Analyses of regulatory CD4(+)CD25(+)FOXP3(+) T cells and observations from peripheral T cell subpopulation markers during the development of type 1 diabetes in children

Our aim was to study whether the aberrant amount or function of regulatory T cells is related to the development of type 1 diabetes (T1D) in children. We also set out to investigate the balance of different T cell subtype markers during the T1D autoimmune process. Treg cells were quantified with flow cytometric assay, and the suppression capacity was analysed with a carboxyfluorescein succinimidyl ester (CFSE)-based T cell suppression assay in children in various phases of T1D disease process and in healthy autoantibody-negative control children. The mRNA expression of different T cell subpopulation markers was analysed with real-time qPCR method. The proportion and suppression capacity of regulatory T cells were similar in seroconverted children at an early stage of beta cell autoimmunity and also in children with T1D when compared to healthy and autoantibody-negative children. Significant differences were observed in the mRNA expression of different T cell subpopulation markers in prediabetic children with multiple (2) autoantibodies and in children with newly diagnosed T1D when compared to the control children. In conclusion, there were no quantitative or functional differences in regulatory T cells between the case and control groups in any phase of the autoimmune process. Decreased mRNA expression levels of T cell subtype markers were observed in children with multiple islet autoantibodies and in those with newly diagnosed T1D, probably reflecting an exhaustion of the immune system after the strong immune activation during the autoimmune process or a generally aberrant immune response related to the progression of the disease.Peer reviewe

A piecewise-linear reduced-order model of squeeze-film damping for deformable structures including large displacement effects

This paper presents a reduced-order model for the Reynolds equation for deformable structure and large displacements. It is based on the model established in [11] which is piece-wise linearized using two different methods. The advantages and drawbacks of each method are pointed out. The pull-in time of a microswitch is determined and compared to experimental and other simulation data.Comment: Submitted on behalf of EDA Publishing Association (http://irevues.inist.fr/handle/2042/16838

Smoking in pregnancy, adolescent mental health and cognitive performance in young adult offspring: results from a matched sample within a Finnish cohort

$\textbf{Background:}$ The association between prenatal exposure to maternal cigarette smoking (PEMCS) and adult cognition is debated, including if there are differences according to sex. We aimed to determine if there are associations between PEMCS and cognition in early adulthood in men and women and examine if observed associations were mediated by adolescent mental health factors that are associated with cognition, namely psychotic-like experiences (PLEs), inattention and hyperactivity, and other externalizing behaviors. $\textbf{Methods:}$ Participants were 471 individuals drawn from the general population-based Northern Finland 1986 Birth Cohort (NFBC 1986) followed up from pregnancy and birth to early adulthood; individuals with PEMCS were matched with those without PEMCS by socioeconomic and demographic factors. Cognitive performance in adulthood was assessed with a range of tests and their association with PEMCS was measured by sex using hierarchical linear regression, unadjusted and then controlling for potential confounders, mediators and moderators, including adolescent mental health factors. $\textbf{Results:}$ There were no associations between PEMCS and cognitive scores in females. In males, there were associations with vocabulary (beta = -0.444, 95% CI: -0.783, -0.104) and matrix reasoning (beta = -0.379, 95% CI: -0.711, -0.047). $\textbf{Conclusions:}$ While associations between PEMCS and cognition were limited, observed findings with measures of general intelligence in males contribute to suggestions of differences in response to PEMCS by sex. Furthermore, observed associations may be partly mediated by earlier inattention and hyperactivity. Findings add support to efforts aimed to eliminate smoking in pregnancy.The Northern Finland Birth Cohort 1986 is funded by the University of Oulu, University Hospital of Oulu, Academy of Finland, Sigrid Juselius Foundation, European Commission (EURO-BLCS, Framework 5 award QLG1-CT-2000- 01643), and NIH/NIMH (5R01MH63706:02). Cambridge Cognition Ltd. provided support in the form of salaries for author Jennifer H. Barnett

History teaching in Finnish general upper secondary schools: Objectives and practices

In Finland, the trend towards a new kind of history teaching emphasizing the understanding of historical knowledge and historical thinking skills began in the mid-1990s, when history teaching objectives were defined much more broadly in the curriculum than previously. In this article, we examine how, in over twenty years since the changes in curriculum objectives were made, general upper secondary school teachers have come to value the curriculum objectives of history teaching and how these have impacted on their teaching. The data for this article were collected by a semi-structured survey in 2016. Using counts, percentages, means, standard deviations and medians, a descriptive exploration was made of history teachers’ perceptions of the essential objectives in teaching history and how often they were put into practice in related student activities. To investigate the balance between the objectives the teachers emphasized as the most essential and the teaching methods they actually used, we applied the Kruskal–Wallis test and the Friedman test. According to the results, what the teachers considered essential for teaching history did not correlate with their teaching methods. In addition, according to the results, this state of affairs is still undergoing change; old traditions and new objectives of history teaching are creating tensions. The results were interpreted in the light of the cultural viewpoints of Finnish teaching, the position of matriculation examinations in Finnish general upper secondary schools and the challenges the curriculum is setting for history teacher

