Paleoclimate in continental northwestern Europe during the Eemian and early Weichselian (125–97 ka): insights from a Belgian speleothem

The last interglacial serves as an excellent time interval for studying climate dynamics during past warm periods. Speleothems have been successfully used for reconstructing the paleoclimate of last interglacial continental Europe. However, all previously investigated speleothems are restricted to southern Europe or the Alps, leaving large parts of northwestern Europe undocumented. To better understand regional climate changes over the past, a larger spatial coverage of European last interglacial continental records is essential, and speleothems, because of their ability to obtain excellent chronologies, can provide a major contribution. Here, we present new, high-resolution data from a stalagmite (Han-9) obtained from the Han-sur-Lesse Cave in Belgium. Han-9 formed between 125.3 and  ∼  97 ka, with interruptions of growth occurring at 117.3–112.9 and 106.6–103.6 ka. The speleothem was investigated for its growth, morphology and stable isotope (δ13C and δ18O) composition. The speleothem started growing relatively late within the last interglacial, at 125.3 ka, as other European continental archives suggest that Eemian optimum conditions were already present during that time. It appears that the initiation of Han-9 growth is caused by an increase in moisture availability, linked to wetter conditions around 125.3 ka. The δ13C and δ18O proxies indicate a period of relatively stable conditions after 125.3 ka; however, at 120 ka the speleothem δ18O registered the first signs of regionally changing climate conditions, being a modification of ocean source δ18O linked to an increase in ice volume towards the Marine Isotope Stage (MIS) 5e–5d transition. At 117.5 ka, drastic vegetation changes are recorded by Han-9 δ13C immediately followed by a cessation of speleothem growth at 117.3 ka, suggesting a transition to significantly dryer conditions. The Han-9 record covering the early Weichselian displays larger amplitudes in both isotope proxies and changes in stalagmite morphology, evidencing increased variability compared to the Eemian. Stadials that appear to be analogous to those in Greenland are recognized in Han-9, and the chronology is consistent with other European (speleothem) records. Greenland Stadial 25 is reflected as a cold/dry period within Han-9 stable isotope proxies, and the second interruption in speleothem growth occurs simultaneously with Greenland Stadial 24

Reconstruction of MIS 5 climate in the central Levant using a stalagmite from Kanaan Cave, Lebanon

Lying at the transition between the temperate Mediterranean domain and subtropical deserts, the Levant is a key area to study the palaeoclimatic response over glacial–interglacial cycles. This paper presents a precisely dated last interglacial (MIS 5) stalagmite (129–84 ka) from the Kanaan Cave, Lebanon. Variations in growth rate and isotopic records indicate a warm humid phase at the onset of the last interglacial at ~ 129 ka that lasted until ~ 125 ka. A gradual shift in speleothem isotopic composition (125–122 ka) is driven mainly by the δ18O source effect of the eastern Mediterranean surface waters during sapropel 5 (S5). The onset of glacial inception began after ~ 122 ka, interrupted by a short wet pulse during the sapropel 4 (S4) event. Low growth rates and enriched oxygen and carbon values until ~ 84 ka indicate a transition to drier conditions during Northern Hemisphere glaciation

Reliability of Upper Limb Pin-Prick Stimulation With Electroencephalography: Evoked Potentials, Spectra and Source Localization

In order for electroencephalography (EEG) with sensory stimuli measures to be used in research and neurological clinical practice, demonstration of reliability is needed. However, this is rarely examined. Here we studied the test-retest reliability of the EEG latency and amplitude of evoked potentials and spectra as well as identifying the sources during pin-prick stimulation. We recorded EEG in 23 healthy older adults who underwent a protocol of pin-prick stimulation on the dominant and non-dominant hand. EEG was recorded in a second session with rest intervals of 1 week. For EEG electrodes Fz, Cz, and Pz peak amplitude, latency and frequency spectra for pin-prick evoked potentials was determined and test-retest reliability was assessed. Substantial reliability ICC scores (0.76–0.79) were identified for evoked potential negative-positive amplitude from the left hand at C4 channel and positive peak latency when stimulating the right hand at Cz channel. Frequency spectra showed consistent increase of low-frequency band activity (< 5 Hz) and also in theta and alpha bands in first 0.25 s. Almost perfect reliability scores were found for activity at both low-frequency and theta bands (ICC scores: 0.81–0.98). Sources were identified in the primary somatosensory and motor cortices in relation to the positive peak using s-LORETA analysis. Measuring the frequency response from the pin-prick evoked potentials may allow the reliable assessment of central somatosensory impairment in the clinical setting

Reliability of Upper Limb Pin-Prick Stimulation With Electroencephalography : Evoked Potentials, Spectra and Source Localization

In order for electroencephalography (EEG) with sensory stimuli measures to be used in research and neurological clinical practice, demonstration of reliability is needed. However, this is rarely examined. Here we studied the test-retest reliability of the EEG latency and amplitude of evoked potentials and spectra as well as identifying the sources during pin-prick stimulation. We recorded EEG in 23 healthy older adults who underwent a protocol of pin-prick stimulation on the dominant and non-dominant hand. EEG was recorded in a second session with rest intervals of 1 week. For EEG electrodes Fz, Cz, and Pz peak amplitude, latency and frequency spectra for pin-prick evoked potentials was determined and test-retest reliability was assessed. Substantial reliability ICC scores (0.76-0.79) were identified for evoked potential negative-positive amplitude from the left hand at C4 channel and positive peak latency when stimulating the right hand at Cz channel. Frequency spectra showed consistent increase of low-frequency band activity (< 5 Hz) and also in theta and alpha bands in first 0.25 s. Almost perfect reliability scores were found for activity at both low-frequency and theta bands (ICC scores: 0.81-0.98). Sources were identified in the primary somatosensory and motor cortices in relation to the positive peak using s-LORETA analysis. Measuring the frequency response from the pin-prick evoked potentials may allow the reliable assessment of central somatosensory impairment in the clinical setting

