Limitaciones en el levantamiento digital de bienes patrimoniales con tipología de torre

[EN] Digital terrestrial survey techniques such as photogrammetry (SfM) and 3D laser scanning have certain limitations. These depend on the tools used, geometry, lighting conditions and distance from the element which is being surveyed. Therefore, the aim of this study is to describe a method for the correct digital survey of high elements (with tower typology) when working with such restrictions, as well as to verify the relevance of its usefulness. This methodology is applied to two bell towers located in Ravenna (Italy) allowing a series of tests and reasoning on geometric accuracy and quality of the texture obtained in final models.[ES] Las técnicas de levantamiento digital terrestres como la fotogrametría (SfM) y el láser escáner 3D (TLS) poseen ciertas limitaciones en función de las herramientas utilizadas y de la geometría, condiciones de iluminación y distancia del elemento con el que se trabaja. Por ello, el objetivo de este estudio es describir un método para el correcto levantamiento digital de elementos altos (con tipología de torre) cuando se trabaja con dichas restricciones, así como verificar la pertinencia de su utilidad. Dicha metodología se aplica a dos torres campanario situadas en Ravenna (Italia) permitiendo realizar una serie de ensayos y razonamientos sobre la precisión y la calidad de la textura obtenida en los modelos finales.Molina Sánchez, S.; Juan Vidal, F.; Cipriani, L.; Denia Ríos, JL. (2021). Limitations in the digital survey of heritage assets with tower typology. EGA Expresión Gráfica Arquitectónica. 26(42):76-89. https://doi.org/10.4995/ega.2021.14749OJS7689264

Diseño de un Equipo Didáctico de Manufactura CNC

El proyecto consiste en un Equipo Didáctico de Manufactura CNC para realizar prácticas a nivel licenciatura y maestría en las carreras de mecatrónica, mecánica, electromecánica e industrial. El diseño consiste en una máquina que consta de una mesa con ranuras, una prensa, y tres ejes X, Y y Z. Los ejes serán controlados por un sistema CNC mediante un softwar

RT-PCR multiplex para la detección simultánea de las mutaciones FLT3-ITD/NPM-1/AML1-ETO asociadas a Leucemia Mieloide Aguda

La Leucemia Mieloide Aguda (LMA) representa un grupo de neoplasias muy heterogéneo. Las aberraciones citogenéticas detectadas en el momento del diagnóstico son el marcador pronóstico más comúnmente utilizado. Sin embargo, el 20% de los casos de LMA presentan un cariotipo normal. Dentro de este grupo de pacientes la presencia de mutaciones del tipo FLT3-ITD se considera de mal pronóstico. Sin embargo, la presencia de la mutación NMP1 o AML1-ETO se asocia a un mejor pronóstico. En este contexto, el objetivo de este trabajo es el desarrollo de una técnica de diagnóstico molecular hematológico, que permita la detección simultánea de mutaciones para estos tres genes. Hemos desarrollado un método basado en la reacción en cadena de la polimerasa (PCR) que permite amplificar y visualizar simultáneamente estos 3 marcadores tanto desde ARN (un paso) como desde ADNc (dos pasos). De las 28 muestras analizadas, 6 (21,42 %) muestras fueron positivas para FLT3-ITD, 7 para NPM-1 (25%) y otras 4 (14,28) para AML1-ETO. Al comparar ambos métodos (ADNc vs ARN) con métodos convencionales los resultados de las 28 muestras estudiadas fue equivalente en el 100% de los casos, demostrando la robustez de los mismos.Acute Myeloid Leukemia (AML) is a heterogeneous group of neoplasms. The cytogenetic aberrations detected at the time of diagnosis are most commonly used as prognostic marker. However, 20% of AML patients exhibit a normal karyotype. Within this group of patients the presence of FLT3 -ITD mutations type is considered of poor prognosis. However, the presence of AML1 –ETO or NMP-1 or mutation is associated with a better prognosis. In this context, the aim of this work is to develop a technique of molecular diagnostic in hematology, allowing the simultaneous detection of mutations for these three genes. We have developed a method based on PCR that simultaneously amplifies and visualizes these three molecular markers both from RNA (one- step) and from cDNA (step two). Of the 28 samples tested, 6 (21.42%) samples were positive for FLT3 -ITD, 7 for NPM- 1 (25% ) and 4 ( 14,28 ) for AML1 -ETO . When comparing the two methods (cDNA vs RNA) by conventional techniques the obtained results from the 28 samples tested was equivalent in 100% of cases, demonstrating the robustness of this development

