Differential Antagonism of Human Innate Immune Responses by Tick-Borne Phlebovirus Nonstructural Proteins

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ANTI-INFLAMMATORY, ANALGESIC AND GC – MS ANALYSIS OF ESSENTIAL OIL OF ALPINIA CALCARATA RHIZOME

In the present study, essential oil isolated from Alpinia calcarata was analyzed and also assessed for acute toxicity, anti-inflammatory and analgesic activities in animals. The essential oil isolated from Alpinia calcarata was analyzed by using GC-MS on a combined GC-MS instrument. For evaluation of the antiinflammatory property “carrageenan induced paw edema model ” served as acute model. “Acetic acid induced writhing response model ” was used to assess analgesic activity in mice. The major components of essential oils isolated from Alpinia calcarata were Camphene (3.86%), Betamyrcene(4.39%), Eucalyptol(14.05%), Linalol(2.48%), Pyrazine(1.72%), L-camphor(7.90%) and Berneol(5.67%). Intraperitoneal injection of essential oil isolated from Alpinia calcarata significantly (P<0.05) suppressed the paw edema induced by carrageenan in two different dose levels studies namely 400mg/kg and 380mg/kg. Intraperitoneal injection of essential oil also significantly attenuated the acetic acid induced writhing response in three different dose levels studies namely 200 mg/kg (significant at P<0.05), 400mg/kg (significant at P<0.01) and 600mg/kg (significant at P<0.01). These studies suggest that essential oil isolated from Alpinia calcarata might possess significant anti-inflammatory activity and analgesic effect and could be a potential source for treatment of different inflammatory diseases