Evidence for an Intermediate-mass Milky Way from Gaia DR2 Halo Globular Cluster Motions

We estimate the mass of the Milky Way (MW) within 21.1 kpc using the kinematics of halo globular clusters (GCs) determined by Gaia. The second Gaia data release (DR2) contained a catalogue of absolute proper motions (PMs) for a set of Galactic GCs and satellite galaxies measured using Gaia DR2 data. We select from the catalogue only halo GCs, identifying a total of 34 GCs spanning $2.0 < r < 21.1$ kpc, and use their 3D kinematics to estimate the anisotropy over this range to be $\beta = 0.46^{+0.15}_{-0.19}$, in good agreement, though slightly lower than, a recent estimate for a sample of halo GCs using HST PM measurements further out in the halo. We then use the Gaia kinematics to estimate the mass of the MW inside the outermost GC to be $M(< 21.1 \mathrm{kpc}) = 0.21^{+0.04}_{-0.03} 10^{12} \mathrm{M_\odot}$, which corresponds to a circular velocity of $v_\mathrm{circ}(21.1 \mathrm{kpc}) = 206^{+19}_{-16}$ km/s. The implied virial mass is $M_\mathrm{virial} = 1.28^{+0.97}_{-0.48} 10^{12} \mathrm{M_\odot}$. The error bars encompass the uncertainties on the anisotropy and on the density profile of the MW dark halo, and the scatter inherent in the mass estimator we use. We get improved estimates when we combine the Gaia and HST samples to provide kinematics for 46 GCs out to 39.5 kpc: $\beta = 0.52^{+0.11}_{-0.14}$, $M(< 39.5 \mathrm{kpc}) = 0.42^{+0.07}_{-0.06} 10^{12} \mathrm{M_\odot}$, and $M_\mathrm{virial} = 1.54^{+0.75}_{-0.44} 10^{12} \mathrm{M_\odot}$. We show that these results are robust to potential substructure in the halo GC distribution. While a wide range of MW virial masses have been advocated in the literature, from below $10^{12} \mathrm{M_\odot}$ to above $2 \times 10^{12}\mathrm{M_\odot}$, these new data imply that an intermediate mass is most likely

The peculiar kinematics of the multiple populations in the globular cluster Messier 80 (NGC 6093)

We combine MUSE spectroscopy and Hubble Space Telescope ultraviolet (UV) photometry to perform a study of the chemistry and dynamics of the Galactic globular cluster Messier 80 (M80, NGC 6093). Previous studies have revealed three stellar populations that not only vary in their light-element abundances, but also in their radial distributions, with concentration decreasing with increasing nitrogen enrichment. This remarkable trend, which sets M80 apart from the other Galactic globular clusters, points towards a complex formation and evolutionary history. To better understand how M80 formed and evolved, revealing its internal kinematics is key. We find that the most N-enriched population rotates faster than the other two populations at a 2 sigma confidence level. While our data further suggest that the intermediate population shows the least amount of rotation, this trend is rather marginal (1 - 2 sigma). Using axisymmetric Jeans models, we show that these findings can be explained from the radial distributions of the populations if they possess different angular momenta. Our findings suggest that the populations formed with primordial kinematical differences

Breaking beta: A comparison of mass modelling methods for spherical systems

We apply four different mass modelling methods to a suite of publicly available mock data for spherical stellar systems. We focus on the recovery of the density and velocity anisotropy as a function of radius, using either line-of-sight velocity data only, or adding proper motion data. All methods perform well on isotropic and tangentially anisotropic mock data, recovering the density and velocity anisotropy within their 95% confidence intervals over the radial range 0.25 < R/Rhalf < 4, where Rhalf is the half light radius. However, radially-anisotropic mocks are more challenging. For line-of-sight data alone, only methods that use information about the shape of the velocity distribution function are able to break the degeneracy between the density profile and the velocity anisotropy to obtain an unbiased estimate of both. This shape information can be obtained through directly fitting a global phase space distribution function, by using higher order 'Virial Shape Parameters', or by assuming a Gaussian velocity distribution function locally, but projecting it self-consistently along the line of sight. Including proper motion data yields further improvements, and in this case, all methods give a good recovery of both the radial density and velocity anisotropy profiles

Effectiveness of guided self-help in decreasing expressed emotion in family caregivers of people diagnosed with depression in Thailand: a randomised controlled trial

