USSR Space Life Sciences Digest, issue 21

This is the twenty-first issue of NASA's USSR Space Life Sciences Digest. It contains abstracts of 37 papers published in Russian language periodicals or books or presented at conferences and of a Soviet monograph on animal ontogeny in weightlessness. Selected abstracts are illustrated with figures and tables from the original. A book review of a work on adaptation to stress is also included. The abstracts in this issue have been identified as relevant to 25 areas of space biology and medicine. These areas are: adaptation, biological rhythms, body fluids, botany, cardiovascular and respiratory systems, cytology, developmental biology, endocrinology, enzymology, equipment and instrumentation, exobiology, gravitational biology, habitability and environmental effects, hematology, human performance, life support systems, mathematical modeling, metabolism, microbiology, musculoskeletal system, neurophysiology, operational medicine, perception, psychology, and reproductive system

Implementing a Digital Imaging Platform Using Tissue Analytics in a Level 1 Trauma Center

Background: More than 2.5 million people develop pressure ulcers (PUs) annually. PUs cost the U.S. $9.1-11.6 billion per year and add about$43,180 to a hospital stay. The Centers for Medicare Medicaid Services (CMS) stopped reimbursement for hospital-acquired pressure ulcers (HAPUs). Documentation of PUs must indicate if they are present on admission (POA) and include accurate stages and treatment plan of PUs. Aims/Objectives: To use Digital Imaging platform using Tissue Analytics (TA) along with the standard care protocol. The objectives of this study were to (1) Improve identification of PUs, (2) Increase accuracy of PU staging and (3) Improve documentation of PUs. Methods: This quality improvement study included a convenience sample of 55 adults with HAPUs. NDNQI measures and data from chart audits were collected. Demographic information, POAs and HAPUs, and Braden scores were analyzed along with a staff attitude’s survey. Results: 5.11 POAs per 1000 patient admissions (95% CI = [3.33, 7.84]) pre-interventions, and 2.25 POAs per 1000 patient admissions (95% CI = [1.17, 4.33]) post-intervention. POA prevalence rate remained about the same. Documentation of HAPU stages (χ2 (5) = 9.823, p = 0.059) was not statistically significant but documentation of POA staging was significant (χ2 (6) = 16.395, p = 0.003). The number of DTIs increased while Unstageable PUs decreased post-intervention. Braden score= 14.52 (SD = 3.65) pre-intervention and 14.56 (SD = 2.55) post-intervention. Staff Attitudes’ survey scores reflected poor PU prevention attitude (33.36). Conclusions: Digital imaging Platform using TA along with the standard protocol did not significantly improve the identification of POAs nor rate of documentation. Documented difference in staging was noted. Also, improved quality of documentation was noticed

USSR Space Life Sciences Digest, Issue 18

This is the 18th issue of NASA's USSR Life Sciences Digest. It contains abstracts of 50 papers published in Russian language periodicals or presented at conferences and of 8 new Soviet monographs. Selected abstracts are illustrated with figures and tables from the original. A review of a recent Aviation Medicine Handbook is also included. The abstracts in this issue have been identified as relevant to 37 areas of space biology and medicine. These areas are: adaptation, aviation medicine, biological rhythms, biospherics, body fluids, cardiovascular and respiratory systems, cytology, developmental biology, endocrinology, enzymology, equipment and instrumentation, exobiology, gastrointestinal system, genetics, gravitational biology, group dynamics, habitability and environmental effects, hematology, human performance, immunology, life support systems, man-machine systems, mathematical modeling, metabolism, microbiology, musculoskeletal system, neurophysiology, nutrition, operational medicine, perception, personnel selection, psychology, radiobiology, reproductive biology, space biology and medicine, and space industrialization

