Formulation of linguistic regression model based on natural words

When human experts express their ideas and thoughts, human words are basically employed in these expressions. That is, the experts with much professional experiences are capable of making assessment using their intuition and experiences. The measurements and interpretation of characteristics are taken with uncertainty, because most measured characteristics, analytical result, and field data can be interpreted only intuitively by experts. In such cases, judgments may be expressed using linguistic terms by experts. The difficulty in the direct measurement of certain characteristics makes the estimation of these characteristics imprecise. Such measurements may be dealt with the use of fuzzy set theory. As Professor L. A. Zadeh has placed the stress on the importance of the computation with words, fuzzy sets can take a central role in handling words [12, 13]. In this perspective fuzzy logic approach is offten thought as the main and only useful tool to deal with human words. In this paper we intend to present another approach to handle human words instead of fuzzy reasoning. That is, fuzzy regression analysis enables us treat the computation with words. In order to process linguistic variables, we define the vocabulary translation and vocabulary matching which convert linguistic expressions into membership functions on the interval [0–1] on the basis of a linguistic dictionary, and vice versa. We employ fuzzy regression analysis in order to deal with the assessment process of experts from linguistic variables of features and characteristics of an objective into the linguistic expression of the total assessment. The presented process consists of four portions: (1) vocabulary translation, (2) estimation, (3) vocabulary matching and (4) dictionary. We employed fuzzy quantification theory type 2 for estimating the total assessment in terms of linguistic structural attributes which are obtained from an expert

Genetic Changes to a Transcriptional Silencer Element Confers Phenotypic Diversity within and between Drosophila Species

The modification of transcriptional regulation has become increasingly appreciated as a major contributor to morphological evolution. However, the role of negative-acting control elements (e.g. silencers) in generating morphological diversity has been generally overlooked relative to positive-acting “enhancer” elements. The highly variable body coloration patterns among Drosophilid insects represents a powerful model system in which the molecular alterations that underlie phenotypic diversity can be defined. In a survey of pigment phenotypes among geographically disparate Japanese populations of Drosophila auraria, we discovered a remarkable degree of variation in male-specific abdominal coloration. In testing the expression patterns of the major pigment-producing enzymes, we found that phenotypes uniquely correlated with differences in the expression of ebony, a gene required for yellow-colored cuticle. Assays of ebony’s transcriptional control region indicated that a lightly pigmented strain harbored cis-regulatory mutations that caused correlated changes in its expression. Through a series of chimeric reporter constructs between light and dark strain alleles, we localized function-altering mutations to a conserved silencer that mediates a male-specific pattern of ebony repression. This suggests that the light allele was derived through the loss of this silencer’s activity. Furthermore, examination of the ebony gene of D. serrata, a close relative of D. auraria which secondarily lost male-specific pigmentation revealed the parallel loss of this silencer element. These results demonstrate how loss-of-function mutations in a silencer element resulted in increased gene expression. We propose that the mutational inactivation of silencer elements may represent a favored path to evolve gene expression, impacting morphological traits

Drosophila suzukii: the genetic footprint of a recent, world-wide invasion

Native to Asia, the soft-skinned fruit pest Drosophila suzukii has recently invaded the United States and Europe. The eastern United States represents the most recent expansion of their range, and presents an opportunity to test alternative models of colonization history. Here we investigate the genetic population structure of this invasive fruit fly, with a focus on the eastern United States. We sequenced six X-linked gene fragments from 246 individuals collected from a total of 12 populations. We examine patterns of genetic diversity within and between populations and explore alternative colonization scenarios using Approximate Bayesian Computation. Our results indicate high levels of nucleotide diversity in this species and suggest that the recent invasions of Europe and the continental United States are independent demographic events. More broadly speaking, our results highlight the importance of integrating population structure into demographic models, particularly when attempting to reconstruct invasion histories. Finally, our simulation results illustrate the general challenge of reconstructing invasion histories using genetic data and suggest that genome-level data are often required to distinguish among alternative demographic scenarios

Effects of empagliflozin in patients with chronic kidney disease from Japan: exploratory analyses from EMPA–KIDNEY

Background: EMPA–KIDNEY assessed the effects of empagliflozin 10 mg once daily vs. placebo in 6609 patients with chronic kidney disease (CKD) at risk of progression, including 612 participants from Japan. Methods: Eligibility required an estimated glomerular filtration rate (eGFR) of ≥ 20  Results: Japanese participants had higher levels of albuminuria and eGFR than those from non-Japan regions. During a median of 2.0 year follow-up, a primary outcome occurred in 432 patients (13.1%) in the empagliflozin group and in 558 patients (16.9%) in the placebo group (hazard ratio [HR], 0.72, 95% confidence interval [95%CI] 0.64–0.82; P  Conclusions: Empagliflozin safely reduced the risk of “kidney disease progression or cardiovascular death” in patients with CKD, with consistent effects in participants from Japan

