Continuous Measurements of Water and Carbon Isotopes: Tools to Minimized Maritime and Coastal Vulnerabilities and Maximize Awareness (Integrating Primary-Secondary-Tertiary Systems)

United States Department of Homeland Security University of Alaska Anchorag

High aspect ratio template and method for producing same

Millimeter to nano-scale structures manufactured using a multi-component polymer fiber matrix are disclosed. The use of dissimilar polymers allows the selective dissolution of the polymers at various stages of the manufacturing process. In one application, biocompatible matrixes may be formed with long pore length and small pore size. The manufacturing process begins with a first polymer fiber arranged in a matrix formed by a second polymer fiber. End caps may be attached to provide structural support and the polymer fiber matrix selectively dissolved away leaving only the long polymer fibers. These may be exposed to another product, such as a biocompatible gel to form a biocompatible matrix. The polymer fibers may then be selectively dissolved leaving only a biocompatible gel scaffold with the pores formed by the dissolved polymer fibers

Growth rings show limited evidence for ungulates' potential to suppress shrubs across the Arctic

Global warming has pronounced effects on tundra vegetation, and rising mean temperatures increase plant growth potential across the Arctic biome. Herbivores may counteract the warming impacts by reducing plant growth, but the strength of this effect may depend on prevailing regional climatic conditions. To study how ungulates interact with temperature to influence growth of tundra shrubs across the Arctic tundra biome, we assembled dendroecological data from 20 sites, comprising 1153 individual shrubs and 223 63 annual growth rings. Evidence for ungulates suppressing shrub radial growth was only observed at intermediate summer temperatures (6.5 degrees C-9 degrees C), and even at these temperatures the effect was not strong. Multiple factors, including forage preferences and landscape use by the ungulates, and favourable climatic conditions enabling effective compensatory growth of shrubs, may weaken the effects of ungulates on shrubs, possibly explaining the weakness of observed ungulate effects. Earlier local studies have shown that ungulates may counteract the impacts of warming on tundra shrub growth, but we demonstrate that ungulates' potential to suppress shrub radial growth is not always evident, and may be limited to certain climatic conditions

Growth rings show limited evidence for ungulates’ potential to suppress shrubs across the Arctic

Global warming has pronounced effects on tundra vegetation, and rising mean temperatures increase plant growth potential across the Arctic biome. Herbivores may counteract the warming impacts by reducing plant growth, but the strength of this effect may depend on prevailing regional climatic conditions. To study how ungulates interact with temperature to influence growth of tundra shrubs across the Arctic tundra biome, we assembled dendroecological data from 20 sites, comprising 1153 individual shrubs and 223 63 annual growth rings. Evidence for ungulates suppressing shrub radial growth was only observed at intermediate summer temperatures (6.5 ◦C–9 ◦C), and even at these temperatures the effect was not strong. Multiple factors, including forage preferences and landscape use by the ungulates, and favourable climatic conditions enabling effective compensatory growth of shrubs, may weaken the effects of ungulates on shrubs, possibly explaining the weakness of observed ungulate effects. Earlier local studies have shown that ungulates may counteract the impacts of warming on tundra shrub growth, but we demonstrate that ungulates’ potential to suppress shrub radial growth is not always evident, and may be limited to certain climatic conditions.Research Council of Norwayhttp://iopscience.iop.org/1748-9326dm2022Mammal Research InstituteZoology and Entomolog

The tundra phenology database: More than two decades of tundra phenology responses to climate change

Observations of changes in phenology have provided some of the strongest signals of the effects of climate change on terrestrial ecosystems. The International Tundra Experiment (ITEX), initiated in the early 1990s, established a common protocol to measure plant phenology in tundra study areas across the globe. Today, this valuable collection of phenology measurements depicts the responses of plants at the colder extremes of our planet to experimental and ambient changes in temperature over the past decades. The database contains 150,434 phenology observations of 278 plant species taken at 28 study areas for periods of 1 to 26 years. Here we describe the full dataset to increase the visibility and use of these data in global analyses, and to invite phenology data contributions from underrepresented tundra locations. Portions of this tundra phenology database have been used in three recent syntheses, some datasets are expanded, others are from entirely new study areas, and the entirety of these data are now available at the Polar Data Catalogue (https://doi.org/10.21963/13215)

Leaf Tissue C:N is Modified by Growing Season and Goose Grazing Phenology in a Sub-Arctic Coastal Wetland of Western Alaska