Convergent evolution of pregnancy-specific glycoproteins in human and horse

Pregnancy-specific glycoproteins (PSGs) are members of the carcinoembryonic antigen cell adhesion molecule (CEACAM) family that are secreted by trophoblast cells. PSGs may modulate immune, angiogenic and platelet responses during pregnancy. Until now, PSGs are only found in species that have a highly invasive (hemochorial) placentation including humans, mice and rats. Surprisingly, analyzing the CEACAM gene family of the horse, which has a non-invasive epitheliochorial placenta, with the exception of the transient endometrial cups, we identified equine CEACAM family members that seem to be related to PSGs of rodents and primates. We identified seven genes that encode secreted PSG-like CEACAMs. Phylogenetic analyses indicate that they evolved independently from an equine CEACAM1-like ancestor rather than from a common PSG-like ancestor with rodents and primates. Significantly, expression of PSG-like genes (CEACAM44, CEACAM48, CEACAM49 and CEACAM55) was found in non-invasive as well as invasive trophoblast cells such as purified chorionic girdle cells and endometrial cup cells. Chorionic girdle cells are highly invasive trophoblast cells that invade the endometrium of the mare where they form endometrial cups and are in close contact with maternal immune cells. Therefore, the microenvironment of invasive equine trophoblast cells has striking similarities to the microenvironment of trophoblast cells in hemochorial placentas, suggesting that equine PSG-like CEACAMs and rodent and primate PSGs have undergone convergent evolution. This is supported by our finding that equine PSG-like CEACAM49 exhibits similar activity to certain rodent and human PSGs in a functional assay of platelet–fibrinogen binding. Our results have implications for understanding the evolution of PSGs and their functions in maternal–fetal interactions

Assessment of the impact of the COVID-19 pandemic on health services use

OBJECTIVES: The coronavirus disease of 2019 (COVID-19) pandemic declared by the World Health Organization on March 11, 2020 impacted healthcare services with provider and patient cancellations, delays, and patient avoidance or delay of emergency department or urgent care. Limited data exist on the population proportion affected by delayed healthcare, which is important for future healthcare planning efforts. Our objective was to evaluate the impact of the COVID-19 pandemic on healthcare service cancellations or delays and delays/avoidance of emergency/urgent care overall and by population characteristics. STUDY DESIGN: This was a cross-sectional study. METHODS: Our sample (n = 2314) was assembled through a phone survey from 8/12/2020-10/27/2020 among non-institutionalized St. Louis County, Missouri, USA residents ≥18 years. We asked about provider and patient-initiated cancellations or delays of appointments and pandemic-associated delays/avoidance of emergency/urgent care overall and by participant characteristics. We calculated weighted prevalence estimates by select resident characteristics. RESULTS: Healthcare services cancellations or delays affected ∼54% (95% CI 50.6%-57.1%) of residents with dental (31.1%, 95% CI 28.1%-34.0%) and primary care (22.1%, 95% CI 19.5%-24.6%) being most common. The highest prevalences were among those who were White, ≥65 years old, female, in fair/poor health, who had health insurance, and who had ≥1 medical condition. Delayed or avoided emergency/urgent care impacted ∼23% (95% CI 19.9%-25.4%) of residents with a higher prevalence in females than males. CONCLUSIONS: Healthcare use disruptions impacted a substantial proportion of residents. Future healthcare planning efforts should consider these data to minimize potential morbidity and mortality from delayed care

Respiratory and allergic health effects in a young population in proximity of a major industrial park in Oman

Background: The Sohar Industrial Zone (SIZ), Oman, which started operating in 2006, contains many industries that potentially affect health of the local population. The aim of this study was to evaluate the health effects in a young population living near SIZ. Methods: Retrospective health care visits for acute respiratory diseases (ARD), asthma, conjunctivitis and dermatitis for children of ages 5-10, ≥20 km to represent high, intermediate and control exposure zones respectively. Age and gender-specific monthly counts of visits of the selected diseases were modeled using generalized additive models controlling for time trends. The high and intermediate exposure zones were later combined together due to similarity of associations. Exposure effect modification by age, gender and socioeconomic status (SES) was also tested. Results: Living within 10 km from SIZ showed greater association with ARD (RR: 2.5; 95% CI: 2.3-2.7)), asthma (RR: 3.7; 95% CI: 3.1-4.5), conjunctivitis (RR: 3.1; 95% CI: 2.9-3.5), and dermatitis (RR: 2.7; 95% CI: 2.5-3.0) when compared to the control zone. No differences in associations were found for gender and SES groups; greater effects were noticed in the ≤14 years old group for asthma. Conclusion: This is the first study conducted in Oman examining the health effects of a young population living in proximity of an industrial park. We hope that these findings will contribute in future developments of environmental and public health policies in Oman

Lamina-specific population encoding of cutaneous signals in the spinal dorsal horn using multi-electrode arrays

