Control Systems and Ornamental Lighting. A Case Study: Illumination of the Facade of Santiago Hospital in Úbeda (Jaén)

We propose the analysis of the control systems associated with ornamental lighting applied to the architectural and monumental heritage from our teaching experience developed since 2017 at the University of Córdoba—Illumination Faculty of Philosophy of Córdoba, year 2017, and Center of Experimental Culture in the Plaza de la Corredera, year 2018—and the lighting project of the facade of the Hospital de Santiago de Úbeda, Jaén. The DMX system is the one which mostly fulfill the requirements of this kind of lighting. Its implementation is increasingly simple and is associated in many cases with the type of luminaire to be used and the particularity of differential wiring. This solution allows a personalized, creative, and dynamic control and the adaptation of the daily lighting to different events of singular character. It can be used as an instrument of economic revitalization of the tourist and commercial sectors, and of diffusion of the architectural and historical heritage values. The application of the Internet of Things (IoT) and Big Data could allow the creation of a predictive model that helps the spectral design of light sources and an objective tool that contributes to the confluence of art and science

Analysis of Outdoor Lighting Control Systems Applied to the New Smart City Models

Lighting accounts for more than 19% of the world’s electricity consumption. Simply replacing existing lighting systems with other LED technology would reduce energy consumption by up to 40%, and if we also use lighting controls, the figure can reach 80%. The transition to efficient lighting technologies (LEDs) is economically one of the most realistic and simple energy efficiency initiatives. Control systems play an important role in the world of lighting. Wherever you have exterior lighting, there will be a need for control. The systems that have been used so far have precedents that date back more than 35 years and allow control and monitoring functions of groups of light points, i.e. not individually. One of the major drawbacks of these systems is that they do not have flexibility, since they do not allow the individualization of the point of light, and in addition the orders that can emit are of generic character and affect the group, obtaining a rather inaccurate information of the installation. Complete telemanagement systems are currently being developed to meet the needs of different application segments. Experience shows that it is necessary to work with open systems so that the lighting management system works and communicates with other systems such as air treatment, safety systems, etc. Intelligent lighting, in addition to its control and energy management functions, also contributes to reducing the excess of artificial light to which our cities are subject, making them more livable

Mitochondrial DNA pathogenic mutations in multiple symmetric lipomatosis

The frequency of dermatological manifestations in diseases due to mitochondrial DNA mutations is not well known, although multiple symmetric lipomatosis has been repeatedly associated to mitochondrial DNA mutations. Here, we present a patient suffering from multiple symmetric lipomatosis and other skin signs. We found a new mitochondrial DNA mutation, m.8357T>C, in the tRNALys-coding gene and, using a cybrid approach, confirmed its pathogenicity. A meta-analysis of the dermatological signs of the patient shows that they are not common in patients with confirmed mitochondrial DNA mutations and suggests that, in these cases, lipomatosis is not related to the oxidative phosphorylation dysfunction, but to an alteration of an additional function associated to particular mitochondrial tRNAs

Oxidative phosphorylation dysfunction modifies the cell secretome

Mitochondrial oxidative phosphorylation disorders are extremely heterogeneous conditions. Their clinical and genetic variability makes the identification of reliable and specific biomarkers very challenging. Until now, only a few studies have focused on the effect of a defective oxidative phosphorylation functioning on the cell’s secretome, although it could be a promising approach for the identification and pre-selection of potential circulating biomarkers for mitochondrial diseases. Here, we review the insights obtained from secretome studies with regard to oxidative phosphorylation dysfunction, and the biomarkers that appear, so far, to be promising to identify mitochondrial diseases. We propose two new biomarkers to be taken into account in future diagnostic trials

Toxic and nutritional factors trigger Leber hereditary optic neuropathy due to a mitochondrial tRNA mutation

Leber hereditary optic neuropathy is a mitochondrial disease mainly due to pathologic mutations in mitochondrial genes related to the respiratory complex I of the oxidative phosphorylation system. Genetic, physiological, and environmental factors modulate the penetrance of these mutations. We report two patients suffering from this disease and harboring a m.15950G > A mutation in the mitochondrial DNA-encoded gene for the threonine transfer RNA. We also provide evidences supporting the pathogenicity of this mutation

A novel deep targeted sequencing method for minimal residual disease monitoring in acute myeloid leukemia

