Geographies of landscape aesthetics : mapping landscape terminology in digitised historical travel accounts of Loch Lomond and the Trossachs

Acknowledgements Ogg.: Conceptualisation, Methodology, Formal Analysis, Investigation, Visualisation, Writing –Original Draft Preparation. Wartmann.: Conceptualisation, Supervision, Writing – Review & Editing.Peer reviewedPublisher PD

Exploring tranquillity experienced in landscapes based on social media

This work was financially supported by the Swiss National Science Foundation [grant no. 171911] and the European Union's Horizon 2020 research and innovation programme [grant agreement no. 689,812] (‘LandSense’).Peer reviewedPublisher PD

Relating landscape ecological metrics with public survey data on perceived landscape quality and place attachment

Context It is essential for policy-making and planning that we understand landscapes not only in terms of landscape ecological patterns, but also in terms of their contribution to people's quality of life. Objectives In this study our objective is to test relationships between landscape ecology and social science indicators, by investigating how landscape patterns are linked to people’s perception of landscape quality. Methods To assess public views on landscapes we conducted a survey among 858 respondents in Switzerland. We combined this survey data on perceived landscape quality and place attachment with landscape metrics (e.g. diversity, naturalness of land cover, urban sprawl, fragmentation) in a statistical model to test hypotheses about the relationships between the different variables of interest. Results Our results illustrate the contribution of both landscape composition metrics and social science indicators to understanding variation in people’s perception and assessment of landscape. For example, we found the landscape ecology metrics on urban sprawl and fragmentation to be a negative predictor of overall satisfaction with landscape, and that perceived landscape quality positively predicted place attachment and satisfaction with the municipality landscape. Conclusions This study highlights the importance and feasibility of combining landscape ecology metrics and public survey data on how people perceive, value and relate to landscape in an integrated manner. Our approach has the potential for implementation across a variety of settings and can contribute to holistic and integrated landscape assessments that combine ecological and socio-cultural aspects

Levels of the Autophagy-Related 5 Protein Affect Progression and Metastasis of Pancreatic Tumors in Mice

[Background and Aims]: Cells in pancreatic ductal adenocarcinoma (PDAC) undergo autophagy, but its effects vary with tumor stage and genetic factors. We investigated the consequences of varying levels of the autophagy related 5 (Atg5) protein on pancreatic tumor formation and progression. [Methods]: We generated mice that express oncogenic Kras in primary pancreatic cancer cells and have homozygous disruption of Atg5 (A5;Kras) or heterozygous disruption of Atg5 (A5+/–;Kras), and compared them with mice with only oncogenic Kras (controls). Pancreata were analyzed by histology and immunohistochemistry. Primary tumor cells were isolated and used to perform transcriptome, metabolome, intracellular calcium, extracellular cathepsin activity, and cell migration and invasion analyses. The cells were injected into wild-type littermates, and orthotopic tumor growth and metastasis were monitored. Atg5 was knocked down in pancreatic cancer cell lines using small hairpin RNAs; cell migration and invasion were measured, and cells were injected into wild-type littermates. PDAC samples were obtained from independent cohorts of patients and protein levels were measured on immunoblot and immunohistochemistry; we tested the correlation of protein levels with metastasis and patient survival times. [Results]: A5+/–;Kras mice, with reduced Atg5 levels, developed more tumors and metastases, than control mice, whereas A5;Kras mice did not develop any tumors. Cultured A5+/–;Kras primary tumor cells were resistant to induction and inhibition of autophagy, had altered mitochondrial morphology, compromised mitochondrial function, changes in intracellular Ca2+ oscillations, and increased activity of extracellular cathepsin L and D. The tumors that formed in A5+/–;Kras mice contained greater numbers of type 2 macrophages than control mice, and primary A5+/–;Kras tumor cells had up-regulated expression of cytokines that regulate macrophage chemoattraction and differentiation into M2 macrophage. Knockdown of Atg5 in pancreatic cancer cell lines increased their migratory and invasive capabilities, and formation of metastases following injection into mice. In human PDAC samples, lower levels of ATG5 associated with tumor metastasis and shorter survival time. [Conclusions]: In mice that express oncogenic Kras in pancreatic cells, heterozygous disruption of Atg5 and reduced protein levels promotes tumor development, whereas homozygous disruption of Atg5 blocks tumorigenesis. Therapeutic strategies to alter autophagy in PDAC should consider the effects of ATG5 levels to avoid the expansion of resistant and highly aggressive cells.This study was supported in part by the Mildred-Scheel-Professur der Deutschen Krebshilfe 111464, DFG AL 1174/6-1 to H.A., DFG DI 2299/1-1 to K.N.D., DFG SFB1321 (S01) to K.S. and W.W., and the German Federal Ministry of Education and Research to the German Center for Diabetes Research (DZD e.V.) to J.A

