Homophobia, Fundamentalism, and Canadian Tolerance: Enabling Gay Games III in Vancouver

Gay Games III were held in Vancouver in August 1990. This paper analyzes the effects of three different moments of homophobic backlash faced by organizers of that event. While quiet institutional homophobia might have been tolerated, public representations of blatant homophobia could be mobilized by the organizers of Gay Games IIII to their advantage. The effects of a hateful campaign paradoxically functioned as an interesting condition of possibility for Gay Games' credibility. I then suggest that the limitations of these liberal strategies were shown up by the fractures within local lesbian and gay communities, demonstrating the weaknesses of accepting tolerance as a limit to the possible.Les troisièmes Jeux Gais ont eu lieu à Vancouver en août 1990. Ce document analyse les effets de trois moments différents du ressac homophobe auquel ont fait face les organisateurs de cet événement. Même si l’homophobie institutionnelle discrète aurait pu être tolérée, les organisateurs de ces jeux ont pu tourner à leur avantage les représentations publiques de l’homophobie flagrante. Les effets d’une campagne de haine ont paradoxalement été favorables à la possibilité de tenir des Jeux Gais crédibles. Je crois donc que les fractures observées dans les communautés lesbienne et gaie locales ont fait ressortir les limites de ces stratégies libérales en démontrant les failles de l’acceptation de la tolérance comme limite du possible

Estudo de viabilidade econômica de produção de glucoamilase por fermentação no estado sólido a partir de rejeito agroindustrial

No paradigma atual de crescentes preocupações ambientais, o aproveitamento de resíduos agroindustriais e a viabilização da produção de combustíveis de fontes renováveis são dois temas constantemente abordados. Neste trabalho, a utilização da torta de babaçu visa agregar valor, através de sua utilização como insumo para uma indústria de bioprocessos, a este resíduo que é gerado da extração do óleo de babaçu, utilizado para diversos fins nas indústrias de alimentos e química fina. A fermentação no estado sólido (FES) é uma escolha lógica para cumprir este tipo de objetivo, visto que potencialmente produz uma gama de produtos de interesse tecnológico a baixos custos e com poucos impactos ambientais. Neste contexto, a produção de enzimas por fungos filamentosos por FES se torna altamente atrativa, pois alia a adequação destes microrganismos às condições deste tipo de processo ao seu potencial reconhecido de produção de diversas enzimas extracelulares. Em especial, as enzimas glucoamilases possuem aplicação estratégica na produção de etanol a partir de matérias-primas amiláceas, sendo que o etanol é o principal combustível renovável da atualidade, tornando a diminuição dos custos de sua produção essencial. Em vista do exposto, o presente trabalho estudou a viabilidade econômica do processo de produção de glucoamilases por fermentação no estado sólido da torta de babaçu utilizando o Aspergillus awamori, microrganismo reconhecido na literatura como excelente produtor de glucoamilases. Para tanto, foram realizados experimentos para determinar parâmetros essenciais do processo e o software SuperPro Designer foi utilizado para realizar a síntese e a avaliação econômica do processo. A planta simulada foi capaz de produzir um preparado enzimático a um custo de aproximadamente US$5 por kg com capacidade de produção de 5 mil ton/ano, sendo que o preço de venda foi estimado em aproximadamente US$ 7 por kg. Este resultado mostra que o processo apresenta grande potencial e sugere que estudos posteriores devem ser realizados visando seu desenvolvimento em um processo industrial

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Síntese e caracterização de nanocompósitos de caulinita e TiO2 utilizando o método dos precursores poliméricos

