PREVALENCE OF HYPERTENSION AND DIABETES MELLITUS AMONG INDIGENOUS PEOPLES

Objective: to analyze the prevalence of systemic hypertension and diabetes mellitus among indigenous villagers associated with ethnicity and describe the frequency of care/diagnosis according to professional category.Method: epidemiological and descriptive study, carried out with data on Systemic Hypertension and Diabetes Mellitus produced in the Distritos Sanitários Especiais Indígenas (Special Indigenous Health Districts of Pará), between 2013-2017, obtained from the Sistema de Informações da Atenção à Saúde Indígena (Indigenous Health Care Information System). For analysis, the morbidities weregrouped, and Pearson’s Chi-square was used, p≤0.05.Results: 624 cases of Systemic Arterial Hypertension and 108 cases of Diabetes mellitus were studied, identifying a greater involvement of women. The Munduruku ethnic group showed a higher prevalence of systemic hypertension (35.0%; n=219) and diabetes mellitus (23.1%; n=25). It was observed expressive participation of the nursing team in the care of indigenous peoples.Conclusion: The identified prevalence can be attributed to the accelerated nutritional transition and changes in lifestyle habits. Such findings are important for qualified and culturally meaningful nursing care.Objetivo: analisar prevalência de Hipertensão Arterial Sistêmica e Diabetes mellitus nos indígenas aldeados associadas à etnia e descrever a frequência de atendimento/diagnóstico segundo categoria profissional.Método: estudo epidemiológico, descritivo, realizado com dados de Hipertensão Arterial Sistêmica e Diabetes mellitus produzidos nos Distritos Sanitários Especiais Indígenas, do Pará, entre 2013-2017, obtidos do Sistema de Informações da Atenção à Saúde Indígena. Para análise, agrupou-se as morbidades e utilizou-se o Qui-quadrado de Pearson, p≤0,05.Resultados: foram estudados 624 casos de Hipertensão Arterial Sistêmica e 108 casos de Diabetes mellitus, identificando-se maior acometimento de mulheres. A etnia Munduruku apresentou maior prevalência de Hipertensão Arterial Sistêmica (35,0%; n=219) e Diabetes mellitus (23,1%; n=25). Observou-se participação expressiva da equipe de enfermagem no atendimento aos indígenas.Conclusão: A prevalência identificada pode ser atribuída à acelerada transição nutricional e mudanças nos hábitos de vida. Tais achados são importantes para assistência de enfermagem qualificada e culturalmente significada.Objetivo: analizar la prevalencia de Hipertensión Arterial Sistémica y de la Diabetes mellitus en pobladores indígenas asociada a la etnia y describir la frecuencia de atención/diagnóstico según la categoría profesional. Método: estudio epidemiológico y descriptivo, realizado con datos de hipertensión arterial sistémica y diabetes mellitus producidos en los Distritos Especiales de Salud Indígena del Estado de Pará, entre 2013-2017, obtenidos del Sistema de Información de Atención a la Salud Indígena. Para el análisis, se agruparon las morbilidades y se utilizó la prueba de chi-cuadradode Pearson, p≤0,05. Resultados: Se estudiaron 624 casos de Hipertensión Arterial Sistémica y 108 casos de Diabetes mellitus, identificando una mayor afectación de las mujeres. El grupo étnico Munduruku mostró una mayor prevalencia de hipertensión sistémica (35,0%; n=219) y de diabetes mellitus (23,1%; n=25). Se observó la participación expresiva del equipo de enfermería en el cuidado de los indígenas. Conclusión: La prevalencia identificada puede atribuirse a la transición nutricional acelerada y a los cambios en los hábitos de vida. Estos resultados son importantes para una atención de enfermería cualificada y culturalmente significativa

Use of BODIPY-Labeled ATP Analogues in the Development and Validation of a Fluorescence Polarization-Based Assay for Screening of Kinase Inhibitors

Copyright © 2020 American Chemical Society. The screening of compound libraries to identify small-molecule modulators of specific biological targets is crucial in the process for the discovery of novel therapeutics and molecular probes. Considering the need for simple single-tool assay technologies with which one could monitor "all" kinases, we developed a fluorescence polarization (FP)-based assay to monitor the binding capabilities of protein kinases to ATP. We used BODIPY ATP-y-S as a probe to measure the shift in the polarization of a light beam when passed through the sample. We were able to optimize the assay using commercial Protein Kinase A (PKA) and H7 efficiently inhibited the binding of the probe when added to the reaction. Furthermore, we were able to employ the assay in a high-throughput fashion and validate the screening of a set of small molecules predicted to dock into the ATP-binding site of PKA. This will be useful to screen larger libraries of compounds that may target protein kinases by blocking ATP binding