Low loss coatings for the VIRGO large mirrors

présentée par L. PinardThe goal of the VIRGO program is to build a giant Michelson type interferometer (3 kilometer long arms) to detect gravitational waves. Large optical components (350 mm in diameter), having extremely low loss at 1064 nm, are needed. Today, the Ion beam Sputtering is the only deposition technique able to produce optical components with such performances. Consequently, a large ion beam sputtering deposition system was built to coat large optics up to 700 mm in diameter. The performances of this coater are described in term of layer uniformity on large scale and optical losses (absorption and scattering characterization). The VIRGO interferometer needs six main mirrors. The first set was ready in June 2002 and its installation is in progress on the VIRGO site (Italy). The optical performances of this first set are discussed. The requirements at 1064 nm are all satisfied. Indeed, the absorption level is close to 1 ppm (part per million), the scattering is lower than 5 ppm and the R.M.S. wavefront of these optics is lower than 8 nm on 150 mm in diameter. Finally, some solutions are proposed to further improve these performances, especially the absorption level (lower than 0.1 ppm) and the mechanical quality factor Q of the mirrors (thermal noise reduction)

Site Fidelity in Space Use by Spider Monkeys (Ateles geoffroyi) in the Yucatan Peninsula, Mexico

Animal home ranges may vary little in their size and location in the short term but nevertheless show more variability in the long term. We evaluated the degree of site fidelity of two groups of spider monkeys (Ateles geoffroyi) over a 10- and 13-year period, respectively, in the northeastern Yucatan peninsula, Mexico. We used the Local Convex Hull method to estimate yearly home ranges and core areas (defined as the 60% probability contour) for the two groups. Home ranges varied from 7.7 to 49.6 ha and core areas varied from 3.1 to 9.2 ha. We evaluated the degree of site fidelity by quantifying the number of years in which different areas were used as either home ranges or core areas. Large tracts were used only as home ranges and only for a few years, whereas small areas were used as either core area or home range for the duration of the study. The sum of the yearly core areas coincided partially with the yearly home ranges, indicating that home ranges contain areas used intermittently. Home ranges, and especially core areas, contained a higher proportion of mature forest than the larger study site as a whole. Across years and only in one group, the size of core areas was positively correlated with the proportion of adult males in the group, while the size of home ranges was positively correlated with both the proportion of males and the number of tree species included in the diet. Our findings suggest that spider monkey home ranges are the result of a combination of long-term site fidelity and year-to-year use variation to enable exploration of new resources

