1,180 research outputs found

    Detection of Two Anaerobic Periodontopathogens in Children by Means of the BANA and ELISA Assays

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    The mouths of young children become colonized by a variety of bacteria, but there have been only a few studies that have sought the presence of periodontopathic species in this population. Almost all of these studies used culturing techniques rather than the newer detection methodologies for various periodontopathogens. Studies in adults have shown that Treponema denticola and Porphyromonas (Bacteroides) gingivalis can be detected in dental plaque by use of the BANA and ELISA diagnostic tests. In the present study, plaque samples from four subgingival sites in each of 157 children (aged from two to 18 years) were tested for BANA hydrolysis with a BANA reagent card, and for T. denticola and P. gingivalis with an ELISA assay. Anaerobic periodontopathogens hydrolyzing the BANA substrate were found to be present in at least one of four plaque samples in 88 children (56%). T. denticola and/or P. gingivalis were detected by ELISA in at least one plaque sample in each of 135 children (86%). This study shows that children are widely colonized by these micro-organisms. A higher proportion of Black children than Caucasian children was colonized by these BANA-positive organisms. Also, children having a parent with a documented history of periodontal disease were more likely to be BANA-positive than were children of parents with unknown periodontal status.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67961/2/10.1177_00220345910700070701.pd

    Quantifying the effect of particle characteristics on wheel/rail adhesion & damage through high pressure torsion testing

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    Low adhesion in the wheel/rail contact is a problem for the rail industry in Great Britain as it causes significant scheduling and safety issues. Applying sand to the wheel/rail contact is used to mitigate against low adhesion however, there is not a consensus on what makes a “good” particle for restoring adhesion, especially with regards to when the particle has entered the wheel/rail contact. The aim of this work was to investigate what particle characteristics had the greatest effect on wheel/rail adhesion and surface conditions, using a process of particle characterisation, tribological testing and statistical modelling. Particle size, shape, and hardness were all found to affect tribological performance. This knowledge can help guide future changes to sanding operations

    Prominent bulk pinning effect in the MgB_2 superconductor

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    We report the magnetic-field dependence of the irreversible magnetization of the recently discovered binary superconductor MgB2_{2}. For the temperature region of T<0.9TcT< 0.9T_c, the contribution of the bulk pinning to the magnetization overwhelms that of the surface pinning. This was evident from the fact that the magnetization curves, M(H)M(H), were well described by the critical-state model without considering the surface pinning effect. It was also found that the M(H)M(H) curves at various temperatures scaled when the field and the magnetization were normalized by the characteristic scaling factors H(T)H^\ast(T) and M(T)M^\ast(T), respectively. This feature suggests that the pinning mechanism determining the hysteresis in M(H)M(H) is unique below T=TcT=T_c.Comment: 4pages and 4 figures. Phys. Rev. B (accepted

    An Optical Readout TPC (O-TPC) for Studies in Nuclear Astrophysics With Gamma-Ray Beams at HIgS

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    We report on the construction, tests, calibrations and commissioning of an Optical Readout Time Projection Chamber (O-TPC) detector operating with a CO2(80%) + N2(20%) gas mixture at 100 and 150 Torr. It was designed to measure the cross sections of several key nuclear reactions involved in stellar evolution. In particular, a study of the rate of formation of oxygen and carbon during the process of helium burning will be performed by exposing the chamber gas to intense nearly mono-energetic gamma-ray beams at the High Intensity Gamma Source (HIgS) facility. The O-TPC has a sensitive target-drift volume of 30x30x21 cm^3. Ionization electrons drift towards a double parallel grid avalanche multiplier, yielding charge multiplication and light emission. Avalanche induced photons from N2 emission are collected, intensified and recorded with a Charge Coupled Device (CCD) camera, providing two-dimensional track images. The event's time projection (third coordinate) and the deposited energy are recorded by photomultipliers and by the TPC charge-signal, respectively. A dedicated VME-based data acquisition system and associated data analysis tools were developed to record and analyze these data. The O-TPC has been tested and calibrated with 3.183 MeV alpha-particles emitted by a 148Gd source placed within its volume with a measured energy resolution of 3.0%. Tracks of alpha and 12C particles from the dissociation of 16O and of three alpha-particles from the dissociation of 12C have been measured during initial in-beam test experiments performed at the HIgS facility at Duke University. The full detection system and its performance are described and the results of the preliminary in-beam test experiments are reported.Comment: Supported by the Richard F. Goodman Yale-Weizmann Exchange Program, ACWIS, NY, and USDOE grant Numbers: DE-FG02-94ER40870 and DE-FG02-97ER4103

