Fluorescent paper-based sensor integrated with headspace thin-film microextraction for the detection of acyclic N-nitrosamines following in situ photocatalytic decomposition

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGBackground: In this work, a novel analytical approach based on the photocatalytic decomposition of N-nitrosamines combined with headspace thin-film microextraction of the generated nitrogen oxides such as NO has been developed for the determination of the acyclic N-nitrosamine fraction in drinking water samples. A hydrophilic cellulose substrate modified with fluorescent silver nanoclusters (Ag NCs) was used both as extractant and sensing platform. A quenching effect of Ag NCs fluorescence occurs as the concentration of N-nitrosamines increases. Front-face fluorescence spectroscopy with a solid sample holder was employed for directly measuring the fluorescence quenching onto the cellulose substrate. Results: In order to achieve an optimal analytical response, different parameters involved in the photocatalytic reaction as well as those concerning the microextraction step were fully investigated. It is demonstrated that the photodegradation rate of cyclic N-nitrosamines at acidic pH is much lower than that of acyclic ones, which can be the basis for the determination of the later fraction in waters. Under optimal conditions, a detection limit for the acyclic N-nitrosamine fraction around 0.08 μg L−1 using N-nitrosodimethylamine (NDMA) as model compound for calibration was obtained. Several drinking waters were spiked with acyclic N-nitrosamines showing recoveries in the range of 98–102% with a relative standard deviation of 3–4% (N = 3). Significance and novelty: N-nitrosamines generated as by-products during disinfection processes applied to water cause multiple adverse effects on human health being classified as potential human carcinogens. This study highlights the suitability of a fluorescent paper-based sensor for the rapid analysis of the acyclic N-nitrosamine fraction (i.e. the most abundant fraction) as a total index in drinking water, being useful as screening tool before exhaustive chromatographic analysis, which saves costs, time and reduces waste generation.Agencia Estatal de Investigacion | Ref. RTI2018- 093697-B-I00Xunta de Galicia | Ref. ED481D-2021-02

Systematic Analysis of Transrectal Prostate Biopsies Using an Ink Method and Specific Histopathologic Protocol: A Prospective Study

Background. Transrectal prostate biopsy is the standard protocol for the screening for prostate cancer. It helps to locate prostatic adenocarcinoma and plan treatment. However, the increasing number of prostate biopsies leads to considerably greater costs for the pathology laboratories. In this study, we compare the traditional method with an ink method in combination with a systematic histopathologic protocol. Methods. Two hundred consecutive transrectal prostate biopsy specimens were received from the radiology department. They were separated into two groups: one hundred were processed as six different specimens in the usual manner. The other one hundred were submitted in six containers, the apex, base, and middle section of which were stained different colours. The samples subject to the ink method were embedded in paraffin and placed in two cassettes which were sectioned using a specific protocol. Results. The comparative study of the nonink and ink methods for histopathologic diagnosis showed no statistical differences as far as diagnostic categories were concerned (P  value < .005). The number of PIN diagnoses increased when the ink method was used, but no statistical differences were found. The ink method led to a cost reduction of 48.86%. Conclusions. Our ink method combined with a specific histopathologic protocol provided the same diagnostic quality, tumor location information as the traditional method, and lower pathology expenses

ER-bound PTP1B is targeted to newly forming cell-matrix adhesions

Here, we define the mechanism through which protein tyrosine phosphatase 1B (PTP1B) is targeted to cell-matrix adhesion sites. Green fluorescent protein (GFP)-labeled PTP1B bearing the substrate-trapping mutation D181A was found in punctate structures in lamellae. The puncta co-localized with focal adhesion kinase (FAK) and Src, and defined the distal tips of cell-matrix adhesion sites identified with paxillin and vinculin. PTP1B is largely associated with the external face of the endoplasmic reticulum (ER) and the puncta develop from ER projections over cell-matrix adhesion sites, a process dependent on microtubules. Deletion of the ER-targeting sequence resulted in cytosolic localization and altered the distribution of PTP1B at cell-matrix foci, whereas mutations disrupting interactions with Src homology 3 (SH3) domains, and the insulin and cadherin receptors had no effect. PTP1B recognizes substrates within forming adhesion foci as revealed by its preferential association with paxillin as opposed to zyxin-containing foci. Our results suggest that PTP1B targets to immature cell-matrix foci in newly forming lamellae by dynamic extensions of the ER and contributes to the maturation of these sites.Fil: Hernandez, Mariana Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Davies Sala, María Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Balsamo, Janne. University of Iowa; Estados UnidosFil: Lilien, Jack. University of Iowa; Estados UnidosFil: Arregui, Carlos Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentin

