The Effect of Inoculants on Nutrient Losses of Corn Silage and High-moisture Corn Stored in Mini Silos

Two experiments were conducted to determine the effects of inoculants (BONSILAGE CORN 200G and BONSILAGE HMC 200G) containing Lactobacillus buchneri, Lactobacillus plantarum and Pediococcus acidilactici on nutrient losses and aerobic stability of corn silage and high moisture corn. Corn silage and high moisture corn were inoculated and stored in mini silos with nutrient loss and spoilage characterizations at 30, 90, and 120 days with multiple inoculation levels. Longer ensiling times led to greater total acid production. The higher levels of inoculation led to lower total acid production and higher pH. Inoculating corn silage and high moisture corn also affected the fermentation process by decreasing lactic acid production and increasing acetic acid production. The increase in acetic acid production may be partially responsible for the increased aerobic stability observed for the inoculated feeds. Previous research would support our finding of greater stability and lower DM losses with L. buchneri inoculants

Effect of Three Initial Implant Programs with a Common Terminal Revalor®- 200 on Feedlot Performance and Carcass Traits of Weaned Steers

A commercial feedlot study utilizing 1,350 calf- fed steers (initial BW = 623 lb; ±23 lb) compared three initial implant strategies: Revalor®- IS (day 1), Revalor®- IS (day 1) and Revalor®- 200 (day 67), or Revalor®- XS (day 1). Each initial implant strategy was followed by a terminal Revalor®- 200 implant (day 133) to determine effects on performance and carcass traits. No differences in final body weight, intake, gain, or feed conversion were observed on either a live, or carcass adjusted basis. Th ere were also no differences in hot carcass weight, USDA quality grade, or USDA yield grade. Results from this study suggest initial implant strategy has minimal impact on feedlot and carcass performance when following with a terminal Revalor®- 200 implant

Effect of distillers grains moisture and inclusion level in livestock diets on greenhouse gas emissions in the corn-ethanol-livestock life cycle

A model was previously developed (Biofuel Energy Systems Simulator; www. bess.unl.edu) to predict greenhouse gas (GHG) emissions and net energy yield when ethanol is produced from corn. The model also predicts feedlot cattle, dairy cattle, and swine performance and feed replacement value of ethanol coproducts. Updated equations that predict performance of feedlot cattle fed 0 to 40% of dietary DM as corn wet (WDGS), modified (MDGS), or dry (DDGS) distillers grains plus solubles replacing dry-rolled and high-moisture corn were developed and incorporated into the model. Equations were derived from pen-level performance for 20 finishing studies evaluating WDGS, 4 evaluating MDGS, and 4 evaluating DDGS conducted at the University of Nebraska. Feeding value of WDGS was 145 to 131% of corn replaced when included at 20 to 40% of diet DM due to a quadratic (P \u3c 0.01) increase in G:F. The feeding value of MDGS was 124 to 117% with a quadratic (P \u3c 0.01) increase in G:F and 112 to 110% for DDGS with a linear (P \u3c 0.01) increase in G:F. Midwest corn-ethanol-livestock life cycle GHG reduction relative to gasoline (97.7 g CO2 equivalent/MJ of ethanol) was 61 to 57% when WDGS was fed to feedlot cattle for 20 to 40% diet inclusion. Feeding MDGS and DDGS to feedlot cattle reduced GHG emissions from the corn-ethanol-cattle system by 53 to 50% and 46 to 41%, respectively. Feeding WDGS to feedlot cattle was the optimum feed use of distillers grains plus solubles based on feeding performance and GHG reduction

Effect of Increasing Supplemental Rumen Undegradable Protein (RUP) on Performance of Calves Fed a Silage Growing Diet

A growing study evaluated the effects of increasing supplemental rumen undegradable protein (RUP) on performance of calves fed an 85% corn silage diet. Five levels of supplementation were evaluated with 12 individually fed steers per level of supplement. Supplement levels consisted of 0, 3.25, 6.5, 9.75 and 13% supplemental RUP (% of diet DM) fed as a blend of 60% Soypass and 40% Empyreal. Increasing supplemental RUP in the diet increased ending body weight and average daily gain linearly, and decreased F:G linearly while DMI remained constant among treatments. As cattle grew from 600 to 730 lb, F:G was improved 30% by supplying RUP. Th e same amount of RUP improved F:G by 11% as cattle grew beyond 730 lb. By meeting MP requirements, supplementing RUP linearly improved both ADG and F:G of growing calves, especially early in the growing period

Effect of Increasing Supplemental Rumen Undegradable Protein (RUP) on Performance of Calves Fed a Silage Growing Diet

A growing study evaluated the effects of increasing supplemental rumen undegradable protein (RUP) on performance of calves fed an 85% corn silage diet. Five levels of supplementation were evaluated with 12 individually fed steers per level of supplement. Supplement levels consisted of 0, 3.25, 6.5, 9.75 and 13% supplemental RUP (% of diet DM) fed as a blend of 60% Soypass and 40% Empyreal. Increasing supplemental RUP in the diet increased ending body weight and average daily gain linearly, and decreased F:G linearly while DMI remained constant among treatments. As cattle grew from 600 to 730 lb, F:G was improved 30% by supplying RUP. Th e same amount of RUP improved F:G by 11% as cattle grew beyond 730 lb. By meeting MP requirements, supplementing RUP linearly improved both ADG and F:G of growing calves, especially early in the growing period

FOXA1 mutations alter pioneering activity, differentiation and prostate cancer phenotypes.

