195 research outputs found
Evidence for microbial mediation of subseafloor nitrogen redox processes at Loihi Seamount, Hawaii
© The Author(s), 2016. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Geochimica et Cosmochimica Acta 198 (2017): 131-150, doi:10.1016/j.gca.2016.10.029.The role of nitrogen cycling in submarine hydrothermal systems is far less studied than
that of other biologically reactive elements such as sulfur and iron. In order to address
this knowledge gap, we investigated nitrogen redox processes at Loihi Seamount,
Hawaii, using a combination of biogeochemical and isotopic measurements,
bioenergetic calculations and analysis of the prokaryotic community composition in
venting fluids sampled during four cruises in 2006, 2008, 2009 and 2013.
Concentrations of NH4+ were positively correlated to dissolved Si and negatively
correlated to NO3-+NO2-, while NO2- was not correlated to NO3-+NO2-, dissolved Si or
NH4+. This is indicative of hydrothermal input of NH4+ and biological mediation
influencing NO2- concentrations. The stable isotope ratios of NO3- (d15N and d18O) was
elevated with respect to background seawater, with d18O values exhibiting larger
changes than corresponding d15N values, reflecting the occurrence of both production
and reduction of NO3- by an active microbial community. d15N-NH4+ values ranged from
0‰ to +16.7‰, suggesting fractionation during consumption and potentially N-fixation
as well. Bioenergetic calculations reveal that several catabolic strategies involving the
reduction of NO3- and NO2- coupled to sulfide and iron oxidation could provide energy to
microbes in Loihi fluids, while 16S rRNA gene sequencing of Archaea and Bacteria in
the fluids reveals groups known to participate in denitrification and N-fixation. Taken
together, our data support the hypothesis that microbes are mediating N-based redox
processes in venting hydrothermal fluids at Loihi Seamount.This work
was supported by the NSF Microbial Observatories program (MCB 0653265), the
Gordon and Betty Moore Foundation (GBMF1609), NSF-OCE 0648287, the Center for
Dark Energy Biosphere Investigations (C-DEBI) and the NASA Astrobiology Institute — Life Underground (NAI-LU). Sequence data was generated as part of the Alfred P.
Sloan Foundation's ICoMM field project and the W. M. Keck Foundation
Effects of three low-volume, high-intensity exercise conditions on affective valence
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. A common barrier to exercise is “lack of time”. Accordingly, interest in low-volume, high-intensity training has grown exponentially since this activity is considered time-efficient. However, the high-intensity nature of this exercise may frequently result in feelings of displeasure creating another barrier for many people. The purpose of this study was to compare affective (pleasure-displeasure) responses to three low-volume, high-intensity exercise conditions, including a novel shortened-sprint protocol. Using a within-subjects, randomised crossover experiment, healthy participants (N = 36) undertook a single bout of: 1) traditional reduced-exertion, high-intensity interval training (TREHIT), 2) a novel, shortened-sprint REHIT (SSREHIT) protocol, and 3) sprint continuous training (SCT). Affect and perceived efforts were recorded throughout exercise using the Feeling Scale (FS) and the 15-point Borg Rating of Perceived Exertion (RPE) scale, respectively. Enjoyment was recorded 5 min post-exercise using the Exercise Enjoyment Scale (EES). Differences were found for FS (condition by time interaction: P = 0.01GG, η2 = 0.26), RPE (P = 0.01GG, η2 = 0.23), and enjoyment (P < 0.01) with all outcomes favouring SSREHIT. Shortened-sprint protocols may diminish feelings of displeasure and might be a time-efficient yet tolerable exercise choice to help motivate some people to increase their physical activity and fitness
The rise and fall of methanotrophy following a deepwater oil-well blowout
