Giant Southern Stream
The giant southern stream (GSS) is the most prominent tidal debris feature in
M31's stellar halo. The GSS is composed of a relatively metal-rich, high
surface-brightness "core" and a lower metallicity, lower surface brightness
"envelope." We present Keck/DEIMOS spectroscopy of red giant stars in six
fields in the vicinity of M31's GSS and one field on Stream C, an arc-like
feature on M31's SE minor axis at R=60 kpc. Several GSS-related findings and
measurements are presented here. We present the innermost kinematical detection
of the GSS core to date (R=17 kpc). This field also contains the continuation
of a second kinematically cold component originally seen in a GSS core field at
R=21 kpc. The velocity gradients of the GSS and the second component in the
combined data set are parallel over a radial range of 7 kpc, suggesting a
possible bifurcation in the line-of-sight velocities of GSS stars. We also
present the first kinematical detection of substructure in the GSS envelope.
Using kinematically identified samples, we show that the envelope debris has a
~0.7 dex lower mean photometric metallicity and possibly higher intrinsic
velocity dispersion than the GSS core. The GSS is also identified in the field
of the M31 dSph satellite And I; the GSS in this field has a metallicity
distribution identical to that of the GSS core. We confirm the presence of two
kinematically cold components in Stream C, and measure intrinsic velocity
dispersions of ~10 and ~4 km/s. This compilation of the kinematical (mean
velocity, intrinsic velocity dispersion) and chemical properties of stars in
the GSS core and envelope, coupled with published surface brightness
measurements and wide-area star-count maps, will improve constraints on the
orbit and internal structure of the dwarf satellite progenitor.Comment: Accepted for publication in Ap
Stellar Kinematics in the Complicated Inner Spheroid of M31: Discovery of Substructure Along the Southeastern Minor Axis and its Relationship to the Giant Southern Stream
We present the discovery of a kinematically-cold stellar population along the
SE minor axis of the Andromeda galaxy (M31) that is likely the forward
continuation of M31's giant southern stream. This discovery was made in the
course of an on-going spectroscopic survey of red giant branch (RGB) stars in
M31 using the DEIMOS instrument on the Keck II 10-m telescope. Stellar
kinematics are investigated in eight fields located 9-30 kpc from M31's center
(in projection). A likelihood method based on photometric and spectroscopic
diagnostics is used to isolate confirmed M31 RGB stars from foreground Milky
Way dwarf stars: for the first time, this is done without using radial velocity
as a selection criterion, allowing an unbiased study of M31's stellar
kinematics. The radial velocity distribution of the 1013 M31 RGB stars shows
evidence for the presence of two components. The broad (hot) component has a
velocity dispersion of 129 km/s and presumably represents M31's virialized
spheroid. A significant fraction (19%) of the population is in a narrow (cold)
component centered near M31's systemic velocity with a velocity dispersion that
decreases with increasing radial distance, from 55.5 km/s at R_proj=12 kpc to
10.6 km/s at R_proj=18 kpc. The spatial and velocity distribution of the cold
component matches that of the "Southeast shelf" predicted by the Fardal et al.
(2007) orbital model of the progenitor of the giant southern stream. The
metallicity distribution of the cold component matches that of the giant
southern stream, but is about 0.2 dex more metal rich on average than that of
the hot spheroidal component. We discuss the implications of our discovery on
the interpretation of the intermediate-age spheroid population found in this
region in recent ultra-deep HST imaging studies.Comment: 23 pages, 16 figures, 2 tables, accepted for publication in the
Astrophysical Journal. Changes from previous version: expanded discussion in
sections 4.2 and 7.2, removal of section 7.1.4 and associated figure
(discussion moved to section 7.1.2
Ongoing challenges in the management of malaria
This article gives an overview of some of the ongoing challenges that are faced in the prevention, diagnosis and treatment of malaria
Level density and gamma strength function in 162-Dy from inelastic 3-He scattering
Complementary measurements have been performed for the level density and
gamma strength function in 162-Dy using inelastic 3-He scattering. Comparing
these results to previous measurements using the 163-Dy(3-He,alpha) reaction,
reveals that the measured quantities above 1.5 MeV do not depend significantly
on the nuclear reaction chosen.Comment: 15 pages, including 7 figure
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