Lifetime measurement of the metastable 3d 2D5/2 state in the 40Ca+ ion using the shelving technique on a few-ion string

We present a measurement of the lifetime of the metastable 3d 2D5/2 state in the 40Ca+ ion, using the so-called shelving technique on a string of five Doppler laser-cooled ions in a linear Paul trap. A detailed account of the data analysis is given, and systematic effects due to unwanted excitation processes and collisions with background gas atoms are discussed and estimated. From a total of 6805 shelving events, we obtain a lifetime tau=1149+/-14(stat.)+/-4(sys.)ms, a result which is in agreement with the most recent measurements.Comment: 10 pages, 7 figures. Submitted for publicatio

Core handling and processing for the WAIS Divide ice-core project

On 1 December 2011 the West Antarctic Ice Sheet (WAIS) Divide ice-core project reached its final depth of 3405 m. The WAIS Divide ice core is not only the longest US ice core to date, but is also the highest-quality deep ice core, including ice from the brittle ice zone, that the US has ever recovered. The methods used at WAIS Divide to handle and log the drilled ice, the procedures used to safely retrograde the ice back to the US National Ice Core Laboratory (NICL) and the methods used to process and sample the ice at the NICL are described and discussed

Water solubility in aluminosilicate melts of haplogranite composition at 2 kbar

The compositional dependence of H2O solubility was investigated at 2 kbar and 800°C in haplogranite melts (system SiO2---1bNaAlSi3O8---1bKAlSi3O8 or Qz---1bAb---1bOr). The sixteen investigated compositions contained 25, 35 or 45 wt.% normative Qz and various Ab/(Ab+Or) ratios (0.15–0.92). Starting solid materials were anhydrous bubble-free glasses to which 10 wt.% H2O was added. The H2O contents of the isobarically quenched melts (glasses) were measured by Karl-Fischer titration. The results show that H2O solubility in aluminosilicate melts depends significantly upon anhydrous composition. The highest solubility values are obtained for the most Ab-rich melts. At a constant normative quartz content, the solubility of water decreases from 6.49 ± 0.20 wt.% H2O for a composition Qz35Ab60Or05 (normative composition expressed in wt.%) to 5.50 ± 0.15 wt.% H2O for a composition Qz35Ab10Or55. Along this join, the most significant changes are observed for Ab-rich melts whereas H2O solubility in Or-rich melts remains almost constant. The H2O solubility data imply that H2O is preferentially associated with the Ab component in aluminosilicate melts. Application of the results to natural granitic melts suggests that Na-rich, H2O-saturated melts may be significantly less viscous than H2O-saturated, K-rich melts. The temperature dependence of H2O solubility, investigated for composition Qz28Ab38Or34 at 2 kbar, is low. Increasing temperature from 750° to 1150°C only causes a decrease in H2O solubility from 6.00 to 5.41 wt.% H2O. These data are in agreement with previous data obtained for albite melts

Core handling and processing for the WAIS Divide ice-core project

On 1 December 2011 the West Antarctic Ice Sheet (WAIS) Divide ice-core project reached its final depth of 3405 m. The WAIS Divide ice core is not only the longest US ice core to date, but is also the highest-quality deep ice core, including ice from the brittle ice zone, that the US has ever recovered. The methods used at WAIS Divide to handle and log the drilled ice, the procedures used to safely retrograde the ice back to the US National Ice Core Laboratory (NICL) and the methods used to process and sample the ice at the NICL are described and discussed

Five millennia of surface temperatures and ice core bubble characteristics from the WAIS Divide deep core, West Antarctica