Influence of production variables and starting material on charcoal stable isotopic and molecular characteristics

We present a systematic study on the effect of starting species, gas composition, temperature, particle size and duration of heating upon the molecular and stable isotope composition of high density (mangrove) and low density (pine) wood. In both pine and mangrove, charcoal was depleted in o13C relative to the starting wood by up to 1.6&#37; and 0.8&#37;, respectively. This is attributed predominantly to the progressive loss of isotopically heavier polysaccharides, and kinetic effects of aromatization during heating. However, the pattern of o13C change was dependant upon both starting species and atmosphere, with different structural changes associated with charcoal production from each wood type elucidated by Solid-State o13C Nuclear Magnetic Resonance Spectroscopy. These are particularly evident at lower temperatures, where variation in the oxygen content of the production atmosphere results in differences in the thermal degradation of cellulose and lignin. It is concluded that production of charcoal from separate species in identical conditions, or from a single sample exposed to different production variables, can result in significantly different o13C of the resulting material, relative to the initial wood. These results have implications for the use of charcoal isotope composition to infer past environmental change

Relationship of admission blood proteomic biomarkers levels to lesion type and lesion burden in traumatic brain injury: a CENTER-TBI study

Background: We aimed to understand the relationship between serum biomarker concentration and lesion type and volume found on computed tomography (CT) following all severities of TBI. Methods: Concentrations of six serum biomarkers (GFAP, NFL, NSE, S100B, t-tau and UCH-L1) were measured in samples obtained <24 hours post-injury from 2869 patients with all severities of TBI, enrolled in the CENTER-TBI prospective cohort study (NCT02210221). Imaging phenotypes were defined as intraparenchymal haemorrhage (IPH), oedema, subdural haematoma (SDH), extradural haematoma (EDH), traumatic subarachnoid haemorrhage (tSAH), diffuse axonal injury (DAI), and intraventricular haemorrhage (IVH). Multivariable polynomial regression was performed to examine the association between biomarker levels and both distinct lesion types and lesion volumes. Hierarchical clustering was used to explore imaging phenotypes; and principal component analysis and k-means clustering of acute biomarker concentrations to explore patterns of biomarker clustering. Findings: 2869 patient were included, 68% (n=1946) male with a median age of 49 years (range 2-96). All severities of TBI (mild, moderate and severe) were included for analysis with majority (n=1946, 68%) having a mild injury (GCS 13-15). Patients with severe diffuse injury (Marshall III/IV) showed significantly higher levels of all measured biomarkers, with the exception of NFL, than patients with focal mass lesions (Marshall grades V/VI). Patients with either DAI+IVH or SDH+IPH+tSAH, had significantly higher biomarker concentrations than patients with EDH. Higher biomarker concentrations were associated with greater volume of IPH (GFAP, S100B, t-tau;adj r2 range:0·48-0·49; p<0·05), oedema (GFAP, NFL, NSE, t-tau, UCH-L1;adj r2 range:0·44-0·44; p<0·01), IVH (S100B;adj r2 range:0.48-0.49; p<0.05), Unsupervised k-means biomarker clustering revealed two clusters explaining 83·9% of variance, with phenotyping characteristics related to clinical injury severity. Interpretation: Interpretation: Biomarker concentration within 24 hours of TBI is primarily related to severity of injury and intracranial disease burden, rather than pathoanatomical type of injury

Reliability of upper limb pin-prick stimulation with electroencephalography : evoked potentials, spectra and source localization

In order for electroencephalography (EEG) with sensory stimuli measures to be used in research and neurological clinical practice, demonstration of reliability is needed. However, this is rarely examined. Here we studied the test-retest reliability of the EEG latency and amplitude of evoked potentials and spectra as well as identifying the sources during pin-prick stimulation.We recorded EEG in 23 healthy older adults who underwent a protocol of pin-prick stimulation on the dominant and non-dominant hand. EEG was recorded in a second session with rest intervals of 1 week. For EEG electrodes Fz, Cz, and Pz peak amplitude, latency and frequency spectra for pin-prick evoked potentials was determined and test-retest reliability was assessed. Substantial reliability ICC scores (0.76–0.79) were identified for evoked potential negative-positive amplitude from the left hand at C4 channel and positive peak latency when stimulating the right hand at Cz channel. Frequency spectra showed consistent increase of low-frequency band activity (< 5 Hz) and also in theta and alpha bands in first 0.25 s. Almost perfect reliability scores were found for activity at both low-frequency and theta bands (ICC scores: 0.81–0.98). Sources were identified in the primary somatosensory and motor cortices in relation to the positive peak using s-LORETA analysis. Measuring the frequency response from the pin-prick evoked potentials may allow the reliable assessment of central somatosensory impairment in the clinical setting.peer-reviewe