La "question" de la responsabilité dans l'écriture de presse

L'écriture de presse, qui se donne volontiers le leurre légitimant de l'impartialité étayée par une conception réductrice d'un langage transparent et instrumentalisable (Koren 1996, 2004), est en fait travaillée par les tensions de la subjectivisation et de l'objectivisation. Elle joue un rôle essentiel dans la circulation des discours, dans la construction d'un « miroir social » en constant devenir et met notamment en spectacle de manière privilégiée les paroles d'autrui à travers la constitution de sa propre mémoire intradiscursive (Moirand 2000). Les numéros 13 et 17 de Semen centrés sur l'analyse de discours et consacrés respectivement aux genres de la presse écrite et à l'argumentation et la prise de position discursive ménagent une place importante à la problématique de la neutralisation de la subjectivité et de la désinscription énonciative. Le présent numéro reprend, prolonge et spécifie certaines des interrogations et propositions émergeant de ces numéros et de différents travaux du champ des sciences du langage. Il se propose de mener la réflexion sur la « texture énonciative » (Moirand 2000) du discours de presse à travers ses différentes réalisations sémiotiques et institutionnelles et sur la responsabilité des instances productrices de ce discours (Charaudeau 1997, Rabatel 2005) . Le cadre théorique de référence est celui d'une linguistique des discours et des textes qui reconnaît comme essentielle le rôle des formations socio-discursives et s'attache à explorer les dimensions dialogiques foncières du discours (Adam, Maingueneau, etc.). La subjectivité généralisée est envisagée comme inhérente à l'activité langagière ; elle ne saurait s'appréhender exclusivement à travers le filtre de ses marques formelles et génériques déclarées mais s'actualise à travers une palette de « simulacres énonciatifs ». Les mutations sémio-technologiques qui affectent l'écriture médiatique − presse en ligne et ses incidences, développement du multimédia, multiplication des hyperstructures (Lugrin 2000 : 69) −, la variété de ses organes et de ses manifestations, les interrelations entre ses formes et ses genres en perpétuelle négociation et redéfinition interviennent de manière décisive dans l'organisation de l'interdiscursivité et les modalités de la prise en charge énonciative. Le numéro analyse un certain nombre de phénomènes d'effacement énonciatif (Vion 2001, Rabatel 2004) dans la presse et réinterroge à cette aune les problématiques de l'argumentation, de la rhétorique, de la construction de la référence, etc

Formation of methyl radicals derived from cumene hydroperoxide in reconstructed human epidermis: an EPR spin trapping confirmation by using 13 C-substitution

International audienc

Disentangling magnetic hardening and molecular spin chain contributions to exchange bias in ferromagnet/molecule bilayers

We performed SQUID and FMR magnetometry experiments to clarify the relationship between two reported magnetic exchange effects arising from interfacial spin-polarized charge transfer within ferromagnetic metal (FM)/molecule bilayers: the magnetic hardening effect, and spinterface-stabilized molecular spin chains. To disentangle these effects, both of which can affect the FM magnetization reversal, we tuned the metal phthalocyanine molecule central site's magnetic moment to selectively enhance or suppress the formation of spin chains within the molecular film. We find that both effects are distinct, and additive. In the process, we 1) extended the list of FM/molecule candidate pairs that are known to generate magnetic exchange effects, 2) experimentally confirmed the predicted increase in anisotropy upon molecular adsorption; and 3) showed that spin chains within the molecular film can enhance magnetic exchange. This magnetic ordering within the organic layer implies a structural ordering. Thus, by distengangling the magnetic hardening and exchange bias contributions, our results confirm, as an echo to progress regarding inorganic spintronic tunnelling, that the milestone of spintronic tunnelling across structurally ordered organic barriers has been reached through previous magnetotransport experiments. This paves the way for solid-state devices studies that exploit the quantum physical properties of spin chains, notably through external stimuli.Comment: Non

Encoding information onto the charge and spin state of a paramagnetic atom using MgO tunnelling spintronics

An electrical current that flows across individual atoms or molecules can generate exotic quantum-based behavior, from memristive effects to Coulomb blockade and the promotion of quantum excited states. These fundamental effects typically appear one at a time in model junctions built using atomic tip or lateral techniques. So far, however, a viable industrial pathway for such discrete state devices has been lacking. Here, we demonstrate that a commercialized device platform can serve as this industrial pathway for quantum technologies. We have studied magnetic tunnel junctions with a MgO barrier containing C atoms. The paramagnetic localized electrons due to individual C atoms generate parallel nanotransport paths across the micronic device as deduced from magnetotransport experiments. Coulomb blockade effects linked to tunnelling magnetoresistance peaks can be electrically controlled, leading to a persistent memory effect. Our results position MgO tunneling spintronics as a promising platform to industrially implement quantum technologies

Biochemical alterations in duckweed and algae induced by carrier solvents: Selection of an appropriate solvent in toxicity testing