Background: High expressed emotion (EE) can extend the duration of illness and precipitate relapse; however, little evidence-based information is available to assist family caregivers of individuals with depression. In the present exploratory study, we examined the effectiveness of a cognitive behaviour therapy (CBT) based guided self-help (GSH) manual in decreasing EE in caregivers of people with depression, in Thailand. Method: A parallel group randomised controlled trial was conducted, following CONSORT guidelines, with 54 caregivers who were allocated equally to GSH or control group (standard outpatient department support). In addition, both groups were contacted weekly by telephone. EE was assessed, using the Family Questionnaire (FQ), at baseline, post-test (Week 8) and follow-up (Week 12). Results: FQ scores at baseline indicated that both groups had similar, though moderately high level of EE. However, between baseline and post-test EE scores decreased markedly in the intervention group, but in contrast, they increased slightly in the control group. Between post-test and follow-up, little change took place in the EE scores of either group. Overall, the intervention group recipients of GSH showed a significant decrease in EE whereas the control group recipients of standard outpatient department support reported a slight increase in EE. Conclusion: These findings provide preliminary evidence that GSH is beneficial in reducing EE in caregivers, which is advantageous to family members with depression and caregivers. The approach may be used as an adjunct to the limited outpatient department support given to caregivers by mental health professionals and, perhaps, to caregivers who do not attend these departments

Effects of sample handling and storage on quantitative lipid analysis in human serum

There is sparse information about specific storage and handling protocols that minimize analytical error and variability in samples evaluated by targeted metabolomics. Variance components that affect quantitative lipid analysis in a set of human serum samples were determined. The effects of freeze-thaw, extraction state, storage temperature, and freeze-thaw prior to density-based lipoprotein fractionation were quantified. The quantification of high abundance metabolites, representing the biologically relevant lipid species in humans, was highly repeatable (with coefficients of variation as low as 0.01 and 0.02) and largely unaffected by 1–3 freeze-thaw cycles (with 0–8% of metabolites affected in each lipid class). Extraction state had effects on total lipid class amounts, including decreased diacylglycerol and increased phosphatidylethanolamine in thawed compared with frozen samples. The effects of storage temperature over 1 week were minimal, with 0–4% of metabolites affected by storage at 4°C, −20°C, or −80°C in most lipid classes, and 19% of metabolites in diacylglycerol affected by storage at −20°C. Freezing prior to lipoprotein fractionation by density ultracentrifugation decreased HDL free cholesterol by 37% and VLDL free fatty acid by 36%, and increased LDL cholesterol ester by 35% compared with fresh samples. These findings suggest that density-based fractionation should preferably be undertaken in fresh serum samples because up to 37% variability in HDL and LDL cholesterol could result from a single freeze-thaw cycle. Conversely, quantitative lipid analysis within unfractionated serum is minimally affected even with repeated freeze-thaw cycles

Giant serous cystadenoma arising from an accessory ovary in a morbidly obese 11-year-old girl: a case report

<p>Abstract</p> <p>Introduction</p> <p>Ectopic ovarian tissue is an unusual entity, especially if it is an isolated finding thought to be of embryological origin.</p> <p>Case presentation</p> <p>An 11-year-old, morbidly obese female presented with left flank pain, nausea, and irregular menses. Various diagnostic procedures suggested a large ovarian cyst, and surgical resection was performed.</p> <p>Conclusion</p> <p>Histologically, the resected mass was not of tubal origin as suspected, but a serous cystadenoma arising from ovarian tissue. The patient's two normal, eutopic ovaries were completely uninvolved and unaffected. A tumor arising from ectopic ovarian tissue of embryological origin seems the most likely explanation. We suggest refining the descriptive nomenclature so as to more precisely characterize the various presentations of ovarian ectopia.</p

Mutation of the diamond-blackfan anemia gene Rps7 in mouse results in morphological and neuroanatomical phenotypes.

The ribosome is an evolutionarily conserved organelle essential for cellular function. Ribosome construction requires assembly of approximately 80 different ribosomal proteins (RPs) and four different species of rRNA. As RPs co-assemble into one multi-subunit complex, mutation of the genes that encode RPs might be expected to give rise to phenocopies, in which the same phenotype is associated with loss-of-function of each individual gene. However, a more complex picture is emerging in which, in addition to a group of shared phenotypes, diverse RP gene-specific phenotypes are observed. Here we report the first two mouse mutations (Rps7(Mtu) and Rps7(Zma)) of ribosomal protein S7 (Rps7), a gene that has been implicated in Diamond-Blackfan anemia. Rps7 disruption results in decreased body size, abnormal skeletal morphology, mid-ventral white spotting, and eye malformations. These phenotypes are reported in other murine RP mutants and, as demonstrated for some other RP mutations, are ameliorated by Trp53 deficiency. Interestingly, Rps7 mutants have additional overt malformations of the developing central nervous system and deficits in working memory, phenotypes that are not reported in murine or human RP gene mutants. Conversely, Rps7 mouse mutants show no anemia or hyperpigmentation, phenotypes associated with mutation of human RPS7 and other murine RPs, respectively. We provide two novel RP mouse models and expand the repertoire of potential phenotypes that should be examined in RP mutants to further explore the concept of RP gene-specific phenotypes

