Cultural Resources Monitoring Investigations for the San Antonio Water Systems (SAWS) Silverhorn Rehabilitation Project San Antonio, Bexar County, Texas

Raba Kistner, Inc. (RKI), was contracted by K Friese + Associates (CLIENT) to conduct cultural resources monitoring investigations for the San Antonio Water Systems (SAWS) Silverhorn Rehabilitation Project in northern San Antonio, Bexar County, Texas. The project involved rehabilitating 3,690 linear feet of existing sewer line within the Silverhorn Golf Club, which is within the Walker Ranch National Register District. The majority of the proposed undertaking was conducted by non-invasive methods (i.e., cured-in-place-pipe (CIPP)); however, point repairs involving excavations were conducted in areas where broken pipe was encountered. Given that the proposed undertaking is located on lands owned by the City of San Antonio (COSA), and because COSA is a political subdivision of the State of Texas, the project falls under the jurisdiction of Chapter 35 of the Unified Development Code (UDC) of the City of San Antonio, as well as the Antiquities Code of Texas (ACT). The UDC is regulated by the COSA Office of Historic Preservation (OHP), while the ACT is administered by the Texas Historical Commission (THC). Cultural resources investigations for the project were conducted on January 11, 12, and 24, 2020. Jason M. Whitaker served as Principal Investigator and Project Manager for the duration of the project, and all fieldwork was completed by Jason M. Whitaker and Christopher Matthews. All work was conducted in accordance with the standards set forth by the Council of Texas Archeologists and adopted by the Texas Historical Commission, under Texas Antiquities Committee Permit Number 9220. The overall undertaking consisted of the rehabilitation of approximately 3,690 linear feet of existing sewer line within the Silverhorn Golf Club. The majority of the project area was rehabilitated through the use of cured-in-place-pipe (CIPP) with no subsurface impacts. However, four areas along the 3,960 sewer line alignment were in need of point repairs which required subsurface excavations. For archaeological purposes, the Area of Potential Effects (APE) was comprised of four areas where subsurface excavations were conducted. Excavation within the APE areas were comprised of a single backhoe trench measuring approximately 22.3 by 13.1 feet (6.8 meters [m] by 4.0 m) and three 5-inch diameter boreholes. The combined area of impact of the APE areas measured approximately 0.007 acres. Depths of impacts reached 10.33 feet below surface within the trench, and a depth of 5.5 feet below surface. Disturbances within the APE included the existing sewer line, golf course construction, and various associated utilities. The APE was located entirely within areas impacted by the installation of the existing sewer line and was evident during the monitoring of the excavations. Evidence of previous disturbances was observed during the monitoring of the point repairs. No cultural deposits or features were documented during monitoring excavations of the SAWS Silverhorn Rehabilitation Project. Additionally, no evidence of unmarked burials or human remains were observed. Given that excavations occurred over a previously excavated areas and no cultural materials were identified, RKI recommends no further archaeological investigations for the current APE. However, should additional point repairs along the existing sewer line be required, it is recommended that additional monitoring be conducted. All field records generated by this project will be permanently curated at the Center for Archaeological Research at the University of Texas at San Antonio

Cultural Resources Investigations of the Brooks City Base Proposed Improvements Street And Drainage Project, San Antonio, Bexar County, Texas

Raba Kistner Environmental, Inc. (RKEI), was contracted by the Brooks Development Authority (CLIENT) to conduct an intensive cultural resources survey of 34.95 acres at the southern boundary of Brooks City Base between the southern terminus of South New Braunfels Avenue and the western terminus of Lyster Road. This survey was in advance of the connection of South New Braunfels Avenue with Lyster Road, the improvement of 3,500 linear feet of the existing Lyster Road right-of-way, the installation of utility lines (telecommunications, underground electric, potable and recycled water), as well as surface grading, installation of culverts, and the construction of a larger detention basin for storm water management. Working cooperatively with the CLIENT, this project was funded by the City of San Antonio (COSA) through the 2017 Bond Program. As such, the project fell under the jurisdiction of Chapter 35 of the COSA Unified Development Code (UDC), as well as the Antiquities Code of Texas (ACT) (Texas Natural Resource Code, Title 9, Chapter 191), by virtue of it representing a public undertaking. Furthermore, the undertaking will required a Preconstruction Notification under Nationwide Permit 43, Stormwater Management Facilities. As such, the project also fell under the jurisdiction of Section 106 of the National Historic Preservation Act (NHPA) (16 United States Code 470) and it’s implementing regulations (36 Code of Federal Regulation 800). On September 19 and 20, 2018, RKEI archaeologists conducted an intensive pedestrian survey augmented with both shovel testing and backhoe trenching for the 34.95 acres of Brooks City Base associated with this project. Antonio E. Padilla, M.A. served as Principal Investigator and all field work was conducted by Archaeologists Jason M. Whitaker and Kirsten M. Atwood. A total of 19 shovel tests were excavated, as well as two backhoe trenches within the project area: five shovel tests (ST-13-18) and two backhoe trenches (BHT 1-2) in the southwest section of the APE, one in the southeastern section (ST-12) a planned shovel test (ST-18) was not excavated due to standing water, five in the northeast section (ST-6-9 and 10) a planned shovel test (ST-10) was not excavated due to standing water, and eight in the northwest section (ST-1-5 and ST-19-21). Asphalt fragments were observed in several of these shovel tests (ST-4, 16, 19, and 21), which were most likely fragments from utility roads associated with the former Brooks Air Force Base and were not considered culturally significant. As such, none of the shovel tests or backhoe trenches were positive for subsurface cultural materials. The pedestrian survey did, however, document numerous above ground structures associated with the former Brooks Air Force Base FamCamp and other associated camping facilities. Overall, no significant prehistoric or historic materials or features were encountered within the Area of Potential Effects (APE). Given this conclusion, no significant cultural deposits will be impacted by the proposed project, and RKEI recommends no further archaeological investigations for the current APE. However, should additions be made to the project area, it is recommended that additional testing be conducted to determine the extent and significance of cultural deposits beyond the currently defined boundaries. All field records generated by this project will be permanently curated at the University of Texas at San Antonio Center for Archaeological Research (UTSA-CAR)

