Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation

The identification of chromosomal rearrangements is of utmost importance for the diagnosis and classification of specific leukaemia subtypes and therefore has an impact on therapy choices in individual cases. The t(7;12)(q36;p13) is a cryptic rearrangement that is difficult to recognise using conventional cytogenetic methods and is often undetected by reverse transcription polymerase chain reaction due to the absence of a fusion transcript in many cases. Here we present a reliable and easy to use dual colour fluorescence in situ hybridisation assay for the detection of the t(7;12)(q36;p13) rearrangement. A comparison with previous similar work is given and advantages and limitations of this novel approach are discussed

Half-Life of 14^{14}O

We have measured the half-life of $^{14}$O, a superallowed $(0^{+} \to 0^{+})$ $\beta$ decay isotope. The $^{14}$O was produced by the $^{12}$C($^{3}$He,n)$^{14}$O reaction using a carbon aerogel target. A low-energy ion beam of $^{14}$O was mass separated and implanted in a thin beryllium foil. The beta particles were counted with plastic scintillator detectors. We find $t_{1/2} = 70.696\pm 0.052$ s. This result is $1.5\sigma$ higher than an average value from six earlier experiments, but agrees more closely with the most recent previous measurement.Comment: 10 pages, 5 figure

Normal values of blood pressure self-measurement in view of the 1999 World Health Organization-International Society of Hypertension guidelines

New guidelines for the management of hypertension have been published in 1999 by the World Health Organization (WHO) and the International Society of Hypertension (ISH). The WHO/ISH Committee has adopted in principle the definition and classification of hypertension provided by the JNC VI (1997). The new classification defines a blood pressure of 120/80 mm Hg as optimal and of 130/85 mm Hg as the limit between normal and high-normal blood pressure. It is unclear which self-measured home blood pressure values correspond to these office blood pressure limits. In this study we reevaluated data from our Dübendorf study to determine self-measured blood pressure values corresponding to optimal and normal office blood pressure using the percentiles of the (office and home) blood pressure distributions of 503 individuals (age, 20 to 90 years; mean age, 46.5 years; 265 men, 238 women). Self-measured blood pressure values corresponding to office values of 130/85 mm Hg and 120/80 mm Hg were 124.1/79.9 mm Hg and 114.3/75.1 mm Hg. Thus, we propose 125/80 mm Hg as a home blood pressure corresponding to an office blood pressure of 130/85 mm Hg (WHO 1999: normal) and 115/75 mm Hg corresponding to 120/80 mm Hg (optimal). Am J Hypertens 2000;13:940-943 © 2000 American Journal of Hypertension, Lt

Questions to Americans from Latin Americans

The following are some of the more stereotyped questions about the United States that are often in the minds of the people and are reflected in publica­tions in Latin America. They all have a thread of logic; some are Communist-inspired, some are not

Water exchange at a hydrated platinum electrode is rare and collective

We use molecular dynamics simulations to study the exchange kinetics of water molecules at a model metal electrode surface -- exchange between water molecules in the bulk liquid and water molecules bound to the metal. This process is a rare event, with a mean residence time of a bound water of about 40 ns for the model we consider. With analysis borrowed from the techniques of rare-event sampling, we show how this exchange or desorption is controlled by (1) reorganization of the hydrogen bond network within the adlayer of bound water molecules, and by (2) interfacial density fluctuations of the bulk liquid adjacent to the adlayer. We define collective coordinates that describe the desorption mechanism. Spatial and temporal correlations associated with a single event extend over nanometers and tens of picoseconds.Comment: 10 pages, 9 figure

Classifying faces by sex is more accurate with 3D shape information than with texture

Purpose: We compared quality of information available in 3D surface models versus texture maps for classifying human faces by sex. Methods: 3D surface models and texture maps from laser scans of 130 human heads (65 male, 65 female) were analyzed with separate principal components analyses (PCAs). Individual principal components (PCs) from the 3D head data characterized complex structural differences between male and female heads. Likewise, individual PCs in the texture analysis contrasted characteristically male vs. female texture patterns (e.g., presence/absence of facial hair shadowing). More formally, representing faces with only their projection coefficients onto the PCs, and varying the subspace from 1 to 50 dimensions, we trained a series of perceptrons to predict the sex of the faces using either the 3D or texture data. A "leave-one-out" technique was applied to measure the gen-eralizability of the perceptron's sex predictions. Results: While very good sex generalization performance was obtained for both representations, even with very low dimensional subspaces (e.g., 76.1 correct with only one 3D projection coefficient), the 3D data supported more accurate sex classification across nearly the entire range of subspaces tested. For texture, 93.8 correct sex generalization was achieved with a minimun subspace of 20 projection coefficients. For 3D data, 96.9 correct generalization was achieved with 17 projection coefficients. Conclusions: These data highlight the importance of considering the kinds of information available in different face representations with respect to the task demands

Ambiguity of gamma-ray tracking of "two-interaction" events

Tracking of gamma-ray interactions in germanium detectors can allow reconstruction of the photon paths, and is useful for many applications. Scrutiny of the kinematics and geometry of gamma rays which are Compton scattered only once prior to full absorption reveals that there are cases where even perfect spatial and energy resolution cannot resolve the true interaction sequence and consequently gamma-ray tracks cannot be reconstructed. The photon energy range where this ambiguity exists is from 255 keV to around 700 keV. This is a region of importance for nuclear structure research where two-point interactions are probable.Comment: 8 pages, 2 figure

Longitudinal Atomic Beam Spin Echo Experiments: A possible way to study Parity Violation in Hydrogen

We discuss the propagation of hydrogen atoms in static electric and magnetic fields in a longitudinal atomic beam spin echo (lABSE) apparatus. Depending on the choice of the external fields the atoms may acquire both dynamical and geometrical quantum mechanical phases. As an example of the former, we show first in-beam spin rotation measurements on atomic hydrogen, which are in excellent agreement with theory. Additional calculations of the behaviour of the metastable 2S states of hydrogen reveal that the geometrical phases may exhibit the signature of parity-(P-)violation. This invites for possible future lABSE experiments, focusing on P-violating geometrical phases in the lightest of all atoms.Comment: 6 pages, 4 figure

SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells

Cochlear hair cells use SK2 currents to shape responses to cholinergic efferent feedback from the brain. Using SK2-/- mice, we demonstrate that, in addition to their previously defined role in modulating hair cell membrane potentials, SK2 channels are necessary for long-term survival of olivocochlear fibers and synapses. Loss of the SK2 gene also results in loss of electrically driven olivocochlear effects in vivo, and down regulation of ryanodine receptors involved in calcium-induced calcium release, the main inducer of nAChR evoked SK2 activity. Generation of double-null mice lacking both the α10 nAChR gene, loss of which results in hypertrophied olivocochlear terminals, and the SK2 gene, recapitulates the SK2-/- synaptic phenotype and gene expression, and also leads to down regulation of α9 nAChR gene expression. The data suggest a hierarchy of activity necessary to maintain early olivocochlear synapses at their targets, with SK2 serving an epistatic, upstream, role to the nAChRs.Fil: Murthy, Vidya. Tufts University; Estados UnidosFil: Maison, Stéphane F.. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados UnidosFil: Taranda, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Tufts University; Estados UnidosFil: Haque, Nadeem. University of Notre Dame; Estados UnidosFil: Bond, Chris T.. Oregon Health Sciences University; Estados UnidosFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Adelman, John P.. Oregon Health Sciences University; Estados UnidosFil: Liberman, M. Charles. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados UnidosFil: Vetter, Douglas E.. Tufts University; Estados Unido