Compaction, aeration and addition of mycotoxin contaminated silage alters the fermentation profile, mycotoxin content and aerobic stability of ryegrass (Lolium perenne) silage

This study investigated the effect of compaction, aeration and addition of a naturally mycotoxin contaminated ryegrass silage (MCS) containing 1803 µg/kg DM penicillic acid, on the nutritional value and mycotoxin content after ensiling and subsequent aerobic stability of ryegrass Lolium perenne silage (second-cut, June 2020, UK). Mini silos (30 L) were filled with differential compaction (500 kg FW/m3 and 333 kg FW/m3), aeration by injection of air (1L per 48h for 30d then 1L per 7d) and addition of MCS (1.5 g/kg FW ensiled forage) in a 2×2×2 factorial design. During ensilage, mean CO2% (kg FW) in the aerated silos increased with low compaction. Crude protein (CP) increased and ash decreased with aeration. Mean silage fermentation end products acetic (AA), lactic (LA) and propionic acid (PA) concentrations increased with MCS. PA concentration increased with aeration/low compaction. LA decreased and ethanol increased with low compaction. Mycotoxin profiles differed between the silages on opening and after 14-days incubation in aerobic conditions with disappearance of fusarenon X and penicillic acid and appearance of mycophenolic acid and roquefortine C (318 µg/kg DM and 890 µg/kg DM). Addition of MCS, increased the concentration of penicillic acid on opening with an interaction with aeration (80.6µg/kg DM MCS × aerated, 40.0 µg/kg DM in the MCS × sealed). Aerobic stability was affected by aeration and low compaction with reduced time taken to heat to +5°C and +10°C above ambient temperature, higher rate of increase in pH and higher cumulative temperatures to the first peak temperature. Higher mycotoxin concentration at opening had a similar effect increasing time to heat +5°C and +10°C above ambient temperatures in aerobic conditions. Regression analysis showed predominantly direct relationships between silage fermentation end-product concentrations and aerobic stability. This study revealed interactions between silage bacteria and fungi activity from the concentrations of fermentation end-products and mycotoxins during ensilage and subsequent aerobic spoilage. The results supported current best practice for silage making, promoting conditions for improved preservation and aerobic stability. The addition of MCS had unexpected positive effects. However, factors associated with the MCS benefiting aerobic stability were not determined

Whole-genome sequencing provides new insights into the clonal architecture of Barrett's esophagus and esophageal adenocarcinoma.

The molecular genetic relationship between esophageal adenocarcinoma (EAC) and its precursor lesion, Barrett's esophagus, is poorly understood. Using whole-genome sequencing on 23 paired Barrett's esophagus and EAC samples, together with one in-depth Barrett's esophagus case study sampled over time and space, we have provided the following new insights: (i) Barrett's esophagus is polyclonal and highly mutated even in the absence of dysplasia; (ii) when cancer develops, copy number increases and heterogeneity persists such that the spectrum of mutations often shows surprisingly little overlap between EAC and adjacent Barrett's esophagus; and (iii) despite differences in specific coding mutations, the mutational context suggests a common causative insult underlying these two conditions. From a clinical perspective, the histopathological assessment of dysplasia appears to be a poor reflection of the molecular disarray within the Barrett's epithelium, and a molecular Cytosponge technique overcomes sampling bias and has the capacity to reflect the entire clonal architecture

Bayesian multinomial probit modeling of daily windows of susceptibility for maternal PM2.5 exposure and congenital heart defects

Epidemiologic studies suggest that maternal ambient air pollution exposure during critical periods of pregnancy is associated with adverse effects on fetal development. In this work, we introduce new methodology for identifying critical periods of development during post-conception gestational weeks 2–8 where elevated exposure to particulate matter less than 2.5 µm (PM2.5) adversely impacts development of the heart. Past studies have focused on highly aggregated temporal levels of exposure during the pregnancy and have failed to account for anatomical similarities between the considered congenital heart defects. We introduce a multinomial probit model in the Bayesian setting that allows for joint identification of susceptible daily periods during pregnancy for 12 types of congenital heart defects with respect to maternal PM2.5 exposure. We apply the model to a dataset of mothers from the National Birth Defect Prevention Study where daily PM2.5 exposures from post-conception gestational weeks 2–8 are assigned using predictions from the downscaler pollution model. This approach is compared with two aggregated exposure models that define exposure as the average value over post-conception gestational weeks 2–8 and the average over individual weeks, respectively. Results suggest an association between increased PM2.5 exposure on post-conception gestational day 53 with the development of pulmonary valve stenosis and exposures during days 50 and 51 with tetralogy of Fallot. Significant associations are masked when using the aggregated exposure models. Simulation study results suggest that the findings are robust to multiple sources of error. The general form of the model allows for different exposures and health outcomes to be considered in future applications

The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control.