Blunt Abdominal Trauma Patients Are at Very Low Risk for Intra-Abdominal Injury after Emergency Department Observation

<p>Introduction: Patients are commonly admitted to the hospital for observation following blunt abdominal trauma (BAT), despite initially negative emergency department (ED) evaluations. With the current use of screening technology, such as computed tomography (CT) of the abdomen and pelvis, ultrasound, and laboratory evaluations, it is unclear which patients require observation. The objective of this study was to determine the prevalence of intra-abdominal injury (IAI) and death in hemodynamically normal and stable BAT patients with initially negative ED evaluations admitted to an ED observation unit and to define a low-risk subgroup of patients and assess whether they may be discharged without abdominal/pelvic CT or observation. Methods: This was a retrospective cohort study performed at an urban level 1 trauma center and included all BAT patients admitted to an ED observation unit as part of a BAT key clinical pathway. All were observed for at least 8 hours as part of the key clinical pathway, and only minors and pregnant women were excluded. Outcomes included the presence of IAI or death during a 40-month follow-up period. Prior to data collection, low-risk criteria were defined as no intoxication, no hypotension or tachycardia, no abdominal pain or tenderness, no hematuria, and no distracting injury. To be considered low risk, patients needed to meet all low-risk criteria. Results: Of the 1,169 patients included over the 2-year study period, 29% received a CT of the abdomen and pelvis, 6% were admitted to the hospital from the observation unit for further management, 0.4% (95% confidence interval [CI], 0.1%–1%) were diagnosed with IAI, and 0% (95% CI, 0%–0.3%) died. Patients had a median combined ED and observation length of stay of 9.5 hours. Of the 237 (20%) patients who met low-risk criteria, 7% had a CT of the abdomen and pelvis and 0% (95% CI, 0%–1.5%) were diagnosed with IAI or died. Conclusion: Most BAT patients who have initially negative ED evaluations are at low risk for IAI but still require some combination of observation and CT. A subgroup of BAT patients may be safely discharged without CT or observation after the initial evaluation. [West J Emerg Med. 2011;12(4):496–504.]</p

MCT1 in Invasive Ductal Carcinoma: Monocarboxylate Metabolism and Aggressive Breast Cancer.

Introduction: Monocarboxylate transporter 1 (MCT1) is an importer of monocarboxylates such as lactate and pyruvate and a marker of mitochondrial metabolism. MCT1 is highly expressed in a subgroup of cancer cells to allow for catabolite uptake from the tumor microenvironment to support mitochondrial metabolism. We studied the protein expression of MCT1 in a broad group of breast invasive ductal carcinoma specimens to determine its association with breast cancer subtypes and outcomes. Methods: MCT1 expression was evaluated by immunohistochemistry on tissue micro-arrays (TMA) obtained through our tumor bank. Two hundred and fifty-seven cases were analyzed: 180 cases were estrogen receptor and/or progesterone receptor positive (ER+ and/or PR+), 62 cases were human epidermal growth factor receptor 2 positive (HER2+), and 56 cases were triple negative breast cancers (TNBC). MCT1 expression was quantified by digital pathology with Aperio software. The intensity of the staining was measured on a continuous scale (0-black to 255-bright white) using a co-localization algorithm. Statistical analysis was performed using a linear mixed model. Results: High MCT1 expression was more commonly found in TNBC compared to ER+ and/or PR+ and compared to HER-2+ (p \u3c 0.001). Tumors with an in-situ component were less likely to stain strongly for MCT1 (p \u3c 0.05). High nuclear grade was associated with higher MCT1 staining (p \u3c 0.01). Higher T stage tumors were noted to have a higher expression of MCT1 (p \u3c 0.05). High MCT1 staining in cancer cells was associated with shorter progression free survival, increased risk of recurrence, and larger size independent of TNBC status (p \u3c 0.05). Conclusion: MCT1 expression, which is a marker of high catabolite uptake and mitochondrial metabolism, is associated with recurrence in breast invasive ductal carcinoma. MCT1 expression as quantified with digital image analysis may be useful as a prognostic biomarker and to design clinical trials using MCT1 inhibitors

