Clinical Experience of Luminescent Diagnostics of Precancerous Diseases and Cervical Cancer

The article summarizes the experience of using luminescent diagnostics with the use of ytterbium porphyrin complexes in gynecology and oncology. A pharmaceutical composition based on the Yb complex of 2,4-dimethoxyhematoporphyrin IX was used as the luminescent markers within the infrared range. The determination of luminescence characteristics (luminescence intensity) was carried out using a laserfiber fluorimeter in the range of 900-1100 nm. A new method for diagnosis of cervical disease has been proposed. The method of luminescent diagnostics allows to conduct a survey of a large number of patients in a short time. The method of luminescent diagnostics using the ytterbium complexes of porphyrins is not invasive. The method can be used as a screening. Differences between normal and pathologically altered cervical tissue have been identified and differences between pathological changes in the cervix HSIL (CIN II, CIN III) and cervical cancer are reliable. Keywords: Cervical cancer, squamous cell carcinoma, diagnosis of cervical cancer, squamous intraepithelial lesions of high grade – HSIL, luminescent diagnostics, luminescing in the near infrared (NIR) spectral region, porphyrins, ytterbium complexes of porphyrins

Diagnosis of Cervical Cancer Using Raman Spectroscopy

The aim of the study was to develop a method of detecting cervical cancer using Raman spectroscopy in the examination of biopsy and surgical material. Significant differences in the spectral characteristics between the tissues of the intact cervix and tissues with squamous cell carcinoma of the cervix have been revealed. Intensity of fluorescence in cervical cancer was higher than in intact cervical tissue.     Keywords: cervical cancer, squamous cell carcinoma, diagnosis of cervical cancer, fluorescence in cervical cancer, Raman spectroscopy for the diagnosis of cervical cance

Simulation of the processes of plasma modification and vacuum metallization of polymer materials by the method of molecular dynamics

The article presents the results of the development of molecular dynamics models of modifications of polypropylene (PP) material in the plasma of a radio-frequency (RF) discharge and a copper coating deposit by magnetron sputtering on the surface of a polyethylene (PE) material. The model of the RF plasma modification process describes changes in the surface layers of the PP material upon interaction with low-energy plasma ions: the nature of the breaking of covalent bonds in macromolecules, the chemical composition of sputtered particles, and changes in the ordering of the supramolecular structure. The model of the vacuum metallization process describes the processes of the introduction of metal atoms into the polymer structure, the change in the conformation of macromolecules, the formation of macroradicals with uncompensated chemical bonds, and the formation of an interfacial layer between the polymer and the metal coating

RESEARCH OF THE INFLUENCE OF HIGH-FREQUENCY PLASMA TREATMENT ON THE ABSORPTION TIME OF WATER IN BASALT TISSUE

The influence of different high-frequency plasma modes on the absorption time of water in basalt tissue has been investigated. It has been established that, depending on the parameters of high-frequency plasma treatment, it is possible to achieve both hydrophobicity and increased hydrophilicity of basaltic tissues

STUDY OF THE EFFECT OF HIGH-FREQUENCY CAPACITIVE PLASMA TREATMENT ON CAPILLARY PROPERTIES OF BASALT FABRICS

Were studied the capillary of basalt fiber after low-temperature plasma treatment in radio-frequency capacitive discharge. It was established experimentally increase in the height of the capillary rise of water for the treated sample

Metabolic dysregulation in vitamin E and carnitine shuttle energy mechanisms associate with human frailty

Global ageing poses a substantial economic burden on health and social care costs. Enabling a greater proportion of older people to stay healthy for longer is key to the future sustainability of health, social and economic policy. Frailty and associated decrease in resilience plays a central role in poor health in later life. In this study, we present a population level assessment of the metabolic phenotype associated with frailty. Analysis of serum from 1191 older individuals (aged between 56 and 84 years old) and subsequent longitudinal validation (on 786 subjects) was carried out using liquid and gas chromatography-mass spectrometry metabolomics and stratified across a frailty index designed to quantitatively summarize vulnerability. Through multivariate regression and network modelling and mROC modeling we identified 12 significant metabolites (including three tocotrienols and six carnitines) that differentiate frail and non-frail phenotypes. Our study provides evidence that the dysregulation of carnitine shuttle and vitamin E pathways play a role in the risk of frailty

Measures of frailty in population-based studies: An overview

Although research productivity in the field of frailty has risen exponentially in recent years, there remains a lack of consensus regarding the measurement of this syndrome. This overview offers three services: first, we provide a comprehensive catalogue of current frailty measures; second, we evaluate their reliability and validity; third, we report on their popularity of use

Ecological communications of modern students (experience of the Moscow automotive-road state technical university)

The article presents the results of the analysis of the process of expansion and diversity of communication channels in the university environment, which is relevant from the standpoint of achieving the goals of sustainable development in the field of education. It is shown that one of the most accessible and open communication channels, in which modern students are actively involved, is the channel of environmental communications. They have many manifestations, which corresponds to the most exceptionally wide ecological space, reflecting both individual and collective interests, features of a narrow professional attitude to nature and its civilizational understanding. A significant part of the disciplines studied in higher education contributes to the formation of students' environmental competencies. The specificity of the ecological approach to education and upbringing contributes to involvement of students in activities for the nature protection. The regularities of environmental communications functioning can be traced on the example of environmental education carried out at the Moscow Automobile Road State Technical University (MADI)

The NH2-Terminal Region of Rabbit CYP2E1 Is Not Essential for Interaction with NADPH-Cytochrome P450 Reductase

Recently we reported that electrostatic forces interfere with the formation of the NADPH-cytochrome P450 reductase/cytochrome P450 electron transfer complex and suggested that this complex is formed by the attraction of the complementary hydrophobic patches (Voznesensky, A., and Schenkman,J.(1994) 269 J.Biol.Chem, 15724-1573). In this report we evaluate the role of the NH2-terminal hydrophobic region of CYP2E1 in the interaction with the reductase by comparing ionic strength dependence of the reduction of the full-length and truncated [Delta]3-29 CYP2E1. Increasing ionic strength stimulates reduction of both full-length and truncated CYP2E1. The neutralization of electrostatic interactions by increasing ionic strength revealed no impairment of the [Delta]3-29 CYP2E1 reduction compared to that of the full-length CYP2E1, indicating that the NH2-terminal region is not essential for the interaction of the cytochrome with the reductase.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31380/3/0000293.pd

Simulation of the processes of plasma modification and vacuum metallization of polymer materials by the method of molecular dynamics

The article presents the results of the development of molecular dynamics models of modifications of polypropylene (PP) material in the plasma of a radio-frequency (RF) discharge and a copper coating deposit by magnetron sputtering on the surface of a polyethylene (PE) material. The model of the RF plasma modification process describes changes in the surface layers of the PP material upon interaction with low-energy plasma ions: the nature of the breaking of covalent bonds in macromolecules, the chemical composition of sputtered particles, and changes in the ordering of the supramolecular structure. The model of the vacuum metallization process describes the processes of the introduction of metal atoms into the polymer structure, the change in the conformation of macromolecules, the formation of macroradicals with uncompensated chemical bonds, and the formation of an interfacial layer between the polymer and the metal coating