Computer-assisted ex vivo, normothermic small bowel perfusion

Background: In the present study, a technique for computer-assisted, normothermic, oxygenated, ex vivo, recirculating small bowel perfusion was established as a tool to investigate organ pretreatment protocols and ischemia/reperfusion phenomena. A prerequisite for the desired setup was an organ chamber for ex vivo perfusion and the use of syngeneic whole blood as perfusate. Methods: The entire small bowel was harvested from Lewis rats and perfused in an organ chamber ex vivo for at least 2 h. The temperature was kept at 37 degrees C in a water bath. Three experimental groups were explored, characterized by different perfusion solutions. The basic perfusate consisted of syngeneic whole blood diluted with either NaCl, Krebs' solution or Krebs' solution and norepinephrine to a hematocrit of 30%. In addition, in each group l-glutamine was administered intraluminally. The desired perfusion pressure was 100 mm Hg which was kept constant with a computer-assisted data acquisition software, which measured an-line pressure, oxygenation, flow, temperature and pH and adjusted the pressure by changing the flow via a peristaltic pump. The viability of the preparation was tested by measuring oxygen consumption and maltose absorption, which requires intact enzymes of the mucosal brush border to break down maltose into glucose. Results: Organ perfusion in group 1 (dilution with NaCl) revealed problems such as hypersecretion into the bowel lumen, low vascular resistance and no maltose uptake. In contrast a viable organ could be demonstrated using Krebs' solution as dilution solution. The addition of norepinephrine led to an improved perfusion over the entire perfusion period. Maltose absorption was comparable to tests conducted with native small bower. Oxygen consumption was stable during the 2-hour perfusion period. Conclusions: The ex vivo perfusion system established enables small bowel perfusion for at least 2 h. The viability of the graft could be demonstrated. The perfusion time achieved is sufficient to study leukocyte/lymphocyte interaction with the endothelium of the graft vessels. In addition, a viable small bowel, after 2 h of ex vivo perfusion, facilitates testing of pretreatment protocols for the reduction of the immunogenicity of small bowel allografts. Copyright (C) 2000 S. Karger AG, Basel

The Optical Transient Search in the Bamberg Southern Sky Survey: Preliminary Results

A large fraction of gamma-ray bursts temporarily emit optical light, i.e. optical afterglows and optical transients. So far, optical transients have only been detected after related gamma-ray satellite detection. However, taking into account their optical magnitudes at maximum light, these objects should be detectable in various historical and recent optical surveys, including the photographic sky patrol. Here we report on an extended study based on blink-comparison of 5004 Bamberg Observatory Southern Sky Patrol Plates performed within a student high school project (Jugend Forscht)

The significance of motivation in student-centred learning : a reflective case study

The theoretical underpinnings of student-centred learning suggest motivation to be an integral component. However, lack of clarification of what is involved in motivation in education often results in unchallenged assumptions that fail to recognise that what motivates some students may alienate others. This case study, using socio-cognitive motivational theory to analyse previously collected data, derives three fuzzy propositions which, collectively, suggest that motivation interacts with the whole cycle of episodes in the teachinglearning process. It argues that the development of the higherlevel cognitive competencies that are implied by the term, student-centred learning, must integrate motivational constructs such as goal orientation, volition, interest and attributions into pedagogical practices