Mutations in the transcription factor FOXA1 define a unique subset of prostate cancers but the functional consequences of these mutations and whether they confer gain or loss of function is unknown1-9. Here, by annotating the landscape of FOXA1 mutations from 3,086 human prostate cancers, we define two hotspots in the forkhead domain: Wing2 (around 50% of all mutations) and the highly conserved DNA-contact residue R219 (around 5% of all mutations). Wing2 mutations are detected in adenocarcinomas at all stages, whereas R219 mutations are enriched in metastatic tumours with neuroendocrine histology. Interrogation of the biological properties of wild-type FOXA1 and fourteen FOXA1 mutants reveals gain of function in mouse prostate organoid proliferation assays. Twelve of these mutants, as well as wild-type FOXA1, promoted an exaggerated pro-luminal differentiation program, whereas two different R219 mutants blocked luminal differentiation and activated a mesenchymal and neuroendocrine transcriptional program. Assay for transposase-accessible chromatin using sequencing (ATAC-seq) of wild-type FOXA1 and representative Wing2 and R219 mutants revealed marked, mutant-specific changes in open chromatin at thousands of genomic loci and exposed sites of FOXA1 binding and associated increases in gene expression. Of note, ATAC-seq peaks in cells expressing R219 mutants lacked the canonical core FOXA1-binding motifs (GTAAAC/T) but were enriched for a related, non-canonical motif (GTAAAG/A), which was preferentially activated by R219-mutant FOXA1 in reporter assays. Thus, FOXA1 mutations alter its pioneering function and perturb normal luminal epithelial differentiation programs, providing further support for the role of lineage plasticity in cancer progression

Statistical Interparticle Potential of an Ideal Gas of Non-Abelian Anyons

We determine and study the statistical interparticle potential of an ideal system of non-Abelian Chern-Simons (NACS) particles, comparing our results with the corresponding results of an ideal gas of Abelian anyons. In the Abelian case, the statistical potential depends on the statistical parameter and it has a "quasi-bosonic" behaviour for statistical parameter in the range (0,1/2) (non-monotonic with a minimum) and a "quasi-fermionic" behaviour for statistical parameter in the range (1/2,1) (monotonically decreasing without a minimum). In the non-Abelian case the behavior of the statistical potential depends on the Chern- Simons coupling and the isospin quantum number: as a function of these two parameters, a phase diagram with quasi-bosonic, quasi-fermionic and bosonic-like regions is obtained and investigated. Finally, using the obtained expression for the statistical potential, we compute the second virial coefficient of the NACS gas, which correctly reproduces the results available in literature.Comment: 21 pages, 4 color figure

Comparison of Commercial Lick Tubs to Distillers Grains Supplementation for Calves Grazing Corn Residue

Steer calves grazing irrigated corn residue were supplemented dried distillers grains plus solubles (DGS) or allowedcontinuous access to a commercial lick tub. Dried DGS was fed at 2.94 lb/steer/day and the lick tubs were consumed at 2.04 lb/steer/day (DM basis). Gain was greater for cattle supplemented with dried DGS (1.36 lb/day) compared to those with access to lick tubs (0.83 lb/day). Supplement efficiency varied between calves receiving dried DGS (46%) and those with continuous access to the lick tub (43%) when expressed on a DM basis. Values for dried DGS supplementation (48%) were not different for supplement efficiencyon an OM basis when compared to cattle on the lick tub treatment (50%). Economic analysis shows that as the price of DGS increases, the difference in profit between supplementation strategiesis reduced

FIGS -- Faint Infrared Grism Survey: Description and Data Reduction

The Faint Infrared Grism Survey (FIGS) is a deep Hubble Space Telescope (HST) WFC3/IR (Wide Field Camera 3 Infrared) slitless spectroscopic survey of four deep fields. Two fields are located in the Great Observatories Origins Deep Survey-North (GOODS-N) area and two fields are located in the Great Observatories Origins Deep Survey-South (GOODS-S) area. One of the southern fields selected is the Hubble Ultra Deep Field. Each of these four fields were observed using the WFC3/G102 grism (0.8$\mu m$-1.15$\mu m$ continuous coverage) with a total exposure time of 40 orbits (~ 100 kilo-seconds) per field. This reaches a 3 sigma continuum depth of ~26 AB magnitudes and probes emission lines to $\approx 10^{-17}\ erg\ s^{-1} \ cm^{-2}$. This paper details the four FIGS fields and the overall observational strategy of the project. A detailed description of the Simulation Based Extraction (SBE) method used to extract and combine over 10000 spectra of over 2000 distinct sources brighter than m_F105W=26.5 mag is provided. High fidelity simulations of the observations is shown to significantly improve the background subtraction process, the spectral contamination estimates, and the final flux calibration. This allows for the combination of multiple spectra to produce a final high quality, deep, 1D-spectra for each object in the survey.Comment: 21 Pages. 17 Figures. To appear in Ap

Use of weekly, low dose, high frequency ultrasound for hard to heal venous leg ulcers: the VenUS III randomised controlled trial

Objective To assess the clinical effectiveness of weekly delivery of low dose, high frequency therapeutic ultrasound in conjunction with standard care for hard to heal venous leg ulcers