Directrices para la creación de documentos digitales accesibles en entornos de investigación, transferencia y difusión científica: el caso de la Red CDPD

La Red Iberoamericana de Expertos sobre la Convención de los Derechos de las Personas con Discapacidad es una organización con fines académicos y sociales que tiene como misión el estudio, aplicación y seguimiento de la Convención Internacional sobre los Derechos de las Personas con Discapacidad. En este contexto surge la necesidad de establecer una normativa de trabajo para la gestión de la comunicación científica que allí se produce. El presente artículo define los principales conceptos en materia de accesibilidad y acceso a la información, y delimita las directrices para la creación de documentos digitales accesibles en entornos de investigación, transferencia y difusión científica y su aplicación en la Red CDPD. PALABRAS claves: documentos digitales, accesibilidad,  investigación ,  transferencia, convención sobre los derechos de las personas con discapacidad Guidelines to create accessible digital documents in research environments, transfer and scientific diffusion: the case of the CRPD NetworkABSTRACT: The Latin American Network of Experts on the Convention on the Rights of Persons with Disabilities is an organization for academic and social purposes whose mission is the study, implementation and monitoring of the Convention on the Rights of Persons with Disabilities. In this context arises the necessity to establish a working rules for the management of scientific communication that is produced there. This article defines the main concepts on accessibility and access to information, and delimits the guidelines for creating accessible digital documents in research environments, transfer and scientific dissemination and application in the CDPD network. KEYWORDS: digital documents, accessibility, research, transference, Convention on the Rights of Persons with Disabilities. doi: http://dx.doi.org/10.20318/universitas.2016.3180

Rehabilitacion del equipo ensayo de presión hidrostática sobre superficies planas y curvas sumergidas y fuerzas de boyamiento sobre un cuerpo parcialmente sumergido

La materia se clasifica, en sólidos y fluidos; En un sentido estricto, se puede considerar un fluido como un conjunto de moléculas distribuidas al azar que se mantienen unidas a través de fuerzas cohesivas débiles y las fuerzas ejercidas por las paredes del recipiente que lo contiene. La rama de la física que estudia los fluidos, recibe el nombre de mecánica de los fluidos, la cual a su vez tiene dos vertientes: hidrostática, que orienta su atención a los fluidos en reposo; e hidrodinámica, la cual envuelve los fluidos en movimiento. La hidrostática es la parte de la hidráulica que estudia el equilibrio de los liquidas en estado de reposo. En estas circunstancias, al ser nulo el gradiente de velocidad, no existen esfuerzos cortantes (tangenciales), por lo que no existe viscosidad comportándose el líquido como perfecto. Por tanto, pueden obtenerse sus leyes de forma analítica, no siendo necesario recurrir a la experimentación para corregir las ecuaciones con coeficientes que ajusten la teoría a la realidad. Respecto a la segunda parte del laboratorio, donde la fuerza resultante ejercida sobre un cuerpo por un fluido estático, que se encuentra sumergido o flotando se conoce como la fuerza de Boyamiento. Ésta siempre actúa verticalmente hacia arriba. No puede existir componente horizontal de la resultante debido a que la proyección del cuerpo sumergido o la porción sumergida de un cuerpo flotante sobre un plano vertical siempre es cero. La fuerza de Boyamiento sobre un cuerpo sumergido es la diferencia entre la componente vertical de la fuerza de presión en su lado superior y la componente vertical de la fuerza de presión en su lado inferio

Acute otitis media in infants younger than three months not vaccinated against Streptococcus pneumoniae

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Effects of preeclampsia and eclampsia on maternal metabolic and biochemical outcomes in later life: a systematic review and meta-analysis