The blowout of the Macondo oil well in the Gulf of Mexico in April 2010 injected up to 500,000 tonnes of natural gas, mainly methane, into the deep sea1. Most of the methane released was thought to have been consumed by marine microbes between July and August 20102, 3. Here, we report spatially extensive measurements of methane concentrations and oxidation rates in the nine months following the spill. We show that although gas-rich deepwater plumes were a short-lived feature, water column concentrations of methane remained above background levels throughout the rest of the year. Rates of microbial methane oxidation peaked in the deepwater plumes in May and early June, coincident with a rapid rise in the abundance of known and new methane-oxidizing microbes. At this time, rates of methane oxidation reached up to 5,900 nmol l−1 d−1—the highest rates documented in the global pelagic ocean before the blowout4. Rates of methane oxidation fell to less than 50 nmol l−1 d−1 in late June, and continued to decline throughout the remainder of the year. We suggest the precipitous drop in methane consumption in late June, despite the persistence of methane in the water column, underscores the important role that physiological and environmental factors play in constraining the activity of methane-oxidizing bacteria in the Gulf of Mexico
Happiness through leisure
Happiness is important to individuals. If one were to make a judgment based on the vast
amount of self-help books available in any bookstore, the conclusion would have to be that
happiness is a very important aspect of people’s lives. Whether such books actually provide
any solutions to increase happiness is doubtful (Bergsma, 2008). Nevertheless, many are
clearly interested in happiness
Culture and achievement motivation in sport: A qualitative comparative study between Maghrebian and European French adolescents
Mineralogical and geochemical analysis of Fe-phases in drill-cores from the Triassic Stuttgart Formation at Ketzin CO₂ storage site before CO₂ arrival
Reactive iron (Fe) oxides and sheet silicate-bound Fe in reservoir rocks may affect the subsurface storage of CO2 through several processes by changing the capacity to buffer the acidification by CO2 and the permeability of the reservoir rock: (1) the reduction of three-valent Fe in anoxic environments can lead to an increase in pH, (2) under sulphidic conditions, Fe may drive sulphur cycling and lead to the formation of pyrite, and (3) the leaching of Fe from sheet silicates may affect silicate diagenesis. In order to evaluate the importance of Fe-reduction on the CO2 reservoir, we analysed the Fe geochemistry in drill-cores from the Triassic Stuttgart Formation (Schilfsandstein) recovered from the monitoring well at the CO2 test injection site near Ketzin, Germany. The reservoir rock is a porous, poorly to moderately cohesive fluvial sandstone containing up to 2–4 wt% reactive Fe. Based on a sequential extraction, most Fe falls into the dithionite-extractable Fe-fraction and Fe bound to sheet silicates, whereby some Fe in the dithionite-extractable Fe-fraction may have been leached from illite and smectite. Illite and smectite were detected in core samples by X-ray diffraction and confirmed as the main Fe-containing mineral phases by X-ray absorption spectroscopy. Chlorite is also present, but likely does not contribute much to the high amount of Fe in the silicate-bound fraction. The organic carbon content of the reservoir rock is extremely low (<0.3 wt%), thus likely limiting microbial Fe-reduction or sulphate reduction despite relatively high concentrations of reactive Fe-mineral phases in the reservoir rock and sulphate in the reservoir fluid. Both processes could, however, be fuelled by organic matter that is mobilized by the flow of supercritical CO2 or introduced with the drilling fluid. Over long time periods, a potential way of liberating additional reactive Fe could occur through weathering of silicates due to acidification by CO2
Community shift of ammonia-oxidizing bacteria along an anthropogenic pollution gradient from the Pearl River Delta to the South China Sea
The phylogenetic diversity and abundance of ammonia-oxidizing beta-proteobacteria (beta-AOB) was analyzed along an anthropogenic pollution gradient from the coastal Pearl River Delta to the South China Sea using the ammonia monooxygenase subunit A (amoA) gene. Along the gradient from coastal to the open ocean, the phylogenetic diversity of the dominant genus changed from Nitrosomonas to Nitrosospira, indicating the niche specificity by these two genera as both salinity and anthropogenic influence were major factors involved. The diversity of bacterial amoA gene was also variable along the gradient, with the highest in the deep-sea sediments, followed by the marshes sediments and the lowest in the coastal areas. Within the Nitrosomonas-related clade, four distinct lineages were identified including a putative new one (A5-16) from the different sites over the large geographical area. In the Nitrosospira-related clade, the habitat-specific lineages to the deep-sea and coastal sediments were identified. This study also provides strong support that Nitrosomonas genus, especially Nitrosomonas oligotropha lineage (6a) could be a potential bio-indicator species for pollution or freshwater/wastewater input into coastal environments. A suite of statistical analyses used showed that water depth and temperature were major factors shaping the community structure of beta-AOB in this study area