Carrier solvents are often used in aquatic toxicity testing for test chemicals with hydrophobic properties. However, the knowledge of solvent effects on test organisms remains limited. This study aimed to understand biochemical effects of the four common solvents ((methanol, ethanol, acetone and dimethylsulfoxide (DMSO)) on two test species Lemna minor and Raphidocelis subcapitata by applying Fourier transform infrared spectroscopy (FTIR) coupled with multivariate analysis in order to select appropriate solvents in the toxicity testing. The results showed biochemical variations associated with solvent treatments at different doses on test species. From the obtained infrared spectra, the structures of lipid membrane and protein phosphorylation in the test species were found sensitive to the solvents. Methanol and ethanol mainly affected the protein secondary structure, while acetone and DMSO primarily induced the alterations of carbohydrate and proteins in the test species. The FTIR results demonstrated that methanol and ethanol showed higher biochemical alterations in the test species than acetone and DMSO, especially at the high doses (0.1% and 1% v/v). Based on the growth inhibition and FTIR spectroscopy, acetone and DMSO can be used as carrier solvent in the toxicity testing when their doses are lower than 0.1% v/v. This article is protected by copyright. All rights reserve

Revisiting the pro-oxidant activity of copper: interplay of ascorbate, cysteine and glutathione

Copper (Cu) is essential for most organisms, but it can be poisonous in excess, through mechanisms such as protein aggregation, trans-metallation and oxidative stress. Latter could implicate the formation of potentially harmful Reactive Oxygen Species (ROS: O2•–, H2O2 and HO•) via the redox cycling between Cu(II)/Cu(I) states in the presence of dioxygen and physiological reducing agents such as ascorbate (AscH), cysteine (Cys) and the tripeptide glutathione (GSH). Although the reactivity of Cu with these reductants has been previously investigated, the reactions taking place in a more physiologically-relevant mixture of these biomolecules are not known. Hence, we report here on the reactivity of Cu with binary and ternary mixtures of AscH, Cys and GSH. By measuring ascorbate and thiol oxidation, as well as HO• formation, we show that Cu reacts preferentially with GSH and Cys, halting AscH oxidation and also HO• release. This could be explained by the formation of Cu-thiolate clusters with both GSH and, as we first demonstrate here, Cys. Moreover, we observed a remarkable acceleration of Cu-catalysed GSH oxidation in the presence of Cys. We provide evidence that both thiol-disulfide exchange and the generated H2O2 contribute to this effect. Based on these findings, we speculate that Cu-induced oxidative stress may be mainly driven by GSH depletion and/or protein disulfide formation rather than by HO• and envision a synergistic effect of Cys on Cu toxicity

Potential of EPR spin-trapping to investigate in situ free radicals generation from skin allergens in reconstructed human epidermis: cumene hydroperoxide as proof of concept

The first step in the development of skin sensitisation to a chemical, and in the elicitation offurther allergic contact dermatitis (ACD), is the binding of the allergen to skin proteins after pene-trating into the epidermis. The so-formed antigenic adduct is then recognised by the immunesystem as foreign to the body. Sensitising organic hydroperoxides derived from autoxidation ofnatural terpenes are believed to form antigens through radical-mediated mechanisms, althoughthis has not yet been established. So far,in vitroinvestigations on reactive radical intermediatesderived from these skin sensitisers have been conducted in solution, yet with experimental condi-tions being far away from real-life sensitisation. Herein, we report for the first time, the potentialuse of EPR spin-trapping to study thein situgeneration of free radicals derived from cumenehydroperoxide CumOOH in a 3D reconstructed human epidermis (RHE) model, thus much closerto what may happenin vivo. Among the undesirable effects associated with dermal exposure toCumOOH, it is described to cause allergic and irritant dermatitis, being reported as a significantsensitiser. We considered exploiting the usage of spin-trap DEPMPO as an extensive view of allsort of radicals derived from CumOOH were observed all at once in solution. We showed that inthe EpiskinTMRHE model, both by incubating in the assay medium and by topical application,carbon radicals are mainly formed by redox reactions suggesting the key role of CumOOH-derived carbon radicals in the antigen formation process

Nucleic Acids Res

Site-directed spin labeling is emerging as an essential tool to investigate the structural and dynamical features of RNA. We propose here an enzymatic method, which allows the insertion of a paramagnetic center at a specific position in an RNA molecule. The technique is based on a segmental approach using a ligation protocol with T4 RNA ligase 2. One transcribed acceptor RNA is ligated to a donor RNA in which a thio-modified nucleotide is introduced at its 5'-end by in vitro transcription with T7 RNA polymerase. The paramagnetic thiol-specific reagent is subsequently attached to the RNA ligation product. This novel strategy is demonstrated by introducing a paramagnetic probe into the 55 nucleotides long RNA corresponding to K-turn and Specifier Loop domains from the Bacillus subtilis tyrS T-Box leader RNA. The efficiency of the coupling reaction and the quality of the resulting spin-labeled RNA were assessed by Mass Spectrometry, Electron Paramagnetic Resonance (EPR) and Nuclear Magnetic Resonance (NMR). This method enables various combinations of isotopic segmental labeling and spin labeling schemes, a strategy that will be of particular interest to investigate the structural and dynamical properties of large RNA complexes by NMR and EPR spectroscopies