GESTÃO FAMILIAR COMO FATOR DE INFLUÊNCIA NO NÍVEL DE DISCLOSURE DE ATIVOS INTANGÍVEIS

The aim of this study is to observe the differences in disclosure levels of intangible assets between family businesses and unfamiliar listed on the BM&FBovespa in front of the mandatory application of Technical Pronouncement CPC 04 (R1) - Intangible Assets. Entities were identified whose control was familiar through the Reference Form analysis of all 525 companies listed on the BM&FBovespa. Next, we proceeded to the analysis of their balance sheets and Notes for the construction of grip index to CPC 04(R1) through the check list structure din Avelino, Pinho and Lamounier (2012) research. Data were analyzed using descriptive and inferential statistics with the aid of Stata software. The hypothesis that the level of disclosure of intangible assets of family businesses is lower when compared to non-family companies was formulated. From the results, we could not reject the null hypothesis of this study, finding that there are no significant differences between the disclosure levels of the bodies of the two segments analyzed. However, we found statistically significant differences in the levels of disclosure relating to spending on research and development recognized as expenses.O objetivo principal deste estudo é discutir as diferenças nos níveis de evidenciação dos ativos intangíveis entre as empresas familiares e não familiares listadas na BM&FBovespa, diante da obrigatoriedade da aplicação do Pronunciamento Técnico CPC 04 (R1) – Ativos Intangíveis. Foram identificadas as entidades cujo controle fosse familiar por meio da análise do Formulário de Referência de todas as 525 empresas listadas na BM&FBovespa. Na sequência, procedeu-se à análise de seus Balanços Patrimoniais e Notas Explicativas para a construção do índice de aderência ao CPC 04 (R1), por meio do checklist estruturado na pesquisa de Avelino, Pinheiro e Lamounier (2012). Os dados coletados foram analisados por meio da estatística descritiva e inferencial com o auxílio do software Stata. Foi formulada a hipótese de que o nível de disclosure dos ativos intangíveis das empresas familiares é menor quando comparado ao das companhias não familiares. A partir dos resultados encontrados, não se pôde rejeitar a hipótese nula deste estudo, verificando que não há diferenças significativas entre os níveis de disclosure das entidades dos dois segmentos analisados. Entretanto, foram encontradas diferenças estatisticamente relevantes nos níveis de divulgação que dizem respeito aos gastos com pesquisa e desenvolvimento reconhecidos como despesas

Novel Targeting of DNA Methyltransferase Activity Inhibits Ewing Sarcoma Cell Proliferation and Enhances Tumor Cell Sensitivity to DNA Damaging Drugs by Activating the DNA Damage Response

DNA methylation is an important component of the epigenetic machinery that regulates the malignancy of Ewing sarcoma (EWS), the second most common primary bone tumor in children and adolescents. Coordination of DNA methylation and DNA replication is critical for maintaining epigenetic programming and the DNMT1 enzyme has been demonstrated to have an important role in both maintaining the epigenome and controlling cell cycle. Here, we showed that the novel nonnucleoside DNMT inhibitor (DNMTi) MC3343 induces a specific depletion of DNMT1 and affects EWS tumor proliferation through a mechanism that is independent on DNA methylation. Depletion of DNMT1 causes perturbation of the cell cycle, with an accumulation of cells in the G1 phase, and DNA damage, as revealed by the induction of gamma H2AX foci. These effects elicited activation of p53-dependent signaling and apoptosis in p53wt cells, while in p53 mutated cells, persistent micronuclei and increased DNA instability was observed. Treatment with MC3343 potentiates the efficacy of DNA damaging agents such as doxorubicin and PARP-inhibitors (PARPi). This effect correlates with increased DNA damage and synergistic tumor cytotoxicity, supporting the use of the DNMTi MC3343 as an adjuvant agent in treating EWS

“Coisa de menina” e “coisa de menino”? Uma leitura do preconceito de gênero pela perspectiva dos praticantes de balé clássico masculino e futebol feminino

Um reflexo do machismo estrutural na sociedade é a criação de estereótipos de gênero, que determinam o que é aceitável para homens ou mulheres, estigmatizando certas práticas corporais. Este artigo buscou evidenciar os preconceitos sofridos por dois grupos – mulheres no futebol e homens no balé clássico – a partir de suas próprias percepções, através de um questionário com perguntas abertas e fechadas. A análise dos dados fez uso das categorias sociológicas apresentadas por Marchi Júnior, Almeida e Souza (2019), para explicar os fenômenos sociais e qualificar uma leitura preliminar do tema

A novel Modulator of Ring Stage Translation (MRST) gene alters artemisinin sensitivity in Plasmodium falciparum