Bubble number densities from the West Antarctic Ice Sheet (WAIS) Divide deep core in West Antarctica record relatively stable temperatures during the middle Holocene followed by late Holocene cooling. We measured bubble number density, shape, size, and arrangement on new samples of the main WAIS Divide deep core WDC06A from similar to 580m to similar to 1600 depth. The bubble size, shape, and arrangement data confirm that the samples satisfy the requirements for temperature reconstructions. A small correction for cracks formed after core recovery allows extension of earlier work through the brittle ice zone, and a site-specific calibration reduces uncertainties. Using an independently constructed accumulation rate history and a steady state bubble number density model, we determined a temperature reconstruction that agrees closely with other independent estimates, showing a stable middle Holocene, followed by a cooling of similar to 1.25 degrees C in the late Holocene. Over the last similar to 5 millennia, accumulation has been higher during warmer times by similar to 12%degrees C-1, somewhat stronger than for thermodynamic control alone, suggesting dynamic processes

Continued deceleration of Whillans Ice Stream, West Antarctica

Author Posting. © American Geophysical Union, 2005. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 32 (2005): L22501, doi:10.1029/2005GL024319.Earlier observations indicated that Whillans Ice Stream slowed from 1973 to 1997. We collected new GPS observations of the ice stream's speed in 2003 and 2004. These data show that the ice stream is continuing to decelerate at rates of about 0.6%/yr2, with faster rates near the grounding line. Our data also indicate that the deceleration extends over the full width of the ice plain. Extrapolation of the deceleration trend suggests the ice stream could stagnate sometime between the middle of the 21st and 22nd Centuries.This work was supported by the National Science Foundation (NSF-OPP-0229659). IJ’s contribution was supported by the Cryospheric Sciences Program of NASA’s Earth Science Enterprise

Algorithm for backrub motions in protein design

Motivation: The Backrub is a small but kinematically efficient side-chain-coupled local backbone motion frequently observed in atomic-resolution crystal structures of proteins. A backrub shifts the Cα–Cβ orientation of a given side-chain by rigid-body dipeptide rotation plus smaller individual rotations of the two peptides, with virtually no change in the rest of the protein. Backrubs can therefore provide a biophysically realistic model of local backbone flexibility for structure-based protein design. Previously, however, backrub motions were applied via manual interactive model-building, so their incorporation into a protein design algorithm (a simultaneous search over mutation and backbone/side-chain conformation space) was infeasible

Biomass production of herbaceous energy crops in the United States: field trial results and yield potential maps from the multiyear regional feedstock partnership

Current knowledge of yield potential and best agronomic management practices for perennial bioenergy grasses is primarily derived from small-scale and short-term studies, yet these studies inform policy at the national scale. In an effort to learn more about how bioenergy grasses perform across multiple locations and years, the U.S. Department of Energy (US DOE)/Sun Grant Initiative Regional Feedstock Partnership was initiated in 2008. The objectives of the Feedstock Partnership were to (1) provide a wide range of information for feedstock selection (species choice) and management practice options for a variety of regions and (2) develop national maps of potential feedstock yield for each of the herbaceous species evaluated. The Feedstock Partnership expands our previous understanding of the bioenergy potential of switchgrass, Miscanthus, sorghum, energycane, and prairie mixtures on Conservation Reserve Program land by conducting long-term, replicated trials of each species at diverse environments in the U.S. Trials were initiated between 2008 and 2010 and completed between 2012 and 2015 depending on species. Field-scale plots were utilized for switchgrass and Conservation Reserve Program trials to use traditional agricultural machinery. This is important as we know that the smaller scale studies often overestimated yield potential of some of these species. Insufficient vegetative propagules of energycane and Miscanthus prohibited farm-scale trials of these species. The Feedstock Partnership studies also confirmed that environmental differences across years and across sites had a large impact on biomass production. Nitrogen application had variable effects across feedstocks, but some nitrogen fertilizer generally had a positive effect. National yield potential maps were developed using PRISM-ELM for each species in the Feedstock Partnership. This manuscript, with the accompanying supplemental data, will be useful in making decisions about feedstock selection as well as agronomic practices across a wide region of the country

Finding needles in haystacks:Linking scientific names, reference specimens and molecular data for Fungi

DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Reannotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi.The Intramural Research Programs of the National Center for Biotechnology Information, National Library of Medicine and the National Human Genome Research Institute, both at the National Institutes of Health.http://www.ncbi.nlm.nih.gov/bioproject/PRJNA177353am201