A micro-accelerometer MDO benchmark problem

Many optimization and coordination methods for multidisciplinary design optimization (MDO) have been proposed in the last three decades. Suitable MDO benchmark problems for testing and comparing these methods are few however. This article presents a new MDO benchmark problem based on the design optimization of an ADXL150 type lateral capacitive micro-accelerometer. The behavioral models describe structural and dynamic effects, as well as electrostatic and amplification circuit contributions. Models for important performance indicators such as sensitivity, range, noise, and footprint area are presented. Geometric and functional constraints are included in these models to enforce proper functioning of the device. The developed models are analytical, and therefore highly suitable for benchmark and educational purposes. Four different problem decompositions are suggested for four design cases, each of which can be used for testing MDO coordination algorithms. As a reference, results for an all-in-one implementation, and a number of augmented Lagrangian coordination algorithms are given. © 2009 The Author(s)

Role of the electro-thermo-mechanical multiple coupling on the operation of RF microswitch

A phenomenological approach is proposed to identify some effects occurring within the structure of the microswitch conceived for radio frequency application. This microsystem is operated via a nonlinear electromechanical action imposed by the applied voltage. Unfortunately, it can be affected by residual stress, due to the microfabrication process, therefore axial and flexural behaviors are strongly coupled. This coupling increases the actuation voltage required to achieve the so-called ‘‘pull-in'' condition. Moreover, temperature may strongly affect strain and stress distributions, respectively. Environmental temperature, internal dissipation of material, thermo-elastic and Joule effects play different roles on the microswitch flexural isplacement. Sometimes buckling phenomenon evenly occurs. Literature show that all those issues make difficult an effective computation of ‘‘pull-in'' and ‘‘pull-out'' voltages for evenly distinguishing the origin of some failures detected in operation. Analysis, numerical methods and experiments are applied to an industrial test case to investigate step by step the RF-microswitch operation. Multiple electro-hermomechanical coupling is first modeled to have a preliminary and comprehensive description of the microswitch behavior and of its reliability. ‘‘Pull-in'' and ‘‘pull-out'' tests are then performed to validate the proposed models and to find suitable criteria to design the RF-MEM

The social affordances of flashpacking: exploring the mobility nexus of travel and communication

The proliferation of digital devices and online social media and networking technologies has altered the backpacking landscape in recent years. Thanks to the ready availability of online communication, travelers are now able to stay in continuous touch with friends, family and other travelers while on the move. This article introduces the practice of ‘flashpacking’ to describe this emerging trend and interrogates the patterns of connection and disconnection that become possible as corporeal travel and social technologies converge. Drawing on the concepts of ‘assemblages’ and ‘affordances’, we outline several aspects of this new sociality: virtual mooring, following, collaborating, and (dis)connecting. The conclusion situates this discussion alongside broader questions about the shifting nature of social life in an increasingly mobile and mediated world and suggests directions for future research at the intersection of tourism and technology

Simulations of Aerodynamic Damping for MEMS Resonators

Aerodynamic damping for MEMS resonators is studied based on the numerical solution of Boltzmann-ESBGK equation. A compact model is then developed based on numerical simulations for a wide range of Knudsen numbers. The damping predictions are compared with both Reynold equation based models and several sets of experimental data. It has been found that the structural damping is dominant at low pressures (high Knudsen numbers). For cases with small length-to-width ratios and large vibration amplitudes, the threedimensionality effects must be taken into account. Finally, an uncertainty quantification approach based on the probability transformation method has been applied to assess the influence of pressure and geometric uncertainties. The output probability density functions (PDF) of the damping ratio has been studied for various input PDF of beam geometry and ambient pressure

Analyses of regulatory CD4+CD25+FOXP3+ T cells and observations from peripheral T cell subpopulation markers during the development of type 1 diabetes in children

Our aim was to study whether the aberrant amount or function of regulatory T cells is related to the development of type 1 diabetes (T1D) in children. We also set out to investigate the balance of different T cell subtype markers during the T1D autoimmune process. Treg cells were quantified with flow cytometric assay, and the suppression capacity was analysed with a carboxyfluorescein succinimidyl ester (CFSE)-based T cell suppression assay in children in various phases of T1D disease process and in healthy autoantibody-negative control children. The mRNA expression of different T cell subpopulation markers was analysed with real-time qPCR method. The proportion and suppression capacity of regulatory T cells were similar in seroconverted children at an early stage of beta cell autoimmunity and also in children with T1D when compared to healthy and autoantibody-negative children. Significant differences were observed in the mRNA expression of different T cell subpopulation markers in prediabetic children with multiple (≥2) autoantibodies and in children with newly diagnosed T1D when compared to the control children. In conclusion, there were no quantitative or functional differences in regulatory T cells between the case and control groups in any phase of the autoimmune process. Decreased mRNA expression levels of T cell subtype markers were observed in children with multiple islet autoantibodies and in those with newly diagnosed T1D, probably reflecting an exhaustion of the immune system after the strong immune activation during the autoimmune process or a generally aberrant immune response related to the progression of the disease.</p