The impact of climate change on incomes and convergence in Africa

© 2019 Elsevier Ltd Climate change is projected to detrimentally affect African countries’ economic development, while income inequalities across economies is among the highest on the planet. However, it is projected that income levels would converge on the continent. Hitherto there is limited evidence on how climate change could affect projected income convergence, accelerating, slowing down, or even reversing this process. Here, we analyze convergence considering climate-change damages, by employing an economic model embedding the three dimensions of risks at the country-level: exposure, vulnerability and hazards. The results show (1) with historical mean climate-induced losses between 10 and 15 percent of GDP per capita growth, the majority of African economies are poorly adapted to their current climatic conditions, (2) Western and Eastern African countries are projected to be the most affected countries on the continent and (3) As a consequence of these heightened impacts on a number of countries, inequalities between countries are projected to widen in the high warming scenario compared to inequalities in the low and without warming scenarios. To mitigate the impacts of economic development and inequalities across countries, we stress (1) the importance of mitigation ambition and Africa's leadership in keeping global mean temperature increase below 1.5 °C, (2) the need to address the current adaptation deficit as soon as possible, (3) the necessity to integrate quantitatively climate risks in economic and development planning and finally (4) we advocate for the generalization of a special treatment for the most vulnerable countries to access climate-related finance. The analysis raises issues on the ability of African countries to reach their SDGs targets and the potential increasing risk of instability, migration across African countries, of decreased trade and economic cooperation opportunities as a consequence of climate change – exacerbating its negative consequences

The impact of climate change on incomes and convergence in Africa

© 2019 Elsevier Ltd Climate change is projected to detrimentally affect African countries’ economic development, while income inequalities across economies is among the highest on the planet. However, it is projected that income levels would converge on the continent. Hitherto there is limited evidence on how climate change could affect projected income convergence, accelerating, slowing down, or even reversing this process. Here, we analyze convergence considering climate-change damages, by employing an economic model embedding the three dimensions of risks at the country-level: exposure, vulnerability and hazards. The results show (1) with historical mean climate-induced losses between 10 and 15 percent of GDP per capita growth, the majority of African economies are poorly adapted to their current climatic conditions, (2) Western and Eastern African countries are projected to be the most affected countries on the continent and (3) As a consequence of these heightened impacts on a number of countries, inequalities between countries are projected to widen in the high warming scenario compared to inequalities in the low and without warming scenarios. To mitigate the impacts of economic development and inequalities across countries, we stress (1) the importance of mitigation ambition and Africa's leadership in keeping global mean temperature increase below 1.5 °C, (2) the need to address the current adaptation deficit as soon as possible, (3) the necessity to integrate quantitatively climate risks in economic and development planning and finally (4) we advocate for the generalization of a special treatment for the most vulnerable countries to access climate-related finance. The analysis raises issues on the ability of African countries to reach their SDGs targets and the potential increasing risk of instability, migration across African countries, of decreased trade and economic cooperation opportunities as a consequence of climate change – exacerbating its negative consequences

High precision measurement of the associated strangeness production in proton proton interactions

A new high precision measurement of the reaction pp -> pK+Lambda at a beam momentum of 2.95 GeV/c with more than 200,000 analyzed events allows a detailed analysis of differential observables and their inter-dependencies. Correlations of the angular distributions with momenta are examined. The invariant mass distributions are compared for different regions in the Dalitz plots. The cusp structure at the N Sigma threshold is described with the Flatt\'e formalism and its variation in the Dalitz plot is analyzed.Comment: accepted for publication in Eur. Phys. J.

First Model-Independent Measurement of the Spin Triplet pΛp\Lambda Scattering Length from Final State Interaction in the p⃗p→pK+Λ\vec{p}p \rightarrow pK^{+}\Lambda Reaction

The $\vec{p}p \rightarrow pK^{+}\Lambda$ reaction has been measured with the COSY-TOF detector at a beam momentum of $2.7\,\mathrm{GeV}/c$. The polarized proton beam enables the measurement of the beam analyzing power by the asymmetry of the produced kaon ($A_N^{K}$). This observable allows the $p\Lambda$ spin triplet scattering length to be extracted for the first time model independently from the final-state interaction in the reaction. The obtained value is $a_{t} = (-2.55 ^{+0.72}_{-1.39} {}_{\textrm{stat.}} \pm 0.6_{\textrm{syst.}} \pm 0.3_{\textrm{theo.}})\mathrm{fm}$. This value is compatible with theoretical predictions and results from model-dependent analyses.Comment: Revised version as accepted for publication in PR

Range and extinction dynamics of the steppe bison in Siberia : A pattern-oriented modelling approach

