Characterizing the bacterial community associated with the model alcyoniid Lobophytum pauciflorum

Casey Whalen studied the bacterial community associated with microhabitats and developing larvae of the soft coral Lobophytum pauciflorum. He also identified in L. pauciflorum potential homologs of proteins with known antimicrobial properties in other Cnidaria. His findings contribute to our collective knowledge on host-symbiont interactions, especially in the understudied Octocorallia

Dispersion and Characterization of Nickel Nanostrands in Thermoset and Thermoplastic Polymers

Nickel Nanostrands (NiNS) are nano-particles that are highly branched and have a high aspect ratio. These particles show promise as excellent additives to composites when electrical conductivity is desired. Unfortunately, there is very little research done on dispersing powdered NiNS in various polymer matrices. This thesis covers the research in dispersing NiNS in three separate polymer systems, and related composite processing and characterization. An aromatic polyimide (CP2) is first used as a thermoplastic matrix and attempts to incorporate NiNS via an in-situ processing technique concurrent with in-situ polymerization are detailed. Epoxy is then used as a representative thermoset where the NiNS are dispersed in the resin before a hardener is added. The last polymer tested is thermoplastic Polyvinylidene Fluoride (PVDF). NiNS are introduced to this polymer in a solution mixture. Once dispersed, the PVDF solution is heated until the solvent evaporates leaving a PVDF melt containing NiNS, which is subsequently cooled. Samples of all three polymer nano-composites are created and dispersion is observed with an optical microscope. Using DSC, DMA and dielectric spectroscopy, thermal, mechanical and electrical properties are measured and analyzed. Results for the CP2 nano-composites showed that during the cure phase, the NiNS settled to the bottom of the films resulting in a non-dispersed composite. This result highlighted the difference between NiNS and other more conventional nano-particles, namely that the NiNS are larger and heavier, therefore are not 'locked into' a dispersed state by the polymer chains. Several techniques were investigated for dispersing NiNS in the epoxy matrix. A method without solvent was shown to be the most effective and resulted in a well-dispersed nano-composite that showed increases in electrical conductivity and dielectric constant as NiNS concentration increases. Enhancement in storage modulus was observed above the composite's Tg as well. PVDF nano-composites also showed good dispersion and a general increase in electrical properties. Below Tg, storage modulus decreases at first before a slight recovery with increasing NiNS. Beyond Tg, the opposite effect is observed. FTIR measurements for the PVDF were also taken and showed no significant changes in the polymer morphology with additions of NINS

Transcriptional networks specifying homeostatic and inflammatory programs of gene expression in human aortic endothelial cells.

Endothelial cells (ECs) are critical determinants of vascular homeostasis and inflammation, but transcriptional mechanisms specifying their identities and functional states remain poorly understood. Here, we report a genome-wide assessment of regulatory landscapes of primary human aortic endothelial cells (HAECs) under basal and activated conditions, enabling inference of transcription factor networks that direct homeostatic and pro-inflammatory programs. We demonstrate that 43% of detected enhancers are EC-specific and contain SNPs associated to cardiovascular disease and hypertension. We provide evidence that AP1, ETS, and GATA transcription factors play key roles in HAEC transcription by co-binding enhancers associated with EC-specific genes. We further demonstrate that exposure of HAECs to oxidized phospholipids or pro-inflammatory cytokines results in signal-specific alterations in enhancer landscapes and associate with coordinated binding of CEBPD, IRF1, and NFκB. Collectively, these findings identify cis-regulatory elements and corresponding trans-acting factors that contribute to EC identity and their specific responses to pro-inflammatory stimuli

Beluga Vocalizations Decrease in Response to Vessel Traffic in the Mackenzie River Estuary