No presente trabalho, utilizou-se a caulinita como material base para a obtenção de materiais nanocompósitos. A caulinita foi utilizada na forma in natura, tratada termicamente à 700 ºC e quimicamente com ácidos fosfórico e sulfúrico na concentração de 6 mol L-1. O objetivo dos tratamentos foi modificar e preparar a estrutura da caulinita para ser usada como um suporte para o TiO2. Por meio do método dos precursores poliméricos foi sintetizado o TiO2 e nanocompósitos à base de caulinita e TiO2. Os nanomateriais foram caracterizados por Difratometria de Raios X (DRX), UV-Vis, para determinar o Bandgap, Espectroscopia na Região do Infravermelho com Transformada de Fourier (FTIR), Microscopia Eletrônica de Varredura (MEV) e Espectroscopia de Energia Dispersiva (EDS). Por meio dos espectros de FTIR foi possível verificar as bandas características da caulinita in natura e o aparecimento de vibrações características da metacaulinita. A análise de DRX revelou que foi possível promover o aumento da cristalinidade das amostras de caulinita após tratamento com o citrato de titânio, isto pode ser decorrente da adesão do óxido de titânio na superfície da caulinita. Pela análise de EDS foi possível determinar a porcentagem de Ti (m/m) nas amostras sintetizadas, sendo que seu valor ficou entre 2,03 e 4,16 %. As análises por ICP-OES indicam que as amostras apresentam de 2,65 a 4,07% de Ti e os dados do Bandgap variam de 1,69 a 3,44 eV para as amostras com e sem Ti em sua composição.Palavras-chave: Caulinita, metacaulinita, ativação ácida, Método dos Precursores Poliméricos, Dióxido de Titânio.

Sprouty4 Is an Endogenous Negative Modulator of TrkA Signaling and Neuronal Differentiation Induced by NGF

The Sprouty (Spry) family of proteins represents endogenous regulators of downstream signaling pathways induced by receptor tyrosine kinases (RTKs). Using real time PCR, we detect a significant increase in the expression of Spry4 mRNA in response to NGF, indicating that Spry4 could modulate intracellular signaling pathways and biological processes induced by NGF and its receptor TrkA. In this work, we demonstrate that overexpression of wild-type Spry4 causes a significant reduction in MAPK and Rac1 activation and neurite outgrowth induced by NGF. At molecular level, our findings indicate that ectopic expression of a mutated form of Spry4 (Y53A), in which a conserved tyrosine residue was replaced, fail to block both TrkA-mediated Erk/MAPK activation and neurite outgrowth induced by NGF, suggesting that an intact tyrosine 53 site is required for the inhibitory effect of Spry4 on NGF signaling. Downregulation of Spry4 using small interference RNA knockdown experiments potentiates PC12 cell differentiation and MAPK activation in response to NGF. Together, these findings establish a new physiological mechanism through which Spry4 regulates neurite outgrowth reducing not only the MAPK pathway but also restricting Rac1 activation in response to NGF

A breakthrough on Amanita phalloides poisoning: an effective antidotal effect by polymyxin B

Amanita phalloides is responsible for more than 90 % of mushroom-related fatalities, and no effective antidote is available. a-Amanitin, the main toxin of A. phalloides, inhibits RNA polymerase II (RNAP II), causing hepatic and kidney failure. In silico studies included docking and molecular dynamics simulation coupled to molecular mechanics with generalized Born and surface area method energy decomposition on RNAP II. They were performed with a clinical drug that shares chemical similarities to a-amanitin, polymyxin B. The results show that polymyxin B potentially binds to RNAP II in the same interface of a-amanitin, preventing the toxin from binding to RNAP II. In vivo, the inhibition of the mRNA transcripts elicited by a-amanitin was efficiently reverted by polymyxin B in the kidneys. Moreover, polymyxin B significantly decreased the hepatic and renal a-amanitin-induced injury as seen by the histology and hepatic aminotransferases plasma data. In the survival assay, all animals exposed to a-amanitin died within 5 days, whereas 50 % survived up to 30 days when polymyxin B was administered 4, 8, and 12 h post-a-amanitin. Moreover, a single dose of polymyxin B administered concomitantly with a-amanitin was able to guarantee 100 % survival. Polymyxin B protects RNAP II from inactivation leading to an effective prevention of organ damage and increasing survival in a-amanitin-treated animals. The present use of clinically relevant concentrations of an already human-use-approved drug prompts the use of polymyxin B as an antidote for A. phalloides poisoning in humans.Juliana Garcia, Vera Marisa Costa, Ricardo Dinis-Oliveira and Ricardo Silvestre thank FCT-Foundation for Science and Technology-for their PhD grant (SFRH/BD/74979/2010), Post-doc grants (SFRH/BPD/63746/2009 and SFRH/BPD/110001/2015) and Investigator grants (IF/01147/2013) and (IF/00021/2014), respectively. This work was supported by the Fundacao para a Ciencia e Tecnologia (FCT) - project PTDC/DTPFTO/4973/2014 - and the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundacao para a Ciencia e Tecnologia) through project Pest-C/EQB/LA0006/2013