Aim To determine the ecological processes and drivers of range collapse, population decline and eventual extinction of the steppe bison in Eurasia. Location Siberia. Time period Pleistocene and Holocene. Major taxa studied Steppe bison (Bison priscus). Methods We configured 110,000 spatially explicit population models (SEPMs) of climate-human-steppe bison interactions in Siberia, which we ran at generational time steps from 50,000 years before present. We used pattern-oriented modelling (POM) and fossil-based inferences of distribution and demographic change of steppe bison to identify which SEPMs adequately simulated important interactions between ecological processes and biological threats. These "best models" were then used to disentangle the mechanisms that were integral in the population decline and later extinction of the steppe bison in its last stronghold in Eurasia. Results Our continuous reconstructions of the range and extinction dynamics of steppe bison were able to reconcile inferences of spatio-temporal occurrence and the timing and location of extinction in Siberia based on hundreds of radiocarbon-dated steppe bison fossils. We showed that simulating the ecological pathway to extinction for steppe bison in Siberia in the early Holocene required very specific ecological niche constraints, demographic processes and a constrained synergy of climate and human hunting dynamics during the Pleistocene-Holocene transition. Main conclusions Ecological processes and drivers that caused ancient population declines of species can be reconstructed at high spatio-temporal resolutions using SEPMs and POM. Using this approach, we found that climatic change and hunting by humans are likely to have interacted with key ecological processes to cause the extinction of the steppe bison in its last refuge in Eurasia.Peer reviewe

Self-Reported Risks for Multiple-Drug Resistance among New Tuberculosis Cases: Implications for Drug Susceptibility Screening and Treatment

Multiple drug-resistance in new tuberculosis (TB) cases accounts for the majority of all multiple drug-resistant TB (MDR-TB) worldwide. Effective control requires determining which new TB patients should be tested for MDR disease, yet the effectiveness of global screening recommendations of high-risk groups is unknown.Sixty MDR-TB cases with no history of previous TB treatment, 80 drug-sensitive TB and 80 community-based controls were recruited in Lima, Peru between August and December, 2008 to investigate whether recommended screening practices identify individuals presenting with MDR-TB. Odd ratios (OR) and 95% confidence intervals (CI) were calculated using logistic regression to study the association of potential risk factors with case/control variables.MDR-TB cases did not differ from drug-sensitive TB and community controls in rates of human immunodeficiency virus infection, reported hospital or prison visits in the 3 years prior to diagnosis. MDR-TB cases were more likely than drug-sensitive TB controls to have had a recent MDR-TB household contact (OR 4.66, (95% CI 1.56–13.87)); however, only 15 cases (28.3%) reported this exposure. In multivariate modeling, recent TB household contact, but not contact with an MDR-TB case, remained predictive of MDR-TB, OR 7.47, (95% CI 1.91–29.3). Living with a partner rather than parents was associated with a lower risk of MDR-TB, OR 0.15, (95% CI 0.04–0.51).Targeted drug susceptibility testing (DST) linked to reported MDR-TB contact or other high-risk exposures does not identify the majority of new TB cases with MDR disease in Lima where it is endemic. All new TB cases should be screened with DST to identify MDR patients. These findings are likely applicable to other regions with endemic MDR-TB

Improved study of a possible Theta+ production in the pp -> p K0 sigma+ reaction with the COSY-TOF spectrometer

The pp -> p K0 Sigma+ reaction was investigated with the TOF spectrometer at COSY at 3.059 GeV/c incident beam momentum. The main objective was to clarify whether or not a narrow exotic S = +1 resnance, the Theta+ pentaquark, is populated at 1.53 GeV/c2 in the K0 p subsystem with a data sample of much higher statistical significance compared to the previously reported data in this channel. An analysis of these data does not confirm the existence of the Theta+ pentaquark. This is expressed as an upper limit for the cross section sigma (pp -> p K0 Sigma+) < 0.15 microbarn at the 95 percent confidence level.Comment: 11 pages, 5 figure