Negative feedback regulation of the ERK1/2 MAPK pathway

The extracellular signal-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signalling pathway regulates many cellular functions, including proliferation, differentiation, and transformation. To reliably convert external stimuli into specific cellular responses and to adapt to environmental circumstances, the pathway must be integrated into the overall signalling activity of the cell. Multiple mechanisms have evolved to perform this role. In this review, we will focus on negative feedback mechanisms and examine how they shape ERK1/2 MAPK signalling. We will first discuss the extensive number of negative feedback loops targeting the different components of the ERK1/2 MAPK cascade, specifically the direct posttranslational modification of pathway components by downstream protein kinases and the induction of de novo gene synthesis of specific pathway inhibitors. We will then evaluate how negative feedback modulates the spatiotemporal signalling dynamics of the ERK1/2 pathway regarding signalling amplitude and duration as well as subcellular localisation. Aberrant ERK1/2 activation results in deregulated proliferation and malignant transformation in model systems and is commonly observed in human tumours. Inhibition of the ERK1/2 pathway thus represents an attractive target for the treatment of malignant tumours with increased ERK1/2 activity. We will, therefore, discuss the effect of ERK1/2 MAPK feedback regulation on cancer treatment and how it contributes to reduced clinical efficacy of therapeutic agents and the development of drug resistance

Present and future evolution of advanced breast cancer therapy

Although the introduction of novel therapies and drug combinations has improved the prognosis of metastatic breast cancer, the disease remains incurable. Increased knowledge of the biology and the molecular alterations in breast cancer has facilitated the design of targeted therapies. These agents include receptor and nonreceptor tyrosine kinase inhibitors (epidermal growth factor receptor family), intracellular signaling pathways (phosphatidylinositol-3-kinase, AKT, mammalian target of rapamycin) angiogenesis inhibitors and agents that interfere with DNA repair (poly(ADP-ribose) polymerase inhibitors). In the present review, we present the most promising studies of these new targeted therapies and novel combinations of targeted therapies with cytotoxic agents

Assessing the potential of social media for estimating recreational use of urban and peri-urban forests

Social forest functions including recreation are important for increasingly urbanised societies. For effective management of forest recreation areas, monitoring visitor frequencies is crucial. Increasingly, attempts are being made to incorporate recreational use data into National Forest Inventories (NFI), but given the large scale of national assessments, such data is often elusive. In this study we explore the potential of geotagged social media data for assessing visitor frequencies and explore recreational activities through text-based social media data. We analysed data from Twitter, Flickr and Instagram, both at local scale for 10 NFI forest sites, as well as at national scale to assess recreational use. Data availability was significantly correlated between the three platforms, even though absolute counts differed markedly. The model of recreational visitation based on social media data correlated significantly with an existing potential recreational model, indicating that social media data are a valid source of information for recreational use and can be used in future studies to assess recreational potential. Although data availability limits assessments for small areas of forests, large scale assessments using social media are feasible, and provide a potentially more empirically grounded assessment of recreational potential than theoretical models alone. We suggest that future work should aim at integrating social media data into traditional theoretical recreational models as part of a method triangulation, particularly for areas where recreational usage by visitors is high, but population counts are low. However, because social media data are provided by commercial platforms, we believe that more research is needed into harvesting and analysing other forms of content generated by users to decrease the dependency on commercial social media platforms that may or may not be available in the long run, and can be run locally or through central organisations involved in forest and landscape monitoring and observation

StarBorn: Towards making in‐situ land cover data generation fun with a location‐based game

Data contributed by a large number of non‐experts is increasingly used to validate and curate land cover data, with location‐based games (LBGs) developed for this purpose generating particular interest. We here present our findings on StarBorn, a novel LBG with a strong focus on game play. Users conquer game‐tiles by visiting real‐world locations and collecting land cover data. Within three months, StarBorn generated 13,319 land cover classifications by 84 users. Results show that data are concentrated around users’ daily life spaces, agreement among users is highest for urban and industry land cover, and user‐generated land cover classifications exhibit high agreement with an authoritative data set. However, we also observe low user retention rates and negative correlations between number of contributions and agreement rates with an authoritative land cover product. We recommend that future work consider not only game play, but also how motivational aspects influence behavior and data quality. We conclude that LBGs are suitable tools for generating cost‐efficient in‐situ land cover classifications