Prenatal stress programs neuroendocrine stress responses and affective behaviors in second generation rats in a sex-dependent manner

AbstractAn adverse environment in early life is often associated with dysregulation of the hypothalamo-pituitary-adrenal (HPA) axis and higher rates of mood disorders in adulthood. In rats, exposure to social stress during pregnancy results in hyperactive HPA axis responses to stress in the adult offspring and heightened anxiety behavior in the males, but not the females. Here we tested whether, without further intervention, the effects of prenatal stress (PNS) in the first filial generation (F1) are transmitted to the F2 generation via the maternal line. F1 control and PNS female rats were mated with control males and housed under non-stress conditions throughout pregnancy. HPA axis responses to acute stress, anxiety- and depressive-like behavior were assessed in the adult F2 offspring.ACTH and corticosterone responses to an acute stressor were markedly enhanced in F2 PNS females compared with controls. This was associated with greater corticotropin releasing hormone (Crh) mRNA expression in the paraventricular nucleus and reduced hippocampal glucocorticoid (Gr) and mineralocorticoid receptor (Mr) mRNA expression. Conversely, in the F2 PNS males, HPA axis responses to acute stress were attenuated and hippocampal Gr mRNA expression was greater compared with controls.F2 PNS males exhibited heightened anxiety-like behavior (light-dark box and elevated plus maze) compared with F2 control males. Anxiety-like behavior did not differ between F2 control and PNS females during metestrus/diestrus, however at proestrus/estrus, F2 control females displayed a reduction in anxiety-like behavior, but this effect was not observed in the F2 PNS females. Heightened anxiety in the F2 PNS males was associated with greater Crh mRNA expression in the central nucleus of the amygdala compared with controls. Moreover, Crh receptor-1 (Crhr1) mRNA expression was significantly increased, whereas Crhr2 mRNA was significantly decreased in discrete regions of the amygdala in F2 PNS males compared with controls, with no differences in the F2 females. No differences in depressive-like behavior (sucrose preference or forced swim test) were observed in either sex. In conclusion, the effects of maternal stress during pregnancy on HPA axis regulation and anxiety-like behavior can be transmitted to future generations in a sex-dependent manner. These data have implications for human neuropsychiatric disorders with developmental origins

The bii4africa dataset of faunal and floral population intactness estimates across Africa’s major land uses

Sub-Saharan Africa is under-represented in global biodiversity datasets, particularly regarding the impact of land use on species’ population abundances. Drawing on recent advances in expert elicitation to ensure data consistency, 200 experts were convened using a modified-Delphi process to estimate ‘intactness scores’: the remaining proportion of an ‘intact’ reference population of a species group in a particular land use, on a scale from 0 (no remaining individuals) to 1 (same abundance as the reference) and, in rare cases, to 2 (populations that thrive in human-modified landscapes). The resulting bii4africa dataset contains intactness scores representing terrestrial vertebrates (tetrapods: ±5,400 amphibians, reptiles, birds, mammals) and vascular plants (±45,000 forbs, graminoids, trees, shrubs) in sub-Saharan Africa across the region’s major land uses (urban, cropland, rangeland, plantation, protected, etc.) and intensities (e.g., large-scale vs smallholder cropland). This dataset was co-produced as part of the Biodiversity Intactness Index for Africa Project. Additional uses include assessing ecosystem condition; rectifying geographic/ taxonomic biases in global biodiversity indicators and maps; and informing the Red List of Ecosystems