Observations of HI Absorbing Gas in Compact Radio Sources at Cosmological Redshifts

We present an overview of the occurrence and properties of atomic gas associated with compact radio sources at redshifts up to z=0.85. Searches for HI 21cm absorption were made with the Westerbork Synthesis Radio Telescope at UHF-high frequencies (725-1200 MHz). Detections were obtained for 19 of the 57 sources with usable spectra (33%). We have found a large range in line depths, from tau=0.16 to tau<=0.001. There is a substantial variety of line profiles, including Gaussians of less than 10km/s, to more typically 150km/s, as well as irregular and multi-peaked absorption profiles, sometimes spanning several hundred km/s. Assuming uniform coverage of the entire radio source, we obtain column depths of atomic gas between 1e19 and 3.3e21(Tsp/100K)(1/f)cm^(-2). There is evidence for significant gas motions, but in contrast to earlier results at low redshift, there are many sources in which the HI velocity is substantially negative (up to v=-1420km/s) with respect to the optical redshift, suggesting that in these sources the atomic gas, rather than falling into the centre, may be be flowing out, interacting with the jets, or rotating around the nucleus.Comment: 10 pages, accepted for publication in A&

Carotid atherosclerotic plaque matrix metalloproteinase-12-positive macrophage subpopulation predicts adverse outcome after endarterectomy

BACKGROUND: Matrix metalloproteinase-12 (MMP-12) promotes atherosclerosis in animal models. MMP-12 is expressed in only a subset of foam-cell macrophages (FCMs) in human plaques. We investigated whether the prevalence of this MMP-12–expressing subpopulation is a prognostic indicator of adverse outcome in patients after carotid endarterectomy (CEA). METHODS AND RESULTS: Serial sections of culprit lesions from 236 patients who underwent CEA and had undergone 3 years of clinical follow-up were stained immunocytochemically for MMP-12 and for CD68, and the MMP-12/CD68 ratio was used to quantify the MMP-12–expressing subpopulation. A high MMP-12/CD68 ratio correlated with a high content of lipid and total macrophages and a low content of vascular smooth muscle cells, as well as with MMP-8 (R=0.211, P=0.001), MMP-9 (R=0.251, P<0.001), and cleaved caspase-3 (R=0.142, P=0.036) activity measured in a neighboring segment. Dual immunohistochemical examination confirmed the location of MMP-12 in a subpopulation of MMP-8– and MMP-9–positive FCMs, whereas all apoptotic FCMs were MMP-12 positive. Patients who yielded plaques within the highest quartile compared with the lowest quartile of MMP-12/CD68 ratio had a 2.4-fold (hazard ratio, 2.4; 95% CI, 1.1- to 5.1-fold; adjusted P=0.027) increased risk of major adverse cardiovascular event and a 3.4-fold (3.4; 1.2- to 9.6-fold, P=0.024) increased risk for stroke. CONCLUSIONS: The prevalence of an MMP-12–positive subset of FCMs is a prognostic marker for adverse clinical outcome after CEA

First discoveries of z similar to 6 quasars with the Kilo-Degree Survey and VISTA Kilo-Degree Infrared Galaxy survey

This work is financially supported by the Netherlands Research School for Astronomy (NOVA) and Target. Target is supported by Samenwerkingsverband Noord Nederland, European fund for regional development, Dutch Ministry of economic affairs, Pieken in de Delta, Provinces of Groningen and Drenthe

Caveolin-1 Influences Vascular Protease Activity and Is a Potential Stabilizing Factor in Human Atherosclerotic Disease

