Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS

Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. a-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.Authors are grateful to Dr Zélia dos Santos Azevedo, Faculty of Sciences, University of Porto, who loaned LC/DAD-ESI/MS and for all technical assistance. The authors also are grateful for the help of the Foundation for the Science and Technology (FCT, Portugal) for financial support and also thank FCT for doctoral grant SFRH/BD/74979/2010.info:eu-repo/semantics/publishedVersio

Is the deinstitutionalisation of alternative care a ‘wicked problem’? A qualitative study exploring the perceptions of child welfare practitioners and policy actors in Thailand

This study examined deinstitutionalisation in Thailand. Qualitative interviews were conducted with a total of 27 child welfare practitioners and policy actors to explore their perceptions of Thai alternative care provision. Findings show that participants perceive deinstitutionalisation as a complex policy challenge. Some felt that the institutions were necessary in order to meet demand, while others felt that cultural barriers prevent a shift to family-based approaches, such as foster care. However, data suggest that it would be difficult to characterise deinstitutionalisation as a ‘wicked policy problem’ as participants were hopeful for change, citing increased family- strengthening policies alongside efforts to implement foster care

The urgent need for microbiology literacy in society

Contains fulltext : 204933.pdf (publisher's version ) (Open Access

Anaerobic reactor applied to laundry wastewater treatment : unveiling the microbial community by gene and genome-centric approaches

Gene and genome-centric approaches were applied to investigate the microbial taxonomic and metabolic diversity profiles from a lab-scale anaerobic reactor applied to laundry wastewater treatment with increasing concentrations of linear alkylbenzene sulfonate (LAS). Therefore, metagenomic analysis was carried out with reactor samples collected under three conditions, (i) Stage I, the reactor was fed with synthetic medium, (ii) Stage II, synthetic medium was replaced by diluted laundry wastewater with specific LAS loading rates (SLLRs) of 1.0 ± 0.3 mgLASgTVS−1d−1 and (iii) Stage III, SLLRs increased to 2.7 ± 0.7 mgLASgTVS−1d−1. Shotgun metagenomic sequencing was performed on an Illumina HiSeq 2 × 150 bp platform. Gene-centric analysis of each step of the metabolic pathway (fumarate addition, β-oxidation, ring cleavage and desulfonation) for anaerobic aromatic degradation showed eight over-representative genera (Achromobacter, Pelodictyon, Pseudomonas, Psychrobacter, Rhodococcus, Stenotrophomonas, Sulfurovum and Syntrophobacter), suggesting a microbial core with an important role in LAS biodegradation. Some of these genera were also recovered through a differential binning method, representing fifteen bacterial and one archaeal metagenome-assembled genomes (MAGs). Biodegradation pathway reconstruction of LAS using six MAGs unveiled the syntrophism for complete degradation of the LAS molecule. Only the MAGs with taxonomic annotation for Syntrophobacter showed genetic potential for fumarate addition, whereas in the ring cleavage, there was a predominance of genes in MAGs with taxonomic annotation for Pseudomonas fragi and Rhodococcus. This work represents the first report of genome-centric approach to study biological reactors applied in anionic surfactant degradation, contributing with detailed metabolic information of the key microbial actors in LAS degradation and opening perspectives for future biotechnological strategies aiming at bioaugmentation and/or biostimulation of indigenous microbial populations149COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP0012014/16426–0; 2015/06246–

TSC1/2 mutations define a molecular subset of HCC with aggressive behaviour and treatment implication

