Human-wildlife interactions in conservation translocations: Developing guidelines

Workshop: Reintroductions and rewilding can be powerful tools in biodiversity recovery. We will introduce key Human-Wildlife Interaction issues that take place along the life cycle of a conservation translocation project, from planning to post-exit stages. Participants will be invited to discuss their experiences in HWI related to each of these stages, aiming to expand on findings from the Guidelines to Facilitate Human-Wildlife Interactions in Conservation Translocations (2022) to inform planning and promote wildlife conservation, collaboration amongst groups and coexistence

Streptococcus intermedius causing infective endocarditis and abscesses: a report of three cases and review of the literature

<p>Abstract</p> <p>Background</p> <p><it>Streptococcus intermedius </it>is a member of the Streptococcus anginosus group. Clinical disease with <it>S. intermedius </it>is characterized by abscess formation and rarely endocarditis. Identification of <it>Streptococcus intermedius </it>is difficult, leading to the development of molecular methods to more accurately identify and characterize this organism.</p> <p>Case presentation</p> <p>Over a period of 6 months we encountered three cases of invasive <it>Streptococcus intermedius </it>infection presenting as hepatic abscesses, brain abscess, and endocarditis. We confirmed our microbiologic diagnosis through 16S sequencing and found a common virulence gene in each case.</p> <p>Conclusion</p> <p>Our report illustrates three different clinical manifestations due to <it>Streptococcus intermedius </it>infection that can be encountered in healthy individuals in a community hospital setting. To our knowledge, this is the first case of <it>Streptococcus intermedius </it>endocarditis confirmed by 16S sequencing analysis. The use of molecular methods may allow a better understanding of the epidemiology and pathogenesis of this organism.</p

The properties of Brightest Cluster Galaxies in the SDSS DR6 adaptive matched filter cluster catalogue

We study the properties of Brightest Cluster Galaxies (BCGs) drawn from a catalogue of more than 69000 clusters in the SDSS DR6 based on the adaptive matched filter technique (AMF, Szabo et al., 2010). Our sample consists of more than 14300 galaxies in the redshift range 0.1-0.3. We test the catalog by showing that it includes well-known BCGs which lie in the SDSS footprint. We characterize the BCGs in terms of r-band luminosities and optical colours as well as their trends with redshift. In particular, we define and study the fraction of blue BCGs, namely those that are likely to be missed by either colour-based cluster surveys and catalogues. Richer clusters tend to have brighter BCGs, however less dominant than in poorer systems. 4-9% of our BCGs are at least 0.3 mag bluer in the g-r colour than the red-sequence at their given redshift. Such a fraction decreases to 1-6% for clusters above a richness of 50, where 3% of the BCGs are 0.5 mag below the red-sequence. A preliminary morphological study suggests that the increase in the blue fraction at lower richnesses may have a non-negligible contribution from spiral galaxies. We show that a colour selection based on the g-r red-sequence or on a cut at colour u-r >2.2 can lead to missing the majority of such blue BCGs. We also extend the colour analysis to the UV range by cross-matching our catalogue with publicly available data from Galex GR4 and GR5. We show a clear correlation between offset from the optical red-sequence and the amount of UV-excess. Finally, we cross-matched our catalogue with the ACCEPT cluster sample (Cavagnolo et al., 2009), and find that blue BCGs tend to be in clusters with low entropy and short cooling times. That is, the blue light is presumably due to recent star formation associated to gas feeding by cooling flows. (abridged)Comment: 15 pages, 15 figures, submitted to MNRA

Star formation activities in early-type brightest cluster galaxies

We identify a total of 120 early-type Brightest Cluster Galaxies (BCGs) at 0.1<z<0.4 in two recent large cluster catalogues selected from the Sloan Digital Sky Survey (SDSS). They are selected with strong emission lines in their optical spectra, with both H{\alpha} and [O II]{\lambda}3727 line emission, which indicates significant ongoing star formation. They constitute about ~ 0.5% of the largest, optically-selected, low-redshift BCG sample, and the fraction is a strong function of cluster richness. Their star formation history can be well described by a recent minor and short starburst superimposed on an old stellar component, with the recent episode of star formation contributing on average only less than 1 percent of the total stellar mass. We show that the more massive star-forming BCGs in richer clusters tend to have higher star formation rate (SFR) and specific SFR (SFR per unit galaxy stellar mass). We also compare their statistical properties with a control sample selected from X-ray luminous clusters, and show that the fraction of star-forming BCGs in X-ray luminous clusters is almost one order of magnitude larger than that in optically-selected clusters. BCGs with star formation in cooling flow clusters usually have very flat optical spectra and show the most active star formation, which may be connected with cooling flows.Comment: 15 pages, 10 figures and 2 tables, accepted for publication in MNRA

The relation between stellar populations, structure and environment for dwarf elliptical galaxies from the MAGPOP-ITP

Dwarf galaxies, as the most numerous type of galaxy, offer the potential to study galaxy formation and evolution in detail in the nearby Universe. Although they seem to be simple systems at first view, they remain poorly understood. In an attempt to alleviate this situation, the MAGPOP EU Research and Training Network embarked on a study of dwarf galaxies named MAGPOP-ITP (Peletier et al., 2007). In this paper, we present the analysis of a sample of 24 dwarf elliptical galaxies (dEs) in the Virgo Cluster and in the field, using optical long-slit spectroscopy. We examine their stellar populations in combination with their light distribution and environment. We confirm and strengthen previous results that dEs are, on average, younger and more metal-poor than normal elliptical galaxies, and that their [alpha/Fe] abundance ratios scatter around solar. This is in accordance with the downsizing picture of galaxy formation where mass is the main driver for the star formation history. We also find new correlations between the luminosity-weighted mean age, the large-scale asymmetry, and the projected Virgocentric distance. We find that environment plays an important role in the termination of the star formation activity by ram pressure stripping of the gas in short timescales, and in the transformation of disky dwarfs to more spheroidal objects by harassment over longer timescales. This points towards a continuing infall scenario for the evolution of dEs.Comment: Accepted for publication in MNRAS: 22 pages, 13 figures and 9 table