Objective: To evaluate the association between preeclampsia (PE) and eclampsia (E) on subsequent metabolic and biochemical outcomes. Methods: Systematic review and meta-analysis of observational studies. We searched five engines until November 2018 for studies evaluating the effects of PE/E on metabolic and biochemical outcomes after delivery. PE was defined as presence of hypertension and proteinuria at >20 weeks of pregnancy; controls did not have PE/E. Primary outcomes were blood pressure (BP), body mass index (BMI), metabolic syndrome (MetS), blood lipids and glucose levels. Random effects models were used for meta-analyses, and effects reported as risk difference (RD) or mean difference (MD) and their 95% confidence interval (CI). Subgroup analyses by time of follow up, publication year, and confounder adjustment were performed. Results: We evaluated 41 cohorts including 3300 PE/E and 13,967 normotensive controls. Women were followed up from 3 months after delivery up to 32 years postpartum. In comparison to controls, PE/E significantly increased systolic BP (MD = 8.3 mmHg, 95%CI 6.8 to 9.7), diastolic BP (MD = 6.8 mmHg, 95%CI 5.6 to 8.0), BMI (MD = 2.0 kg/m2; 95%CI 1.6 to 2.4), waist (MD = 4.3 cm, 95%CI 3.1 to 5.5), waist-to-hip ratio (MD = 0.02, 95%CI 0.01 to 0.03), weight (MD = 5.1 kg, 95%CI 2.2 to 7.9), total cholesterol (MD = 4.6 mg/dL, CI 1.5 to 7.7), LDL (MD = 4.6 mg/dL; 95%CI 0.2 to 8.9), triglycerides (MD = 7.7 mg/dL, 95%CI 3.6 to 11.7), glucose (MD = 2.6 mg/dL, 95%CI 1.2 to 4.0), insulin (MD = 19.1 pmol/L, 95%CI 11.9 to 26.2), HOMA-IR index (MD = 0.7, 95%CI 0.2 to 1.2), C reactive protein (MD = 0.05 mg/dL, 95%CI 0.01 to 0.09), and the risks of hypertension (RD = 0.24, 95%CI 0.15 to 0.33) and MetS (RD = 0.11, 95%CI 0.08 to 0.15). Also, PE/E reduced HDL levels (MD = –2.15 mg/dL, 95%CI –3.46 to −0.85). Heterogeneity of effects was high for most outcomes. Risk of bias was moderate across studies. Subgroup analyses showed similar effects as main analyses. Conclusion: Women who had PE/E have worse metabolic and biochemical profile than those without PE/E in an intermediate to long term follow up period. ©Revisión por pare

Comparative study of nomophobia among Spanish and Portuguese nursing students

Nomophobia is the fear of leaving the house without a mobile and being out of mobile phone contact and affects different areas of a person's life, especially in terms of social, work and academic relationships due to a dependence on the use of smartphones. Discovering the prevalence of nomophobia among nursing students is very important, as the misuse of smartphones in clinical practice may cause distractions, affecting the quality of care and putting patient safety at risk. Furthermore, it can lead to poorer academic performance during class. The aim of this study was to compare the levels of nomophobia experienced by nursing students at the University of Almeria, Spain and the Polytechnic Institute of Braganza, Portugal. A comparative descriptive observational study was carried out. A nomophobia questionnaire adapted to the Spanish and Portuguese sociolinguistic context was employed; 258 participants comprised the subjects of study. The main results showed both Spanish and Portuguese nursing students scored higher than average regarding levels of nomophobia. However, the scores gathered from items on the questionnaire were generally higher among the Portuguese population than the Spanish one. The Portuguese students (54.7%) felt more anxious than the Spanish students (35.4%) if their battery ran out. Similarly, the Portuguese population showed a greater need for instant communication with their family and friends. In conclusion, the dimensions explored indicate significant levels of nomophobia among both nursing student populations, with higher levels among the Portuguese population than the Spanish.info:eu-repo/semantics/publishedVersio