Binomial Numeral Systems: Description and Aplications to Numeration Problems

We develop a new class of positional numeral systems, namely the binomial ones, which form a subclass of generalized positional numeral systems (GPNS). The binomial systems have wide range of applications in the information transmission, processing, and storage due to their error-detection capabilities. In this paper, the binomial numeral systems are well-defined, their prefix and compactness properties are established. Algorithms of generating binomial coding words (non-uniform and uniform) are presented, as well as an enhanced procedure of construction of constant weight Boolean combinations based upon the non-uniform binomial coding words. The correctness of this procedure is also established

Effect of the re-use of the osmotic solution on the stability of osmodehydro-refrigerated grapefruit

Osmotic dehydration (OD) of grapefruit (55 degrees Brix sucrose solution, 30 degrees C) was carried out to obtain 75 g water/100 g sample in the final product. Although the grapefruit was replaced each time, the osmotic solution (OS) was reused for five OD cycles, with or without pasteurization. The samples obtained in cycles 1, 3 and 5, were stored at 10 degrees C. Changes in degrees Brix, water content, water activity, pH, total acidity, ascorbic acid content, cation concentration, respiration rate and total microbial counts at different storage times were analyzed and compared to fresh-cut grapefruit stored under the same conditions. During OD, a partial loss of the natural soluble substances present in the fruit was observed. In terms of the dehydration level reached by the fruit, it is possible to reuse the OS in up to 5 OD, without any reconcentration treatment. Nevertheless, it is advisable to pasteurize the OS before each cycle in order to obtain a product with a shelf-life of between 7 and 12 days in refrigeration, depending on the number of cyclesThe authors thank the Ministerio de Ciencia y Tecnologia and the Fondo Europeo de Desarrollo Regional (FEDER) for the financial support throughout the project AGL2005-05994.Moraga Ballesteros, MJ.; Moraga Ballesteros, G.; Martínez Navarrete, N. (2011). Effect of the re-use of the osmotic solution on the stability of osmodehydro-refrigerated grapefruit. Food Science and Technology. 44(1):35-41. https://doi.org/10.1016/j.lwt.2010.05.018S354144

EWS/FLI Mediates Transcriptional Repression via NKX2.2 during Oncogenic Transformation in Ewing's Sarcoma

EWS/FLI is a master regulator of Ewing's sarcoma formation. Gene expression studies in A673 Ewing's sarcoma cells have demonstrated that EWS/FLI downregulates more genes than it upregulates, suggesting that EWS/FLI, and/or its targets, function as transcriptional repressors. One critical EWS/FLI target, NKX2.2, is a transcription factor that contains both transcriptional activation and transcriptional repression domains, raising the possibility that it mediates portions of the EWS/FLI transcriptional signature. We now report that microarray analysis demonstrated that the transcriptional profile of NKX2.2 consists solely of downregulated genes, and overlaps with the EWS/FLI downregulated signature, suggesting that NKX2.2 mediates oncogenic transformation via transcriptional repression. Structure-function analysis revealed that the DNA binding and repressor domains in NKX2.2 are required for oncogenesis in Ewing's sarcoma cells, while the transcriptional activation domain is completely dispensable. Furthermore, blockade of TLE or HDAC function, two protein families thought to mediate the repressive function of NKX2.2, inhibited the transformed phenotype and reversed the NKX2.2 transcriptional profile in Ewing's sarcoma cells. Whole genome localization studies (ChIP-chip) revealed that a significant portion of the NKX2.2-repressed gene expression signature was directly mediated by NKX2.2 binding. These data demonstrate that the transcriptional repressive function of NKX2.2 is necessary, and sufficient, for the oncogenic phenotype of Ewing's sarcoma, and suggest a therapeutic approach to this disease

Pdx1 Is Post-Translationally Modified In vivo and Serine 61 Is the Principal Site of Phosphorylation

Maintaining sufficient levels of Pdx1 activity is a prerequisite for proper regulation of blood glucose homeostasis and beta cell function. Mice that are haploinsufficient for Pdx1 display impaired glucose tolerance and lack the ability to increase beta cell mass in response to decreased insulin signaling. Several studies have shown that post-translational modifications are regulating Pdx1 activity through intracellular localization and binding to co-factors. Understanding the signaling cues converging on Pdx1 and modulating its activity is therefore an attractive approach in diabetes treatment. We employed a novel technique called Nanofluidic Proteomic Immunoassay to characterize the post-translational profile of Pdx1. Following isoelectric focusing in nano-capillaries, this technology relies on a pan specific antibody for detection and it therefore allows the relative abundance of differently charged protein species to be examined simultaneously. In all eukaryotic cells tested we find that the Pdx1 protein separates into four distinct peaks whereas Pdx1 protein from bacteria only produces one peak. Of the four peaks in eukaryotic cells we correlate one of them to a phosphorylation Using alanine scanning and mass spectrometry we map this phosphorylation to serine 61 in both Min6 cells and in exogenous Pdx1 over-expressed in HEK293 cells. A single phosphorylation is also present in cultured islets but it remains unaffected by changes in glucose levels. It is present during embryogenesis but is not required for pancreas development