The implementation of artemisinin (ART) combination therapies (ACTs) has greatly decreased deaths caused by Plasmodium falciparum malaria, but increasing ACT resistance in Southeast Asia and Africa could reverse this progress. Parasite population genetic studies have identified numerous genes, single-nucleotide polymorphisms (SNPs), and transcriptional signatures associated with altered artemisinin activity with SNPs in the Kelch13 (K13) gene being the most well-characterized artemisinin resistance marker. However, there is an increasing evidence that resistance to artemisinin in P. falciparum is not related only to K13 SNPs, prompting the need to characterize other novel genes that can alter ART responses in P. falciparum. In our previous analyses of P. falciparum piggyBac mutants, several genes of unknown function exhibited increased sensitivity to artemisinin that was similar to a mutant of K13. Further analysis of these genes and their gene co-expression networks indicated that the ART sensitivity cluster was functionally linked to DNA replication and repair, stress responses, and maintenance of homeostatic nuclear activity. In this study, we have characterized PF3D7_1136600, another member of the ART sensitivity cluster. Previously annotated as a conserved Plasmodium gene of unknown function, we now provide putative annotation of this gene as a Modulator of Ring Stage Translation (MRST). Our findings reveal that the mutagenesis of MRST affects gene expression of multiple translation-associated pathways during the early ring stage of asexual development via putative ribosome assembly and maturation activity, suggesting an essential role of MRST in protein biosynthesis and another novel mechanism of altering the parasite’s ART drug response

Chemogenomic profiling of a Plasmodium falciparum transposon mutant library reveals shared effects of dihydroartemisinin and bortezomib on lipid metabolism and exported proteins

The antimalarial activity of the frontline drug artemisinin involves generation of reactive oxygen species (ROS) leading to oxidative damage of parasite proteins. To achieve homeostasis and maintain protein quality control in the overwhelmed parasite, the ubiquitin-proteasome system kicks in. Even though molecular markers for artemisinin resistance like pfkelch13 have been identified, the intricate network of mechanisms driving resistance remains to be elucidated. Here, we report a forward genetic screening strategy that enables a broader identification of genetic factors responsible for altering sensitivity to dihydroartemisinin (DHA) and a proteasome inhibitor, bortezomib (BTZ). Using a library of isogenic piggyBac mutants in P. falciparum, we defined phenotype-genotype associations influencing drug responses and highlighted shared mechanisms between the two processes, which mainly included proteasome-mediated degradation and the lipid metabolism genes. Additional transcriptomic analysis of a DHA/BTZ-sensitive piggyBac mutant showed it is possible to find differences between the two response mechanisms on the specific components for regulation of the exportome. Our results provide further insight into the molecular mechanisms of antimalarial drug resistance

Phenotypic screens identify genetic factors associated with gametocyte development in the human malaria parasite Plasmodium falciparum

Transmission of the deadly malaria parasite Plasmodium falciparum from humans to mosquitoes is achieved by specialized intraerythrocytic sexual forms called gametocytes. Though the crucial regulatory mechanisms leading to gametocyte commitment have recently come to light, networks of genes that control sexual development remain to be elucidated. Here, we report a pooled-mutant screen to identify genes associated with gametocyte development in P. falciparum. Our results categorized genes that modulate gametocyte progression as hypoproducers or hyperproducers of gametocytes, and the in-depth analysis of individual clones confirmed phenotypes in sexual commitment rates and putative functions in gametocyte development. We present a new set of genes that have not been implicated in gametocytogenesis before and demonstrate the potential of forward genetic screens in isolating genes impacting parasite sexual biology, an exciting step toward the discovery of new antimalarials for a globally significant pathogen

Multicentre multi-device hybrid imaging study of coronary artery disease: results from the EValuation of INtegrated Cardiac Imaging for the Detection and Characterization of Ischaemic Heart Disease (EVINCI) hybrid imaging population

AIMS: Hybrid imaging provides a non-invasive assessment of coronary anatomy and myocardial perfusion. We sought to evaluate the added clinical value of hybrid imaging in a multi-centre multi-vendor setting. METHODS AND RESULTS: Fourteen centres enrolled 252 patients with stable angina and intermediate (20-90%) pre-test likelihood of coronary artery disease (CAD) who underwent myocardial perfusion scintigraphy (MPS), CT coronary angiography (CTCA), and quantitative coronary angiography (QCA) with fractional flow reserve (FFR). Hybrid MPS/CTCA images were obtained by 3D image fusion. Blinded core-lab analyses were performed for CTCA, MPS, QCA and hybrid datasets. Hemodynamically significant CAD was ruled-in non-invasively in the presence of a matched finding (myocardial perfusion defect co-localized with stenosed coronary artery) and ruled-out with normal findings (both CTCA and MPS normal). Overall prevalence of significant CAD on QCA (>70% stenosis or 30-70% with FFR 640.80) was 37%. Of 1004 pathological myocardial segments on MPS, 246 (25%) were reclassified from their standard coronary distribution to another territory by hybrid imaging. In this respect, in 45/252 (18%) patients, hybrid imaging reassigned an entire perfusion defect to another coronary territory, changing the final diagnosis in 42% of the cases. Hybrid imaging allowed non-invasive CAD rule-out in 41%, and rule-in in 24% of patients, with a negative and positive predictive value of 88% and 87%, respectively. CONCLUSION: In patients at intermediate risk of CAD, hybrid imaging allows non-invasive co-localization of myocardial perfusion defects and subtending coronary arteries, impacting clinical decision-making in almost one every five subjects