There has been an advancement of spring conditions in coastal sub-arctic wetlands of the Yukon-Kuskokwim Delta. Pacific black brant (Branta bernicla nigricans) are dominant grazers in these coastal systems, and feed heavily on Carex subspathacea sedge lawns. Sedges have their highest nitrogen (N) concentration shortly following springtime emergence; however the greatest availability of N in the system occurs after hatch when nutrient demand for N by growing gosling and molting adults is greatest. We examine the influence of advanced growing season and different goose arrival times on vegetation biomass and C:N ratio. We set up fenced exclosures to control for grazing. Using a flock of captive wild brant, two plots received early, typical, late, or no grazing treatments to simulate different arrival times. To simulate an early growing season, we used fiberglass open-top chambers (OTCs) from May 1 to July 1. Half of our plots received the advanced growing treatment, while the remainder was exposed to ambient conditions. We found that grazing had a greater effect on plants than an advanced warming treatment. Early season grazing heavily reduced above- and belowground plant biomass. Plants responded to grazing by producing new leaves with higher N concentrations, lower C:N, and increased δ15N. An advanced growing season increased overall plant biomass, decreased C:N in plat tissue, and increased δ13C. While increased plant biomass might provide more forage, this might not be advantageous for geese if plants have lower N concentrations. Thus, the timing of the growing season and grazing both have important implications for C- and N-cycling and nutrient availability for geese in this system

Isotopic Discrimination of Aquifer Recharge Sources, Subsystem Connectivity and Flow Patterns in the South Fork Palouse River Basin, Idaho and Washington, USA

Groundwater studies in the South Fork Palouse River Basin have been unable to determine recharge sources, subsystem connectivity and flow patterns due to the discontinuity of pathways in the heterogeneous and anisotropic aquifers located in Columbia River flood basalts and interbedded sediments. Major ion, δ18O, δ2H, δ13C, δ34S and temperature for groundwater collected from 28 wells of varying depths indicate a primary recharge source dominated by snowmelt along the eastern basin margin. This recharge can be separated into two distinct sources—a deeper and relatively less altered snowmelt signal (−17.3‰ to −16.8‰ δ18O, −131‰ to −127‰ δ2H, −12.9‰ to −10‰ δ13C, 18⁻23 °C) and a more altered signal likely derived from a shallower mixture of snowmelt, precipitation and surface water (−16.1‰ to −15.5‰ δ18O, −121‰ to −117‰ δ2H, −15.9‰ to −12.9‰ δ13C, 12⁻19 °C). A mixing of the shallow and deep source waters is observed within the upper aquifer of the Grande Ronde Formation near Moscow, Idaho, which results in a homogenization of isotope ratios and geochemistry for groundwater at nearly any depth to the west of this mixing zone. This homogenized signal is prevalent in a likely primary productive zone of an intermediate depth in the overall aquifer system

Phenotypic and Genotypic Analysis of Amino Acid Auxotrophy in Lactobacillus helveticus CNRZ 32▿

The conversion of amino acids into volatile and nonvolatile compounds by lactic acid bacteria in cheese is thought to represent the rate-limiting step in the development of mature flavor and aroma. Because amino acid breakdown by microbes often entails the reversible action of enzymes involved in biosynthetic pathways, our group investigated the genetics of amino acid biosynthesis in Lactobacillus helveticus CNRZ 32, a commercial cheese flavor adjunct that reduces bitterness and intensifies flavor notes. Most lactic acid bacteria are auxotrophic for several amino acids, and L. helveticus CNRZ 32 requires 14 amino acids. The reconstruction of amino acid biosynthetic pathways from a draft-quality genome sequence for L. helveticus CNRZ 32 revealed that amino acid auxotrophy in this species was due primarily to gene absence rather than point mutations, insertions, or small deletions, with good agreement between gene content and phenotypic amino acid requirements. One exception involved the phenotypic requirement for Asp (or Asn), which genome predictions suggested could be alleviated by citrate catabolism. This prediction was confirmed by the growth of L. helveticus CNRZ 32 after the addition of citrate to a chemically defined medium that lacked Asp and Asn. Genome analysis also predicted that L. helveticus CNRZ 32 possessed ornithine decarboxylase activity and would therefore catalyze the conversion of ornithine to putrescine, a volatile biogenic amine. However, experiments to confirm ornithine decarboxylase activity in L. helveticus CNRZ 32 by the use of several methods were unsuccessful, which indicated that this bacterium likely does not contribute to putrescine production in cheese