A high proportion of patients with acute myeloid leukemia who achieve minimal residual disease negative status ultimately relapse because a fraction of pathological clones remains undetected by standard methods. We designed and validated a high-throughput sequencing method for minimal residual disease assessment of cell clonotypes with mutations of NPM1, IDH1/2 and/or FLT3-single nucleotide variants. For clinical validation, 106 follow-up samples from 63 patients in complete remission were studied by sequencing, evaluating the level of mutations detected at diagnosis. The predictive value of minimal residual disease status by sequencing, multiparameter flow cytometry, or quantitative polymerase chain reaction analysis was determined by survival analysis. The sequencing method achieved a sensitivity of 10-4 for single nucleotide variants and 10-5 for insertions/deletions and could be used in acute myeloid leukemia patients who carry any mutation (86% in our diagnostic data set). Sequencing-determined minimal residual disease positive status was associated with lower disease-free survival (hazard ratio 3.4, P=0.005) and lower overall survival (hazard ratio 4.2, P<0.001). Multivariate analysis showed that minimal residual disease positive status determined by sequencing was an independent factor associated with risk of death (hazard ratio 4.54, P=0.005) and the only independent factor conferring risk of relapse (hazard ratio 3.76, P=0.012). This sequencing-based method simplifies and standardizes minimal residual disease evaluation, with high applicability in acute myeloid leukemia. It is also an improvement upon flow cytometry- and quantitative polymerase chain reaction-based prediction of outcomes of patients with acute myeloid leukemia and could be incorporated in clinical settings and clinical trials.This study was supported by the Subdirección General de Investigación Sanitaria (Instituto de Salud Carlos III, Spain) grants PI13/02387 and PI16/01530, and the CRIS against Cancer foundation grant 2014/0120. ML holds a postdoctoral fellowship of the Spanish Ministry of Economy and Competitiveness (FPDI-2013- 16409). PRP holds a postdoctoral fellowship of the Spanish Instituto de Salud Carlos III: Contrato Predoctoral de Formación en Investigación en Salud i-PFIS (IFI 14/00008).S

Novel deep targeted sequencing method for minimal residual disease monitoring in acute myeloid leukemia

A high proportion of patients with acute myeloid leukemia who achieve minimal residual disease (MRD) negative status ultimately relapse because a fraction of pathological clones remains undetected by standard methods. We designed and validated a high-throughput sequencing method for MRD assessment of cell clonotypes with mutations of NPM1, IDH1/2 and/or FLT3-SNVs. For clinical validation, 106 follow-up samples from 63 patients in complete remission were studied by NGS, evaluating the level of mutations detected at diagnosis. The predictive value of MRD status by NGS, multiparameter flow cytometry, or quantitative PCR was determined by survival analysis. The method achieved a sensitivity of 10-4 for SNV mutations and 10-5 for insertions/deletions and could be used in acute myeloid leukemia patients who carry any mutation (86% in our diagnosis data set). NGS-determined MRD positive status was associated with lower disease-free survival (hazard ratio [HR] 3.4, p=0.005) and lower overall survival (HR 4.2, p<0.001). Multivariate analysis showed that MRD positive status by NGS was an independent factor associated with risk of death (HR 4.54, p =0.005) and the only independent factor conferring risk of relapse (HR 3.76, p =0.012). This NGS based method simplifies and standardizes MRD evaluation, with high applicability in acute myeloid leukemia. It also improves upon flow cytometry and quantitative PCR to predict acute myeloid leukemia outcome and could be incorporated in clinical settings and clinical trials.This study was supported by the Subdirección General de Investigación Sanitaria (Instituto de Salud Carlos III, Spain) grants PI13/02387 and PI16/01530, and the CRIS against Cancer foundation grant 2014/0120. M.L. holds a postdoctoral fellowship of the Spanish Ministry of Economy and Competitiveness (FPDI-2013-16409). P.R.P. holds a postdoctoral fellowship of the Spanish of Instituto de Salud Carlos III: Contrato Predoctoral de Formación en Investigación en Salud i-PFIS (IFI 14/00008).S

Analysis of the effect of ultrasound on Hymenaea courbaril L. seeds / Análise do efeito do ultrassom em sementes de Hymenaea courbaril L.

Hymenaea courbaril L. popularly known as Jatobá is a native species of the Amazon. We selected 100 seeds for the morphometric analysis, for the water content of the seeds, four samples of 5 g each were used, for the germination test, four groups of seeds were evaluated: control group without ultrasound application and three other groups that were submitted to ultrasound application for 2 minutes (U2), 3 minutes (U3) and 4 minutes (U4) at a frequency of 3 MHz and an intensity of 2 W/cm² of exposure. Each group divided into four repetitions of 25 seeds each, were transferred to an incubator (B.O.D.) with a photoperiod of 12 h of light per day and 100% relative humidity, each group being repeated twice and tested at two temperatures (30 °C and 35 °C). The seed has 9.36% fresh mass, 23.62 mm, 14.1 mm, 65.59 mm in length, width, and perimeter respectively and 79.03% purity. The ultrasound waves applied for 3 minutes favored seed germination of 86.12% (30 ºC) and 83.04% (35 ºC) and mean germination time of  21.75 days (30°C) and 21.81 days (35°C) for two and three minutes respectively.  Therefore, the ultrasound technique is considered useful and promising tool for breaking tegumentary dormancy in Jatobá seeds. 

Colombian consensus recommendations for diagnosis, management and treatment of the infection by SARS-COV-2/ COVID-19 in health care facilities - Recommendations from expert´s group based and informed on evidence

La Asociación Colombiana de Infectología (ACIN) y el Instituto de Evaluación de Nuevas Tecnologías de la Salud (IETS) conformó un grupo de trabajo para desarrollar recomendaciones informadas y basadas en evidencia, por consenso de expertos para la atención, diagnóstico y manejo de casos de Covid 19. Estas guías son dirigidas al personal de salud y buscar dar recomendaciones en los ámbitos de la atención en salud de los casos de Covid-19, en el contexto nacional de Colombia