Construction of a novel Pichia pastoris strain for production of xanthophylls

In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-strains harboring astaxanthin-encoding genes from carotenogenic microorganism, and thus capable of producing xanthophyllic compounds. We designed and constructed a plasmid (pGAPZA-WZ) containing both the β-carotene ketolase (crtW) and β-carotene hydroxylase (crtZ) genes from Agrobacterium aurantiacum, under the control of the GAP promoter and containing an AOX-1 terminator. The plasmid was then integrated into the P. pastoris Pp-EBIL strain genomic DNA, producing clone Pp-EBILWZ. The recombinant P. pastoris (Pp-EBILWZ) cells exhibited a strong reddish carotenoid coloration and were confirmed, by HPLC, to produce not only the previous described carotenoids lycopene and β-carotene, but also de novo synthesized astaxanthin.J. M. A-G. is the recipient of an AECID scholarship from the Spanish Foreign Affairs MinistryS

Study of the application of PCM to thermal insulation of UUV hulls using Network Simulation Method

The Unmanned Underwater Vehicles (UUV) designed for the detection and neutralization of naval mines are characterized by their high requirements regarding the reduction of the signatures of a vessel (thermal, acoustic, magnetic, etc.), all of them must be reduced in order not to be detected by the mines during the mission. This research work focuses on the reduction of the thermal signature transmitted by the UUV hull, where the use of phase change materials (PCM) is proposed as thermal insulation, placing it between the internal heat source of the UUV and the pressure hull, with the aim of drastically increasing the time in which the external face of the hull of the UUV is within a range of 3 °C and 5 °C temperature increase, allowing it to perform its mission without being detected by the thermal sensors of current naval mines. The simulation will be carried out using the Network Simulation Method for the resolution of the governing equations, which will allow the optimization of the necessary insulation thickness according to the required mission profile. In addition, it is proposed an expression that relates the PCM thickness and the time needed to reach a certain temperature increase

A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype

Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work represents the first time that an inducible flocculation phenotype has been generated in a non flocculent strain of Kluyveromyces marxianus. This was accomplished by expressing Saccharomyces cerevisiae FLO5 gene in K. marxianus CECT 11769 strain. The FLO 5 gene was placed under the control of an EPG promoter, not repressed by glucose and induced by anoxia. Our experimental approach successfully generated two novel K. marxianus flocculent phenotypes: one inducible and one constitutive. The constitutive phenotype originated from deletions in the FLO5 promoter region, indicating the existence of putative upstream repressor site involved in oxygen regulation of the EPG1 promoter. The novel strains here generated had a unique set of characteristics that provided an advantage, over the wild-type strain, for the industrial co-production of ethanol and polygalacturonase.This work was supported by the AECID (Grant no. A/024951/09 and A/ 030029/10)S

Automatic Segmentation of the Retinal Nerve Fiber Layer by Means of Mathematical Morphology and Deformable Models in 2D Optical Coherence Tomography Imaging

[EN] Glaucoma is a neurodegenerative disease process that leads to progressive damage of the optic nerve to produce visual impairment and blindness. Spectral-domain OCT technology enables peripapillary circular scans of the retina and the measurement of the thickness of the retinal nerve fiber layer (RNFL) for the assessment of the disease status or progression in glaucoma patients. This paper describes a new approach to segment and measure the retinal nerve fiber layer in peripapillary OCT images. The proposed method consists of two stages. In the first one, morphological operators robustly detect the coarse location of the layer boundaries, despite the speckle noise and diverse artifacts in the OCT image. In the second stage, deformable models are initialized with the results of the previous stage to perform a fine segmentation of the boundaries, providing an accurate measurement of the entire RNFL. The results of the RNFL segmentation were qualitatively assessed by ophthalmologists, and the measurements of the thickness of the RNFL were quantitatively compared with those provided by the OCT inbuilt software as well as the state-of-the-art methods.This work was partially funded by Spanish National projects AES2017-PI17/00771 and AES2017-PI17/00821 (Instituto de Salud Carlos III), PID2019-105142RB-C21 (AI4SKIN) (Spanish Ministry of Economy and Competitiveness), PTA2017-14610-I (State Research Spanish Agency), regional project 20901/PI/18 (Fundacion Seneca) and Polytechnic University of Valencia (PAID-01-20).Berenguer-Vidal, R.; Verdú-Monedero, R.; Morales-Sánchez, J.; Sellés-Navarro, I.; Del Amor, R.; García-Pardo, JG.; Naranjo Ornedo, V. (2021). Automatic Segmentation of the Retinal Nerve Fiber Layer by Means of Mathematical Morphology and Deformable Models in 2D Optical Coherence Tomography Imaging. Sensors. 21(23):1-30. https://doi.org/10.3390/s21238027S130212

Formulación frecuencial de la ecuación de Euler-Lagrange para modelos deformables

