Interactions of PatA with the divisome during heterocyst differentiation in anabaena

The Anabaena organismic unit is a filament of communicating cells. Under conditions of nitrogen scarcity, some cells along the filament differentiate into heterocysts, which are specialized in the fixation of atmospheric N2 and provide the vegetative cells with N2 fixation products. At a certain stage, the differentiation process becomes irreversible, so that even when nitrogen is replenished, no return to the vegetative cell state takes place, possibly as a consequence of loss of cell division capacity. Upon N-stepdown, midcell FtsZ-rings were detected in vegetative cells, but not in differentiating cells, and this was also the case for ZipN, an essential protein that participates in FtsZ tethering to the cytoplasmic membrane and divisome organization. Later, expression of ftsZ was arrested in mature heterocysts. PatA is a protein required for the differentiation of intercalary heterocysts in Anabaena. The expression level of the patA gene was increased in differentiating cells, and a mutant strain lacking PatA exhibited enhanced FtsZ-rings. PatA was capable of direct interactions with ZipN and SepF, another essential component of the Anabaena Z-ring. Thus, PatA appears to promote inhibition of cell division in the differentiating cells, allowing progress of the differentiation process. PatA, which in mature heterocysts was detected at the cell poles, could interact also with SepJ, a protein involved in production of the septal junctions that provide cell-cell adhesion and intercellular communication in the filament, hinting at a further role of PatA in the formation or stability of the intercellular structures that are at the basis of the multicellular character of Anabaena

El bilingüismo guaraní castellano y su incidencia en la producción escrita de los alumnos al final del primer ciclo de la EEB

La presente tesis muestra un estudio sobre las características de la producción escrita bilingüe castellano-guaraní en niños de tercer grado de la Educación escolar básica en el Paraguay y los desafíos que esta situación plantea para la intervención docente en aulas con diversidad lingüística.Facultad de Humanidades y Ciencias de la Educació

Statistical models to study subtoxic concentrations for some standard mutagens in three colon cancer cell lines

The aim of this work is to propose models to study the toxic effect of different concentrations of some standard mutagens in different colon cancer cell lines. We find estimates and, by means of an inverse regression problem, confidence intervals for the subtoxic concentration, that is the concentration that reduces by thirty percent the number of colonies obtained in the absence of mutagen

Seminarios wiki interactivos de Derecho Internacional Privado con complemento-refuerzo de seminarios presenciales impartidos por especialistas en los temas propuestos

Memoria ID-0020. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2015-2016

Seminarios wiki interactivos de Derecho Internacional Privado con complemento-refuerzo de seminarios presenciales impartidos por especialistas en los temas propuestos

Memoria ID-0155. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2014-2015

Possible role of WDR3 gene on genome stability in thyroid cancer patients

The role of the WDR3 gene on genomic instability has been evaluated in a group of 115 differentiated thyroid cancer (DTC) patients. Genomic instability has been measured according to the response of peripheral blood lymphocytes to ionizing radiation (0.5 Gy). The response has been measured with the micronucleus (MN) test evaluating the frequency of binucleated cells with MN (BNMN), both before and after the irradiation. No differences between genotypes, for the BNMN frequencies previous the irradiation, were observed. Nevertheless significant decreases in DNA damage after irradiation were observed in individuals carrying the variant alleles for each of the three genotyped SNPs: rs3754127 [−8.85 (−15.01 to −2.70), P<0.01]; rs3765501 [−8.98 (−15.61 to −2.36), P<0.01]; rs4658973 [−8.70 (−14.94 to −2.46), P<0.01]. These values correspond to those obtained assuming a dominant model. This study shows for the first time that WDR3 can modulate genome stability

Expectativas laborales y potencial directivo en estudiantes de Licenciatura en Turismo

El propósito del estudio fue diagnosticar las expectativas, prioridades laborales y potencial directivo de un grupo de estudiantes de primer año de la  carrera de Licenciatura en Turismo de la Universidad de Camagüey Ignacio Agramonte Loynaz, Cuba. Además, se establecieron grupos focales de desarrollo. Fueron aplicados dos cuestionarios y analizados los datos con el paquete estadístico Statgraphics Centurion VXI y sus técnicas de análisis descriptivo. Fue posible identificar que en todos los estudiantes las expectativas laborales son inferiores al potencial directivo. Los principales aspectos en los que debe influirse son: las habilidades comunicativas, la profesionalidad, la vocación de directivo, el interés y dinamismo, la autoactualización, las relaciones con personas y la creatividad laboral. Son propuestos tres grupos de trabajo con 24; 21 y 5 individuos de acuerdo a las características expresadas en los tests. A partir de herramientas gráficas se muestran las posiciones relativas de los grupos y los individuos, lo que junto a la información que les da origen será relevante para plantear una futura etapa de investigación donde se diseñen y evalúen programas complementarios a la formación de pregrado

Long-term effects of polystyrene nanoplastics in human intestinal Caco-2 cells

The increasing presence of micro- and nanoplastics (MNPLs) in the environment, and their consequent accumulation in trophic niches, could pose a potential health threat to humans, especially due to their chronic ingestion. In vitro studies using human cells are considered pertinent approaches to determine potential health risks to humans. Nevertheless, most of such studies have been conducted using short exposure times and high concentrations. Since human exposure to MNPLs is supposed to be chronic, there is a lack of information regarding the potential in vitro MNPLs effects under chronic exposure conditions. To this aim, we assessed the accumulation and potential outcomes of polystyrene nanoparticles (PSNPs), as a model of MNPLs, in undifferentiated Caco-2 cells (as models of cell target in ingestion exposures) under a relevant long-term exposure scenario, consisting of eight weeks of exposure to sub-toxic PSNPs concentrations. In such exposure conditions, culture-media was changed every 2-3 days to maintain constant exposure. The different analyzed endpoints were cytotoxicity, dysregulation of stress-related genes, genotoxicity, oxidative DNA damage, and intracellular ROS levels. These are endpoints that showed to be sensitive enough in different studies. The obtained results attest that PSNPs accumulate in the cells through time, inducing changes at the ultrastructural and molecular levels. Nevertheless, minor changes in the different evaluated genotoxicity-related biomarkers were observed. This would indicate that no DNA damage or oxidative stress is observed in the human intestinal Caco-2 cells after long-term exposure to PSNPs. This is the first study dealing with the long-term effects of PSNPs on human cultured cells

Laboratorio de jurisprudencia de derecho internacional privado

Memoria ID12-0068. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2012-2013

Laboratorio de jurisprudencia de Derecho Internacional Privado

Memoria ID-095. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014