Orbital atomic oxygen effects on materials: An overview of MSFC experiments on the STS-46 EOIM-3

The third Evaluation of Oxygen Interaction with Materials experiment was flown on Space Shuttle Mission STS-46 (July 31 - August 8, 1992), representing a joint effort of several NASA centers, universities, and contractors. This array of active instrumentation and material exposure sub-assemblies was integrated as a Shuttle cargo bay pallet experiment for investigating the effects of orbital atomic oxygen on candidate space materials. Marshall Space Flight Center contributed several passive exposure trays of material specimens, uniform stress and static stress material exposure fixtures, the Atomic Oxygen Resistance Monitor (AORM), and specimens of thermal coatings for the EOIM-3 variable exposure mechanisms. As a result of 42 hours of spacecraft velocity vector-oriented exposure during the later phases of the STS-46 mission in LEO, EOIM-3 materials were exposed to an atomic oxygen fluence of 2.2 x 10(exp 20) atoms/sq cm. In this paper, an overview is presented of the technical approaches and results from analyses of the MSFC flight specimens, fixtures, and the AORM. More detailed results from earlier EOIM missions, the LDEF, and from laboratory testing are included in associated papers of this conference session

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

4. TITLE AND SUBTITLE F/A-18(A-D) Wing Root Fatigue Life Expended (FLE)

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instruction, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, t

Blunt Abdominal Trauma Patients Are at Very Low Risk for Intra-Abdominal Injury after Emergency Department Observation

Introduction: Patients are commonly admitted to the hospital for observation following blunt abdominal trauma (BAT), despite initially negative emergency department (ED) evaluations. With the current use of screening technology, such as computed tomography (CT) of the abdomen and pelvis, ultrasound, and laboratory evaluations, it is unclear which patients require observation. The objective of this study was to determine the prevalence of intra-abdominal injury (IAI) and death in hemodynamically normal and stable BAT patients with initially negative ED evaluations admitted to an ED observation unit and to define a low-risk subgroup of patients and assess whether they may be discharged without abdominal/pelvic CT or observation. Methods: This was a retrospective cohort study performed at an urban level 1 trauma center and included all BAT patients admitted to an ED observation unit as part of a BAT key clinical pathway. All were observed for at least 8 hours as part of the key clinical pathway, and only minors and pregnant women were excluded. Outcomes included the presence of IAI or death during a 40-month follow-up period. Prior to data collection, low-risk criteria were defined as no intoxication, no hypotension or tachycardia, no abdominal pain or tenderness, no hematuria, and no distracting injury. To be considered low risk, patients needed to meet all low-risk criteria. Results: Of the 1,169 patients included over the 2-year study period, 29% received a CT of the abdomen and pelvis, 6% were admitted to the hospital from the observation unit for further management, 0.4% (95% confidence interval [CI], 0.1%–1%) were diagnosed with IAI, and 0% (95% CI, 0%–0.3%) died. Patients had a median combined ED and observation length of stay of 9.5 hours. Of the 237 (20%) patients who met low-risk criteria, 7% had a CT of the abdomen and pelvis and 0% (95% CI, 0%–1.5%) were diagnosed with IAI or died. Conclusion: Most BAT patients who have initially negative ED evaluations are at low risk for IAI but still require some combination of observation and CT. A subgroup of BAT patients may be safely discharged without CT or observation after the initial evaluation. [West J Emerg Med. 2011;12(4):496–504.

Infection with Bacteroides Phage BV01 Alters the Host Transcriptome and Bile Acid Metabolism in a Common Human Gut Microbe.

Gut-associated phages are hypothesized to alter the abundance and activity of their bacterial hosts, contributing to human health and disease. Although temperate phages constitute a significant fraction of the gut virome, the effects of lysogenic infection are underexplored. We report that the temperate phage, Bacteroides phage BV01, broadly alters its host's transcriptome, the prominent human gut symbiont Bacteroides vulgatus. This alteration occurs through phage-induced repression of a tryptophan-rich sensory protein (TspO) and represses bile acid deconjugation. Because microbially modified bile acids are important signals for the mammalian host, this is a mechanism by which a phage may influence mammalian phenotypes. Furthermore, BV01 and its relatives in the proposed phage family Salyersviridae are ubiquitous in human gut metagenomes, infecting a broad range of Bacteroides hosts. These results demonstrate the complexity of phage-bacteria-mammal relationships and emphasize a need to better understand the role of temperate phages in the gut microbiome