Comparison of two assays for human kallikrein 2

BACKGROUND: We compared two recently developed research assays for the measurement of human kallikrein 2 (hK2) in serum: one fully automated assay (Beckman Coulter Access immunoanalyzer) and one manual assay based on the DELFIA technology. METHODS: We used two subsets of clinical specimens consisting of 48 samples from prostate cancer patients and 210 samples from participants in an ongoing screening study (ERSPC). Both subsets were measured in the Rotterdam laboratory, and the prostate cancer samples were used for analytical comparison with the originating sites for the assays: Beckman Coulter Research Department (San Diego, CA) and Turku University (Turku, Finland). RESULTS: Both the Beckman Coulter and the Turku assays performed very similarly between the Rotterdam laboratory and the originating sites: the R(2) value for both comparisons was 0.99, and the slope difference between sites was <20%. Deming regression analysis of the DELFIA (y) and Access (x) assays yielded the following: for the prostate cancer group, y = 1.17x - 0.01 (R(2) = 0.88; n = 48); and for the ERSPC group, y = 0.62x - 0.01 (R(2) = 0.77). Breakdown of the latter group into subgroups (nondiseased, benign prostatic hyperplasia, and prostate cancer samples) gave only minor differences. The Access calibrators were underrecovered by 13% in the DELFIA assay, whereas the DELFIA calibrators were overrecovered by 45% in the Access assay. CONCLUSION: The DELFIA and Access assays for hK2, which have similar analytical features, show differences that cannot be explained by calibration

Optical Follow-up of New SMC Wing Be/X-ray Binaries

We investigate the optical counterparts of recently discovered Be/X-ray binaries in the Small Magellanic Cloud. In total four sources, SXP101, SXP700, SXP348 and SXP65.8 were detected during the Chandra Survey of the Wing of the SMC. SXP700 and SXP65.8 were previously unknown. Many optical ground based telescopes have been utilised in the optical follow-up, providing coverage in both the red and blue bands. This has led to the classification of all of the counterparts as Be stars and confirms that three lie within the Galactic spectral distribution of known Be/X-ray binaries. SXP101 lies outside this distribution becoming the latest spectral type known. Monitoring of the Halpha emission line suggests that all the sources bar SXP700 have highly variable circumstellar disks, possibly a result of their comparatively short orbital periods. Phase resolved X-ray spectroscopy has also been performed on SXP65.8, revealing that the emission is indeed harder during the passage of the X-ray beam through the line of sight.Comment: 9 pages, 9 figures, 2 tables, accepted for publication in MNRA

Near and mid-infrared colours of star-forming galaxies in ELAIS fields

We present J and K-band near-infrared photometry of a sample of mid-infrared sources detected by the Infrared Space Observatory (ISO) as part of the European Large Area ISO-Survey (ELAIS) and study their classification and star-forming properties. We have used the Preliminary ELAIS Catalogue for the 6.7 micron (LW2) and 15 micron (LW3) fluxes. All of the high-reliability LW2 sources and 80 per cent of the LW3 sources are identified in the near-IR survey reaching K = 17.5 mag. The near- to mid-IR flux ratios can effectively be used to separate stars from galaxies in mid-IR surveys. The stars detected in our survey region are used to derive a new accurate calibration for the ELAIS ISOCAM data in both the LW2 and LW3 filters. We show that near to mid-IR colour-colour diagrams can be used to further classify galaxies, as well as study star-formation. The ISOCAM ELAIS survey is found to mostly detect strongly star-forming late-type galaxies, possibly starburst powered galaxies, and it also picks out obscured AGN. The ELAIS galaxies yield an average mid-IR flux ratio LW2/LW3 = 0.67 +/- 0.27. We discuss this [6.7/15] ratio as a star formation tracer using ISO and IRAS data of a local comparison sample. We find that the [K/15] ratio is also agood indicator of activity level in galaxies and conclude that the drop in the [6.7/15] ratio seen in strongly star-forming galaxies is a result of both an increase of 15 mic emission and an apparent depletion of 6.7 mic emission. Near-IR data together with the mid-IR give the possibility to estimate the relative amount of interstellar matter in the galaxies.Comment: 18 pages, 15 figures; accepted for publication in MNRA

Properties of the solar neighbor WISE J072003.20-084651.2

The severe crowding towards the Galactic plane suggests that the census of nearby stars in that direction may be incomplete. Recently, Scholz reported a new M9 object at an estimated distance d~7 pc (WISE J072003.20-084651.2; hereafter WISE0720) at Galactic latitude b=2.3 degr. Our goals are to determine the physical characteristics of WISE0720, its kinematic properties, and to address the question if it is a binary object, as suggested in the discovery paper. Optical and infrared spectroscopy from the Southern African Large Telescope and Magellan, respectively, and spectral energy distribution fitting were used to determine the spectral type of WISE0720. The measured radial velocity, proper motion and parallax yielded its Galactic velocities. We also investigated if WISE0720 may show X-ray activity based on archival data. Our spectra are consistent with spectral type L0+/-1. We find no evidence for binarity, apart for a minor 2-sigma level difference in the radial velocities taken at two different epochs. The spatial velocity of WISE0720 does not connect it to any known moving group, instead it places the object with high probability in the old thin disk or in the thick disk. The spectral energy distribution fit hints at excess in the 12 and 22 micron WISE bands which may be due to a redder companion, but the same excess is visible in other late type objects, and it more likely implies a shortcoming of the models (e.g., issues with the effective wavelengths of the filters for these extremely cool objects, etc.) rather than a disk or redder companion. The optical spectrum shows some Halpha emission, indicative of stellar activity. Archival X-ray observations yield no detection.Comment: A&A, accepted; 9 pages, 6 figure

The XMM large scale structure survey: optical vs. X-ray classifications of active galactic nuclei and the unified scheme

Our goal is to characterize AGN populations by comparing their X-ray and optical classifications. We present a sample of 99 spectroscopically identified X-ray point sources in the XMM-LSS survey which are significantly detected in the [2-10] keV band, and with more than 80 counts. We performed an X-ray spectral analysis for all of these 99 X-ray sources. Introducing the fourfold point correlation coefficient, we find only a mild correlation between the X-ray and the optical classifications, as up to 30% of the sources have differing X-ray and optical classifications: on one hand, 10% of the type 1 sources present broad emission lines in their optical spectra and strong absorption in the X-rays. These objects are highly luminous AGN lying at high redshift and thus dilution effects are totally ruled out, their discrepant nature being an intrinsic property. Their X-ray luminosities and redshifts distributions are consistent with those of the unabsorbed X-ray sources with broad emission lines. On the other hand, 25/32 are moderate luminosity AGN, which are both unabsorbed in the X-rays and only present narrow emission lines in their optical spectra. The majority of them have an optical spectrum which is representative of the host galaxy. We finally infer that dilution of the AGN by the host galaxy seems to account for their nature. 5/25 have been defined as Seyfert 2. In conclusion, most of these 32 discrepant cases can be accounted for by the standard AGN unified scheme, as its predictions are not met for only 12% of the 99 X-ray sources. ABRIDGEDComment: 25 pages, 19 figures, Accepted for publication in A&