11 research outputs found
Fighting HIV/AIDS in a developing country: lessons from a small cohort from the largest Brazilian city
Not Applicabl
International multicenter observational study on assessment of ventilatory management during general anaesthesia for robotic surgery and its effects on postoperative pulmonary complication (AVATaR) : study protocol and statistical analysis plan
Introduction: Robotic-assisted surgery (RAS) has emerged as an alternative minimally invasive surgical option. Despite its growing applicability, the frequent need for pneumoperitoneum and Trendelenburg position could significantly affect respiratory mechanics during RAS. AVATaR is an international multicenter observational study aiming to assess the incidence of postoperative pulmonary complications (PPC), to characterise current practices of mechanical ventilation (MV) and to evaluate a possible association between ventilatory parameters and PPC in patients undergoing RAS.
Methods and analysis: AVATaR is an observational study of surgical patients undergoing MV for general anaesthesia for RAS. The primary outcome is the incidence of PPC during the first five postoperative days. Secondary outcomes include practice of MV, effect of surgical positioning on MV, effect of MV on clinical outcome and intraoperative complications.
Ethics and dissemination: This study was approved by the Institutional Review Board of the Hospital Israelita Albert Einstein. The study results will be published in peer-reviewed journals and disseminated at international conferences.
Trial registration number: NCT02989415; Pre-results
Association Between Cationic Liposomes And Low Molecular Weight Hyaluronic Acid
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential.311133083317Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [2011/19952-6, 2010/02203-8]CNPq [400796/2012-0
Antioxidant lifestyle, co-morbidities and quality of life empowerment concerning liver fibrosis
The assessment of liver fibrosis has gained importance since the progression of non-alcoholic fatty liver disease (NAFLD). Indeed, the description of the association between undetected liver fibrosis and lifestyle in terms of antioxidant habits, comorbidity and quality of life (QoL) domains may help in the characterization of subjects with NAFLD. A cross-sectional evaluation of (n = 116) consecutive patients from an Internal Medicine ambulatory evaluation was performed. Demographic data, lifestyle, co-morbidity, QoL (according to the SF-36 index) and analytical values to calculate the oxidative related Fibrosis-4 (FIB-4) index were recorded. The association between FIB-4 and co-morbidity, antioxidant habits in QoL was assessed in univariate analysis (p < 0.05) and confirmed in multivariable analysis for 4 of the 8 SF-36 categories: Physical QoL, Physical role, Social QoL and General QoL, as well as in the Physical summary of SF-36 (p < 0.05). Finally, interactions were assessed between co-morbidity, FIB-4 and antioxidant habits showed in the prediction of mean SF-36 (p < 0.01). Liver fibrosis assessed by the oxidative surrogate index FIB-4 is associated with the interaction between antioxidant lifestyle, co-morbidity and physical, social and general aspects of QoL in apparent liver disease-free individuals, generating a proof of concept for health empowerment and personalized medicine
Antioxidant lifestyle, co-morbidities and quality of life empowerment concerning liver fibrosis
The assessment of liver fibrosis has gained importance since the progression of non-alcoholic fatty liver disease (NAFLD). Indeed, the description of the association between undetected liver fibrosis and lifestyle in terms of antioxidant habits, comorbidity and quality of life (QoL) domains may help in the characterization of subjects with NAFLD. A cross-sectional evaluation of (n = 116) consecutive patients from an Internal Medicine ambulatory evaluation was performed. Demographic data, lifestyle, co-morbidity, QoL (according to the SF-36 index) and analytical values to calculate the oxidative related Fibrosis-4 (FIB-4) index were recorded. The association between FIB-4 and co-morbidity, antioxidant habits in QoL was assessed in univariate analysis (p < 0.05) and confirmed in multivariable analysis for 4 of the 8 SF-36 categories: Physical QoL, Physical role, Social QoL and General QoL, as well as in the Physical summary of SF-36 (p < 0.05). Finally, interactions were assessed between co-morbidity, FIB-4 and antioxidant habits showed in the prediction of mean SF-36 (p < 0.01). Liver fibrosis assessed by the oxidative surrogate index FIB-4 is associated with the interaction between antioxidant lifestyle, co-morbidity and physical, social and general aspects of QoL in apparent liver disease-free individuals, generating a proof of concept for health empowerment and personalized medicine
Association between cationic liposomes and low molecular weight hyaluronic acid
This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential311133083317CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP400796/2012-02011/19952-6; 2010/02203-
Floresta Sintá(c)tica: um recurso para avaliação
This work presents a study of the
association between low molecular
weight hyaluronic acid (16 kDa HA) and cationic liposomes composed
of egg phosphatidylcholine (EPC), 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane
(DOTAP). The cationic liposome/HA complexes were evaluated to determine
their mesoscopic structure, average size, zeta potential, and morphology
as a function of the amount of HA in the system. Small angle X-ray
scattering results revealed that neighboring cationic liposomes either
stick together after a partial coating of low concentration HA or
disperse completely in excess of HA, but they never assemble as multilamellar
vesicles. Cryo-transmission electron microscopy images confirm the
existence of unilamellar vesicles and large aggregates of unilamellar
vesicles for HA fractions up to 80% (w/w). High concentrations of
HA (> 20% w/w) proved to be efficient for coating extruded liposomes,
leading to particle complexes with sizes in the nanoscale range and
a negative zeta potential
Dietary Intake of Individual (Intrinsic and Added) Sugars and Food Sources from Spanish Children Aged One to <10 Years—Results from the EsNuPI Study
Currently, in Spain there are no studies assessing the intakes and sources of intrinsic
and added sugars by both children consuming standard milks and children regularly consuming
adapted milk formulas. Our goal was to evaluate current sugar intake levels (intrinsic and added)
and their major dietary sources within the EsNuPI study participants by applying two 24-h dietary
recalls that were completed by 1448 children (1 to <10 years) divided into two subsamples: One
“Spanish Reference Sample” (SRS) of the general population (n = 707) and another sample which
included children consuming adapted milks including follow-on milk, toddler’s or growing up milk
and fortified and enriched milks, here called “Adapted Milk Consumers Sample” (AMS) (n = 741).
Estimates of intrinsic and added sugar intakes from the Spanish EsNuPI population as well as the
adherence to recommendations varied notably according to age segment, but no major differences
between subsamples were found. Younger children (1 to <3 years) showed the highest added sugar
contribution to total energy intake (TEI) (SRS: 12.5% for boys and 11.7% for girls; AMS: 12.2% for
boys and 11.3% for girls) and the lowest adherence to recommendations set at <10% TEI (SRS: 27.4%
for boys and 37.2% for girls; AMS: 31.3% for boys and 34.7% for girls). Adherence increased with age but remains inadequate, with approximately one in two children from the older age segment (6 to
<10 years) exceeding the recommendations. Main food sources of intrinsic sugars for both subsamples
were milk and dairy products, fruits, vegetables and cereals, while for added sugars, these were milk
and dairy products (mainly yogurts), sugars and sweets (mainly sugary cocoa and nougat), bakery
products (mainly cookies) and cereals (mainly bread and wheat flour). However, for the AMS, the
groups milk and dairy products and cereals showed a significantly lower contribution to intrinsic
sugar intake but a significantly higher contribution to that of added sugars. These results demonstrate
that sugar intake and the adherence to recommendations in the studied population varied notably
according to age but not to the type of milk consumed. In addition, our results highlight the need
to monitor the consumption of added sugars by the infant population, as well as the need to make
efforts to facilitate this task, such as harmonizing the recommendations regarding free/added sugars
and the inclusion of information on their content on the nutritional labeling of products in order to
incorporate them into food composition databases