Caveolin-1 (Cav-1) is a regulatory protein of the arterial wall, but its role in human atherosclerosis remains unknown. We have studied the relationships between Cav-1 abundance, atherosclerotic plaque characteristics and clinical manisfestations of atherosclerotic disease.We determined Cav-1 expression by western blotting in atherosclerotic plaques harvested from 378 subjects that underwent carotid endarterectomy. Cav-1 levels were significantly lower in carotid plaques than non-atherosclerotic vascular specimens. Low Cav-1 expression was associated with features of plaque instability such as large lipid core, thrombus formation, macrophage infiltration, high IL-6, IL-8 levels and elevated MMP-9 activity. Clinically, a down-regulation of Cav-1 was observed in plaques obtained from men, patients with a history of myocardial infarction and restenotic lesions. Cav-1 levels above the median were associated with absence of new vascular events within 30 days after surgery [0% vs. 4%] and a trend towards lower incidence of new cardiovascular events during longer follow-up. Consistent with these clinical data, Cav-1 null mice revealed elevated intimal hyperplasia response following arterial injury that was significantly attenuated after MMP inhibition. Recombinant peptides mimicking Cav-1 scaffolding domain (Cavtratin) reduced gelatinase activity in cultured porcine arteries and impaired MMP-9 activity and COX-2 in LPS-challenged macrophages. Administration of Cavtratin strongly impaired flow-induced expansive remodeling in mice.This is the first study that identifies Cav-1 as a novel potential stabilizing factor in human atherosclerosis. Our findings support the hypothesis that local down-regulation of Cav-1 in atherosclerotic lesions contributes to plaque formation and/or instability accelerating the occurrence of adverse clinical outcomes. Therefore, given the large number of patients studied, we believe that Cav-1 may be considered as a novel target in the prevention of human atherosclerotic disease and the loss of Cav-1 may be a novel biomarker of vulnerable plaque with prognostic value

Dust tori in radio galaxies

We investigate the validity of the quasar - radio galaxy unification scenario and detect dust tori within radio galaxies of various types. Using VISIR on the VLT, we acquired sub-arcsecond (~0.40") resolution N-band images, at a wavelength of 11.85 micron, of the nuclei of a sample of 27 radio galaxies of four types in the redshift range z=0.006-0.156. The sample consists of 8 edge-darkened, low-power Fanaroff-Riley class I (FR-I) radio galaxies, 6 edge-brightened, class II (FR-II) radio galaxies displaying low-excitation optical emission, 7 FR-IIs displaying high-excitation optical emission, and 6 FR-II broad emission line radio galaxies. Out of the sample of 27 objects, 10 nuclei are detected and several have constraining non-detections at sensitivities of 7 mJy, the limiting flux a point source has when detected with a signal-to-noise ratio of 10 in one hour of source integration. On the basis of the core spectral energy distributions of this sample we find clear indications that many FR-I and several low-excitation FR-II radio galaxies do not contain warm dust tori. At least 57+-19 percent of the high-excitation FR-IIs and almost all broad line radio galaxies display excess infrared emission, which must be attributed to warm dust reradiating accretion activity. The FR-I and low-excitation FR-II galaxies all possess low efficiencies, calculated as the ratio of bolometric and Eddington luminosity log (L_bol/L_Edd) < -3. This suggests that thick tori are absent at low accretion rates and/or low efficiencies. We argue that the unification viewing angle range 0-45 degrees of quasars should be increased to ~60 degrees, at least at lower luminosities.Comment: 17 pages, 9 figures, published in Astronomy and Astrophysics. Version 2 matches published version

Novel Protocol for the Chemical Synthesis of Crustacean Hyperglycemic Hormone Analogues — An Efficient Experimental Tool for Studying Their Functions

The crustacean Hyperglycemic Hormone (cHH) is present in many decapods in different isoforms, whose specific biological functions are still poorly understood. Here we report on the first chemical synthesis of three distinct isoforms of the cHH of Astacus leptodactylus carried out by solid phase peptide synthesis coupled to native chemical ligation. The synthetic 72 amino acid long peptide amides, containing L- or D-Phe3 and (Glp1, D-Phe3) were tested for their biological activity by means of homologous in vivo bioassays. The hyperglycemic activity of the D-isoforms was significantly higher than that of the L-isoform, while the presence of the N-terminal Glp residue had no influence on the peptide activity. The results show that the presence of D-Phe3 modifies the cHH functionality, contributing to the diversification of the hormone pool