Lonely but avoidant—the unfortunate juxtaposition of loneliness and self-disgust

Loneliness is prevalent worldwide and is a known risk factor for numerous physical and mental health outcomes. The health consequences of chronic loneliness coupled with the cost on public health care has necessitated the development of interventions and campaigns to end loneliness globally. According to a recent meta-analysis, such interventions focus on improving social skills, increasing opportunities for social contact/support (i.e., reducing social isolation) or addressing maladaptive cognition (e.g., irrational thoughts, self-defeating, and self-blame thoughts). The results showed that changing maladaptive thoughts offer “the best chance” for alleviating feelings of loneliness. In accordance with the latter approach, this paper proposes a new paradigm in understanding and treating loneliness that takes into account self-disgust, an aversive self-conscious affective state that reflects disgust directed towards the self. Based on findings from published and unpublished data, it is argued that interventions against loneliness that focus exclusively on improving social skills and increasing opportunities for social contact may be ineffective because lonelier people experience more self-disgust, which makes them more socially inhibited and reluctant to connect with other people. Future interventions should consider self-disgust in the treatment of loneliness and explore ways to counter feelings of self-disgust

Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure

Extracellular heat-shock protein 72 (eHsp72) concentration increases during exercise-heat stress when conditions elicit physiological strain. Differences in severity of environmental and exercise stimuli have elicited varied response to stress. The present study aimed to quantify the extent of increased eHsp72 with increased exogenous heat stress, and determine related endogenous markers of strain in an exercise-heat model. Ten males cycled for 90 min at 50% O2peak in three conditions (TEMP, 20°C/63% RH; HOT, 30.2°C/51%RH; VHOT, 40.0°C/37%RH). Plasma was analysed for eHsp72 pre, immediately post and 24-h post each trial utilising a commercially available ELISA. Increased eHsp72 concentration was observed post VHOT trial (+172.4%) (P<0.05), but not TEMP (-1.9%) or HOT (+25.7%) conditions. eHsp72 returned to baseline values within 24hrs in all conditions. Changes were observed in rectal temperature (Trec), rate of Trec increase, area under the curve for Trec of 38.5°C and 39.0°C, duration Trec ≥ 38.5°C and ≥ 39.0°C, and change in muscle temperature, between VHOT, and TEMP and HOT, but not between TEMP and HOT. Each condition also elicited significantly increasing physiological strain, described by sweat rate, heart rate, physiological strain index, rating of perceived exertion and thermal sensation. Stepwise multiple regression reported rate of Trec increase and change in Trec to be predictors of increased eHsp72 concentration. Data suggests eHsp72 concentration increases once systemic temperature and sympathetic activity exceeds a minimum endogenous criteria elicited during VHOT conditions and is likely to be modulated by large, rapid changes in core temperature

Validation of the Comprehensive Feeding Practices Questionnaire with parents of 10-to-12-year-olds

Abstract Background: There is a lack of validated instruments for quantifying feeding behavior among parents of older children and adolescents. The Comprehensive Feeding Practices Questionnaire (CFPQ) is a self-report measure to assess multiple parental feeding practices. The CFPQ is originally designed for use with parents of children ranging in age from about 2 to 8 years. It is previously validated with American and French parents of children within this age range. The aim of the present study was to adapt and test the validity of this measure with parents of older children (10-to-12-year-olds) in a Norwegian setting. Methods: A sample of 963 parents of 10-to-12-year-olds completed a Norwegian, slightly adapted version of the CFPQ. Scale analyses were performed to test the validity of the instrument in our sample. Results: Although a few problematic items and scales were revealed, scale analyses showed that the psychometric properties of the slightly adapted, Norwegian version of the CFPQ were surprisingly similar to those of the original CFPQ. Conclusions: Our results indicated that the CFPQ, with some small modifications, is a valid tool for measuring multiple parental feeding practices with parents of 10-to12-year-olds