Enrichment and characterization of ammonia-oxidizing archaea from the open ocean : phylogeny, physiology and stable isotope fractionation
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in The ISME Journal 5 (2011): 1796–1808, doi:10.1038/ismej.2011.58.Archaeal genes for ammonia oxidation are widespread in the marine environment, but
direct physiological evidence for ammonia oxidation by marine archaea is limited. We
report the enrichment and characterization of three strains of pelagic ammonia-oxidizing
archaea (AOA) from the north Pacific Ocean that have been maintained in laboratory
culture for over three years. Phylogenetic analyses indicate the three strains belong to a
previously identified clade of water column-associated AOA and possess 16S rRNA
genes and ammonia monooxygenase subunit a (amoA) genes highly similar (98-99%
identity) to those recovered in DNA and cDNA clone libraries from the open ocean. The
strains grow in natural seawater-based liquid medium while stoichiometrically converting
ammonium (NH4
+) to nitrite (NO2
-). Ammonia oxidation by the enrichments is only
partially inhibited by allylthiourea at concentrations known to inhibit cultivated
ammonia-oxidizing bacteria. The three strains were used to determine the nitrogen stable
isotope effect (15εNH3) during archaeal ammonia oxidation, an important parameter for
interpreting stable isotope ratios in the environment. Archaeal 15εNH3 ranged from 13-
41‰, within the range of that previously reported for ammonia-oxidizing bacteria.
Despite low amino acid identity between the archaeal and bacterial Amo proteins, their
functional diversity as captured by 15εNH3 is similar.This work was supported by a Woods Hole Oceanographic Institution (WHOI)
Postdoctoral Scholar fellowship to AES and the WHOI Ocean Life Institute
Seasonal variations in the nitrogen isotopic composition of settling particles at station K2 in the western subarctic North Pacific
Intensive observations using hydrographical cruises and moored sediment trap deployments during 2010 and 2012 at station K2 in the North Pacific western subarctic gyre (WSG) revealed seasonal changes in δ15N of both suspended and settling particles. Suspended particles (SUS) were collected from depths between the surface and 200 m; settling particles by drifting traps (DST; 100-200 m) and moored traps (MST; 200 and 500 m). All particles showed higher δ15N values in winter and lower in summer, contrary to the expected by isotopic fractionation during phytoplankton nitrate consumption. We suggest that these observed isotopic patterns are due to ammonium consumption via light-controlled nitrification, which could induce variations in δ15N(SUS) of 0.4-3.1 ‰ in the euphotic zone (EZ). The δ15N(SUS) signature was reflected by δ15 N(DST) despite modifications during biogenic transformation from suspended particles in the EZ. δ15 N enrichment (average: 3.6 ‰) and the increase in C:N ratio (by 1.6) in settling particles suggests year-round contributions of metabolites from herbivorous zooplankton as well as TEPs produced by diatoms. Accordingly, seasonal δ15 N(DST) variations of 2.4-7.0 ‰ showed a significant correlation with primary productivity (PP) at K2. By applying the observed δ15 N(DST) vs. PP regression to δ15 N(MST) of 1.9-8.0 ‰, we constructed the first annual time-series of PP changes in the WSG. Moreover, the monthly export ratio at 500 m was calculated using both estimated PP and measured organic carbon fluxes. Results suggest a 1.6 to 1.8 times more efficient transport of photosynthetically-fixed carbon to the intermediate layers occurs in summer/autumn rather than winter/spring
Autonomous Application of Quantitative PCR in the Deep Sea: In Situ Surveys of Aerobic Methanotrophs Using the Deep-Sea Environmental Sample Processor
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