Criterios de Implementación ISO 14000:2015 Caso de Estudio Sector Avícola

La actividad avícola en Colombia, se ha venido desarrollando de manera favorable en los últimos años, en la actualidad la avicultura ha ido en aumento, en el año 2019 la avicultura colombiana como tal ha tenido un crecimiento de 0,7, donde la producción de pollo y huevo se ha incrementado en 1,9% y 1,6% respectivamente, en relación con el año anterior (2018). En relación al crecimiento para la avicultura colombiana, Gonzalo Moreno indicó para el 2020 se prevé un crecimiento de 3% (FENAVI). En el Valle del Cauca, existe una gran cantidad de industrias avícolas, enfocándose en la producción de huevo, cría, levante y engorde de pollo. La industria avícola requiere de la implementación de nuevas prácticas ambientales sostenibles, que permitan mejorar y optimizar las condiciones de producción y manejo, con el fin de mitigar el impacto ambiental y lograr un desarrollo económico satisfactorio sin afectar al medio ambiente. A partir de ello se escogió la Granja Avícola Santanita Nápoles SA, ubicada en Buga Valle del Cauca. En la presente auditoria se identificó el estado actual de la empresa. Se realizó una evaluación minuciosa de las instalaciones, los impactos ambientales generados por la actividad productiva y el cumplimiento de requisitos legales normativos y políticas ambientales dentro de la misma para lo cual se establecieron los criterios de aspectos ambientales de la empresa. Como auditores luego de la realización de la auditoria tenemos responsabilidad de expresar opiniones sobre el estado ambiental de la empresa Avícola, con la finalidad de aportar a la mejora continua de la organización. Desde la reunión de apertura hasta el cierre de la auditoría, existirá la confidencialidad, objetividad e imparcialidad necesaria para cumplir con los objetivos propuestos y del mismo modo informar de forma adecuada los hallazgos, conformidades, las no conformidades y las conclusiones.Poultry farming in Colombia has been developing favorably in recent years, currently poultry farming has been increasing, in 2019 Colombian poultry farming as such has had a growth of 0.7, where the production of chicken and egg has increased by 1.9% and 1.6% respectively, in relation to the previous year (2018). In relation to the growth for Colombian poultry, Gonzalo Moreno indicated for 2020 a growth of 3% is expected (FENAVI). In Valle del Cauca, there are a large number of poultry industries, focusing on egg production, breeding, raising and chicken fattening. The poultry industry requires the implementation of new sustainable environmental practices that allow the improvement and optimization of production and handling conditions, in order to mitigate environmental impact and achieve satisfactory economic development without affecting the environment. Based on this, the Santanita Napoles SA Poultry Farm, located in Buga Valle del Cauca, was chosen. In this audit, the current state of the company was identified. A meticulous evaluation of the facilities was carried out, the environmental impacts generated by the productive activity and the fulfillment of regulatory legal requirements and environmental policies within the same, for which the criteria of environmental aspects of the company were established. As auditors, after conducting the audit, we are responsible for expressing opinions on the environmental status of the Avícola company, in order to contribute to the continuous improvement of the organization. From the opening meeting to the closing of the audit, there will be the confidentiality, objectivity and impartiality necessary to meet the proposed objectives and in the same way adequately report the findings, conformities, non-conformities and conclusion

Detection of gases and organic vapors by cellulose-based sensors

The growing interest in the development of cost-effective, straightforward, and rapid analytical systems has found cellulose-based materials, including cellulose derivatives, cellulose-based gels, nanocellulosic materials, and the corresponding (nano)cellulose-based composites, to be valuable platforms for sensor development. The present work presents recent advances in the development of cellulose-based sensors for the determination of volatile analytes and derivatives of analytical relevance. In particular, strategies described in the literature for the fabrication and modification of cellulose-based substrates with responsive materials are summarized. In addition, selected contributions reported in the field of paper-based volatile sensors are discussed, with a particular emphasis on quick response (QR) code paper-based platforms, intelligent films for food freshness monitoring, and sensor arrays for volatile discrimination purposes. Furthermore, analytical strategies devised for the determination of ionic species by in situ generation of volatile derivatives in both paper-based analytical devices (PADs) and microfluidic PADs will also be described.Universidade de Vigo/CISUGAgencia Estatal de Investigación | Ref. RTI2018-093697-B-I0