    Changes in the ceIl membrane of Lactobacillus bulgaricus during storage following freeze-drying

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    The mechanism of inactivation of freeze-dried Lactobacillus bulgaricus during storage in maltodextrin under controlled humidity was investigated. Evidence is presented supporting the hypothesis that membrane damage occurs during storage. A study on the lipid composition of the cells by gas chromatography showed a decrease in the unsaturated and saturated fatty acid content of the cell. Further evidence indicating membrane damage includes a decrease in membrane bound proton-translocating ATPase activity

    Grain Surface Models and Data for Astrochemistry

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    AbstractThe cross-disciplinary field of astrochemistry exists to understand the formation, destruction, and survival of molecules in astrophysical environments. Molecules in space are synthesized via a large variety of gas-phase reactions, and reactions on dust-grain surfaces, where the surface acts as a catalyst. A broad consensus has been reached in the astrochemistry community on how to suitably treat gas-phase processes in models, and also on how to present the necessary reaction data in databases; however, no such consensus has yet been reached for grain-surface processes. A team of ∼25 experts covering observational, laboratory and theoretical (astro)chemistry met in summer of 2014 at the Lorentz Center in Leiden with the aim to provide solutions for this problem and to review the current state-of-the-art of grain surface models, both in terms of technical implementation into models as well as the most up-to-date information available from experiments and chemical computations. This review builds on the results of this workshop and gives an outlook for future directions

    Ergonomics and sustainability: Towards and embrace of complexity and emergence

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    Technology offers a promising route to a sustainable future, and ergonomics can serve a vital role. The argument of this article is that the lasting success of sustainability initiatives in ergonomics hinges on an examination of ergonomics' own epistemology and ethics. The epistemology of ergonomics is fundamentally empiricist and positivist. This places practical constraints on its ability to address important issues such as sustainability, emergence and complexity. The implicit ethical position of ergonomics is one of neutrality, and its positivist epistemology generally puts value-laden questions outside the parameters of what it sees as scientific practice. We argue, by contrast, that a discipline that deals with both technology and human beings cannot avoid engaging with questions of complexity and emergence and seeking innovative ways of addressing these issues.No Full Tex

    Pion, kaon, proton and anti-proton transverse momentum distributions from p+p and d+Au collisions at sNN=200\sqrt{s_{NN}} = 200 GeV

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    Identified mid-rapidity particle spectra of π±\pi^{\pm}, K±K^{\pm}, and p(pˉ)p(\bar{p}) from 200 GeV p+p and d+Au collisions are reported. A time-of-flight detector based on multi-gap resistive plate chamber technology is used for particle identification. The particle-species dependence of the Cronin effect is observed to be significantly smaller than that at lower energies. The ratio of the nuclear modification factor (RdAuR_{dAu}) between protons (p+pˉ)(p+\bar{p}) and charged hadrons (hh) in the transverse momentum range 1.2<pT<3.01.2<{p_{T}}<3.0 GeV/c is measured to be 1.19±0.051.19\pm0.05(stat)±0.03\pm0.03(syst) in minimum-bias collisions and shows little centrality dependence. The yield ratio of (p+pˉ)/h(p+\bar{p})/h in minimum-bias d+Au collisions is found to be a factor of 2 lower than that in Au+Au collisions, indicating that the Cronin effect alone is not enough to account for the relative baryon enhancement observed in heavy ion collisions at RHIC.Comment: 6 pages, 4 figures, 1 table. We extended the pion spectra from transverse momentum 1.8 GeV/c to 3. GeV/
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