Vessel traffic negatively affects marine mammals by causing behavioural disturbance, acoustic masking, contamination (i.e., oil spills), and ship strikes. Few studies have examined the effects of vessels on marine mammals in the Arctic, but beluga whales appear to be especially sensitive to vessel traffic. We examine how the vocalizations of belugas are impacted by vessel traffic in the Tarium Niryutait Marine Protected Area in the Mackenzie River estuary of the western Canadian Arctic. Between one and four acoustic recorders were deployed between June and August each year between 2015 and 2018 near the only shipping channel at this site. We examined beluga vocalizations from acoustic recordings over four summers and assessed how the distance to the nearest vessel passing the acoustic recorder affected the number of vocalizations. Beluga vocalizations within the range of the acoustic recorder decreased significantly when vessels were within 5 km of the acoustic recorder. This result suggests either that belugas are avoiding the vessel or that they reduce their vocalization in response to vessel traffic. Future work is needed to assess exactly how belugas are reacting to vessel traffic in this area and what the long-term consequences of these reactions are. Management measures for reducing these impacts must be carefully considered, especially since these vessels are very restricted in where they can travel, and many of the vessels are necessary for the livelihoods of local communities.La circulation maritime a des effets négatifs sur les mammifères marins, car elle entraîne des perturbations comportementales, masque leurs signaux acoustiques et engendre de la contamination (comme des déversements de pétrole) et des collisions. Bien que peu d’études aient examiné les effets des bateaux sur les mammifères marins de l’Arctique, les bélugas semblent particulièrement sensibles à la circulation maritime. Dans cet article, nous examinons en quoi les vocalisations des bélugas sont touchées par la circulation maritime dans la zone de protection marine de Tarium Niryutait faisant partie de l’estuaire du fleuve Mackenzie, dans l’ouest de l’Arctique canadien. Entre un et quatre enregistreurs acoustiques ont été déployés de juin à août de chaque année entre 2015 et 2018, à proximité du seul chenal de navigation de l’endroit. Nous avons examiné les vocalisations des bélugas prélevées à l’aide des enregistreurs acoustiques au cours de quatre étés, et évalué en quoi la distance du bateau passant le plus près de l’enregistreur acoustique avait un effet sur le nombre de vocalisations. Les vocalisations des bélugas dans la zone de l’enregistreur acoustique diminuaient considérablement lorsque les bateaux se trouvaient à moins de cinq kilomètres de l’enregistreur. Ce résultat suggère soit que les bélugas évitent les bateaux, soit qu’ils réduisent leurs vocalisations en réponse à la circulation maritime. Il y a lieu de pousser cette étude plus loin pour évaluer exactement comment les bélugas réagissent à la circulation des bateaux dans cette région, et quelles sont les conséquences à long terme de ces réactions. Il y a lieu aussi de considérer avec soin des mesures de gestion pour réduire ces incidences, surtout parce que les déplacements de ces bateaux sont assujettis à de nombreuses restrictions et parce que grand nombre des bateaux qui passent par là sont nécessaires à la subsistance des collectivités de la région. &nbsp

Short alleles, bigger smiles? The effect of 5-HTTLPR on positive emotional expressions.

The present research examined the effect of the 5-HTTLPR polymorphism in the serotonin transporter gene on objectively coded positive emotional expressions (i.e., laughing and smiling behavior objectively coded using the Facial Action Coding System). Three studies with independent samples of participants were conducted. Study 1 examined young adults watching still cartoons. Study 2 examined young, middle-aged, and older adults watching a thematically ambiguous yet subtly amusing film clip. Study 3 examined middle-aged and older spouses discussing an area of marital conflict (that typically produces both positive and negative emotion). Aggregating data across studies, results showed that the short allele of 5-HTTLPR predicted heightened positive emotional expressions. Results remained stable when controlling for age, gender, ethnicity, and depressive symptoms. These findings are consistent with the notion that the short allele of 5-HTTLPR functions as an emotion amplifier, which may confer heightened susceptibility to environmental conditions

Molecular techniques and their limitations shape our view of the holobiont

It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont

Whole Genome Resequencing Reveals Natural Target Site Preferences of Transposable Elements in Drosophila melanogaster

Transposable elements are mobile DNA sequences that integrate into host genomes using diverse mechanisms with varying degrees of target site specificity. While the target site preferences of some engineered transposable elements are well studied, the natural target preferences of most transposable elements are poorly characterized. Using population genomic resequencing data from 166 strains of Drosophila melanogaster, we identified over 8,000 new insertion sites not present in the reference genome sequence that we used to decode the natural target preferences of 22 families of transposable element in this species. We found that terminal inverted repeat transposon and long terminal repeat retrotransposon families present clade-specific target site duplications and target site sequence motifs. Additionally, we found that the sequence motifs at transposable element target sites are always palindromes that extend beyond the target site duplication. Our results demonstrate the utility of population genomics data for high-throughput inference of transposable element targeting preferences in the wild and establish general rules for terminal inverted repeat transposon and long terminal repeat retrotransposon target site selection in eukaryotic genomes

Composite Repair Performance at Elevated Temperatures

For the better part of the past 20 years composite materials have been used to repair damaged piping and pressurized components in plants, refineries, and pipelines. The use of composite materials has been accompanied by comprehensive research programs focused on the development and assessment of using composite technology for restoring integrity to damaged piping and pressurized components. Of particular interest are composite repair standards such as ISO 24817 and ASME PCC-2 that provide technical guidance in how to properly design composite repair systems."/jats:p" "jats:p"The vast body of research completed to date has involved assessments at ambient conditions; however, at the present time there is significant interest in evaluating the performance of composite repair materials at elevated temperatures. This paper is focused on the topic of high temperature composite repairs and addresses the critical role of utilizing temperature-based mechanical properties to establish a composite repair design. The backbone of this effort is the development of composite performance curves that correlate change in strength as a function of temperature. A discussion on supporting full-scale pressure test results are included, along with guidance for users in how to properly design composite repair systems for applications at elevated temperatures