Deformable models, i.e., active contours and active meshes, are useful tools in image processing and computer vision, because of their ability to take the shape of real 2D/3D objects. The original formulation of the models is defined on the spatial domain and it is based on the minimization of an energy functional through the Euler-Lagrange differential equations. In this paper, a new formulation is proposed in the frequency domain, for the adaptation process of the contours and meshes to the external forces. The presented frequency approach provides an easier and more computationally efficient implementation, allowing the use of deformable models to a greater number of areas.Este trabajo está financiado por el Ministerio de Ciencia y Tecnología, bajo el proyecto TEC2006-13338/TC

¿Mejoran los resultados con los nuevos instrumentos de evaluación de las asignaturas?

La puesta en marcha de los planes de estudios del plan Bolonia ha supuesto un cambio en los criterios de evaluación de las asignaturas de Contabilidad Financiera, impartidas en el primer curso del grado en Administración y Dirección de Empresas (ADE) de la Universidad de Murcia. El objeto de este trabajo consiste en determinar si la introducción de estos nuevos criterios ha supuesto un factor de cambio: a) en la calificación final de la asignatura, y b) en los conocimientos adquiridos (medidos a través de la prueba teórico-práctica final). Para analizar el primer objetivo comparamos, por un lado, los resultados obtenidos por los alumnos de las asignaturas Contabilidad Financiera I y II del grado en ADE durante los cursos académicos 2009/2010 y 2010/2011 con los obtenidos en las asignaturas de Contabilidad Financiera de la Licenciatura en ADE y la Diplomatura en Ciencias Empresariales en los dos cursos académicos inmediatamente anteriores: 2007/2008 y 2008/2009. Y para analizar el segundo objetivo comparamos la nota final (valorada sobre 10) de la prueba teórico-práctica de las asignaturas Contabilidad Financiera I y Contabilidad Financiera II en los cursos 2009/2010 y 2010/2011, con la nota final (valorada sobre 10) de dichas asignaturas en los cursos 2007/2008 y 2008/2009, con el fin de estudiar si los criterios adicionales de valoración influyen en la nota final a igualdad de conocimientos respecto a cursos anteriores. Los resultados evidencian una mejora de las calificaciones de los alumnos con los nuevos criterios de evaluación.Campus Mare Nostrum, Universidad Politécnica de Cartagena, Universidad de Murcia, Región de Murci

Rede iberoamericana de programas pós-consumo de medicamentos: passado, presente e futuro

Contamination by pharmaceuticals is a worldwide concern. Several studies have shown the deleterious effect that these products cause to ecosystems, including human beings. Among the strategies proposed to mitigate this problem are post- consumer drug management programs, mostly based on the concepts of Corporate Social Responsibility, Circular Economy, and Reverse Logistics. Four of these programs stand out, which have been successfully installed in Colombia (Punto Azul), Spain (SIGRE), Mexico (SINGREM) and Portugal (VALORMED). Together they conform the Ibero-American Network of Post-Consumer Drug Programs. This paper provides an overview of the origins of these programs, their current situation, and future projections.La contaminación por residuos farmacéuticos es una preocupación a nivel mundial. Diversos estudios han demostrado el efecto deletéreo que estos productos ocasionan sobre los ecosistemas en general, incluyendo los seres humanos. Entre las estrategias propuestas para mitigar esta problemática figuran los programas de gestión posconsumo de medicamentos, basados, en su mayoría, en conceptos de Responsabilidad Social Empresaria, Economía Circular y Logística Inversa. De tales programas se destacan cuatro, que han sido instalados con éxito, en Colombia (Punto Azul), España (SIGRE), México (SINGREM) y Portugal (VALORMED). En forma conjunta integran la Red Iberoamericana de Programas Posconsumo de Medicamentos. En este trabajo se brinda un panorama de los orígenes de estos programas, su situación actual y las proyecciones futuras.A contaminação por resíduos farmacêuticos é uma preocupação mundial. Vários estudos têm mostrado o efeito deletério que esses produtos causam nos ecossistemas em geral, inclusive no ser humano. Entre as estratégias propostas para mitigar esse problema estão os programas de gestão de medicamentos pós-consumo, em sua maioria baseados nos conceitos de Responsabilidade Social Corporativa, Economia Circular e Logística Reversa. Destacam-se quatro desses programas, implantados com sucesso na Colômbia (Punto Azul), Espanha (SIGRE), México (SINGREM) e Portugal (VALORMED). Juntos, eles compõem a Rede Ibero-Americana de Programas Pós-Consumo de Medicamentos. Este artigo fornece uma visão geral sobre as origens desses programas, sua situação atual e projeções futuras