The dorsal spinal cord (DH) is a complex laminar structure integrating peripheral signals into the central nervous system. Spinal somatosensory processing is commonly measured electrophysiologically in vivo by recording the activity of individual Wide Dynamic Range neurons in the deep DH and extrapolating their behaviour to all cells in every lamina. This fails to account for the specialised processes that occur in each lamina and the considerable heterogeneity in cellular phenotype within and between laminae. Here we overcome this oversimplification by employing linear multi‐electrode arrays (MEAs) in the DH of anaesthetized rats to simultaneously measure activity across all laminae. The MEAs, comprised of 16‐channels, were inserted into the lumbar dorsal horn and peripheral neurones activated electrically via transcutaneous electrodes and ethologically with von Frey hairs (vFh) or an aluminium heating block. Ascending electrical stimuli showed fibre thresholds with distinct dorso‐ventral innervation profiles. Wind up was observed across the DH during the C‐fibre and post‐discharge latencies following 0.5Hz stimulation. Intrathecal application of morphine (5ng/50ul) significantly reduced Aδ and C‐fibre evoked activity in deep and superficial DH. Light vFhs (≤10g) predominantly activated intermediate and deep laminae whereas noxious vFh (26g) also activated the superficial laminae. Noxious heat (55°C) induced significantly greater activity in the superficial and deep laminae than the innocuous control (30°C). The application of these arrays produced the first description of the processing of innocuous and noxious stimuli throughout the intact DH

Investigating the Role of the Organic Cation in Formamidinium Lead Iodide Perovskite Using Ultrafast Spectroscopy

Organic cation rotation in hybrid organic–inorganic lead halide perovskites has previously been associated with low charge recombination rates and (anti)­ferroelectric domain formation. Two-dimensional infrared spectroscopy (2DIR) was used to directly measure 470 ± 50 fs and 2.8 ± 0.5 ps time constants associated with the reorientation of formamidinium cations (FA⁺, NH₂CHNH₂⁺) in formamidinium lead iodide perovskite thin films. Molecular dynamics simulations reveal the FA⁺ agitates about an equilibrium position, with NH₂ groups pointing at opposite faces of the inorganic lattice cube, and undergoes 90° flips on picosecond time scales. Time-resolved infrared measurements revealed a prominent vibrational transient feature arising from a vibrational Stark shift: photogenerated charge carriers increase the internal electric field of perovskite thin films, perturbing the FA⁺ antisymmetric stretching vibrational potential, resulting in an observed 5 cm^–1 shift. Our 2DIR results provide the first direct measurement of FA⁺ rotation inside thin perovskite films, and cast significant doubt on the presence of long-lived (anti)­ferroelectric domains, which the observed low charge recombination rates have been attributed to

SMAD1/5 signaling in the early equine placenta regulates trophoblast differentiation and chorionic gonadotropin secretion.

TGFβ superfamily proteins, acting via SMAD (Sma- and Mad-related protein)2/3 pathways, regulate placental function; however, the role of SMAD1/5/8 pathway in the placenta is unknown. This study investigated the functional role of bone morphogenetic protein (BMP)4 signaling through SMAD1/5 in terminal differentiation of primary chorionic gonadotropin (CG)-secreting trophoblast. Primary equine trophoblast cells or placental tissues were isolated from day 27-34 equine conceptuses. Detected by microarray, RT-PCR, and quantitative RT-PCR, equine chorionic girdle trophoblast showed increased gene expression of receptors that bind BMP4. BMP4 mRNA expression was 20- to 60-fold higher in placental tissues adjacent to the chorionic girdle compared with chorionic girdle itself, suggesting BMP4 acts primarily in a paracrine manner on the chorionic girdle. Stimulation of chorionic girdle-trophoblast cells with BMP4 resulted in a dose-dependent and developmental stage-dependent increase in total number and proportion of terminally differentiated binucleate cells. Furthermore, BMP4 treatment induced non-CG-secreting day 31 chorionic girdle trophoblast cells to secrete CG, confirming a specific functional response to BMP4 stimulation. Inhibition of SMAD2/3 signaling combined with BMP4 treatment further enhanced differentiation of trophoblast cells. Phospho-SMAD1/5, but not phospho-SMAD2, expression as determined by Western blotting was tightly regulated during chorionic girdle trophoblast differentiation in vivo, with peak expression of phospho-SMAD1/5 in vivo noted at day 31 corresponding to maximal differentiation response of trophoblast in vitro. Collectively, these experiments demonstrate the involvement of BMP4-dependent pathways in the regulation of equine trophoblast differentiation in vivo and primary trophoblast differentiation in vitro via activation of SMAD1/5 pathway, a previously unreported mechanism of TGFβ signaling in the mammalian placenta