Detection of exon skipping events in BRCA1 RNA using MLPA kit P002

A rapid and easy method to screen for aberrant cDNA would be a very useful diagnostic tool in genetics since a fraction of the DNA variants found affect RNA splicing. The currently used RT-PCR methods require new primer combinations to study each variant that might affect splicing. Since MLPA is routinely used to detect large genomic deletions and successfully detected exon skipping events in Duchenne muscular dystrophy in cDNA, we performed a pilot study to evaluate its value for BRCA1 cDNA. The effect of puromycin, DNase I and two different DNA cleaning protocols were tested in the RNA analysis of lymphocyte cultures. We used two samples from unrelated families with two different BRCA1 exon deletion events, two healthy unrelated controls and six samples from hereditary breast/ovarian cancer syndrome (HBOC) patients without BRCA1/2 mutations. Using RNA treated with DNase I and cleaned in a column system from puromycin-treated fractions, we were able to identify the two BRCA1 deletions. Additional HBOC patients did not show additional splice events. However, we were not able to get reproducible results. Therefore, the cDNA-MLPA technique using kit BRCA1 P002 is in our hands currently not reliable enough for routine RNA analysis and needs further optimization

Regulation of Orai1/STIM1 mediated ICRAC by intracellular pH

Ca²⁺ release activated Ca²⁺-(CRAC) channels composed of two cellular proteins, Ca²⁺-sensing stromal interaction molecule 1 (STIM1) and pore-forming Orai1, are the main mediators of the Ca²⁺ entry pathway activated in response to depletion of intracellular Ca²⁺ stores. Previously it has been shown that the amplitude of CRAC current (ICRAC) strongly depends on extracellular and intracellular pH. Here we investigate the intracellular pH (pHi) dependence of ICRAC mediated by Orai1 and STIM1ectopically expressed in HEK293 cells. The results indicate that pHi affects not only the amplitude of the current, but also Ca²⁺ dependent gating of CRAC channels. Intracellular acidification changes the kinetics of ICRAC, introducing prominent re-activation component in the currents recorded in response to voltage steps to strongly negative potentials. ICRAC with similar kinetics can be observed at normal pHi if the expression levels of Orai1 are increased, relative to the expression levels of STIM1. Mutations in the STIM1 inactivation domain significantly diminish the dependence of ICRAC kinetics on pHi, but have no effect on pHi dependence of ICRAC amplitude, implying that more than one mechanism is involved in CRAC channel regulation by intracellular pH.D. Gavriliouk, N.R. Scrimgeour, S. Grigoryev, L. Ma, F.H. Zhou, G.J. Barritt, G.Y. Rychko

Levels of Mycoplasma genitalium antimicrobial resistance differ by both region and gender in the state of Queensland, Australia: implications for treatment guidelines

is frequently associated with urogenital and rectal infections, with the number of cases of macrolide resistant and quinolone resistant continuing to increase. In this study, we examined the levels of antibiotic resistance to these two common antibiotic treatments in geographically distinct locations in Queensland, Australia. Samples were screened for macrolide resistance-associated mutations using a commercially available kit (ResistancePlus™ MG; SpeeDx) and quinolone resistance-associated mutations were identified by PCR and DNA sequencing. Comparisons between antibiotic resistance mutations and location/gender were performed. Levels of macrolide resistance were high across both locations (62%). Quinolone resistance mutations were found in ∼10% of all samples, with a number of samples harboring mutations conferring resistance to both macrolides and quinolones. Quinolone resistance was higher in Southeast Queensland, compared to North Queensland and this was consistent in both males and females ( = 0.007). Rectal samples from males harbored high levels of macrolide (75.9%) and quinolone (19%) resistance, with 15.5% harboring resistance to both classes of antibiotics. Overall the lowest observed level of resistance was for quinolones in females from North Queensland (1.6%). These data highlight the high levels of antibiotic resistance in within Queensland and the challenges faced by STI clinicians in managing these infections. The data do however show that levels of antibiotic resistance may differ between populations within the same state, which has implications for clinical management and treatment guidelines. These findings also support the need for ongoing antibiotic resistance surveillance and tailored treatment

Association of blood lipids with Alzheimer's disease: A comprehensive lipidomics analysis

Introduction: The aim of this study was to (1) replicate previous associations between six blood lipids and Alzheimer’s disease (AD) (Proitsi et al 2015) and (2) identify novel associations between lipids, clinical AD diagnosis, disease progression and brain atrophy (left/right hippocampus/entorhinal cortex). Methods: We performed untargeted lipidomic analysis on 148 AD and 152 elderly control plasma samples and used univariate and multivariate analysis methods. Results: We replicated our previous lipids associations and reported novel associations between lipids molecules and all phenotypes. A combination of 24 molecules classified AD patients with .70% accuracy in a test and a validation data set, and we identified lipid signatures that predicted disease progression (R2 5 0.10, test data set) and brain atrophy (R2 0.14, all test data sets except left entorhinal cortex). We putatively identified a number of metabolic features including cholesteryl esters/triglycerides and phosphatidylcholines. Discussion: Blood lipids are promising AD biomarkers that may lead to new treatment strategies