The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha

Isotropic-nematic phase equilibria in the Onsager theory of hard rods with length polydispersity

We analyse the effect of a continuous spread of particle lengths on the phase behavior of rodlike particles, using the Onsager theory of hard rods. Our aim is to establish whether ``unusual'' effects such as isotropic-nematic-nematic (I-N-N) phase separation can occur even for length distributions with a single peak. We focus on the onset of I-N coexistence. For a log-normal distribution we find that a finite upper cutoff on rod lengths is required to make this problem well-posed. The cloud curve, which tracks the density at the onset of I-N coexistence as a function of the width of the length distribution, exhibits a kink; this demonstrates that the phase diagram must contain a three-phase I-N-N region. Theoretical analysis shows that in the limit of large cutoff the cloud point density actually converges to zero, so that phase separation results at any nonzero density; this conclusion applies to all length distributions with fatter-than-exponentail tails. Finally we consider the case of a Schulz distribution, with its exponential tail. Surprisingly, even here the long rods (and hence the cutoff) can dominate the phase behaviour, and a kink in the cloud curve and I-N-N coexistence again result. Theory establishes that there is a nonzero threshold for the width of the length distribution above which these long rod effects occur, and shows that the cloud and shadow curves approach nonzero limits for large cutoff, both in good agreement with the numerical results.Comment: 20 pages, 13 figure

Investigating magmatic processes in the early Solar System using the Cl isotopic systematics of eucrites

Generally, terrestrial rocks, martian and chondritic meteorites exhibit a relatively narrow range in bulk and apatite Cl isotope compositions, with δ37Cl (per mil deviation from standard mean ocean chloride) values between − 5.6 and + 3.8 ‰. Lunar rocks, however, have more variable bulk and apatite δ37Cl values, ranging from ∼ − 4 to + 40 ‰. As the Howardite-Eucrite-Diogenite (HED) meteorites represent the largest suite of crustal and sub-crustal rocks available from a differentiated basaltic asteroid (4 Vesta), studying them for their volatiles may provide insights into planetary differentiation processes during the earliest Solar System history. Here the abundance and isotopic composition of Cl in apatite were determined for seven eucrites representing a broad range of textural and petrological characteristics. Apatite Cl abundances range from ∼ 25 to 4900 ppm and the δ37Cl values range from − 3.98 to + 39.2 ‰. Samples with lower apatite H2O contents were typically also enriched in 37Cl but no systematic correlation between δ37Cl and δD values was observed across samples. Modelled Rayleigh fractionation and a strong positive correlation between bulk δ66Zn and apatite δ37Cl support the hypothesis that Cl degassed as metal chlorides from eucritic magmas, in a hydrogen-poor environment. In the case of lunar samples, it has been noted that δ37Cl values of apatite positively correlate with bulk La/Yb ratio. Interestingly, most eucrites show a negative correlation with bulk La/Yb ratio. Recently, isotopically light Cl values have been suggested to record the primary solar nebular signature. If this is the case then 4 Vesta, which accreted rapidly and early in Solar System history, could also record this primary nebular signature corresponding to the lightest Cl values measured here. The significant variation in Cl isotope composition observed within the eucrites are likely related to degassing of metal chlorides

Iron and bismuth bound human serum transferrin reveals a partially-opened conformation in the N-lobe

Human serum transferrin (hTF) binds Fe(III) tightly but reversibly, and delivers it to cells via a receptor-mediated endocytosis process. The metal-binding and release result in significant conformational changes of the protein. Here, we report the crystal structures of diferric-hTF (Fe N Fe C-hTF) and bismuth-bound hTF (Bi N Fe C-hTF) at 2.8 and 2.4 Å resolutions respectively. Notably, the N-lobes of both structures exhibit unique 'partially-opened' conformations between those of the apo-hTF and holo-hTF. Fe(III) and Bi(III) in the N-lobe coordinate to, besides anions, only two (Tyr95 and Tyr188) and one (Tyr188) tyrosine residues, respectively, in contrast to four residues in the holo-hTF. The C-lobe of both structures are fully closed with iron coordinating to four residues and a carbonate. The structures of hTF observed here represent key conformers captured in the dynamic nature of the transferrin family proteins and provide a structural basis for understanding the mechanism of metal uptake and release in transferrin families. © 2012 Macmillan Publishers Limited. All rights reserved.published_or_final_versio

Technical summary

Human interference with the climate system is occurring. Climate change poses risks for human and natural systems. The assessment of impacts, adaptation, and vulnerability in the Working Group II contribution to the IPCC's Fifth Assessment Report (WGII AR5) evaluates how patterns of risks and potential benefits are shifting due to climate change and how risks can be reduced through mitigation and adaptation. It recognizes that risks of climate change will vary across regions and populations, through space and time, dependent on myriad factors including the extent of mitigation and adaptation

Propagating Disturbances in Coronal Loops: A Detailed Analysis of Propagation Speeds

Quasi-periodic disturbances have been observed in the outer solar atmosphere for many years now. Although first interpreted as upflows (Schrijver et al. (1999)), they have been widely regarded as slow magnetoacoustic waves, due to observed velocities and periods. However, recent observations have questioned this interpretation, as periodic disturbances in Doppler velocity, line width and profile asymmetry were found to be in phase with the intensity oscillations (De Pontieu et al. (2010),Tian1 et al. (2011))}, suggesting the disturbances could be quasi-periodic upflows. Here we conduct a detailed analysis of the velocities of these disturbances across several wavelengths using the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamics Observatory (SDO). We analysed 41 examples, including both sunspot and non sunspot regions of the Sun. We found that the velocities of propagating disturbances (PDs) located at sunspots are more likely to be temperature dependent, whereas the velocities of PDs at non sunspot locations do not show a clear temperature dependence. We also considered on what scale the underlying driver is affecting the properties of the PDs. Finally, we found that removing the contribution due to the cooler ions in the 193 A wavelength suggests that a substantial part of the 193 emission of sunspot PDs can be contributed to the cool component of 193\AA.Comment: 26 Papges, 15 Figure