© Published by the BMJ Publishing Group Limited. Objective We investigated the mutational landscape of mammalian target of rapamycin (mTOR) signalling cascade in hepatocellular carcinomas (HCCs) with chronic HBV background, aiming to evaluate and delineate mutation-dependent mechanism of mTOR hyperactivation in hepatocarcinogenesis. Design We performed next-generation sequencing on human HCC samples and cell line panel. Systematic mutational screening of mTOR pathway-related genes was undertaken and mutant genes were evaluated based on their recurrence. Protein expressions of tuberous sclerosis complex (TSC)1, TSC2 and pRPS6 were assessed by immunohistochemistry in human HCC samples. Rapamycin sensitivity was estimated by colony-formation assay in HCC cell lines and the treatment was further tested using our patient-derived tumour xenograft (PDTX) models. Results We identified and confirmed multiple mTOR components as recurrently mutated in HBV-associated HCCs. Of significance, we detected frequent (16.2%, n=18/111) mutations of TSC1 and TSC2 genes in the HCC samples. The spectrum of TSC1/2 mutations likely disrupts the endogenous gene functions in suppressing the downstream mTOR activity through different mechanisms and leads to more aggressive tumour behaviour. Mutational disruption of TSC1 and TSC2 was also observed in HCC cell lines and our PDTX models. TSC-mutant cells exhibited reduced colony-forming ability on rapamycin treatment. With the use of biologically relevant TSC2-mutant PDTXs, we demonstrated the therapeutic benefits of the hypersensitivity towards rapamycin treatment. Conclusions Taken together, our findings suggest the significance of previously undocumented mutation-dependent mTOR hyperactivation and frequent TSC1/2 mutations in HBV-associated HCCs. They define a molecular subset of HCC having genetic aberrations in mTOR signalling, with potential significance of effective specific drug therapy.© Published by the BMJ Publishing Group Limited. Objective We investigated the mutational landscape of mammalian target of rapamycin (mTOR) signalling cascade in hepatocellular carcinomas (HCCs) with chronic HBV background, aiming to evaluate and delineate mutation-dependent mechanism of mTOR hyperactivation in hepatocarcinogenesis. Design We performed next-generation sequencing on human HCC samples and cell line panel. Systematic mutational screening of mTOR pathway-related genes was undertaken and mutant genes were evaluated based on their recurrence. Protein expressions of tuberous sclerosis complex (TSC)1, TSC2 and pRPS6 were assessed by immunohistochemistry in human HCC samples. Rapamycin sensitivity was estimated by colony-formation assay in HCC cell lines and the treatment was further tested using our patient-derived tumour xenograft (PDTX) models. Results We identified and confirmed multiple mTOR components as recurrently mutated in HBV-associated HCCs. Of significance, we detected frequent (16.2%, n=18/111) mutations of TSC1 and TSC2 genes in the HCC samples. The spectrum of TSC1/2 mutations likely disrupts the endogenous gene functions in suppressing the downstream mTOR activity through different mechanisms and leads to more aggressive tumour behaviour. Mutational disruption of TSC1 and TSC2 was also observed in HCC cell lines and our PDTX models. TSC-mutant cells exhibited reduced colony-forming ability on rapamycin treatment. With the use of biologically relevant TSC2-mutant PDTXs, we demonstrated the therapeutic benefits of the hypersensitivity towards rapamycin treatment. Conclusions Taken together, our findings suggest the significance of previously undocumented mutation-dependent mTOR hyperactivation and frequent TSC1/2 mutations in HBV-associated HCCs. They define a molecular subset of HCC having genetic aberrations in mTOR signalling, with potential significance of effective specific drug therapy.Link_to_subscribed_fulltextLink_to_subscribed_fulltex

Diagnóstico da geração e da composição dos RCD de Fortaleza/CE Generation and composition diagnosis of C&D waste of Fortaleza/CE

Este trabalho objetivou apresentar os resultados de um diagnóstico anual da geração e da composição dos resíduos de construção e demolição de Fortaleza. Durante 12 meses, foram colhidas informações das empresas coletoras e da prefeitura, tais como a quantidade e o preço do metro cúbico do entulho coletado e o destino dado a ele. Foram ainda coletadas amostras de resíduos de construção e demolição nos locais de descarte licenciados pela prefeitura. Os resultados apontam que os locais licenciados recebem em média 702 toneladas.dia-1, sendo que a composição desses resíduos de construção e demolição é, em média, de 65% de restos de argamassa, concreto e material cerâmico. Constatou-se também que grande parte desse tipo de resíduo é descartado ilegalmente, sendo que os dois principais aterros irregulares somam uma área aproximada de 26 hectares. Estima-se que esse sistema movimenta R$4,5 milhões anualmente e que quase metade desse montante é aportado somente pela Prefeitura Municipal.<br>This article aimed to present the results of an annual diagnosis of generation and composition of Fortaleza’s construction and demolition waste. During 12 months CDW collection companies’ and the city hall’ information were researched, such as the amount and the price of the cubic meter of the collected wastes and the destiny given to them. Samples of construction and demolition waste were either collected at the licensed disposal places. The results point that licensed places receive 702 tons.day-1, on average, and the composition of this construction and demolition waste is made, on average, by 65$4.5 million annually and almost half of this amount is paid only by the Municipal City hall

NWChem: Past, Present, and Future

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