Vila Flores: Um Processo Arquitetônico

The article presents a discussion about the architectural process of Vila Flores, in Porto Alegre. From the understanding of the paradigms arising from contemporaneity, the article, besides describing the transformations of Vila Flores throughout history, identifies in the same concepts of open source, low-up operation and systemic organization. In conclusion, the article understands Vila Flores as an example of an architectural process based on the issues imposed by contemporaneity.El artículo presenta una discusión sobre el proceso arquitectónico del Vila Flores, en Porto Alegre. A partir del entendimiento de los paradigmas que vienen de la contemporaneidad, el artículo, además de describir las transformaciones de Vila Flores a lo largo de la historia, identifica en el mismo concepto de código abierto, de operación de abajo hacia arriba y de organización sistémica. En conclusión, el artículo entiende el Vila Flores como un ejemplo de proceso arquitectónico pautado por las cuestiones impuestas por la contemporaneidad

VILA FLORES: UM PROCESSO ARQUITETÔNICO

The article presents a discussion about the architectural process of Vila Flores, in Porto Alegre. From the understanding of the paradigms arising from contemporaneity, the article, besides describing the transformations of Vila Flores throughout history, identifies in the same concepts of open source, low-up operation and systemic organization. In conclusion, the article understands Vila Flores as an example of an architectural process based on the issues imposed by contemporaneity.El artículo presenta una discusión sobre el proceso arquitectónico del Vila Flores, en Porto Alegre. A partir del entendimiento de los paradigmas que vienen de la contemporaneidad, el artículo, además de describir las transformaciones de Vila Flores a lo largo de la historia, identifica en el mismo concepto de código abierto, de operación de abajo hacia arriba y de organización sistémica. En conclusión, el artículo entiende el Vila Flores como un ejemplo de proceso arquitectónico pautado por las cuestiones impuestas por la contemporaneidad

O Museu do Pão e o Caminho dos Moinhos

The article presents the case study of the Museu do Pão as a strategy of the regional place production through the architectural intervention. For that, the concepts of positive metastasis, acupuncture and placemaking are discussed, relating territorial scale and memory through the rearchitecture. It starts from the territorial scale, presenting, first, a cultural panorama of the Caminho Moinhos and identifying the Museu do Pão as a first initiative in the way of the conservation of the cultural memory.Key-words: Caminho dos Moinhos, Museu do Pão, positive metastasis, acupunture, placemakingO artigo apresenta, a partir do estudo de caso do Museu do Pão, a estratégia de produção do lugar regional por meio da intervenção arquitetônica. Para tanto, abordam-se os conceitos de metástase positiva, acupuntura e placemaking, relacionando a escala territorial e a memória através da intervenção arquitetônica. Parte-se da escala territorial, apresentando, primeiramente, um panorama cultural do Caminho dos Moinhos e identificando o Museu do Pão como uma primeira iniciativa no caminho da conservação da memória cultural.Palavras-chave: Caminho dos Moinhos, Museu do Pão, metástase positiva, acupuntura, placemaking

Exceptional Clinical Response to Alectinib in Pancreatic Acinar Cell Carcinoma With a Novel ALK-KANK4 Gene Fusion

This case report corroborates the indication for patients affected by these rare tumors to be treated in higher- volume cancer centers to provide patients with more ex- perience and access to NGS diagnostic resources. Most importantly, it raises enthusiasm and hope toward precision medicine and tailored treatments for patients with PACC

Specific Detection of Cytokeratin 20-Positive Cells in Blood of Colorectal and Breast Cancer Patients by a High Sensitivity Real-Time Reverse Transcriptase-Polymerase Chain Reaction Method

A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method for detection of cytokeratin 20-positive cells in blood characterized by two novel features was developed and tested on 99 patients with colorectal cancer, 110 with breast cancer, and 150 healthy subjects. To optimize the specificity and sensitivity of the method, two novel features were used. First, a primer overlapping two adjacent exons was generated to inhibit nonspecific amplification both in healthy donors and cancer patients; second, a non-end-point first-round amplification was used to increase sensitivity. The number of first-round cycles was chosen to reach the highest level of sensitivity while conserving quantitative characteristics. PCR efficiency increased from 88.9% in single-round RT-PCR to 99.0% in nested real-time RT-PCR. To establish sensitivity and specificity of the method, HT29 cells were serially diluted with normal blood. Detection limit improved from 100 HT29 cells (single-round RT-PCR) to 1 to 10 cells (nested real-time RT-PCR) per 3 ml of whole blood. None of the healthy subjects was positive, whereas 22 and 29% of all colorectal and breast cancer patients, respectively, had cytokeratin 20 cell equivalents in blood. The association between cytokeratin 20 cell equivalents and metastasis was statistically significant for breast (P = 0.026) but not colorectal cancer patients (P = 0.361). Negativity of all 150 healthy controls examined confers diagnostic potential to the method